致病性李斯特菌毒力因子蛋白组学及基因组学研究进展
|
摘要
单增李斯特菌与绵羊李斯特菌是李斯特菌属中具有致病性的两个菌种,均能在胞内繁殖,可以作为疫苗载体构建疫苗。但它们在宿主范围和致病力等方面又存在较大差异,导致两者在疫苗学和免疫学领域的应用不尽相同。从分子层面上看,两种菌独特的病原学特点与各自的主要毒力因子蛋白及相关毒力基因的差异有关。分析比较两者的毒力因子蛋白及基因,对两菌进一步在疫苗学上的应用很有必要。本文特对单增李斯特菌与绵羊李斯特菌毒力因子蛋白组学和基因组学研究进展作一综述。
Listeria monocytogenes and Listeria ivanovii are the two pathogenic species in genus Listeria. These pathogenic species can multiply in cytoplasm and be used as vaccine vehicles for vaccines construction. However, there are distinct differences on their host range, virulence and other aspects, leading to different applications in the field of vaccinology and immunology. On the perspective of molecular biology, the distinct pathogenic features are associated with the differences of their respective major virulence factors and related genes. Comparing the virulence factors and related genes between the two bacteria strains is very necessary for the application in vaccinology. In this article, proteomic and genomic research advances on Listeria monocytogenes and Listeria ivanovii virulence factors are reviewed.
Listeria monocytogenes and Listeria ivanovii are the two pathogenic species in genus Listeria. These pathogenic species can multiply in cytoplasm and be used as vaccine vehicles for vaccines construction. However, there are distinct differences on their host range, virulence and other aspects, leading to different applications in the field of vaccinology and immunology. On the perspective of molecular biology, the distinct pathogenic features are associated with the differences of their respective major virulence factors and related genes. Comparing the virulence factors and related genes between the two bacteria strains is very necessary for the application in vaccinology. In this article, proteomic and genomic research advances on Listeria monocytogenes and Listeria ivanovii virulence factors are reviewed.
引文
[1]Vázquez-Boland JA,Kuhn M,Berche P,et al.Listeria pathogenesis and molecular virulence determinants[J].Clin Microbiol Rev,2001,14(3):584-640.
[2]FENG YF,WU SY,Varma JK,et al.Systematic review of human listeriosis in China,1964-2010[J].Tropical Medicine&International Health,2013,18(10):1248-1256.
[3]Guillet C,Join-Lambert O,Le Monnier A,et al.Human listeriosis caused by Listeria ivanovii[J].Emerg Infect Dis,2010,16(1):136-138.
[4]Mainou-Fowler T,Macgowan AP,Postlethwaite R.Virulence of listeria spp:course of infection in resistant and susceptible mice[J].J Med Microbiol,1988,27(2):131-140.
[5]Cossart P.Illuminating the landscape of host-pathogen interactions with the bacterium Listeria monocytogenes[J].Proc Natl Acad Sci U S A,2011,108(49):19484-19491.
[6]Singh R,Wallecha A.Cancer immunotherapy using recombinant Listeria monocytogenes:transition from bench to clinic[J].Hum Vaccin,2011,7(5):497-505.
[7]Glomski IJ,Decatur AL,Portnoy DA.Listeria monocytogenes mutants that fail to compartmentalize listerolysin O activity are cytotoxic,avirulent,and unable to evade host extracellular defenses[J].Infect Immun,2003,71(12):6754-6765.
[8]Hamon MA,Ribet D,Stavru F,et al.Listeriolysin O:the Swiss army knife of Listeria[J].Trends Microbiol,2012,20(8):360-368.
[9]Vazquez-Boland JA,Dominguez L,Rodriguez-Ferri EF,et al.Purification and characterization of two Listeria ivanovii cytolysins,a sphingomyelinase C and a thiol-activated toxin(ivanolysin O)[J].Infect Immun,1989,57(12):3928-3935.
[10]Frehel C,Lety MA,Autret N,et al.Capacity of ivanolysin O to replace listeriolysin O in phagosomal escape and in vivo survival of Listeria monocytogenes[J].Microbiology,2003,149(Pt 3):611-620.
