预处理对水稻小孢子诱导愈伤组织产量和绿苗分化的影响
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摘要
为了提高水稻小孢子离体培养中愈伤组织诱导和再生效率,以4份水稻为材料,研究了温度、渗透压(甘露醇)和化学(秋水仙碱)等预处理离体花药和小孢子对愈伤产量和绿苗分化的影响。结果表明:水稻离体花药分别于60 g/L甘露醇和10 mg/L秋水仙碱中预处理3 d均能明显提高小孢子愈伤组织产量及绿苗产量;游离小孢子于32℃热击预处理2 d也能提高小孢子愈伤组织产量和绿苗产量。
In order to improve the efficiency of callus induction and regeneration in rice( Oryza sativa) microspore culture in vitro,the in vitro anther was pretreated with mannitol and colchicine at various temperatures.The results showed that the 3-d pretreatment of the in vitro anther with 60 g/L mannitol or 10 mg/L colchicine could obviously improve the callus yield and the green plantlet yield,and the 2-d 32 ℃ heat shock pretreatment of the free microspores could also increase both callus and green plantlet yields.
In order to improve the efficiency of callus induction and regeneration in rice( Oryza sativa) microspore culture in vitro,the in vitro anther was pretreated with mannitol and colchicine at various temperatures.The results showed that the 3-d pretreatment of the in vitro anther with 60 g/L mannitol or 10 mg/L colchicine could obviously improve the callus yield and the green plantlet yield,and the 2-d 32 ℃ heat shock pretreatment of the free microspores could also increase both callus and green plantlet yields.
引文
[1]王玲仙,蔺忠龙,白现广,等.水稻游离小孢子培养最新研究进展[J].生物技术,2009,19(6):92-95.
[2]韦鹏霄,岑秀芬,陈兆贵,等.水稻花药培养及育种应用研究[J].广西农业生物科学,1999,18(1):88-94.
[3]李艳萍.水稻花药培养与花培育种研究[J].天津农业科学,2003,9(4):36-39.
[4]Ferrie A M R,Caswell K L.Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production[J].Plant Cell Tissue Organ Culture,2011,104(3):301-309.
[5]相志国,海燕,康明辉,等.单倍体的产生途径及其在作物遗传育种中的应用[J].河南农业科学,2011,40(11):17-21.
[6]王宏芝.小麦游离小孢子培养研究进展[J].麦类作物学报,2002,22(1):91-94.
[7]Jahne A,Lorz H.Cereal microspore culture[J].Plant Science,1995,109(1):1-12.
[8]黄剑华.小孢子和花药培养技术在作物定向遗传改良上的应用[J].作物研究,2007(3):167-169.
[9]Touraev A,Vicente O,Heberle-Bors E.Initiation of microspore embryogenesis by stress[J].Trends in Plant Science,1997,2:297-3 0 2.
[10]Germana M A.Anther culture for haploid and doubled haploid production[J].Plant Cell Tiss Organ Cult.,2011,104:283-300.
[11]陆瑞菊,黄剑华,孙月芳,等.秋水仙碱对大麦离体培养小孢子存活与成苗的影响[J].植物生理学报,2001,27(2):135-140.
[12]Lu R J,Wang Y F,Sun Y F,et al.Improvement of isolated microspore culture of barley(Hordeum vulgare L.):the effect of floret co-culture[J].Plant Cell,Tissue and Organ Culture,2008,93(1):21-27.
[13]Prem D,Gupta K,Agnihotri A.Effect of various exogenous and endogenous factors on microspore embryogenesis in Indian mustard(Brassica juncea(L.)Czern and Coss)[J].In Vitro Cellular and Developmental Biology-Plant,2005,41(3):266-273.
[14]Santra M,Ankrah N,Santra D K,et al.An improved wheat microspore culture technique for the production of doubled haploid plants[J].Crop Science,2012,52(5):2314-2320.
[15]Andersen S B,Due I K,Olesen A.The response of anther culture in a genetically wide material of winter wheat(Triticum aestivum L.)[J].Plant Breeding,1987,99:181-186.
[16]Yuan S X,Liu Y M,Fang Z Y,et al.Effect of combined cold pretreatment and heat shock on microspore cultures in broccoli[J].Plant Breeding,2011,130(1):80-85.
[17]Ayed O S,Buyser J D,Picard E,et al.Effect of pre-treatment on isolated microspores culture ability in durum wheat(Triticum turgidum subsp.durum Desf.)[J].Journal of Plant Breeding and Crop Science,2010,2(2):30-38.
