黄芩苷对副猪嗜血杆菌诱导后猪血管内皮细胞黏附分子表达的影响
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摘要
为了探讨黄芩苷对副猪嗜血杆菌(HPS)诱导后猪血管内皮细胞黏附分子E-选择素(E-selectin)、细胞间黏附分子1(ICAM-1)和血管细胞黏附分子(VCAM-1)的影响,体外培养猪血管内皮细胞,接种副猪嗜血杆菌。用ELISA法检测细胞培养液中E-selectin、ICAM-1和VCAM-1释放量;用Q-RT PCR法检测血管内皮细胞内E-selectin、ICAM-1和VCAM-1的mRNA表达水平。结果表明:HPS感染猪血管内皮细胞后,细胞培养液中E-selectin、ICAM-1、VCAM-1的释放量和细胞中E-selectin、ICAM-1、VCAM-1的mRNA表达水平都极显著地上调(P <0. 01);加入药物干预后,100μg/mL的黄芩苷能极显著地抑制细胞培养液中E-selectin、ICAM-1、VCAM-1的释放量和细胞中E-selectin、ICAM-1、VCAM-1的mRNA表达水平(P <0. 01)。实验表明HPS感染猪血管内皮细胞后可以激活细胞黏附分子,黄芩苷可以抑制细胞黏附分子的表达。
In order to investigate the effect of baicalin on the expression of vascular endothelial cell adhesion molecules( E-selectin,ICAM-1,VCAM-1) induced by Haemophilus parasuis( HPS),porcine vascular endothelial cells were co-cultured with Haemophilus parasuis. The amount of E-selectin,ICAM-1 and VCAM-1 in cell culture medium was detected by ELISA. The mRNA expression levels of E-selectin,ICAM-1 and VCAM-1 in vascular endothelial cells were detected by Q-RT PCR. The results showed that after HPS infection with porcine vascular endothelial cells,the release of E-selectin,ICAM-1,VCAM-1 and the expression of E-selectin,ICAM-1 and VCAM-1 at mRNA levels were significantly up-regulated( P < 0. 01); After adding drugs,baicalin at 100 μg/m L can significantly inhibit the release of E-selectin,ICAM-1,VCAM-1 in cell culture and E-selectin,ICAM-1,VCAM-1 mRNA expression levelsin vascular endothelial cells( P < 0. 01). Our results showed that HPS could induce the expression of cell adhesion molecules and baicalin could inhibit the expression of cell adhesion molecules.
In order to investigate the effect of baicalin on the expression of vascular endothelial cell adhesion molecules( E-selectin,ICAM-1,VCAM-1) induced by Haemophilus parasuis( HPS),porcine vascular endothelial cells were co-cultured with Haemophilus parasuis. The amount of E-selectin,ICAM-1 and VCAM-1 in cell culture medium was detected by ELISA. The mRNA expression levels of E-selectin,ICAM-1 and VCAM-1 in vascular endothelial cells were detected by Q-RT PCR. The results showed that after HPS infection with porcine vascular endothelial cells,the release of E-selectin,ICAM-1,VCAM-1 and the expression of E-selectin,ICAM-1 and VCAM-1 at mRNA levels were significantly up-regulated( P < 0. 01); After adding drugs,baicalin at 100 μg/m L can significantly inhibit the release of E-selectin,ICAM-1,VCAM-1 in cell culture and E-selectin,ICAM-1,VCAM-1 mRNA expression levelsin vascular endothelial cells( P < 0. 01). Our results showed that HPS could induce the expression of cell adhesion molecules and baicalin could inhibit the expression of cell adhesion molecules.
引文
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[3] Wu S,Sun A,Liu R. Separation and purification of baicalin and wogonoside from the Chinese medicinal plant Scutellaria baicalensis Georgi by high-speed counter-current chromatography[J]. Journal of Chromatography A,2005,1066(1):243-247.
[4] Marks F S,JoséReck Jr,Almeida L L,et al.Porcine circovirus 2(PCV2)induces a procoagulant state in naturally infected swine and in cultured endothelial cells[J]. Veterinary Microbiology,2010,141(1-2):22-30.
[5] Caterina R D,Libby P,Peng H B,et al. Nitric oxide decreases cytokine-induced endothelial activation. Nitric oxide selectively reduces endothelial expression of adhesion molecules and proinflammatory cytokines[J]. Journal of Clinical Investigation,1995,96(1):60.
[6]费保莹.细胞粘附分子与炎症性肠病[J].国外医学:消化系统病分册,1999,1:15-17.