肉雏鹅源多杀性巴氏杆菌分离鉴定与致病性试验
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摘要
为了鉴定引起肉雏鹅死亡的病原,无菌采集病死肉雏鹅心血、肺脏、肝脏等病料组织中分离到1株病原菌,通过细菌培养特性、形态学观察、生化试验、Kmt2基因序列测序等方法进行鉴定,分离菌株被鉴定为多杀性巴氏杆菌。采用PCR方法、人工感染致病性试验、K-B药敏纸片法分别对分离菌株进行血清型鉴定、致病性检测及耐药性分析。结果显示,多杀性巴氏杆菌血清型为A型,对14日龄肉雏鹅具有很强的致病性,半数致死量(LD_(50))为5.86×10~6 CFU/mL;分离菌株对对头孢曲松、头孢噻呋、恩诺沙星等6种药物高度敏感;对其他药物有不同的程度耐药性。
In order to identify the pathogen causing the death of the meat goose. 1 strain of pathogenic bacteria were isolated from aseptic diseases, such as dead meat, goose, heart, blood, lungs and liver. Bacterial culture characteristics, morphological observation, biochemical test, Kmt2 gene sequencing and other methods were used to identify the isolated pathogen as Pasteurella multocida. Serotype identification, pathogenicity detection and drug resistance analysis were carried out by PCR, artificial infection pathogenicity test and K-B susceptibility paper method. The results showed that serotype A of Pasteurella multocida was highly pathogenic to 14-day-old broiler goslings. The LD_(50) was 5.86×10~6CFU/mL. The isolates were highly sensitive to ceftriaxone, ceftiofur and enrofloxacin, and were resistant to other drugs to varying degrees.
In order to identify the pathogen causing the death of the meat goose. 1 strain of pathogenic bacteria were isolated from aseptic diseases, such as dead meat, goose, heart, blood, lungs and liver. Bacterial culture characteristics, morphological observation, biochemical test, Kmt2 gene sequencing and other methods were used to identify the isolated pathogen as Pasteurella multocida. Serotype identification, pathogenicity detection and drug resistance analysis were carried out by PCR, artificial infection pathogenicity test and K-B susceptibility paper method. The results showed that serotype A of Pasteurella multocida was highly pathogenic to 14-day-old broiler goslings. The LD_(50) was 5.86×10~6CFU/mL. The isolates were highly sensitive to ceftriaxone, ceftiofur and enrofloxacin, and were resistant to other drugs to varying degrees.
引文
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[7]王羽,董文龙,王巍,等.猪源荚膜血清F型多杀性巴氏杆菌分离鉴定[J].中国兽医杂志,2017,53(06):31-33+36+51.
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[10]张召兴,李蕴玉,张香斋,等.一株鹅源多杀性巴氏杆菌TSP-1的分离鉴定与药敏试验[J].河北科技师范学院学报,2017,31(01):48-52.
[2]杨泽晓,刘常钰,王印,等.一株鹅源多杀性巴氏杆菌强毒株的分离鉴定及其血清型鉴定相关基因的克隆[J].浙江农业学报,2016,28(6):935-943.
[3]邹灵秀,朱剑宗,潘雨,等.多杀性巴氏杆菌免疫逃避机制[J].畜牧与兽医,2018,50(10):132-136.
[4]张召兴,耿田田,李蕴玉,等.貉源多杀性巴氏杆菌的分离鉴定与药敏试验[J].中国兽医杂志,2016,52(7):40-42.
[5]刘道泉,刘小龙,程龙飞,等.10株禽源多杀性巴氏杆菌药物敏感性与质粒多样性分析[J].中国家禽,2017,39(13):61-63.
[6]杨楠,吴同垒,王洪彬,等.鹅源多杀性巴氏杆菌的分离鉴定与药物敏感性分析[J].中国家禽,2017,39(22):55-58.
[7]王羽,董文龙,王巍,等.猪源荚膜血清F型多杀性巴氏杆菌分离鉴定[J].中国兽医杂志,2017,53(06):31-33+36+51.
[8]National Committee for Clinical Laboartory Standards,Perfomrance standards for antimicrobial disk and dilution susceptibility test for bacteria isolated from anima1s:approved standard,seconded,NC-CLS document M31-A2.Wayne,USA.2000,49(21)105-126.
[9]刘红玉,邵周伍林,李刚,等.一株鹅源巴氏杆菌的分离鉴定及耐药性分析[J].中国兽医科学,2014,44(02):119-123.
[10]张召兴,李蕴玉,张香斋,等.一株鹅源多杀性巴氏杆菌TSP-1的分离鉴定与药敏试验[J].河北科技师范学院学报,2017,31(01):48-52.