绿萝对香烟烟雾的净化与生理响应
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摘要
为了研究室内观赏植物绿萝(Epipremnum aureum)对香烟烟雾的净化能力及其生长生理响应,从而为香烟烟雾污染防控提供依据,本研究模拟人的吸烟方式,在长、宽、高均为60 cm,密闭或间隔通风的玻璃装置中注入2支香烟,放置绿萝。监测72 h内CO和PM2.5浓度变化,观察植物的表型变化,测定植物叶片生理指标变化。结果表明,绿萝可有效吸收或吸附香烟烟雾产生的CO和PM2.5,净化空气;香烟烟雾处理对植物生长产生了一定的影响,叶片出现水渍状斑块,随时间延长,整叶发黄;植物叶片活性氧(超氧阴离子和过氧化氢)水平显著增加,造成膜脂过氧化加剧,丙二醛含量显著增加;植物启动抗氧化酶系统及时清除活性氧;随胁迫时间延长,抗性减弱。通风可有效加速空气流动,减轻香烟烟雾对植物的伤害。因此,建议在吸烟区摆放绿萝并时常通风,可有效降低香烟烟雾污染带来的风险。
To investigate the purification capacity of indoor ornamental plant Epipremnum aureum on cigarette smoke and its growth and physiological responses, and provide a basis for prevention and control of cigarette smoke pollution, cigarette smoking way of mankind was imitated in this study, two cigarettes were injected into a sealed or interval ventilated glass capsule with length, width and height all of 60 cm, and Epipremnum aureum was put into the capsule. CO and PM2.5 concentrations during 72 h were measured, changes of phenotypes were observed and the physiological indexes in leaves were determined. The results showed that: E.aureum could effectively absorb CO and PM2.5 produced by cigarette smoke and purify the air. Plant growth was affected by cigarette smoke treatments to some degree. Watery plaque spots were appeared in leaves and the whole leaves became yellow with the time prolonging. Levels of reactive oxygen species(including superoxide anion and hydrogen peroxide) in leaves were significantly increased, resulting in aggravation of membrane lipid peroxidation and the increase of malondialdehyde content. The plant triggered antioxidant enzymes system to eliminate reactive oxygen species in time; while, as the time went on, the resistance became weakened.Ventilation effectively accelerated air flow and alleviated the cigarette smoke toxicity to plants. Therefore, we suggest E. aureum should be put in smoking area and ventilating sometimes, thus it will decrease the risk caused by cigarette smoke pollution.
To investigate the purification capacity of indoor ornamental plant Epipremnum aureum on cigarette smoke and its growth and physiological responses, and provide a basis for prevention and control of cigarette smoke pollution, cigarette smoking way of mankind was imitated in this study, two cigarettes were injected into a sealed or interval ventilated glass capsule with length, width and height all of 60 cm, and Epipremnum aureum was put into the capsule. CO and PM2.5 concentrations during 72 h were measured, changes of phenotypes were observed and the physiological indexes in leaves were determined. The results showed that: E.aureum could effectively absorb CO and PM2.5 produced by cigarette smoke and purify the air. Plant growth was affected by cigarette smoke treatments to some degree. Watery plaque spots were appeared in leaves and the whole leaves became yellow with the time prolonging. Levels of reactive oxygen species(including superoxide anion and hydrogen peroxide) in leaves were significantly increased, resulting in aggravation of membrane lipid peroxidation and the increase of malondialdehyde content. The plant triggered antioxidant enzymes system to eliminate reactive oxygen species in time; while, as the time went on, the resistance became weakened.Ventilation effectively accelerated air flow and alleviated the cigarette smoke toxicity to plants. Therefore, we suggest E. aureum should be put in smoking area and ventilating sometimes, thus it will decrease the risk caused by cigarette smoke pollution.
引文
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[6]孙咏梅,戴树桂,袭著革.香烟烟雾成分分析及其对DNA生物氧化能力研究[J].环境与健康杂志,2001,18(4):203-207
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[8]张静,仲春雪,黄云飞,等.香烟烟雾暴露对大鼠睾丸组织雄激素结合蛋白m RNA及蛋白表达水平的影响[J].环境与健康杂志,2017,34(5):398-401.
