SDF1/CXCR4表达情况与胃癌患者临床特征的关系
|
摘要
目的探讨C-X-C家族趋化因子受体4(CXCR4)和基质细胞衍生因子1(SDF1)在胃癌中的表达情况及其临床意义。方法选取300例胃癌患者的病理组织标本,同时选取上述胃癌患者的癌旁组织、正常胃组织作为对照。采用免疫组织化学染色法检测CXCR4和SDF1蛋白的表达情况,采用实时逆转录聚合酶链反应(RTPCR)法检测CXCR4 mRNA和SDF1 m RNA的相对表达水平。结果胃癌组织和癌旁组织中的CXCR4蛋白阳性表达率和CXCR4 mRNA相对表达水平均高于正常胃组织,差异均有统计学意义(P﹤0.05)。正常胃组织、癌旁组织和胃癌组织中SDF1蛋白的阳性表达率和SDF1 m RNA相对表达水平比较,差异均无统计学意义(P﹥0.05)。不同临床分期和淋巴结转移胃癌患者胃癌组织中CXCR4蛋白阳性表达率和CXCR4 mRNA相对表达水平比较,差异均有统计学意义(P﹤0.05)。结论 CXCR4蛋白和CXCR4 mRNA相对表达水平在胃癌组织中明显升高,可能与胃癌患者的临床分期和淋巴结转移有关。
Objective To investigate the expression of C-X-C motif chemokine receptor 4(CXCR4) and stromal cellderived factor 1(SDF1) in gastric cancer,and to explore the clinical significance.Method A total of 300 patients with gastric cancer were included in the study for retrospective analysis,the gastric cancer tissues,adjacent tissues and normal gastric tissues were collected,the expression of CXCR4 and SDF1 were detected by immunohistochemical staining,and the relative expression of CXCR4 m RNA and SDF1 m RNA were detected by reverse transcription polymerase chain reaction(RT-PCR).Result The positive expression rates of CXCR4 protein and relative expression of CXCR4 mRNA in gastric cancer tissues and adjacent tissues were significantly higher than that in normal gastric tissues(P<0.05).There was no significant difference in the positive expression rate of SDF1 protein and the relative expression level of SDF1 mRNA between normal gastric tissue,adjacent tissue and gastric cancer tissue(P>0.05).While clinical staging and lymph node metastasis were associated with the positive expression of CXCR4 protein and the relative expression of CXCR4 mRNA,with statistically significant differences observed(P<0.05).Conclusion The expression of CXCR4 protein and CXCR4 mRNA in gastric cancer tissues are increased significantly,which may be related to the clinical stage and lymph node metastasis.
Objective To investigate the expression of C-X-C motif chemokine receptor 4(CXCR4) and stromal cellderived factor 1(SDF1) in gastric cancer,and to explore the clinical significance.Method A total of 300 patients with gastric cancer were included in the study for retrospective analysis,the gastric cancer tissues,adjacent tissues and normal gastric tissues were collected,the expression of CXCR4 and SDF1 were detected by immunohistochemical staining,and the relative expression of CXCR4 m RNA and SDF1 m RNA were detected by reverse transcription polymerase chain reaction(RT-PCR).Result The positive expression rates of CXCR4 protein and relative expression of CXCR4 mRNA in gastric cancer tissues and adjacent tissues were significantly higher than that in normal gastric tissues(P<0.05).There was no significant difference in the positive expression rate of SDF1 protein and the relative expression level of SDF1 mRNA between normal gastric tissue,adjacent tissue and gastric cancer tissue(P>0.05).While clinical staging and lymph node metastasis were associated with the positive expression of CXCR4 protein and the relative expression of CXCR4 mRNA,with statistically significant differences observed(P<0.05).Conclusion The expression of CXCR4 protein and CXCR4 mRNA in gastric cancer tissues are increased significantly,which may be related to the clinical stage and lymph node metastasis.
引文
[1]Stollberg S,Kaemmerer D,Neubauer E,et al.Erratum to:differential somatostatin and CXCR4 chemokine receptor expression in MALT-type lymphoma of gastric and extragastric origin[J].J Cancer Res Clin Oncol,2017,143(1):187.
[2]Cheng Y,Song Y,Qu J,et al.The chemokine receptor CXCR4and c-MET cooperatively promote epithelial-mesenchymal transition in gastric cancer cells[J].Transl Oncol,2018,11(2):487-497.
[3]刘国栋,李晓波,李昌荣,等.早期胃癌淋巴结转移的研究进展[J].中华消化外科杂志,2016,15(1):93-96.
[4]闵丛丛,张静,丁士刚.胃癌淋巴结转移分子标志物的研究进展[J].中国微创外科杂志,2018,28(1):68-71.
[5]Lin X,Xu Q,Tang L,et al.Regorafenib inhibited gastric cancer cells growth and invasion via CXCR4 activated Wnt pathway[J].PLoS One,2017,12(5):e0177335.