[11]Kimoto T,Kawamura I,Kohda C,et al.Differences in gamma interferon production induced by listeriolysin O and lvanolysin O result in different levels of protective immunity in mice infected with Listeria monocytogenes and Listeria ivanovii[J].Infect Immun,2003,71(5):2447-2454.
[12]Vazquez-Boland JA,Kocks C,Dramsi S,et al.Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread[J].Infect Immun,1992,60(1):219-230.
[13]Camejo A,Carvalho F,Reis O,et al.The Arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle[J].Virulence,2011,2(5):379-394.
[14]Goldfine H,Knob C.Purification and characterization of Listeria monocytogenes phosphatidylinositol-specific phospholipase C[J].Infect Immun,1992,60(10):4059-4067.
[15]Gonzalez-Zorn B,Dominguez-Bernal G,Suarez M,et al.The smcL gene of Listeria ivanovii encodes a sphingomyelinase C that mediates bacterial escape from the phagocytic vacuole[J].Mol Microbiol,1999,33(3):510-523.
[16]Gonzalez ZB,Dominguez BG,Suarez M,et al.SmcL,a novel membrane-damaging virulence factor in Listeria[J].International Journal of Medical Microbiology,2000,290(4):369-374.
[17]Boujemaa-Paterski R,Gouin E,Hansen G,et al.Listeria protein ActA mimics WASp family proteins:it activates filament barbed end branching by Arp2/3 complex[J].Biochemistry,2001,40(38):11390-11404.
[18]Travier L,Guadagnini S,Gouin E,et al.ActA promotes Listeria monocytogenes aggregation,intestinal colonization and carriage[J].PLoS Pathog,2013,9(1):e1003131.
[19]Gouin E,Dehoux P,Mengaud J,et al.iactA of listeria ivanovii,although distantly related to listeria monocytogenes actA,restores actin tail formation in an L.monocytogenes actA mutant[J].Infect Immun,1995,63(7):2729-2737.
[20]Lecuit M,Nelson DM,Smith SD,et al.Targeting and crossing of the human maternofetal barrier by Listeria monocytogenes:Role of internalin interaction with trophoblast E-cadherin[J].Proc Natl Acad Sci U S A,2004,101(16):6152-6157.
[21]Pizarro-CerdáJ,Kühbacher A,Cossart P.Entry of listeria monocytogenes in mammalian epithelial cells:an updated view[J].Cold Spring Harb Perspect Med,2012,2(11):a010009.
[22]Raffelsbauer D,Bubert A,Engelbrecht F,et al.The gene cluster inlC 2DE of Listeria monocytogenes contains additional new internalin genes and is important for virulence in mice[J].Molecular and General Genetics MGG,1998,260(2/3):144-158.
[23]Engelbrecht F,Chun Sk,Ochs C,et al.A new PrfA-regulated gene of Listeria monocytogenes encoding a small,secreted protein which belongs to the family of internalins[J].Mol Microbiol,1996,21(4):823-837.
[24]Engelbrecht F,Domínguez-Bernal G,Hess J,et al.A novel PrfA-regulated chromosomal locus,which is specific for Listeria ivanovii,encodes two small,secreted internalins and contributes to virulence in mice[J].Mol Microbiol,1998,30(2):405-417.
[25]Domínguez-Bernal G,Müller-Altrock S,González-Zorn B,et al.A spontaneous genomic deletion in Listeria ivanovii identifies LIPI-2,a species-specific pathogenicity island encoding sphingomyelinase and numerous internalins[J].Mol Microbiol,2006,59(2):415-432.
[26]Vázquez-Boland JA,Domínguez-Bernal G,González-Zorn B,et al.Pathogenicity islands and virulence evolution in Listeria[J].Microbes Infect,2001,3(7):571-584.
[27]Chakraborty T,Hain T,Domann E.Genome organization and the evolution of the virulence gene locus in Listeria species[J].International Journal of Medical Microbiology,2000,290(2):167-174.
[28]Schmid MW,Ng EY,Lampidis R,et al.Evolutionary history of the genus Listeria and its virulence genes[J].Syst Appl Microbiol,2005,28(1):1-18.
