宫颈癌合并生殖道感染HPV DNA检测及感染分析
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摘要
目的探究HPV DNA感染与宫颈癌相关的检查指标对宫颈癌的诊断价值,了解宫颈癌患者及慢性宫颈炎患者生殖道感染的病原菌分布。方法选择2015年7月—2017年5月来我院就诊的50例宫颈癌合并生殖道感染的患者作为观察组,选择同期来我院就诊的50例慢性宫颈炎的患者作为对照组。分析TCT、HPV DNA、SCCA、CA15-3、CA125、CYFRA21-1、HPV E6/E7 m RNA诊断宫颈癌的敏感性、准确性、阳性预测值和阴性预测值。并分析宫颈癌和宫颈炎患者的病原菌分布情况。结果 HPV DNA检测诊断宫颈癌的敏感性及准确性高于TCT、SCCA、CA15-3、CA125、CYFRA21-1检测及HPV E6/E7m RNA检测(P <0.05),且阳性预测值高。宫颈癌患者和宫颈炎患者生殖道感染均以革兰式阴性菌为主。结论 HPV DNA检测在宫颈癌诊断中具有较高的敏感性,是宫颈癌筛查的重要临床方法。宫颈癌组和宫颈炎组组生殖道感染均以革兰式阴性菌为主,其中宫颈癌合并生殖道感染患者阴道内以大肠埃希菌、肺炎克雷伯菌为主。
Objective To explore the diagnostic value of HPV DNA infection and cervical cancer related examination indicators for cervical cancer, and to understand the distribution of pathogens of genital tract infection in patients with cervical cancer and chronic cervicitis. Methods50 patients with cervical cancer complicated with genital tract infection who came to our hospital from July 2015 to May 2017 were selected as observation group, and 50 patients with chronic cervicitis who came to our hospital during the same period were selected as control group. The sensitivity, accuracy, positive predictive value and negative predictive value of TCT, HPV DNA, SCCA, CA15-3, CA125, CYFRA21-1 and HPV E6/E7 in the diagnosis of cervical cancer were analyzed. The distribution of pathogenic bacteria in patients with cervical cancer and cervicitis was analyzed. Results The sensitivity and accuracy of HPV DNA detection in the diagnosis of cervical cancer were significantly higher than those of TCT, SCCA, CA15-3, CA125, CYFRA21-1 and HPV E6/E7(P < 0.05), and the positive predictive value was high. Genital tract infections in cervical cancer patients and cervicitis patients were mainly gram-negative bacteria. Conclusion HPV DNA detection has high sensitivity in the diagnosis of cervical cancer and is an important clinical method for cervical cancer screening. Genital tract infections in cervical cancer group and cervicitis group were mainly gram-negative bacteria. Escherichia coli and Klebsiella pneumoniae were predominant in vagina of patients with cervical cancer and genital tract infections.
Objective To explore the diagnostic value of HPV DNA infection and cervical cancer related examination indicators for cervical cancer, and to understand the distribution of pathogens of genital tract infection in patients with cervical cancer and chronic cervicitis. Methods50 patients with cervical cancer complicated with genital tract infection who came to our hospital from July 2015 to May 2017 were selected as observation group, and 50 patients with chronic cervicitis who came to our hospital during the same period were selected as control group. The sensitivity, accuracy, positive predictive value and negative predictive value of TCT, HPV DNA, SCCA, CA15-3, CA125, CYFRA21-1 and HPV E6/E7 in the diagnosis of cervical cancer were analyzed. The distribution of pathogenic bacteria in patients with cervical cancer and cervicitis was analyzed. Results The sensitivity and accuracy of HPV DNA detection in the diagnosis of cervical cancer were significantly higher than those of TCT, SCCA, CA15-3, CA125, CYFRA21-1 and HPV E6/E7(P < 0.05), and the positive predictive value was high. Genital tract infections in cervical cancer patients and cervicitis patients were mainly gram-negative bacteria. Conclusion HPV DNA detection has high sensitivity in the diagnosis of cervical cancer and is an important clinical method for cervical cancer screening. Genital tract infections in cervical cancer group and cervicitis group were mainly gram-negative bacteria. Escherichia coli and Klebsiella pneumoniae were predominant in vagina of patients with cervical cancer and genital tract infections.
引文
[1]靳荣,李红芳.泛素水解酶22si RNA对宫颈癌CD133+宫颈癌干细胞增殖和侵袭的影响[J].中山大学学报(医学科学版),2016,37(1):48-53.
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[8]任菲,陈军莹,杜萍,丁楠,等.宫颈癌组织mi R-574-5p的表达及其下调后对宫颈癌Si Ha细胞的影响[J].基础医学与临床,2015,35(4):458-462.
[9]李霞,杨爱凤,杜娟,等.宫颈癌患者合并生殖道感染病原学特点与预防措施分析[J].中华医院感染学杂志,2017,27(15):3544-3547.
[10]李剑,曲芃芃.HPVE6/E7m RNA与HPVDNA检测在宫颈癌早期筛查中的临床价值[J].天津医药,2016,44(4):466-469.
[11]李敏言.TCT、人乳头瘤病毒DNA检查联合阴道镜检查在宫颈癌早期筛查中的价值研究[J].中国生化药物杂志,2017,37(8):369-371.
[12]李红,罗艳,晁晶,等.生殖道感染与宫颈癌及宫颈上皮内瘤变的相关性[J].中国妇幼保健,2017,32(11):2427-2428.
[2]魏倩,张鹏.mi R-17-5p在宫颈癌组织中的表达变化及其对宫颈癌细胞生长增殖影响[J].山东医药,2015,55(43):8-10.
[3]杨建敏,胡静文.Foxm1蛋白在人宫颈癌组织中的表达及与病理类型、分级、分期之间的关系[J].广东医学,2016,37(14):2150-2154.
[4]姚淑娟,伏世杰,张师前.生殖道感染与宫颈癌[J].医学综述,2018,24(10):1898-1902.
[5]洪恩.宫颈上皮内瘤变与生殖道感染关系的临床分析[J].中国医药科学,2018,8(5):227-229,232.
[6]郭秦,郭建巍.HPV检测中的自采样--让宫颈癌筛查更有效[J].医学综述,2019,25(3):464-469.
[7]韩钦,郭红燕.宫颈癌筛查中HPV检测初筛的分流检测[J].实用妇产科杂志,2016,32(8):577-580.
[8]任菲,陈军莹,杜萍,丁楠,等.宫颈癌组织mi R-574-5p的表达及其下调后对宫颈癌Si Ha细胞的影响[J].基础医学与临床,2015,35(4):458-462.
[9]李霞,杨爱凤,杜娟,等.宫颈癌患者合并生殖道感染病原学特点与预防措施分析[J].中华医院感染学杂志,2017,27(15):3544-3547.
[10]李剑,曲芃芃.HPVE6/E7m RNA与HPVDNA检测在宫颈癌早期筛查中的临床价值[J].天津医药,2016,44(4):466-469.
[11]李敏言.TCT、人乳头瘤病毒DNA检查联合阴道镜检查在宫颈癌早期筛查中的价值研究[J].中国生化药物杂志,2017,37(8):369-371.
[12]李红,罗艳,晁晶,等.生殖道感染与宫颈癌及宫颈上皮内瘤变的相关性[J].中国妇幼保健,2017,32(11):2427-2428.