[9] Dasgupta P, Rizwani W, Pillai S, et al. Nicotine induces cell proliferation, invasion and epithelial-mesenchymal transition in a variety of human cancer cell lines[J]. International Journal of Cancer, 2009,124:36-45.
[10] Liu Y, Liu B A. Enhanced proliferation, invasion, and epithelialmesenchymal transition of nicotine-promoted gastric cancer by perionstin[J]. World Journal of Gastroenterology, 2011,17(21):2674-2680.
[11] Nakada T, Kiyotani K, Iwano S, et al. Lung tumorigenesis promoted by anti-apoptotic effects of cotinine, a nicotine metabolite through activation of PI3K/Akt pathway[J]. Journal of Toxicological Sciences, 2012,37(3):555-563.
[12]孙晓花,宋海东,郑美春,等.环境香烟烟雾暴露与儿童注意缺陷多动障碍的相关性研究[J].中国预防医学杂志,2014,15(8):737-739.
[13]马铮,平芬,曹磊,等.香烟烟雾成分对呼吸系统的影响[J].国际呼吸杂志,2015,35(4):313-315.
[14]王雪梅,高英,马倩芸,等.香烟烟雾对卵巢功能的影响[J].四川生理科学杂志,2015,37(3):135-137.
[15] Toda N, Okamura T. Cigarette smoking impairs nitric oxidemediated cerebral blood flow increase:Implications for Alzheimer's disease[J]. Journal of Pharmacological Sciences, 2016,131:223-232.
[16] Sriprapat W, Suksabye P, Areephak S, et al. Uptake of toluene and ethylbenzene by plants:Removal of volatile indoor air ontaminants[J]. Ecotoxicology and Environmental Safety, 2014,102:147-151.
[17]倪菲菲,谢平凡,李金炜,等.绿色植物对室内空气污染的净化作用[J].中国农学通报,2017,33(4):91-97.
[18]崔洪珊,贾伟.绿萝等6种室内观赏植物对氨气净化作用分析[J].湖南理工学院学报:自然科学版,2015,28(4):60-62.
[19]王彦靖,解娇,高海,等.9种室内观赏植物对苯的净化能力[J].福建林业科技,2014,41(1):60-62,107.
[20]陈佳瀛,邵勤龙,俞璟凤,等.6种室内观赏植物的甲醛净化效果比较[J].广东农业科学,2014(1):27-32.
[21]武乾秀.绿萝去除低浓度生活污水COD效应的研究[J].化工设计通讯,2016,42(7):125-125.
[22]解淑娟.绿萝对(Epiprem numaureum)对重金属的富集特性及相关机制的研究[D].青岛:中国海洋大学,2015.
[23]郭晖,冯文君,朱红霞,等.土壤盐胁迫下4种园林植物的生理抗性[J].江苏农业科学,2017,45(14):115-118.
[24]张永芳.被动吸烟对小白鼠部分器官组织形态学上的影响[D].大连:辽宁师范大学,2009.
[25]胡洪营.环境工程原理(第三版)[M].北京:高等教育出版社,2015.
[26]李小方,张志良.植物生理学实验指导(第5版)[M].北京:高等教育出版社,2016:211-213.
[27] Mishra S K S D, Singhal G S. Interrelationship between salt and light stress on the primary process of photosynthesis[J]. Journal of Plant Physiology, 1991,138:92-96.
[28] Jana S C M. Glycolate metabolism of three submerged aquatic angiosperms during aging[J]. Aquatic Botany, 1981,12:345-354.
[29] Beauchamp C, Fridovich I. Superoxide dismutase:improved assays and an assay applicable to acrylamide gels[J]. Analytical Biochemistry, 1971,44:276-287.