[6]王佳,王泽华.抑制CXCR4基因调控Wnt/β-catenin通路在卵巢癌转移中的作用[J].西安交通大学学报(医学版),2016,50(5):658-662.
[7]康运凯,吴学炜,史小芹,等.七项血清标志物在胃癌患者术前术后检测的临床意义[J].中华检验医学杂志,2017,40(1):60-63.
[8]李冬冬,陈磊,胡春华,等.血清肿瘤标志物联合检测在胃癌腹膜转移诊断中的应用价值分析[J].中国普通外科杂志,2017,26(10):1291-1296.
[9]Sakitani K,Hayakawa Y,Deng H,et al.CXCR4-expressing Mist1+progenitors in the gastric antrum contribute to gastric cancer development[J].Oncotarget,2017,8(67):111012-111025.
[10]孙昌裕,邓靖宇,梁寒.早期胃癌体液中分子标志物研究进展[J].中国肿瘤临床,2016,43(13):577-580.
[11]辛琪,张勤,张娜,等.趋化因子配体12、趋化因子受体7在肠型胃癌中的表达与淋巴结和肝脏转移的关系[J].中华消化杂志,2016,36(11):740-745.
[12]Xue LJ,Mao XB,Ren LL,et al.Inhibition of CXCL12/CXCR4 axis as a potential targeted therapy of advanced gastric carcinoma[J].Cancer Med,2017,6(6):1424-1436.
[13]Xiang Z,Zhou ZJ,Xia GK,et al.A positive crosstalk between CXCR4 and CXCR2 promotes gastric cancer metastasis[J].Oncogene,2017,36(36):5122-5133.
[14]赵艳,金鑫,李楠,等.ALDH1、CXCR4、E-cadherin在胃癌组织中的表达及与淋巴结转移的关系[J].南方医科大学学报,2016,36(10):1390-1395.
[15]周仁森,程卓鑫,刘伟新.CXCL12/CXCR4在早期胃癌中的表达及其作用[J].中国保健营养,2017,27(34):122-123.
[2]Cheng Y,Song Y,Qu J,et al.The chemokine receptor CXCR4and c-MET cooperatively promote epithelial-mesenchymal transition in gastric cancer cells[J].Transl Oncol,2018,11(2):487-497.
[3]刘国栋,李晓波,李昌荣,等.早期胃癌淋巴结转移的研究进展[J].中华消化外科杂志,2016,15(1):93-96.
[4]闵丛丛,张静,丁士刚.胃癌淋巴结转移分子标志物的研究进展[J].中国微创外科杂志,2018,28(1):68-71.
[5]Lin X,Xu Q,Tang L,et al.Regorafenib inhibited gastric cancer cells growth and invasion via CXCR4 activated Wnt pathway[J].PLoS One,2017,12(5):e0177335.
[6]王佳,王泽华.抑制CXCR4基因调控Wnt/β-catenin通路在卵巢癌转移中的作用[J].西安交通大学学报(医学版),2016,50(5):658-662.
[7]康运凯,吴学炜,史小芹,等.七项血清标志物在胃癌患者术前术后检测的临床意义[J].中华检验医学杂志,2017,40(1):60-63.
[8]李冬冬,陈磊,胡春华,等.血清肿瘤标志物联合检测在胃癌腹膜转移诊断中的应用价值分析[J].中国普通外科杂志,2017,26(10):1291-1296.
[9]Sakitani K,Hayakawa Y,Deng H,et al.CXCR4-expressing Mist1+progenitors in the gastric antrum contribute to gastric cancer development[J].Oncotarget,2017,8(67):111012-111025.
[10]孙昌裕,邓靖宇,梁寒.早期胃癌体液中分子标志物研究进展[J].中国肿瘤临床,2016,43(13):577-580.
[11]辛琪,张勤,张娜,等.趋化因子配体12、趋化因子受体7在肠型胃癌中的表达与淋巴结和肝脏转移的关系[J].中华消化杂志,2016,36(11):740-745.
[12]Xue LJ,Mao XB,Ren LL,et al.Inhibition of CXCL12/CXCR4 axis as a potential targeted therapy of advanced gastric carcinoma[J].Cancer Med,2017,6(6):1424-1436.
[13]Xiang Z,Zhou ZJ,Xia GK,et al.A positive crosstalk between CXCR4 and CXCR2 promotes gastric cancer metastasis[J].Oncogene,2017,36(36):5122-5133.
[14]赵艳,金鑫,李楠,等.ALDH1、CXCR4、E-cadherin在胃癌组织中的表达及与淋巴结转移的关系[J].南方医科大学学报,2016,36(10):1390-1395.
[15]周仁森,程卓鑫,刘伟新.CXCL12/CXCR4在早期胃癌中的表达及其作用[J].中国保健营养,2017,27(34):122-123.