柑橘果胶Ag纳米粒子的绿色制备及表征
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摘要
Ag纳米粒子(Ag NPs)具有广谱抑菌性和对哺乳动物细胞的低毒性,已得到广泛地认可和应用。目前Ag NPs的制备工艺繁琐,所用化学试剂易造成环境污染。本文以天然生物大分子柑橘果胶为还原剂和稳定剂,研究Ag NPs绿色制备工艺。用酸提醇析法提取橘皮果胶,得率24.2%,半乳糖醛酸含量73.5%,酯化度75.6%;以其为原材料,优化的柑橘果胶Ag NPs的制备工艺:用Na OH(20 g/L)溶解制备果胶溶液(50 m L,3 g/L),在温度70℃条件下滴入硝酸银(1 m L,0.5 mo/L)反应1 min。紫外-可见光谱分析该纳米粒子在410 nm处较强吸收。透射电子显微镜(TEM)观察结果表明:制备的Ag NPs平均粒径为5~15 nm,具有窄的粒度分布。扫描电镜(SEM)对制备的Ag NPs进行表征,表明该纳米粒子呈均匀球形。本研究提供了一种Ag NPs的绿色合成工艺,为抗菌材料的开发利用提供理论参考。
Ag nanoparticles(Ag NPs) have broad-spectrum antibacterial properties and low toxicity to mammalian cells, which have been widely recognized and applied. However, the preparation process of Ag NPs was tedious, and the chemical reagents used were easy to cause environmental pollution. So in this experiment, the green synthesis of Ag NPs was studied by using natural biological macromolecules citrus pectin as reducing agent and stabilizer. In this experiment,orange peel pectin was extracted by acid extract and ethanol precipitation, the yield of pectin was 24.2%, the content of glucuronic acid was 73.5%, and the esterification degree was 75.6%. The green synthesis of pectin Ag NPs was optimized: 50 m L pectin solution(3 g/L) was prepared by Na OH(20 g/L), temperature 70 ℃, silver nitrate(1 m L, 0.5 mol/L) was dropped in the solution, reaction time 1 min. UV Vis spectra showed that the nanoparticles had a strong absorption at 410 nm, and transmission electron microscopy(TEM) showed that the average size of the prepared nanoparticles was 5-15 nm and had a narrow size distribution, the silver nanoparticles were spherical by scanning electron microscopy(SEM). This study will pave a useful way for the green synthesis process of Ag NPs, it will lay a theoretical foundation and provide a reference for antimicrobial materials development and utilization.
Ag nanoparticles(Ag NPs) have broad-spectrum antibacterial properties and low toxicity to mammalian cells, which have been widely recognized and applied. However, the preparation process of Ag NPs was tedious, and the chemical reagents used were easy to cause environmental pollution. So in this experiment, the green synthesis of Ag NPs was studied by using natural biological macromolecules citrus pectin as reducing agent and stabilizer. In this experiment,orange peel pectin was extracted by acid extract and ethanol precipitation, the yield of pectin was 24.2%, the content of glucuronic acid was 73.5%, and the esterification degree was 75.6%. The green synthesis of pectin Ag NPs was optimized: 50 m L pectin solution(3 g/L) was prepared by Na OH(20 g/L), temperature 70 ℃, silver nitrate(1 m L, 0.5 mol/L) was dropped in the solution, reaction time 1 min. UV Vis spectra showed that the nanoparticles had a strong absorption at 410 nm, and transmission electron microscopy(TEM) showed that the average size of the prepared nanoparticles was 5-15 nm and had a narrow size distribution, the silver nanoparticles were spherical by scanning electron microscopy(SEM). This study will pave a useful way for the green synthesis process of Ag NPs, it will lay a theoretical foundation and provide a reference for antimicrobial materials development and utilization.
引文
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[14]耿敬章,陈志远.橘皮果胶的提取工艺及性质研究[J].食品工业,2013,35(5):82-85.
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[16]李杰,臧晋,兰玉芳.柑橘皮中果胶的提取工艺研究[J].江西农业学报,2009,21(7):137-138.
[17]王小银,李精,殷军艺,等.新鲜与干制柑橘皮果胶物化特性比较分析[J].食品科学,2014,35(13):66-69.