[29]Hacker J,Blum-Oehler G,Hochhut B,et al.The molecular basis of infectious diseases:pathogenicity islands and other Mobile genetic elements[J].Acta Microbiol Immunol Hung,2003,50(4):321-330.
[30]Hodgson DA.Generalized transduction of serotype 1/2 and serotype4b strains of Listeria monocytogenes[J].Mol Microbiol,2000,35(2):312-323.
[31]De Las Heras A,Cain RJ,Bielecka MK,et al.Regulation of listeria virulence:PrfA master and commander[J].Curr Opin Microbiol,2011,14(2):118-127.
[32]Sheehan B,Klarsfeld A,Ebright R,et al.A single substitution in the putative helix-turn-helix motif of the pleiotropic activator PrfA attenuates Listeria monocytogenes virulence[J].Mol Microbiol,1996,20(4):785-797.
[33]Mauder N,Ecke R,Mertins S,et al.Species-specific differences in the activity of PrfA,the key regulator of listerial virulence genes[J].J Bacteriol,2006,188(22):7941-7956.
[2]FENG YF,WU SY,Varma JK,et al.Systematic review of human listeriosis in China,1964-2010[J].Tropical Medicine&International Health,2013,18(10):1248-1256.
[3]Guillet C,Join-Lambert O,Le Monnier A,et al.Human listeriosis caused by Listeria ivanovii[J].Emerg Infect Dis,2010,16(1):136-138.
[4]Mainou-Fowler T,Macgowan AP,Postlethwaite R.Virulence of listeria spp:course of infection in resistant and susceptible mice[J].J Med Microbiol,1988,27(2):131-140.
[5]Cossart P.Illuminating the landscape of host-pathogen interactions with the bacterium Listeria monocytogenes[J].Proc Natl Acad Sci U S A,2011,108(49):19484-19491.
[6]Singh R,Wallecha A.Cancer immunotherapy using recombinant Listeria monocytogenes:transition from bench to clinic[J].Hum Vaccin,2011,7(5):497-505.
[7]Glomski IJ,Decatur AL,Portnoy DA.Listeria monocytogenes mutants that fail to compartmentalize listerolysin O activity are cytotoxic,avirulent,and unable to evade host extracellular defenses[J].Infect Immun,2003,71(12):6754-6765.
[8]Hamon MA,Ribet D,Stavru F,et al.Listeriolysin O:the Swiss army knife of Listeria[J].Trends Microbiol,2012,20(8):360-368.
[9]Vazquez-Boland JA,Dominguez L,Rodriguez-Ferri EF,et al.Purification and characterization of two Listeria ivanovii cytolysins,a sphingomyelinase C and a thiol-activated toxin(ivanolysin O)[J].Infect Immun,1989,57(12):3928-3935.
[10]Frehel C,Lety MA,Autret N,et al.Capacity of ivanolysin O to replace listeriolysin O in phagosomal escape and in vivo survival of Listeria monocytogenes[J].Microbiology,2003,149(Pt 3):611-620.
[11]Kimoto T,Kawamura I,Kohda C,et al.Differences in gamma interferon production induced by listeriolysin O and lvanolysin O result in different levels of protective immunity in mice infected with Listeria monocytogenes and Listeria ivanovii[J].Infect Immun,2003,71(5):2447-2454.
[12]Vazquez-Boland JA,Kocks C,Dramsi S,et al.Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread[J].Infect Immun,1992,60(1):219-230.
[13]Camejo A,Carvalho F,Reis O,et al.The Arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle[J].Virulence,2011,2(5):379-394.
[14]Goldfine H,Knob C.Purification and characterization of Listeria monocytogenes phosphatidylinositol-specific phospholipase C[J].Infect Immun,1992,60(10):4059-4067.
[15]Gonzalez-Zorn B,Dominguez-Bernal G,Suarez M,et al.The smcL gene of Listeria ivanovii encodes a sphingomyelinase C that mediates bacterial escape from the phagocytic vacuole[J].Mol Microbiol,1999,33(3):510-523.
