珠芽魔芋对细菌性软腐病的抗性鉴定研究
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摘要
采用魔芋Amorphophallus spp.块茎点种、注射、灌根接种及田间调查等方法,对国内普遍栽种的珠芽红魔芋A.bulbifer、珠芽金魔芋A.muelleri、花魔芋A.konjac和白魔芋A.albus等12个种质材料进行抗软腐病鉴定、比较和评价,以分析珠芽魔芋对抗细菌性软腐病的抗病水平。结果表明,供试材料对软腐病抗性差异较大,珠芽金魔芋种质对细菌性软腐病均有免疫性(I),德宏及临沧珠芽红魔芋种质为高抗病品种(HR);缅甸珠芽红魔芋为抗病品种(R);富源花魔芋、楚雄花魔芋、日本农林2号、鄂魔芋1号、秦魔1号、昭通白魔芋、丽江白魔芋均属易感品种(S),与田间抗性调查情况基本相符。
Soft rot disease of Erwinia carotovora subsp. carotovora in konjac is the most important diseases. The resistence to soft rot disease of Erwinia carotovora subsp. carotovora among 12 konjac(Amorphophallus spp.) cultivars were evaluated by different inoculation methods and fields disease survey. The results showed that A. konjac and A. albus were susceptible cultivars(S), A. bulbifer from Dehong city and Lincang city were highly resistant materials(HR), A. bulbifer from Myanmar was resistant materials(R), while A. muelleri was immune resistant(I). The results confirmed to fields disease survey.
Soft rot disease of Erwinia carotovora subsp. carotovora in konjac is the most important diseases. The resistence to soft rot disease of Erwinia carotovora subsp. carotovora among 12 konjac(Amorphophallus spp.) cultivars were evaluated by different inoculation methods and fields disease survey. The results showed that A. konjac and A. albus were susceptible cultivars(S), A. bulbifer from Dehong city and Lincang city were highly resistant materials(HR), A. bulbifer from Myanmar was resistant materials(R), while A. muelleri was immune resistant(I). The results confirmed to fields disease survey.
引文
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[18]邓利玲,徐小青,杨延迅,王俏,钟耕.珠芽魔芋精粉的理化性质[J].食品科学,2013,34(17):120—125.
[19]黄晴.两种魔芋精粉的基础应用特性研究[D].武汉:华中农业大学硕士学位论文,2016.
[20]雷珍珍,叶晶龙,程海丽,陈云,望彗星,许克静,乐超银.花魔芋抗软腐病植株的鉴定及其抗性机理的初步研究[J].植物学报,2013,48(3):295—302.
[21]王慧荣,万剑吟,万剑真.魔芋新品种—珠芽魔芋的初步应用研究[J].农产品加工(学刊),2012(5):130—134.
[22]赵家君,陈雁,欧辉.魔芋软腐病田间药剂防治研究[J].耕作与栽培,2002(2):52—53.
[23]刘晓妹,刘文波,蒲金基,谢艺贤,张欣,漆艳香,李少华.杧果对细菌性黑斑病抗病性测定[J].果树学报,2009,26(3):349—352.
[24]Pan C,Gichira A W,Chen J M.Genetic variation in wild populations of the tuber crop Amorphophallus konjac(Araceae)in central China as revealed by AFLP markers[J].Genetics&Molecular Research,2015,14(4):18753.
[25]陈国爱,郭邦利,刘婷,郑敏,段龙飞.魔芋新品种选育研究进展[J].长江蔬菜,2015(4):5—7.
[26]寸湘琴,赵庆云,黎亚东,谢世清.云南高原特有魔芋种质的性状分析[J].中国农学通报,2005(6):207—212.
[27]Pravi V,Jeeva M L,Archana P V.Nucleic acid spot hybridization based species-specific detection of Sclerotium rolfsii associated with collar rot disease of Amorphophallus paeoniifolius[J].World Journal of Microbiology&Biotechnology,2015,31(2):315—320.
[2]Impaprasert R,Srzednicki G,Borompichaichartkul C,Zhao J R,Yu L.Improving production of purified konjac glucomannan from Amorphophallus muelleri by multistage drying[J].Acta Horticulturae,2013,1011(1011):155—162.
[3]Zhao J R.Integrated production of purified konjac flour[D].Bangkok:KMUTT,2011.
[4]Yu L,Zhao J R,Xu S G,Su Y,Gao D,Srzednicki G.First report of gray mold on Amorphophallus muelleri caused by Botrytis cinerea in China[J].Plant Disease,2014,98(5):692.