[18]HEBEISH A A,EL-RAFIE M H,ABDEL-MOHDY F A,et al.Carboxymethyl cellulose for green synthesis and stabilization of silver nanoparticles[J].Carbohydrate Polymers,2010,82(3):933-941.
[19]MORONES J R,ELECHIGUERRA J L,CAMACHO A,et al.The bactericidal effect of silver nanoparticles[J].Nanotechnology,2005,16(10):2346-2353.
[2]TUMMALAPALLI M,DEOPURA B L,ALAM M S,et al.Facile and green synthesis of silver nanoparticles using oxidized pectin[J].Materials Science and Engineering:C,2015,50(5):31-36.
[3]MARAMBIO-JONES C,HOEK E V.A review of the antibacterial effects of silver nanomaterials and potential im-plications for human health and the environment[J].Journal of Nanoparticle Research,2010,12(5):1531-1551.
[4]MOHAN S,OLUWAFEMI O S,SONGCA S P,et al.Synthesis,antibacterial,cytotoxicity and sensing properties of starch-capped silver nanoparticles[J].Journal of Molecular Liquids,2016,213(1):75-81.
[5]THAKKAR K N,MHATRE S S,PARIKH R Y.Biological synthesis of metallic nanoparticles[J].Nanomedicine-Nan-otechnology Biology and Medicine,2010,6(2):257-262.
[6]ZAIN N M,STAPLEY A G F,SHAMA G.Green synthesis of silver and copper nanoparticles using ascorbic acid and chitosan for antimicrobial applications[J].Carbohydrate Polymers,2014,112(20):195-202.
[7]ZHOU Y,ZHAO Y,WANG L,et al.Radiation synthesis and characterization of nanosilver/gelatin/carboxymethyl chitosan hydrogel[J].Radiation Physics and Chemistry,2012,81(5):553-560.
[8]AHMED H B,ZAHRAN M K,EMAM H E.Heatless synthesis of well dispersible Au nanoparticles using pectin biopolymer[J].International Journal of Biological Macromolecules,2016,91(10):208-219.
[9]ZAHRAN M K,AHMED H B,EL-RAFIE M H.Facile size-regulated synthesis of silver nanoparticles using pectin[J].Carbohydrate Polymers,2014,111(1):971-978.
[10]BALACHANDRAN Y L,PANARIN A Y,KHODASEVICH I A,et al.Environmentally Friendly Preparation of Gold and Silver Nanoparticles for Sers Applications Using Biopolymer Pectin[J].Journal of Applied Spectroscopy,2015,81(6):962-968.
[11]NIGOGHOSSIAN K,DOS SANTOS M V,BARUD H S,et al.Orange pectin mediated growth and stability of aqueous gold and silver nanocolloids[J].Applied Surface Science,2015,341(18):28-36.
[12]VIRKUTYTE J,VARMA R S.Chem Inform abstract:green synthesis of metal nanoparticles:biodegradable polymers and enzymes in stabilization and surface functionalization[J].chemical Science,2011,42(34):837-846.
[13]李加兴,吴萍,吴越,等.八月瓜果皮果胶提取工艺优化及其理化特性研究[J].食品工业科技,2016,37(1):275-283.
[14]耿敬章,陈志远.橘皮果胶的提取工艺及性质研究[J].食品工业,2013,35(5):82-85.
[15]戴余军,石会军.酸解醇沉法提取柑橘皮果胶工艺的优化[J].北方园艺,2012,36(1):54-57.
[16]李杰,臧晋,兰玉芳.柑橘皮中果胶的提取工艺研究[J].江西农业学报,2009,21(7):137-138.
[17]王小银,李精,殷军艺,等.新鲜与干制柑橘皮果胶物化特性比较分析[J].食品科学,2014,35(13):66-69.
[18]HEBEISH A A,EL-RAFIE M H,ABDEL-MOHDY F A,et al.Carboxymethyl cellulose for green synthesis and stabilization of silver nanoparticles[J].Carbohydrate Polymers,2010,82(3):933-941.
[19]MORONES J R,ELECHIGUERRA J L,CAMACHO A,et al.The bactericidal effect of silver nanoparticles[J].Nanotechnology,2005,16(10):2346-2353.