[30] Rao M V, Paliyath G, Ormrod D P, et al. Influence of salicylic acid on H2O2production, oxidative stress, and H2O2-metabolizing enzymes. Salicylic acid-mediated oxidative damage requires H2O2[J]. Plant Physiology, 1997,115:137-149.
[31] Aebi H E. Catalase. In:Methods of Enzymatic Analysis[M].Weinheim:Verlag Chemie., 1983,273-285.
[32] Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbatespecific peroxidase in spinach chloroplasts[J]. Plant Cell and Physiology, 1981,22:867-880.
[33]张淑娟,黄耀棠.利用植物净化室内甲醛污染的研究进展[J].生态环境学报,2010,19(12):3006-3013.
[34] dela Cruz M, Christensen J H, Thomsen J D, et al. Can ornamental potted plants remove volatile organic compounds from indoor air?A review[J]. Environmental Science and Pollution Research, 2014,21(24):13909-13928.
[35] Sriprapat W, Boraphech P, Thiravetyan P. Factors affecting xylenecontaminated air removal by the ornamental plant Zamioculcas zamiifolia[J]. Environmental Science and Pollution Research, 2014,21:2603-2610.
[36]梁鸣,佟斌,宫伟.4种室内观赏植物对香烟烟雾的净化效果及生理指标变化[J].国土与自然资源研究,2015(5):82-84.
[37]欧佳,薛勇,武海英,等.观赏植物吸收室内甲醛的实验研究[J].资源与人居环境, 2008(22):68-70.
[2]董捷,林勇,孙军,等.济南市室内环境质量变化趋势与特征污染物研究[A].2013中国环境科学学会学术年会论文集(第四卷)[C].中国环境科学学会,2013:3.
[3] Talhout R, Schulz T, Florek E, et al. Hazardous compounds in tobacco smoke[J]. International Journal of Environmental Research and Public Health, 2011, 8:613–628.
[4]周闰.香烟烟雾对室内空气污染及其对健康影响的研究进展[J].职业与健康,2014,30(16):2346-2348.
[5] da Silveira Fleck A, Carneiro M F H, Barbosa F J, et al. Monitoring an outdoor smoking area by means of PM2.5measurement and vegetal biomonitoring[J]. Environmental Science and Pollution research, 2016, 23:21187-21194.
[6]孙咏梅,戴树桂,袭著革.香烟烟雾成分分析及其对DNA生物氧化能力研究[J].环境与健康杂志,2001,18(4):203-207
[7]甘德坤,焦力,朴文花,等.环境香烟烟雾对非吸烟人群健康影响研究进展[J].环境与健康杂志,2000,17(2):125-128.
[8]张静,仲春雪,黄云飞,等.香烟烟雾暴露对大鼠睾丸组织雄激素结合蛋白m RNA及蛋白表达水平的影响[J].环境与健康杂志,2017,34(5):398-401.
[9] Dasgupta P, Rizwani W, Pillai S, et al. Nicotine induces cell proliferation, invasion and epithelial-mesenchymal transition in a variety of human cancer cell lines[J]. International Journal of Cancer, 2009,124:36-45.
[10] Liu Y, Liu B A. Enhanced proliferation, invasion, and epithelialmesenchymal transition of nicotine-promoted gastric cancer by perionstin[J]. World Journal of Gastroenterology, 2011,17(21):2674-2680.
[11] Nakada T, Kiyotani K, Iwano S, et al. Lung tumorigenesis promoted by anti-apoptotic effects of cotinine, a nicotine metabolite through activation of PI3K/Akt pathway[J]. Journal of Toxicological Sciences, 2012,37(3):555-563.
[12]孙晓花,宋海东,郑美春,等.环境香烟烟雾暴露与儿童注意缺陷多动障碍的相关性研究[J].中国预防医学杂志,2014,15(8):737-739.
[13]马铮,平芬,曹磊,等.香烟烟雾成分对呼吸系统的影响[J].国际呼吸杂志,2015,35(4):313-315.
[14]王雪梅,高英,马倩芸,等.香烟烟雾对卵巢功能的影响[J].四川生理科学杂志,2015,37(3):135-137.
