羊血和鸡血红细胞膜通透性的比较研究
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摘要
细胞膜是一种半透性膜,能选择性地控制物质进出,研究细胞膜的通透性对细胞病变,某些疾病的致病机理以及药物疗效上有重要意义。本研究以新鲜鸡血和羊血为研究材料,采用观察血细胞在不同等渗溶液中是否发生溶血并记录溶血时间方法来进行比较。将等渗溶液分为脂溶性物质,水溶性物质、水、强电解质、弱电解质几类进行分析,结果表明:鸡血红细胞膜的通透性低于羊血红细胞膜。造成两种红细胞膜之间通透性的差异主要原因是红细胞膜自身的机械强度,红细胞的生理特性,溶质的性质及血细胞来源等多方因素的影响。
Cell membrane is a semipermeable membrane. It could selectively control the entry and exit of substances. The study of the permeability of cell membranes has important meaning for the cell lesion, the pathogenesis of some diseases and the efficacy of drugs. In this study, we took fresh chicken blood and goat blood as research materials, and compared them by observing whether there was hemolysis in different isotonic solution and recorded the hemolysis time. We divided the isotonic solution into the fat soluble substances, water soluble substance, water, strong electrolyte and weak electrolyte types, and then analyzed. The results showed that the erythrocyte membrane permeability of chicken blood was lower than that of goat erythrocyte membrane. The difference between the permeability of the two erythrocyte membranes might be mainly due to the mechanical strength of the erythrocyte membrane, the physiological characteristics of the erythrocytes, the nature of the solute and the origin of the blood cells.
Cell membrane is a semipermeable membrane. It could selectively control the entry and exit of substances. The study of the permeability of cell membranes has important meaning for the cell lesion, the pathogenesis of some diseases and the efficacy of drugs. In this study, we took fresh chicken blood and goat blood as research materials, and compared them by observing whether there was hemolysis in different isotonic solution and recorded the hemolysis time. We divided the isotonic solution into the fat soluble substances, water soluble substance, water, strong electrolyte and weak electrolyte types, and then analyzed. The results showed that the erythrocyte membrane permeability of chicken blood was lower than that of goat erythrocyte membrane. The difference between the permeability of the two erythrocyte membranes might be mainly due to the mechanical strength of the erythrocyte membrane, the physiological characteristics of the erythrocytes, the nature of the solute and the origin of the blood cells.
引文
Cheng W.Y.,Yu G.H.,Chen Y.,Liu X.Q.,and Wang C.T.,2009,Result analysis of the experiment of red blood cell membrane permeability,Shiyankexue Yu Jishu(Experiment Science and Technology),7(5):39-41(程旺元,余光辉,陈雁,刘学群,王春台,2009,红细胞膜通透性研究及分析,实验科学与技术,7(5):39-41)
Liu Q.Y.,Ding L.,Gao Y.,and Zhu L.C.,1993,The research of solution affeetrion to transport and fragility of chickens'Erythroeyte,Anhui Daxue Xuebao(Journal of Anhui University(Natural Science Edition)),4(3):65-70(刘春云,丁亮,高宇,朱领成,1993,离体鸡血红细胞的脆性及通透性测定,安徽大学学报(自然科学版),4(3):65-70)
Ma C.Y.,Shi H.L.,and Chen C.,2006,The application of restoring thought on the experiment of cell membrane permeability,Tangshan Shifan Xueyuan Xuebao(Journal of Tangshan Teachers College),28(2):27-28(马超颖,石洪凌,陈超,2006,还原思维在“细胞膜的渗透性实验”中的应用,唐山师范学院学报,28(2):27-28)
