肿瘤患者人附红细胞体感染因素分析
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摘要
目的分析人附红细胞体在肿瘤患者中的流行情况并初步分析感染因素。方法 2016年8月—2018年7月从就诊人群中随机抽样,获得肿瘤组106人和健康体检组114人,采集EDTA-K_2抗凝全血,采用瑞氏染色法和吖啶橙荧光染色法对标本进行染色,以二者皆为阳性确定为人附红细胞体感染,并收集年龄、性别、血细胞计数、干预治疗情况等信息,运用χ~2检验对阳性率进行差异性分析。结果肿瘤组和健康体检组人附红细胞体感染率分别为33.02%和11.40%,差异具有统计学意义(χ~2=15.045,P<0.001);肿瘤患者组人附红细胞体感染率在不同系统肿瘤、是否经过干预治疗、红细胞计数、血小板计数、各年龄分布、性别分布等因素中差异无统计学意义(均P>0.05);在白细胞计数增加组中感染率出现了增高的趋势(χ~2=2.885,P=0.089),健康体检组中儿童与成人人附红细胞体感染率差异无统计学意义(χ~2=0.492,P>0.05)。结论肿瘤患者和健康体检者均存在一定程度的人附红细胞体感染,但肿瘤患者比健康体检者更易感染人附红细胞体;肿瘤患者白细胞计数增加可能存在人附红细胞体感染的情况;肿瘤患者人附红细胞体的感染率与是否经过干预治疗、肿瘤系统类别、红细胞计数、血小板计数、年龄、性别等因素无明显相关;健康体检者人附红细胞体的感染率与年龄无关。
Objective To investigate the epidemic status of Eperythrozoon infection and related infection factors in patients with tumors. Methods A total of 106 patients with cancer and 114 patients of healthy physical examination were recruited by random sampling method from August 2016 to July 2018. The EDTA-K_2 anticoagulant whole blood were collected and stained by Wright staining and acridine orange fluorescence staining to determine the infection of Eperythrozoon. Both of them were positive for Eperythrozoon infection and all the relevant information(age, gender, whole blood count, treatment etc) was collected. Chi-square test were applied to analyze the difference of positive rates. Results The corresponding infection rates in the patients group and health control group were 33.02% and 11.40%, and there were significant statistical difference among them(χ~2=15.045, P<0.001). There was no significant difference in the infection rate of Eperythrozoon in tumor patients in different systemic tumors, intervention treatment, erythrocyte blood count, platelet count, age distribution and gender distribution(all P>0.05). The trend of increased rates of infection in increased white blood cell count group was reflected(χ~2=2.885, P=0.089). The infection rate of Eperythrozoon in healthy persons has no statistical significant difference between children and adults(χ~2=0.492, P>0.05). Conclusion There was mild infection of Eperythrozoon in patients with tumor and healthy people, but the infection rate of patients with tumor was significantly higher than that of healthy people; The tumor patients with the increased WBC count maybe infected with human eperythrozoon; The infection rate of human erythrocyte in patients with tumors is not related to the factors of intervention treatment, tumor system, red blood count, platelet count, age, gender etc. The infection rate of human erythrocytes in healthy subjects was not related to age.
Objective To investigate the epidemic status of Eperythrozoon infection and related infection factors in patients with tumors. Methods A total of 106 patients with cancer and 114 patients of healthy physical examination were recruited by random sampling method from August 2016 to July 2018. The EDTA-K_2 anticoagulant whole blood were collected and stained by Wright staining and acridine orange fluorescence staining to determine the infection of Eperythrozoon. Both of them were positive for Eperythrozoon infection and all the relevant information(age, gender, whole blood count, treatment etc) was collected. Chi-square test were applied to analyze the difference of positive rates. Results The corresponding infection rates in the patients group and health control group were 33.02% and 11.40%, and there were significant statistical difference among them(χ~2=15.045, P<0.001). There was no significant difference in the infection rate of Eperythrozoon in tumor patients in different systemic tumors, intervention treatment, erythrocyte blood count, platelet count, age distribution and gender distribution(all P>0.05). The trend of increased rates of infection in increased white blood cell count group was reflected(χ~2=2.885, P=0.089). The infection rate of Eperythrozoon in healthy persons has no statistical significant difference between children and adults(χ~2=0.492, P>0.05). Conclusion There was mild infection of Eperythrozoon in patients with tumor and healthy people, but the infection rate of patients with tumor was significantly higher than that of healthy people; The tumor patients with the increased WBC count maybe infected with human eperythrozoon; The infection rate of human erythrocyte in patients with tumors is not related to the factors of intervention treatment, tumor system, red blood count, platelet count, age, gender etc. The infection rate of human erythrocytes in healthy subjects was not related to age.
引文
[1] PUNTARIC V,BORC,et al.Eperythrozoonosis in man[J].Lancet,1986,2(8511):868-869.
[2] QIU Y,ZHAO R D,YUN MARK M,et al.Eperythrozoon suis infection enhances atherosclerosis in low-density lipoprotein receptor-deficient mice[J].Int J Clin Exp Pathol,2016,9(12):12481-12491.
[3] HOELZLE L E,ZEDER M,FELDER K M,et al.Pathobiology of Mycoplasma suis[J].Vet J,2014,202(1):20-25.
[4] 卫周涛,倪用强.猪附红细胞体病的研究进展[J].河南畜牧兽医:市场版,2016,37(7):41-43.