[15] Toda N, Okamura T. Cigarette smoking impairs nitric oxidemediated cerebral blood flow increase:Implications for Alzheimer's disease[J]. Journal of Pharmacological Sciences, 2016,131:223-232.
[16] Sriprapat W, Suksabye P, Areephak S, et al. Uptake of toluene and ethylbenzene by plants:Removal of volatile indoor air ontaminants[J]. Ecotoxicology and Environmental Safety, 2014,102:147-151.
[17]倪菲菲,谢平凡,李金炜,等.绿色植物对室内空气污染的净化作用[J].中国农学通报,2017,33(4):91-97.
[18]崔洪珊,贾伟.绿萝等6种室内观赏植物对氨气净化作用分析[J].湖南理工学院学报:自然科学版,2015,28(4):60-62.
[19]王彦靖,解娇,高海,等.9种室内观赏植物对苯的净化能力[J].福建林业科技,2014,41(1):60-62,107.
[20]陈佳瀛,邵勤龙,俞璟凤,等.6种室内观赏植物的甲醛净化效果比较[J].广东农业科学,2014(1):27-32.
[21]武乾秀.绿萝去除低浓度生活污水COD效应的研究[J].化工设计通讯,2016,42(7):125-125.
[22]解淑娟.绿萝对(Epiprem numaureum)对重金属的富集特性及相关机制的研究[D].青岛:中国海洋大学,2015.
[23]郭晖,冯文君,朱红霞,等.土壤盐胁迫下4种园林植物的生理抗性[J].江苏农业科学,2017,45(14):115-118.
[24]张永芳.被动吸烟对小白鼠部分器官组织形态学上的影响[D].大连:辽宁师范大学,2009.
[25]胡洪营.环境工程原理(第三版)[M].北京:高等教育出版社,2015.
[26]李小方,张志良.植物生理学实验指导(第5版)[M].北京:高等教育出版社,2016:211-213.
[27] Mishra S K S D, Singhal G S. Interrelationship between salt and light stress on the primary process of photosynthesis[J]. Journal of Plant Physiology, 1991,138:92-96.
[28] Jana S C M. Glycolate metabolism of three submerged aquatic angiosperms during aging[J]. Aquatic Botany, 1981,12:345-354.
[29] Beauchamp C, Fridovich I. Superoxide dismutase:improved assays and an assay applicable to acrylamide gels[J]. Analytical Biochemistry, 1971,44:276-287.
[30] Rao M V, Paliyath G, Ormrod D P, et al. Influence of salicylic acid on H2O2production, oxidative stress, and H2O2-metabolizing enzymes. Salicylic acid-mediated oxidative damage requires H2O2[J]. Plant Physiology, 1997,115:137-149.
[31] Aebi H E. Catalase. In:Methods of Enzymatic Analysis[M].Weinheim:Verlag Chemie., 1983,273-285.
[32] Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbatespecific peroxidase in spinach chloroplasts[J]. Plant Cell and Physiology, 1981,22:867-880.
[33]张淑娟,黄耀棠.利用植物净化室内甲醛污染的研究进展[J].生态环境学报,2010,19(12):3006-3013.
[34] dela Cruz M, Christensen J H, Thomsen J D, et al. Can ornamental potted plants remove volatile organic compounds from indoor air?A review[J]. Environmental Science and Pollution Research, 2014,21(24):13909-13928.
[35] Sriprapat W, Boraphech P, Thiravetyan P. Factors affecting xylenecontaminated air removal by the ornamental plant Zamioculcas zamiifolia[J]. Environmental Science and Pollution Research, 2014,21:2603-2610.
[36]梁鸣,佟斌,宫伟.4种室内观赏植物对香烟烟雾的净化效果及生理指标变化[J].国土与自然资源研究,2015(5):82-84.
[37]欧佳,薛勇,武海英,等.观赏植物吸收室内甲醛的实验研究[J].资源与人居环境, 2008(22):68-70.