铯137辐照对库存红细胞表面分子CD35、CD47的影响
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摘要
目的探讨铯137(~(137)Cs)辐照对库存红细胞表面分子红细胞1型补体受体(complement receptor type1,CR1 or CD35)及CD47分子的影响。方法采集60例健康献血者静脉血,制备去白细胞的悬浮红细胞,并将其分为2组各30例,未辐照组常规4℃保存,辐照组采用25Gy~(137)Cs辐照后4℃保存。分别于第0、7、14、28、35天收集2mL,样本。采用直标法流式细胞术检测红细胞表面分子CD35、CD47。结果辐照组和未辐照组红细胞CD35、CD47的表达均随着4℃保存时间的延长而减少,2组的时点间以及组间·时点间交互作用比较差异均有统计学意义(P<0.01),但组间差异无统计学意义(P>0.05)。结论 25Gy~(137)Csγ射线辐照对保存期内红细胞CD35、CD47表面分子表达无明显影响。
Objective To investigate the effects of 25 Gy ~(137)Cs radiation on the red cell surface molecule complement receptor type 1(CD35) or CD35 and CD47 in storage erythrocytes.Methods The vein blood samples of healthy donors were collected,leukocyte-depleted suspended erythrocytes were prepared.The specimens of 60 cases were randomly divided into two groups with 30 cases in each group and stored at 4℃,namely non-irradiation group(control group) and irradiation group.The suspended erythrocytes(2mL) were respectively collected on the 0,7th,14 th,21st,28 th,and 35 th day after the storage of two groups.The quantity of CD35 and CD47 of erythrocytes were assessed by direct calibration method using flow cytometry.Results The quantity of erythrocytes CD35 and CD47 of non-irradiated group and irradiation group decreased during storage at 4 ℃.The difference had statistical significance in different storage times and interactions between groups and time(P< 0.01).However,intergroup difference was of no significance(P>0.05).Conclusion 25 Gy ~(137)Cs radiation does not have significant effects on the CD35 and CD47 expression.
Objective To investigate the effects of 25 Gy ~(137)Cs radiation on the red cell surface molecule complement receptor type 1(CD35) or CD35 and CD47 in storage erythrocytes.Methods The vein blood samples of healthy donors were collected,leukocyte-depleted suspended erythrocytes were prepared.The specimens of 60 cases were randomly divided into two groups with 30 cases in each group and stored at 4℃,namely non-irradiation group(control group) and irradiation group.The suspended erythrocytes(2mL) were respectively collected on the 0,7th,14 th,21st,28 th,and 35 th day after the storage of two groups.The quantity of CD35 and CD47 of erythrocytes were assessed by direct calibration method using flow cytometry.Results The quantity of erythrocytes CD35 and CD47 of non-irradiated group and irradiation group decreased during storage at 4 ℃.The difference had statistical significance in different storage times and interactions between groups and time(P< 0.01).However,intergroup difference was of no significance(P>0.05).Conclusion 25 Gy ~(137)Cs radiation does not have significant effects on the CD35 and CD47 expression.
引文
[1]PATIDAR GK,JOSHI A,MARWAHA N,et al.Serial assessment of biochemical changes in irradiated red blood cells[J].Transfus Apher Sci,2014,50(3):479-487.
[2]LAPIN ZJ,HOPPENER C,GELBARD HA,et al.Near-field quantification of complement receptor 1(CR1/CD35)protein clustering in humanerythrocytes[J].J Neuroimmune Pharmacol,2012,7(3):539-543.
[3]BURGER P,HILARIUS-STOKMAN P,DE KORTE D,et al.CD47 functions as a molecular switch for erythrocyte phagocytosis[J].Blood,2012,119(23):5512-5521.
[4]胡金川,田亚平,江朝光,等.直标法流式细胞术测定人红细胞表面CD35水平的方法学研究[J].标记免疫分析与临床,2008,15(6):396-399.
[5]SUNUL H,ERGUVEN N.Transfusion-associated graftversus-host disease[J],Transfus Apher Sci,2013,49(2):331-333.
[6]ZIMMERMANN R,SCHOETZ AM,FRISCH A,et al.Influence of late irradiation on the in vitro RBC storage variables of leucoreduced RBCs in SAGM additive solution[J].Vox Sang,2011,100(3):279-284.
[7]CREHAN H,HOLTON P,WRAY S,et al.Complement receptor 1(CR1)and Alzheimer's disease[J].Immunobiology,2012,217(2):244-250.
[8]李志强,徐文皓,乐嘉宜,等.~(60)Co辐照血红细胞CR1分子数目的变化[J].河南诊断与治疗杂志,2002,16(1):1-2.
[9]OLDENBORG PA.CD47:a cell surface glycoprotein which regulates multiple functions of hematopoietic cells in health and disease[J].ISRN Hematol,2013,2013:614619.
[10]ANNISS AM,SPARROW RL.Expression of CD47(integriassociated protein)decreases on red blood cells during storage[J].Transfus Apher Sci,2002,27(3):233-238.
[2]LAPIN ZJ,HOPPENER C,GELBARD HA,et al.Near-field quantification of complement receptor 1(CR1/CD35)protein clustering in humanerythrocytes[J].J Neuroimmune Pharmacol,2012,7(3):539-543.
[3]BURGER P,HILARIUS-STOKMAN P,DE KORTE D,et al.CD47 functions as a molecular switch for erythrocyte phagocytosis[J].Blood,2012,119(23):5512-5521.
[4]胡金川,田亚平,江朝光,等.直标法流式细胞术测定人红细胞表面CD35水平的方法学研究[J].标记免疫分析与临床,2008,15(6):396-399.
[5]SUNUL H,ERGUVEN N.Transfusion-associated graftversus-host disease[J],Transfus Apher Sci,2013,49(2):331-333.
[6]ZIMMERMANN R,SCHOETZ AM,FRISCH A,et al.Influence of late irradiation on the in vitro RBC storage variables of leucoreduced RBCs in SAGM additive solution[J].Vox Sang,2011,100(3):279-284.
[7]CREHAN H,HOLTON P,WRAY S,et al.Complement receptor 1(CR1)and Alzheimer's disease[J].Immunobiology,2012,217(2):244-250.
[8]李志强,徐文皓,乐嘉宜,等.~(60)Co辐照血红细胞CR1分子数目的变化[J].河南诊断与治疗杂志,2002,16(1):1-2.
[9]OLDENBORG PA.CD47:a cell surface glycoprotein which regulates multiple functions of hematopoietic cells in health and disease[J].ISRN Hematol,2013,2013:614619.
[10]ANNISS AM,SPARROW RL.Expression of CD47(integriassociated protein)decreases on red blood cells during storage[J].Transfus Apher Sci,2002,27(3):233-238.