[5]Zhao J R,Yu L,Srzednicki G,Borompichaichartkul C.Effects of different concentrations of gibberellin on flower-bud differentiation of Amorphophallus muelleri[C]//Pattaya,Thailand:Proceedings of International Symposium on Agri-Foods for Health and Wealth,2013:93—99.
[6]姬广海,吴亚鹏,白学慧,卢俊,董坤.抗生素溶杆菌对魔芋软腐病和根际微生物多样性的影响[J].江西农业大学学报,2009,31(3):499—503.
[7]叶晶龙,乐超银,潘虹,廖甜甜,李金鞠,刘海军,王钧.魔芋软腐病拮抗放线菌的筛选及其抑菌活性研究[J].安徽农业科学,2010,38(30):16908—16910.
[8]张红骥,邵梅,杜鹏,卢俊,何霞红,于德才,朱有勇.云南省魔芋与玉米多样性栽培控制魔芋软腐病[J].生态学杂志,2012,31(2):332—336.
[9]崔鸣,李川.魔芋软腐病的发生规律及防治技术研究进展[J].中国植保导刊,2009,29(6):33—35.
[10]刘佩英.魔芋学[M].北京:中国农业出版社,2004.
[11]崔鸣,谢利华,张盛林,王玉波,赵兴喜.不同魔芋品种产量和抗病性试验研究[J].种子,2005(12):84—85.
[12]钟伏付,苏娜,杨廷宪,孙正祥,周燚.魔芋品种选育与改良研究进展[J].湖北农业科学,2011,50(3):446—449.
[13]赵建荣,余磊.一种珠芽类魔芋快繁制种的方法:中国,CN201310061538.9[P].2013-05-15.
[14]赵建荣,余磊.一种金魔芋实生籽种多苗同体接力生长的方法:中国,CN103125238A[P].2013-06-05.
[15]闫鹏,闫建洲,李筠.秦巴山区珠芽魔芋种芋繁育方法比较及示范[J].长江蔬菜,2016(6):38—42.
[16]Zhao J R,Zhang D H,Zhao J P,Srzednicki G,Borompichaichartkul C,Kanlayanarat S.The morphological and growth characteristics of Amorphophallus muelleri blume a commercially important konjac species[J].Acta Horticulturae,2010,875(875):501—508.
[17]Zhao J R,Zhang D H,Srzednicki G,Kanlayanarat S,Borompichaichartkul C.Development of a low-cost and two-stage technique for production of low sulphur purified konjac flour[J].International Food Research Journal,2010,17(4):1113—1124.
[18]邓利玲,徐小青,杨延迅,王俏,钟耕.珠芽魔芋精粉的理化性质[J].食品科学,2013,34(17):120—125.
[19]黄晴.两种魔芋精粉的基础应用特性研究[D].武汉:华中农业大学硕士学位论文,2016.
[20]雷珍珍,叶晶龙,程海丽,陈云,望彗星,许克静,乐超银.花魔芋抗软腐病植株的鉴定及其抗性机理的初步研究[J].植物学报,2013,48(3):295—302.
[21]王慧荣,万剑吟,万剑真.魔芋新品种—珠芽魔芋的初步应用研究[J].农产品加工(学刊),2012(5):130—134.
[22]赵家君,陈雁,欧辉.魔芋软腐病田间药剂防治研究[J].耕作与栽培,2002(2):52—53.
[23]刘晓妹,刘文波,蒲金基,谢艺贤,张欣,漆艳香,李少华.杧果对细菌性黑斑病抗病性测定[J].果树学报,2009,26(3):349—352.
[24]Pan C,Gichira A W,Chen J M.Genetic variation in wild populations of the tuber crop Amorphophallus konjac(Araceae)in central China as revealed by AFLP markers[J].Genetics&Molecular Research,2015,14(4):18753.
[25]陈国爱,郭邦利,刘婷,郑敏,段龙飞.魔芋新品种选育研究进展[J].长江蔬菜,2015(4):5—7.
[26]寸湘琴,赵庆云,黎亚东,谢世清.云南高原特有魔芋种质的性状分析[J].中国农学通报,2005(6):207—212.
[27]Pravi V,Jeeva M L,Archana P V.Nucleic acid spot hybridization based species-specific detection of Sclerotium rolfsii associated with collar rot disease of Amorphophallus paeoniifolius[J].World Journal of Microbiology&Biotechnology,2015,31(2):315—320.