[5] 蔡淑梅,安淑娟,金凤玲.吖啶橙荧光染色诊断附红细胞体病研究进展及评价[J].国际检验医学杂志,2013,34(23):3193-3195.
[6] 孙静,薛天阳,许伟,等.儿童人附红细胞体病1例报道并文献复习[J].中国保健营养,2013,23(6):1132-1133.
[7] 甄萍,高志红,胡景玉.血小板增多的人附红细胞体病1例[J].河北医药,2014,36(19):3038-3040.
[8] WANG X,CUI Y,ZHANG Y,et al.Molecular characterization of hemotropic mycoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’) in sheep and goats in China[J].BMC Vet Res,2017,13(1):142.
[9] 赵文军,陈瑶.人附红细胞体病的发病机制研究进展[J].北方药学,2014,11(3):60-61.
[10] 沈志红,李伶俐,冯群灿,等.浙江省东部地区人畜附红细胞体感染情况调查[J].预防医学,2017,29(6):589-591.
[11] 黄河,谢汉国,黄锦源,等.福建省将乐县人附红细胞体感染现状调查[J].中国病原生物学杂志,2013,8(12):2-3.
[12] HASAN M H.Diagnosis of some blood parasites in cattle and sheep in Mosul,Iraq[J].Iraqi J Vet Sci,2014,26:57-61.
[13] 窦宁.猪附红细胞体的形态学观察方法的比较[J].山东畜牧兽医,2014,35(5):18-19.
[14] 刘寰宇,陈瑶.附红细胞体病动物模型研究进展[J].转化医学电子杂志,2015,2(5):148-150.
[15] 韩子强,赵晓辉.中国附红细胞体感染流行病学Meta分析[J].医学动物防制,2014,30(4):363-365.
[16] 柴珂,白苏日娜,陈瑶.瑞氏染色法检测人附红细胞体出现假阳性的原因分析[J].检验医学与临床,2017,14(8):1191-1193.
[17] 路凌怡,翟元琦,王剑飚.血液病患者人附红细胞体感染调查[J].检验医学,2017,32(1):26-29.
[18] 赵晓辉,韩子强.人附红细胞体感染小鼠外周血白细胞凋亡的研究[J].中国现代医生,2014,52(13):7-9.
[19] 赵文军,陈瑶,刘志跃,等.人附红细胞体病机体免疫功能变化的研究[J].中国病理生理杂志,2015,31(10):1854.
[2] QIU Y,ZHAO R D,YUN MARK M,et al.Eperythrozoon suis infection enhances atherosclerosis in low-density lipoprotein receptor-deficient mice[J].Int J Clin Exp Pathol,2016,9(12):12481-12491.
[3] HOELZLE L E,ZEDER M,FELDER K M,et al.Pathobiology of Mycoplasma suis[J].Vet J,2014,202(1):20-25.
[4] 卫周涛,倪用强.猪附红细胞体病的研究进展[J].河南畜牧兽医:市场版,2016,37(7):41-43.
[5] 蔡淑梅,安淑娟,金凤玲.吖啶橙荧光染色诊断附红细胞体病研究进展及评价[J].国际检验医学杂志,2013,34(23):3193-3195.
[6] 孙静,薛天阳,许伟,等.儿童人附红细胞体病1例报道并文献复习[J].中国保健营养,2013,23(6):1132-1133.
[7] 甄萍,高志红,胡景玉.血小板增多的人附红细胞体病1例[J].河北医药,2014,36(19):3038-3040.
[8] WANG X,CUI Y,ZHANG Y,et al.Molecular characterization of hemotropic mycoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’) in sheep and goats in China[J].BMC Vet Res,2017,13(1):142.
[9] 赵文军,陈瑶.人附红细胞体病的发病机制研究进展[J].北方药学,2014,11(3):60-61.
[10] 沈志红,李伶俐,冯群灿,等.浙江省东部地区人畜附红细胞体感染情况调查[J].预防医学,2017,29(6):589-591.
[11] 黄河,谢汉国,黄锦源,等.福建省将乐县人附红细胞体感染现状调查[J].中国病原生物学杂志,2013,8(12):2-3.
[12] HASAN M H.Diagnosis of some blood parasites in cattle and sheep in Mosul,Iraq[J].Iraqi J Vet Sci,2014,26:57-61.
[13] 窦宁.猪附红细胞体的形态学观察方法的比较[J].山东畜牧兽医,2014,35(5):18-19.
[14] 刘寰宇,陈瑶.附红细胞体病动物模型研究进展[J].转化医学电子杂志,2015,2(5):148-150.
[15] 韩子强,赵晓辉.中国附红细胞体感染流行病学Meta分析[J].医学动物防制,2014,30(4):363-365.
[16] 柴珂,白苏日娜,陈瑶.瑞氏染色法检测人附红细胞体出现假阳性的原因分析[J].检验医学与临床,2017,14(8):1191-1193.
[17] 路凌怡,翟元琦,王剑飚.血液病患者人附红细胞体感染调查[J].检验医学,2017,32(1):26-29.
[18] 赵晓辉,韩子强.人附红细胞体感染小鼠外周血白细胞凋亡的研究[J].中国现代医生,2014,52(13):7-9.
[19] 赵文军,陈瑶,刘志跃,等.人附红细胞体病机体免疫功能变化的研究[J].中国病理生理杂志,2015,31(10):1854.