胎盘绒毛组织代谢足迹在早发型与晚发型重度子痫前期患者中的差异
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摘要
目的对比研究早发型重度子痫前期患者和晚发型重度子痫前期患者胎盘绒毛组织在体外6%氧浓度下培养,其培养液中代谢足迹的差异。方法本研究纳入2014年1月至2016年12月就诊于我院重度子痫前期患者44例,根据子痫类型分为早发型重度子痫前期组(早发组21例)和晚发型重度子痫前期组(晚发组23例),全部患者接受剖宫产终止妊娠治疗。于胎盘母体面取绒毛组织,在6%氧浓度下培养96h,采用高效液相质谱色谱仪检测培养液中代谢产物。对比两组患者谷氨酸、谷氨酰胺、色氨酸和犬尿氨酸的浓度差异。结果在6%氧浓度下培养的早发组和晚发组绒毛组织中,36种代谢产物出现显著性差异(P<0.05);早发型重度子痫前期患者的胎盘绒毛组织培养基中,犬尿氨酸的浓度显著高于晚发型重度子痫前期组(P<0.05)。结论通过组织代谢足迹分析技术,犬尿氨酸含量可以有效鉴别早发型和晚发型重度子痫前期,对预测重度子痫前期提供新的技术途径,值得临床推广应用。
Objective:To compare the difference of the placental villus tissue of early onset severe pre eclampsia and late onset severe pre eclampsia in vitro under 6% oxygen concentration.Methods:This study included 44 cases of severe preeclampsia in our hospital from January 2014 to December 2016,according to the type of eclampsia,divided into early onset of severe preeclampsia group(early onset group,21 cases)and late onset severe preeclampsia group(late onset group,23 cases),all patients received cesarean section treatment.The villus tissue was taken from the placenta surface,and 96 h was cultured at 6% oxygen concentration.The metabolites in the culture medium were detected by hplc.The concentrations of glutamic acid,glutamine,tryptophan,and uric acid in the two groups were compared.Results:Cultured in 6% oxygen concentration of early onset and late onset groups in villi,there were significant differences between the 36 metabolites(P<0.05);Placental villus tissue culture in patients with early onset severe preeclampsia,the concentration of serum uric acid was significantly higher than that in the group of late onset severe preeclampsia(P<0.05).Conclusion:Through metabolic footprint analysis techniques,kynurenine content can effectively identify early and late onset severe preeclampsia,prediction of severe preeclampsia providing a new approach,it is worthy of clinical application.
Objective:To compare the difference of the placental villus tissue of early onset severe pre eclampsia and late onset severe pre eclampsia in vitro under 6% oxygen concentration.Methods:This study included 44 cases of severe preeclampsia in our hospital from January 2014 to December 2016,according to the type of eclampsia,divided into early onset of severe preeclampsia group(early onset group,21 cases)and late onset severe preeclampsia group(late onset group,23 cases),all patients received cesarean section treatment.The villus tissue was taken from the placenta surface,and 96 h was cultured at 6% oxygen concentration.The metabolites in the culture medium were detected by hplc.The concentrations of glutamic acid,glutamine,tryptophan,and uric acid in the two groups were compared.Results:Cultured in 6% oxygen concentration of early onset and late onset groups in villi,there were significant differences between the 36 metabolites(P<0.05);Placental villus tissue culture in patients with early onset severe preeclampsia,the concentration of serum uric acid was significantly higher than that in the group of late onset severe preeclampsia(P<0.05).Conclusion:Through metabolic footprint analysis techniques,kynurenine content can effectively identify early and late onset severe preeclampsia,prediction of severe preeclampsia providing a new approach,it is worthy of clinical application.
引文
[1]刘大艳,陈士岭,王晨虹,等.缺氧对重度子痫前期患者胎盘绒毛组织代谢足迹变化的影响[J].实用妇产科杂志,2012,28(1):40-44.DOI:10.3969/j.issn.1003-6946.2012.01.016.
[2]刘大艳,陈士岭,王晨虹,等.早发型与晚发型重度子痫前期患者胎盘绒毛组织代谢足迹差异的研究[J].南方医科大学学报,2011,9:1547-1550.
[3]刘大艳,等.低氧及常氧条件下体外培养绒毛组织代谢足迹的变化[J].中国妇幼保健,2011,26(25):3949-3952.
[4]Horgan RP,Broadhurst DI,Dunn WB,et al.Changes in the metabolic footprint of placental explant-conditionedmedium cultured in different oxygen tensions from placentas of small forgestational age and normal pregnancies[J].Placenta,2010,31(10):893-901.doi:10.1016/j.placenta.2010.07.002.
[5]宋娇,王永红.XIAP在子痫前期患者胎盘血管内皮细胞中的表达及意义[J].中华临床医师杂志(电子版),2015,(9):1583-1587.DOI:10.3877/cma.j.issn.1674-0785.2015.09.022.
[6]王莉,吴青青,马玉庆,等.妊娠晚期胎盘绒毛膜下血肿的超声与临床分析[J].中国医刊,2014,(7):81-83.DOI:10.3969/j.issn.1008-1070.2014.07.028.
