Zeb1、CtBP1与E-cadherin在结肠腺癌中的表达及意义
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摘要
目的:探讨转录抑制因子Zeb1、转录辅抑制因子CtBP1在结肠腺癌中的表达及与其靶基因Ecadherin的关系。方法:采用免疫组织化学染色方法检测结肠腺癌及癌旁组织中E-cadherin、Zeb1和CtBP1表达。结果:CtBP1在结肠腺癌组织及癌旁组织中表达阳性率分别为96.25%和100%,Zeb1分别为28.75%和0%,E-cadherin分别为32.50%和100%。E-cadherin表达和CtBP1表达不相关,而与Zeb1表达成负相关。结论:结肠腺癌中Zeb1与E-cadherin均表达异常;结肠腺癌中Zeb1异常表达可能是E-cadherin表达下调的机制。
Objective:To investigate the expression of transcriptional suppressor Zeb1and CtBP1, and the relationships with their target gene E-cadherin in colon adenocarcinoma.Methods:The expression of CtBP1,Zeb1and E-cadherin proteins in colon adenocarcinoma and the paired non-neoplastic tissue were detected by the immunohistochemistry staining method.Results:The positive rates of CtBP1expression in colon adenocarcinoma and the paired nonneoplastic tissue were 96.25% and 100%.The positive rate of Zeb1expression in colon adenocarcinoma and paired non-neoplastic tissue was 28.75%and 0%respectively.The positive rate of E-cadherin expression in colon adenocarcinoma and paired non-neoplastic tissue was 32.50%and 100%.The E-cadherin expression was not related with CtBP1expression,but was negatively correlated with Zeb1in colon adenocarcinoma.Conclusion:Zeb1and E-cadherin deregulate in colon adenocarcinoma,and the abnormal expression of Zeb1,has been involoved in the down-regulation of E-cadherin mechanism.
Objective:To investigate the expression of transcriptional suppressor Zeb1and CtBP1, and the relationships with their target gene E-cadherin in colon adenocarcinoma.Methods:The expression of CtBP1,Zeb1and E-cadherin proteins in colon adenocarcinoma and the paired non-neoplastic tissue were detected by the immunohistochemistry staining method.Results:The positive rates of CtBP1expression in colon adenocarcinoma and the paired nonneoplastic tissue were 96.25% and 100%.The positive rate of Zeb1expression in colon adenocarcinoma and paired non-neoplastic tissue was 28.75%and 0%respectively.The positive rate of E-cadherin expression in colon adenocarcinoma and paired non-neoplastic tissue was 32.50%and 100%.The E-cadherin expression was not related with CtBP1expression,but was negatively correlated with Zeb1in colon adenocarcinoma.Conclusion:Zeb1and E-cadherin deregulate in colon adenocarcinoma,and the abnormal expression of Zeb1,has been involoved in the down-regulation of E-cadherin mechanism.
引文
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[9]Zhang X L,Huang C X,Zhang J,et al.CtBP1is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma[J].Oncol Rep,2013,30(2):809.
[10]Nadauld L D,Phelps R,Moore B C,et al.Adenomatous polyposis coli control of C-terminal binding protein-1stability regulates expression of intestinal retinol dehydrogenases[J].J Biol Chem,2006,281(49):37828-37835.
[2]Pe珘na C,García J M,García V,et al.The expression levels of the transcriptional regulators p300and CtBP modulate the correlations between SNAIL,ZEB1,E-cadherin and vitamin D receptor in human colon carcinomas[J].Int J Cancer,2006,119(9):2098.
[3]Postigo A A,Depp J L,Taylor J J,et al.Regulation of Smad signaling through a differential recruitment of coactivators and corepressors by ZEB proteins[J].EMBO J,2003,22(10):2453-2462.
[4]Yilmaz M,Christofori G,Lehembre F.Distinct mechanisms of tumor invasion and metastasis[J].Trends Mol Med,2007,13(12):535-541.
[5]Tsanou E,Peschos D,Batistatou A,et al.The E-cadherin adhesion molecule and colorectal cancer.A global literature approach[J].Anticancer Res,2008,28(6A):3815-3826.
[6]Schmalhofer O,Brabletz S,Brabletz T.E-cadherin,beta-catenin,and ZEB1in malignant progression of cancer[J].Cancer Metastasis Rev,2009,28(1-2):151-166.
[7]Chinnadurai G.The transcriptional corepressor CtBP:a foe of multiple tumor suppressors[J].Cancer Res,2009,69(3):731-734.
[8]曾思恩,肖胜军,张小玲,等.肝细胞肝癌中转录辅抑制因子CtBP1与其靶基因E-cadherin的表达[J].临床与实验病理学杂志,2010,26(6):655-657.
[9]Zhang X L,Huang C X,Zhang J,et al.CtBP1is involved in epithelial-mesenchymal transition and is a potential therapeutic target for hepatocellular carcinoma[J].Oncol Rep,2013,30(2):809.
[10]Nadauld L D,Phelps R,Moore B C,et al.Adenomatous polyposis coli control of C-terminal binding protein-1stability regulates expression of intestinal retinol dehydrogenases[J].J Biol Chem,2006,281(49):37828-37835.