[16]Gonzalez ZB,Dominguez BG,Suarez M,et al.SmcL,a novel membrane-damaging virulence factor in Listeria[J].International Journal of Medical Microbiology,2000,290(4):369-374.
[17]Boujemaa-Paterski R,Gouin E,Hansen G,et al.Listeria protein ActA mimics WASp family proteins:it activates filament barbed end branching by Arp2/3 complex[J].Biochemistry,2001,40(38):11390-11404.
[18]Travier L,Guadagnini S,Gouin E,et al.ActA promotes Listeria monocytogenes aggregation,intestinal colonization and carriage[J].PLoS Pathog,2013,9(1):e1003131.
[19]Gouin E,Dehoux P,Mengaud J,et al.iactA of listeria ivanovii,although distantly related to listeria monocytogenes actA,restores actin tail formation in an L.monocytogenes actA mutant[J].Infect Immun,1995,63(7):2729-2737.
[20]Lecuit M,Nelson DM,Smith SD,et al.Targeting and crossing of the human maternofetal barrier by Listeria monocytogenes:Role of internalin interaction with trophoblast E-cadherin[J].Proc Natl Acad Sci U S A,2004,101(16):6152-6157.
[21]Pizarro-CerdáJ,Kühbacher A,Cossart P.Entry of listeria monocytogenes in mammalian epithelial cells:an updated view[J].Cold Spring Harb Perspect Med,2012,2(11):a010009.
[22]Raffelsbauer D,Bubert A,Engelbrecht F,et al.The gene cluster inlC 2DE of Listeria monocytogenes contains additional new internalin genes and is important for virulence in mice[J].Molecular and General Genetics MGG,1998,260(2/3):144-158.
[23]Engelbrecht F,Chun Sk,Ochs C,et al.A new PrfA-regulated gene of Listeria monocytogenes encoding a small,secreted protein which belongs to the family of internalins[J].Mol Microbiol,1996,21(4):823-837.
[24]Engelbrecht F,Domínguez-Bernal G,Hess J,et al.A novel PrfA-regulated chromosomal locus,which is specific for Listeria ivanovii,encodes two small,secreted internalins and contributes to virulence in mice[J].Mol Microbiol,1998,30(2):405-417.
[25]Domínguez-Bernal G,Müller-Altrock S,González-Zorn B,et al.A spontaneous genomic deletion in Listeria ivanovii identifies LIPI-2,a species-specific pathogenicity island encoding sphingomyelinase and numerous internalins[J].Mol Microbiol,2006,59(2):415-432.
[26]Vázquez-Boland JA,Domínguez-Bernal G,González-Zorn B,et al.Pathogenicity islands and virulence evolution in Listeria[J].Microbes Infect,2001,3(7):571-584.
[27]Chakraborty T,Hain T,Domann E.Genome organization and the evolution of the virulence gene locus in Listeria species[J].International Journal of Medical Microbiology,2000,290(2):167-174.
[28]Schmid MW,Ng EY,Lampidis R,et al.Evolutionary history of the genus Listeria and its virulence genes[J].Syst Appl Microbiol,2005,28(1):1-18.
[29]Hacker J,Blum-Oehler G,Hochhut B,et al.The molecular basis of infectious diseases:pathogenicity islands and other Mobile genetic elements[J].Acta Microbiol Immunol Hung,2003,50(4):321-330.
[30]Hodgson DA.Generalized transduction of serotype 1/2 and serotype4b strains of Listeria monocytogenes[J].Mol Microbiol,2000,35(2):312-323.
[31]De Las Heras A,Cain RJ,Bielecka MK,et al.Regulation of listeria virulence:PrfA master and commander[J].Curr Opin Microbiol,2011,14(2):118-127.
[32]Sheehan B,Klarsfeld A,Ebright R,et al.A single substitution in the putative helix-turn-helix motif of the pleiotropic activator PrfA attenuates Listeria monocytogenes virulence[J].Mol Microbiol,1996,20(4):785-797.
[33]Mauder N,Ecke R,Mertins S,et al.Species-specific differences in the activity of PrfA,the key regulator of listerial virulence genes[J].J Bacteriol,2006,188(22):7941-7956.