摘要
目的:建立复方丹参片中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法,并采用PCA和HCA法进行分析。方法:采用高效液相色谱法,采用AlltimaTM C_(18)(4.6mm×250mm,5μm)Serial No 209050188色谱柱;流动相:乙腈-水;梯度洗脱;检测波长203nm;柱温:30℃;流速:1mL/min;进样量:5μL。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的线性范围分别为0.106~1.06μg(r=0.9994),0.533~5.33μg(r=0.9998),0.0524~0.524μg(r=0.9997),0.582~5.82μg(r=0.9999);平均加样回收率(n=9)分别为98.02%,98.29%,98.72%,99.77%,RSD分别为2.82%,3.59%,2.43%,2.95%。结论:PCA和HCA较全面的反映了复方丹参片样本的信息,评价结果具有一定的客观性,可为复方丹参片的质量控制提供参考。
Objective:To establish the assay method of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in Compound Danshen Tablets by HPLC and PCA and HCA were conducted for analysis.Methods:This assay was performed on AlltimaTM C_(18)(4.6 mm×250 mm,5 μm)Serial No 209050188 column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 1 mL/min.The detection wavelength was 203 nm.The column temperature was 30 ℃.The injection volumn was 5 μL.Results:The liner rangers of notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 were 0.106-1.06 μg(r=0.9994),0.533-5.33 μg(r=0.9998),0.0524-0.524 μg(r=0.9997),0.582-5.82 μg(r=0.9999),respectively.Their average recoveries(n=9)were 98.02%,98.29%,98.72% and 99.77%,corresponding RSD values were 2.82%,3.59%,2.43% and 2.95%,respectively.Conclusion:The analysis of HCA and PCA could comprehensively reflect the samples of Compound Danshen Tablets.The results were objective,which can be applied to the quality control method of panax notoginseng in Compound Danshen Tablets effectively.
引文
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