离子液体均相液液微萃取法测定豆类中的三嗪类除草剂
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摘要
将离子液体应用于微波辅助液液微萃取高效液相色谱法测定豆类粮食中的四种三嗪类除草剂,包括草净津、特丁通、西玛通、特丁津,并对提取溶剂的种类和体积、样品溶液的pH值、提取时间、微波功率和离心速度等实验条件进行了优化。四种除草剂在0.1~2μg·L~(-1)范围内线性关系良好,检出限分别为8.06、7.17、8.74、7.74μg·L~(-1),加标回收率范围从88%~106%,相对标准偏差均小于4.0%;该方法可萃取测定三种豆子的四种除草剂。
The four triazine herbicides,cyanazine,terbumeton,simetone and terbuthylazine,were determined by the application of ionic liquids in microwave assisted liquid-liquid micro-extraction and high performance liquid chromatography. In the experimental process,the types and volume of the extraction solvent,the pH value of the sample solution,the extraction time,the microwave power and the centrifugal speed were optimized. A good linearity was obtained in four herbicides concentration range of 0.1-2 μg·L~(-1).The detection limits were 8.06,7.17,8.74,7.74 μg·L~(-1) respectively. The recovery of the analytes ranged from 88%-106%,relative standard deviation was less than 4.0%. The proposed method could be used to extract and determine the triazine herbicides in the soybean.
The four triazine herbicides,cyanazine,terbumeton,simetone and terbuthylazine,were determined by the application of ionic liquids in microwave assisted liquid-liquid micro-extraction and high performance liquid chromatography. In the experimental process,the types and volume of the extraction solvent,the pH value of the sample solution,the extraction time,the microwave power and the centrifugal speed were optimized. A good linearity was obtained in four herbicides concentration range of 0.1-2 μg·L~(-1).The detection limits were 8.06,7.17,8.74,7.74 μg·L~(-1) respectively. The recovery of the analytes ranged from 88%-106%,relative standard deviation was less than 4.0%. The proposed method could be used to extract and determine the triazine herbicides in the soybean.
引文
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[8]田香,玉华,白玉霞.HPLC法测定蒙药如达七味散中栀子苷的含量[J].内蒙古民族大学学报,2016(2):152-155.
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[10]Gao S Q,You J Y,Zheng X,et al.Determination of phenylurea and triazine herbicides in milk by microwave assisted ionic liquid microextraction high-performance liquid chromatography[J].Talanta,2010,82:1371-1377.
[11]孙鹏,竺鉴博,高玉玲.离子液体分散液—液微萃取—高效液相色谱法测定碳酸饮料中合成着色剂[J].黑龙江八一农垦大学学报,2017,29(3):65-69.
[2]Wang Z B,Zhang L Y,Li N,et al.Ionic liquid-based matrix solid-phase dispersion coupled with homogeneous liquid-liquid microextraction of synthetic dyes in condiments[J].Journal of Chromatography A,2014,138:52-62.
[3]Zhang L Y,Wang Z B,Li N,et al.Ionic liquid-based foam flotation followed by solid phase extraction to determine triazine herbicides in corn[J].Talanta,2014,122,43-50.
[4]郭晓玉,陈明,张文龙,等.GC/MS分析黄花菜精油挥发性成分[J].延边大学农学学报,2016(1):35-39.
[5]Zhang L Y,Yao D,Yu R Z,et al.Extraction and separation of triazine herbicides in soybean by ionic liquid foam-based solvent flotation and high performance liquid chromatography determination[J].Analytical Methods,2015(7):1977-1983.
[6]叶新强,鲁岩,张恒.除草剂阿拉特津的使用与危害[J].环境科学与管理,2006,31(8):95-97.
[7]张丽媛,姚笛,李娜,等.离子液体均相液液微萃取高效液相色谱法测定婴儿奶粉中5种三嗪类除草剂[J].色谱,2015,33(7):753-758.
[8]田香,玉华,白玉霞.HPLC法测定蒙药如达七味散中栀子苷的含量[J].内蒙古民族大学学报,2016(2):152-155.
[9]祁彦,占春瑞,张新忠,等.高效液相色谱法测定大豆中13种三嗪类除草剂多残留量[J].分析化学,2006,34(6):787-790.
[10]Gao S Q,You J Y,Zheng X,et al.Determination of phenylurea and triazine herbicides in milk by microwave assisted ionic liquid microextraction high-performance liquid chromatography[J].Talanta,2010,82:1371-1377.
[11]孙鹏,竺鉴博,高玉玲.离子液体分散液—液微萃取—高效液相色谱法测定碳酸饮料中合成着色剂[J].黑龙江八一农垦大学学报,2017,29(3):65-69.