Ma Y.,Liang X.J.,Liu Y.,Cheng Z.Z.,Dou J.L.,and Chen Z.E.,2010,The improvement of cell membrane permeability experiment and the extraction of DNA from chicken blood,Xinxiang Xueyuan Xuebao(Journal of Xinxiang University(Natural Science Edition)),27(6):43-46(马云,梁小娟,刘英,程珍珍,豆佳丽,陈志娥,2010,细胞膜渗透性实验的改进及鸡血液DNA的提取,新乡学院学报(自然科学版),27(6):43-46)
Sun Y.,and Hao M.P.,1998,Study on the permeability of cell membrane to ammonium,Hebei Shifan Daxue Xuebao(Journal of Hebei Normal University(Natural Science)),22(2):264-266(孙颖和郝美璞,1998,铵盐通透细胞膜机理的探讨,河北师范大学学报(自然科学版),22(2):264-266)
Xie H.A.,Liu C.Y.,and Zong W.M.,1996,The measurement of permeability and fragilty of human's erythrocyte in vitro,Anhui Daxue Xuebao(Journal of Anhui University(Natural Science Edition)),20(2):98-104(谢惠安,刘春云,宗文明,1996,离体人血红细胞的脆性及通透性测定,安徽大学学报(自然科学版),20(2):98-104)
X u W.P.,Jiang J.L.,Ren X.M.,Li L.,Li X.S.,and Zhao,H.,2017,Effect of low temperature stress on cellmembrane and osmoregulatory substance in three varieties of citrus reticulate blanco seedlings,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(3):1104-1108(徐卫平,蒋景龙,任绪明,李丽,李新生,赵桦,2017,低温胁迫对3种柑橘幼苗细胞膜及渗透调节的影响,分子植物育种,14(3):1104-1108)
Zhang X.N.,and Zhao J.H.,2015,Studies on influencing factors of membrane permeability,Zhoukou Shifan Xueyuan Xuebao(Journal of Zhou Kou Normal University),32(5):126-128(张喜纳和赵锦慧,2015,影响细胞膜通透性因素研究,周口师范学院学报,32(5):126-128)
Liu Q.Y.,Ding L.,Gao Y.,and Zhu L.C.,1993,The research of solution affeetrion to transport and fragility of chickens'Erythroeyte,Anhui Daxue Xuebao(Journal of Anhui University(Natural Science Edition)),4(3):65-70(刘春云,丁亮,高宇,朱领成,1993,离体鸡血红细胞的脆性及通透性测定,安徽大学学报(自然科学版),4(3):65-70)
Ma C.Y.,Shi H.L.,and Chen C.,2006,The application of restoring thought on the experiment of cell membrane permeability,Tangshan Shifan Xueyuan Xuebao(Journal of Tangshan Teachers College),28(2):27-28(马超颖,石洪凌,陈超,2006,还原思维在“细胞膜的渗透性实验”中的应用,唐山师范学院学报,28(2):27-28)
Ma Y.,Liang X.J.,Liu Y.,Cheng Z.Z.,Dou J.L.,and Chen Z.E.,2010,The improvement of cell membrane permeability experiment and the extraction of DNA from chicken blood,Xinxiang Xueyuan Xuebao(Journal of Xinxiang University(Natural Science Edition)),27(6):43-46(马云,梁小娟,刘英,程珍珍,豆佳丽,陈志娥,2010,细胞膜渗透性实验的改进及鸡血液DNA的提取,新乡学院学报(自然科学版),27(6):43-46)
Sun Y.,and Hao M.P.,1998,Study on the permeability of cell membrane to ammonium,Hebei Shifan Daxue Xuebao(Journal of Hebei Normal University(Natural Science)),22(2):264-266(孙颖和郝美璞,1998,铵盐通透细胞膜机理的探讨,河北师范大学学报(自然科学版),22(2):264-266)
Xie H.A.,Liu C.Y.,and Zong W.M.,1996,The measurement of permeability and fragilty of human's erythrocyte in vitro,Anhui Daxue Xuebao(Journal of Anhui University(Natural Science Edition)),20(2):98-104(谢惠安,刘春云,宗文明,1996,离体人血红细胞的脆性及通透性测定,安徽大学学报(自然科学版),20(2):98-104)
X u W.P.,Jiang J.L.,Ren X.M.,Li L.,Li X.S.,and Zhao,H.,2017,Effect of low temperature stress on cellmembrane and osmoregulatory substance in three varieties of citrus reticulate blanco seedlings,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(3):1104-1108(徐卫平,蒋景龙,任绪明,李丽,李新生,赵桦,2017,低温胁迫对3种柑橘幼苗细胞膜及渗透调节的影响,分子植物育种,14(3):1104-1108)
Zhang X.N.,and Zhao J.H.,2015,Studies on influencing factors of membrane permeability,Zhoukou Shifan Xueyuan Xuebao(Journal of Zhou Kou Normal University),32(5):126-128(张喜纳和赵锦慧,2015,影响细胞膜通透性因素研究,周口师范学院学报,32(5):126-128)