龙骨对抗软骨细胞氧化损伤和坐骨神经损伤的研究
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摘要
龙骨,临床用于治疗风湿性关节炎、跌打损伤、胃痛病等。本文主要研究龙骨对骨关节炎软骨细胞的氧化损伤可逆作用和促进受损伤神经组织功能修复方面的作用。采用低、中、高三组剂量,分别检测细胞内活性氧水平和细胞培养上清中NO、SOD和GSH-Px三种物质的含量。结果表明,NaNO_2和SOD测定的三组数据与模型组对比均有统计学差异。GSH-Px测定的中、高剂量组与模型组相比较有统计学差异。实验结果说明龙骨对骨关节炎软骨细胞的氧化损伤有可逆作用,对龙骨的成分含量有一定的要求范围。测定龙骨抗坐骨神经损伤,说明龙骨具有促进受损伤神经组织功能修复的作用。
Fossil fragments are used in the treatment of rheumatoid arthritis,traumatic injury,stomachache and so on. This paper mainly discusses the effects of fossil fragments on the reversibility of chondrocytes of osteoarthritis and the function of the repair of injured nerve tissue. The content of the three substances of NO,SOD and GSH-Px in the cell culture are detected by the low,medium and high doses. The results show that there are statistical differences between the data of the three groups detected by NaNO_2 and SOD and the model group,and there are statistical differences between the groups detected by medium and high doses with GSH-Px measurements and the model group,indicating that fossil fragments have a reversible effect on the treatment of oxidative injury of chondrocytes of osteoarthritis but has certain requirements for the range of contents. And the detection on the treatment of sciatic nerve injury shows that fossil fragments have a function in the repair of injured nerve tissue.
Fossil fragments are used in the treatment of rheumatoid arthritis,traumatic injury,stomachache and so on. This paper mainly discusses the effects of fossil fragments on the reversibility of chondrocytes of osteoarthritis and the function of the repair of injured nerve tissue. The content of the three substances of NO,SOD and GSH-Px in the cell culture are detected by the low,medium and high doses. The results show that there are statistical differences between the data of the three groups detected by NaNO_2 and SOD and the model group,and there are statistical differences between the groups detected by medium and high doses with GSH-Px measurements and the model group,indicating that fossil fragments have a reversible effect on the treatment of oxidative injury of chondrocytes of osteoarthritis but has certain requirements for the range of contents. And the detection on the treatment of sciatic nerve injury shows that fossil fragments have a function in the repair of injured nerve tissue.
引文
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[2]Meng X Q,Hui R H,Jiang H,et al.Effect of dissipate phlegm and dehygrosis on cytokine IL-1,IL-6,TNF-αin knee osteoarthritis in rabbits[J].Zhongguo Gu Shang/China J Orthop Trauma,2007,20(8):575.
[3]张建环,朱群娣,钟建明.我院非甾体抗炎药在治疗膝骨关节炎中的临床应用分析[J].广东药学院学报,2014,30(4):486.
[4]侯德才.膝关节骨性关节炎的分期治疗[J].中医正骨,2014,26(1):3.
[5]徐淑云.药理实验方法学[M].3版.北京:人民卫生出版社,2001:891.
[6]曾卫,向阳,潘航,等.NO与骨关节炎的关系研究进展[J].生物技术世界,2015(12):88.
[7]彭力平,马笃军,林栋栋,等.牛膝醇提物体内诱导兔骨关节炎模型软骨修复的病理学观察[J].湖南中医杂志,2013,29(2):126.
[8]王栋栋,熊健斌.兔膝骨关节炎动物模型的构建[J].吉林医学,2013,34(33):6883.
[9]宋昕恬,张晶莹,孟令仪,等.人参三醇组皂苷对大鼠坐骨神经急性损伤后修复的研究[J].中国卫生工程学,2016,15(4):318.
[10]Zhang GZ,Liu J,Zhou YB,et al.Panax ginseng ginsenoside-Rg(2)protects memory impairment via antiapoptosis in a rat model with vascular dementia[J].J Ethnopharmacol,2008,115(3):441.