口咽部正常菌群对肺炎支原体快速培养法试剂盒的影响
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摘要
目的观察常见的口咽部正常菌群对肺炎支原体快速培养法试剂盒结果的影响,评价其临床实用性和可靠性。方法用标准菌株和口咽部分离的临床菌株作为研究对象,观察不同细菌对培养结果的影响。结果标准菌株肺炎链球菌、金黄色葡萄球菌、流感嗜血杆菌、大肠埃希菌、白念珠菌在试剂盒中的培养阳性率分别为0,10.00%,10.00%,10.00%,20.00%,临床分离的野生型和耐药型草绿色链球菌培养阳性率分别为5.00%,80.00%;表皮葡萄球菌为15.00%,90.00%;肺炎克雷伯菌为25.00%,80.00%,白念珠菌为20.00%,85.00%;鲍曼不动杆菌为90.00%,100.00%。除鲍曼不动杆菌外其它四种耐药型菌株的培养阳性率明显增高。结论口咽部若存在耐药菌或多重耐药菌以及对某些抗生素有天然耐药机制的细菌时会造成肺炎支原体快速培养法检测结果出现假阳性,在试验结果报告中应注意其假阳性的问题。
Objective To observe the effect of common oropharyngeal normal flora on the results of the rapid culture method of Mycoplasma pneumoniae and evaluate its clinical applicability and reliability.Methods The clinical strains isolated from standard strains and oropharynx were used as research objects to observe the effects of different bacteria on the culture results. Results The positive rates of standard strains of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Escherichia coli and Candida albicans in the kit were 0, 10.00%, 10.00%,10.00%, 20.00%, clinical The positive rates of isolated wild-type and drug-resistant S. cerevisiae cultures were 5.00% and 80.00%, respectively;Staphylococcus epidermidis was 15.00%, 90.00%; Klebsiella pneumoniae was 25.00%, 80.00%, and Candida albicans was 20.00%,85.00%;Acinetobacter baumannii was 90.00%, 100.00%. The positive rate of culture of the four drug-resistant strains except Acinetobacter baumannii was significantly increased.Conclusion If there are resistant or multi-drug resistant bacteria in the oropharynx and bacteria with natural resistance to certain antibiotics, the results of the rapid culture method of mycoplasma pneumoniae will be false positive, and the problem of false positives should be noted in the test results report.
Objective To observe the effect of common oropharyngeal normal flora on the results of the rapid culture method of Mycoplasma pneumoniae and evaluate its clinical applicability and reliability.Methods The clinical strains isolated from standard strains and oropharynx were used as research objects to observe the effects of different bacteria on the culture results. Results The positive rates of standard strains of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Escherichia coli and Candida albicans in the kit were 0, 10.00%, 10.00%,10.00%, 20.00%, clinical The positive rates of isolated wild-type and drug-resistant S. cerevisiae cultures were 5.00% and 80.00%, respectively;Staphylococcus epidermidis was 15.00%, 90.00%; Klebsiella pneumoniae was 25.00%, 80.00%, and Candida albicans was 20.00%,85.00%;Acinetobacter baumannii was 90.00%, 100.00%. The positive rate of culture of the four drug-resistant strains except Acinetobacter baumannii was significantly increased.Conclusion If there are resistant or multi-drug resistant bacteria in the oropharynx and bacteria with natural resistance to certain antibiotics, the results of the rapid culture method of mycoplasma pneumoniae will be false positive, and the problem of false positives should be noted in the test results report.
引文
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[7]郭义兵.影响肺炎支原体液体培养假阳性的因素分析[J].医药前沿,2016,6(13):393-394.
[8]李昌庆,李岑,熊燕,等.肺炎支原体液体培养法的实验诊断学评价[J].四川医学,2016,37(12):1362-1366.
[2]Katsushima Y,Katsushima F,Suzuki Y,et al.Characteristics of Mycoplasma pneumoniae infection identified on culture in a pediatric clinic[J].Pediatr Int,2015,57(2):247-252.
[3]Zhao F,Liu L,Tao X,et al.Culture-Independent Detection and Genotyping of Mycoplasma pneumoniae in Clinical Specimens from Beijing,China[J].PLoS One,2015,10(10):e0141702.
[4]Ma YJ,Wang SM,Cho YH,et al.Clinical and epidemiological characteristics in children with community-acquired mycoplasma pneumonia in Taiwan:A nationwide surveillance[J].J Microbiol Immunol Infect,2015,48(6):632-638.
[5]路畅,马艺铭.快速血清学检验和微生物快速培养检测诊断小儿肺炎支原体感染的价值分析[J].临床检验杂志(电子版),2018,7(3):382-383
[6]赵汉青,闫超,孙红妹,等.肺炎支原体快速液体培养法的评价和存在的问题[J].中华微生物学和免疫学杂志,2016,36(7):517-518.
[7]郭义兵.影响肺炎支原体液体培养假阳性的因素分析[J].医药前沿,2016,6(13):393-394.
[8]李昌庆,李岑,熊燕,等.肺炎支原体液体培养法的实验诊断学评价[J].四川医学,2016,37(12):1362-1366.