[7]孙丽洲,姜子燕,任怀彬,等.胎盘绒毛组织内质网蛋白44表达与子痫前期的关系[J].国际妇产科学杂志,2011,38(3):211-213,封3.DOI:10.3969/j.issn.1674-1870.2011.03.013.
[8]Dunn WB,et al.Changes in the metabolic footprint of placentalexplant-conditioned culture medium identifies metabolic disturbances related tohypoxia and pre-eclampsia[J].Placenta,2009,30(11):974-80.doi:10.1016/j.placenta.2009.08.008.
[9]庞战军,邢福祺,苏桂栋,等.TGF-β和IGF表达异常与滋养层相关疾病关系的研究[J].现代妇产科进展,2003,12(2):89-92.DOI:10.3969/j.issn.1004-7379.2003.02.003.
[10]张晓波,贾红梅,汪颖南,等.早发型与晚发型重度子痫前期178例胎盘临床病理学观察[J].中华病理学杂志,2015,44(12):879-883.DOI:10.3760/cma.j.issn.0529-5807.2015.12.011.
[11]宋杰东,孟茜,王文荣,等.重度子痫前期患者胎盘床血流动力学的检测及意义[J].广东医学,2014,(6):862-863,864.
[12]张晓波,贾红梅,汪颖南,等.晚发型子痫前期胎盘124例临床病理分析[J].诊断病理学杂志,2016,23(12):912-915.DOI:10.3969/j.issn.1007-8096.2016.12.005.
[13]江少青,邵汝标.重度子痫前期患者胎盘床血流动力学参数的变化及临床意义[J].海南医学,2015,(22):3375-3376.DOI:10.3969/j.issn.1003-6350.2015.22.1224.
[14]刘大艳.缺氧对重度子痫前期患者胎盘绒毛组织代谢足迹变化的影响[D].南方医科大学,2011.DOI:10.7666/d.y1997425.
[15]马艳娟,刘陶,王慧,等.HLA-G在子痫前期患者血清及胎盘中的表达及意义[J].实用妇产科杂志,2014,30(6):452-456.
[2]刘大艳,陈士岭,王晨虹,等.早发型与晚发型重度子痫前期患者胎盘绒毛组织代谢足迹差异的研究[J].南方医科大学学报,2011,9:1547-1550.
[3]刘大艳,等.低氧及常氧条件下体外培养绒毛组织代谢足迹的变化[J].中国妇幼保健,2011,26(25):3949-3952.
[4]Horgan RP,Broadhurst DI,Dunn WB,et al.Changes in the metabolic footprint of placental explant-conditionedmedium cultured in different oxygen tensions from placentas of small forgestational age and normal pregnancies[J].Placenta,2010,31(10):893-901.doi:10.1016/j.placenta.2010.07.002.
[5]宋娇,王永红.XIAP在子痫前期患者胎盘血管内皮细胞中的表达及意义[J].中华临床医师杂志(电子版),2015,(9):1583-1587.DOI:10.3877/cma.j.issn.1674-0785.2015.09.022.
[6]王莉,吴青青,马玉庆,等.妊娠晚期胎盘绒毛膜下血肿的超声与临床分析[J].中国医刊,2014,(7):81-83.DOI:10.3969/j.issn.1008-1070.2014.07.028.
[7]孙丽洲,姜子燕,任怀彬,等.胎盘绒毛组织内质网蛋白44表达与子痫前期的关系[J].国际妇产科学杂志,2011,38(3):211-213,封3.DOI:10.3969/j.issn.1674-1870.2011.03.013.
[8]Dunn WB,et al.Changes in the metabolic footprint of placentalexplant-conditioned culture medium identifies metabolic disturbances related tohypoxia and pre-eclampsia[J].Placenta,2009,30(11):974-80.doi:10.1016/j.placenta.2009.08.008.
[9]庞战军,邢福祺,苏桂栋,等.TGF-β和IGF表达异常与滋养层相关疾病关系的研究[J].现代妇产科进展,2003,12(2):89-92.DOI:10.3969/j.issn.1004-7379.2003.02.003.
[10]张晓波,贾红梅,汪颖南,等.早发型与晚发型重度子痫前期178例胎盘临床病理学观察[J].中华病理学杂志,2015,44(12):879-883.DOI:10.3760/cma.j.issn.0529-5807.2015.12.011.
[11]宋杰东,孟茜,王文荣,等.重度子痫前期患者胎盘床血流动力学的检测及意义[J].广东医学,2014,(6):862-863,864.
[12]张晓波,贾红梅,汪颖南,等.晚发型子痫前期胎盘124例临床病理分析[J].诊断病理学杂志,2016,23(12):912-915.DOI:10.3969/j.issn.1007-8096.2016.12.005.
[13]江少青,邵汝标.重度子痫前期患者胎盘床血流动力学参数的变化及临床意义[J].海南医学,2015,(22):3375-3376.DOI:10.3969/j.issn.1003-6350.2015.22.1224.
[14]刘大艳.缺氧对重度子痫前期患者胎盘绒毛组织代谢足迹变化的影响[D].南方医科大学,2011.DOI:10.7666/d.y1997425.
[15]马艳娟,刘陶,王慧,等.HLA-G在子痫前期患者血清及胎盘中的表达及意义[J].实用妇产科杂志,2014,30(6):452-456.