新疆阿魏菇细菌性斑点病病原菌的鉴定
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摘要
【目的】研究新疆地区阿魏菇细菌性斑点病的病原菌。【方法】应用菌落特征、致病性测定、16S rDNA序列测定、序列同源性比较,及生理生化反应特征,对阿魏菇细菌性斑点病病原菌分离物进行鉴定。【结果】从阿魏菇子实体病害样品中分离到19个单菌落分离物,并对该19个分离物进行致病性检测,其中有8个分离物在健康阿魏菇子实体上产生了细菌性斑点病症状,通过16S rDNA序列比对、序列同源性比较和生理生化反应分析,该8个分离物均为假单胞杆菌(Pseudomonas spp.)。【结论】新疆地区阿魏菇细菌斑点病由假单胞杆菌(Pseudomonas spp.)引起。
【Objective】The objective of this study is to identify the pathogens that cause bacterial spot disease of Pleurotus ferulae in Xinjiang. 【Method】The causal agents were identified by colony characterization,pathogenicity test,16 S rDNA sequence analyses,sequence homology comparison,and biochemical characterization tests. 【Result】Nineteen bacterial isolates were isolated from diseased samples of Pleurotus ferulae. Through pathogenicity test,eight tested isolates caused typical symptoms of bacterial spot disease on the healthy fruiting bodies of Pleurotus ferulae. Based on pathogenicity test,and combined with the results of 16 S rDNA sequence analyses,sequence homology comparison,biochemical and molecular characterization,eight isolates were identified as Pseudomonas spp. 【Conclusion】The bacterial spot disease on Pleurotus ferulae in Xinjiang is incited by Pseudomonas spp.
【Objective】The objective of this study is to identify the pathogens that cause bacterial spot disease of Pleurotus ferulae in Xinjiang. 【Method】The causal agents were identified by colony characterization,pathogenicity test,16 S rDNA sequence analyses,sequence homology comparison,and biochemical characterization tests. 【Result】Nineteen bacterial isolates were isolated from diseased samples of Pleurotus ferulae. Through pathogenicity test,eight tested isolates caused typical symptoms of bacterial spot disease on the healthy fruiting bodies of Pleurotus ferulae. Based on pathogenicity test,and combined with the results of 16 S rDNA sequence analyses,sequence homology comparison,biochemical and molecular characterization,eight isolates were identified as Pseudomonas spp. 【Conclusion】The bacterial spot disease on Pleurotus ferulae in Xinjiang is incited by Pseudomonas spp.
引文
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[2]张瑞颖,左雪梅,姜瑞波,等.平菇褐斑病病原菌的分离与鉴定[J].中国食用菌,2007,26(5):58-60.ZHANG Rui-ying,ZUO Xue-mei,JIANG Rui-bo,et al.(2007).Isolation and Identification of Pathogenic Bacteria Caused Brown Blotch Disease of Oyster mushroom[J].Edible Fungi of China,26(5):58-60.(in Chinese)
[3]张瑞颖,胡丹丹,左雪梅,等.平菇和双孢菇细菌性褐斑病研究进展[J].植物保护学报,2007,34(5):549-554.ZHANG Rui-ying,HU Dan-dan,ZUO Xue-mei,et al.(2007).Research advancement on brown blotch disease of oyster mushroom and button mushroom[J].Acta Phytophylacica Sinica,34(5):549-554.(in Chinese)
[4]努尔孜亚,郝敬喆,魏鹏,等.北疆地区食用菌细菌性褐斑病诊断与防治药剂筛选[J].新疆农业科学,2012,49(12):2234-2 238.Nuerziya,HAO Jing-zhe,WEI Peng,et al.(2012).Occurrence and Identification of Bacterial Brown Patch of Processing Mushrooms in Nortnern Xinjiang[J].Xinjiang Agricultural Sciences,49(12):2,234-2238.(in Chinese)
[5]金丹,李宝聚,石延霞,等.一种平菇褐斑病病原菌的鉴定[J].食用菌学报,2009,16(1):89-91.JIN Dan,LI Bao-ju,SHI Yan-xia,et al.(2009).Identification of a pathogenic bacteria caused brown blotch disease of oyster mushroom[J].Acta Edulis Fungi,16(1):89-91.(in Chinese)
[6]Yun,Y.B.,Park,S.W.,Cha,J.S.,&Kim,Y.K.(2013).Biological characterization of various strains of pseudomonas tolaasii,that causes brown blotch disease.Applied Biological Chemistry,56(1):41-45.
[7]东秀珠,蔡妙英.常见细菌系统鉴定手册[K].北京:科学出版社,2001:353-398.DONG Xiu-zhu,CAI Miao-ying.(2001).Handbook of common bacterial system identification[K].Beijing:Science Press:353-398.(in Chinese)
[8]R.E.布坎南,N.E.吉本斯,等.伯杰细菌鉴定手册(第八版)[K].北京:科学出版社,1984:128-171.R.E.Buchanan,N.E.Gibbons,et al.(1984).Bergey's manual of systematic bacteriology[K].Beijing:Science Press:128-171.(in Chinese)
[9]王晶.食用菌病害的调查与新病害病原菌鉴定研究[D].沈阳:沈阳农业大学硕士论文,2010.WANG Jing.(2010).The survey of the mushroom diseases and the identification of new occurring diseases pathogens[D].Master Dissertation.Shenyang Agricultural University,Shenyang.(in Chinese)
[10]郭亚辉,郭坚华,李斌.根据16S rRNA序列对假单胞菌属分类学的研究进展[J].微生物学杂志,2004,24(2):38-41.GUO Ya-hui,GUO Jian-hua,LI Bin.(2004).Advance of Taxon of Pseudomonas Based on 16S rRNA Sequence[J].Journal of Microbiology,24(2):38-41.(in Chinese)