[18]Herath H M I,Bandara D C,Samarajeewa P K,et al.Effect of low temperature pre-treatment on anther culture in selected indica,japonica rice varieties and their inter sub-specific hybrids[J].Cey.J.Sci.(Bio.Sci.),2009,38(1):11-16.
[19]Redha A,Talaat A.Improvement of green plant regeneration by manipulation of anther culture induction medium of hexaploid wheat[J].Plant Cell,Tissue and Organ Culture,2008,92(2):141-146.
[20]Zheng M Y.Microspore culture in wheat(Triticum aestivum)-doubled haploid production via induced embryogenesis[J].Plant Cell,Tissue and Culture,2003,73(3):213-230.
[21]Islam S M S.The effect ofcolchicine pretreatment on isolated microspore culture of wheat(Triticum aestivum L.)[J].Australian Journal of Crop Science,2010,4(9):660-665.
[22]李文泽,景健康,姚根怀,等.基因型和甘露醇预处理对大麦花粉植株高频率再生的影响[J].科学通报,1992,37(10):935-938.
[23]陆瑞菊,孙月芳,王亦菲,等.提高大麦游离小孢子培养反应的研究[J].大麦科学,2004(2):14-16.
[24]Bal U,Abak K,Buyukalaca S,et al.Development of callus colonies from the isolated microspore culture of Capsicum annuum L.[J].Biotechnology and Biotechnological Equipment,2003,17(2):38-43.
[25]Ouyang J W,Zhou S M,Jia S E.The response of anther culture to culture temperature in Triticum aestivum[J].Theoretical and Applied Genetics,1983,66(2):101-109.
[26]Chuong P V,Beversdorf W D.High frequency embryogenesis through isolated microspore culture in Brassica napus L.and B.Carinata braun[J].Plant Science,1985,39:219-226.
[2]韦鹏霄,岑秀芬,陈兆贵,等.水稻花药培养及育种应用研究[J].广西农业生物科学,1999,18(1):88-94.
[3]李艳萍.水稻花药培养与花培育种研究[J].天津农业科学,2003,9(4):36-39.
[4]Ferrie A M R,Caswell K L.Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production[J].Plant Cell Tissue Organ Culture,2011,104(3):301-309.
[5]相志国,海燕,康明辉,等.单倍体的产生途径及其在作物遗传育种中的应用[J].河南农业科学,2011,40(11):17-21.
[6]王宏芝.小麦游离小孢子培养研究进展[J].麦类作物学报,2002,22(1):91-94.
[7]Jahne A,Lorz H.Cereal microspore culture[J].Plant Science,1995,109(1):1-12.
[8]黄剑华.小孢子和花药培养技术在作物定向遗传改良上的应用[J].作物研究,2007(3):167-169.
[9]Touraev A,Vicente O,Heberle-Bors E.Initiation of microspore embryogenesis by stress[J].Trends in Plant Science,1997,2:297-3 0 2.
[10]Germana M A.Anther culture for haploid and doubled haploid production[J].Plant Cell Tiss Organ Cult.,2011,104:283-300.
[11]陆瑞菊,黄剑华,孙月芳,等.秋水仙碱对大麦离体培养小孢子存活与成苗的影响[J].植物生理学报,2001,27(2):135-140.
[12]Lu R J,Wang Y F,Sun Y F,et al.Improvement of isolated microspore culture of barley(Hordeum vulgare L.):the effect of floret co-culture[J].Plant Cell,Tissue and Organ Culture,2008,93(1):21-27.
[13]Prem D,Gupta K,Agnihotri A.Effect of various exogenous and endogenous factors on microspore embryogenesis in Indian mustard(Brassica juncea(L.)Czern and Coss)[J].In Vitro Cellular and Developmental Biology-Plant,2005,41(3):266-273.
[14]Santra M,Ankrah N,Santra D K,et al.An improved wheat microspore culture technique for the production of doubled haploid plants[J].Crop Science,2012,52(5):2314-2320.
[15]Andersen S B,Due I K,Olesen A.The response of anther culture in a genetically wide material of winter wheat(Triticum aestivum L.)[J].Plant Breeding,1987,99:181-186.
[16]Yuan S X,Liu Y M,Fang Z Y,et al.Effect of combined cold pretreatment and heat shock on microspore cultures in broccoli[J].Plant Breeding,2011,130(1):80-85.
[17]Ayed O S,Buyser J D,Picard E,et al.Effect of pre-treatment on isolated microspores culture ability in durum wheat(Triticum turgidum subsp.durum Desf.)[J].Journal of Plant Breeding and Crop Science,2010,2(2):30-38.
[18]Herath H M I,Bandara D C,Samarajeewa P K,et al.Effect of low temperature pre-treatment on anther culture in selected indica,japonica rice varieties and their inter sub-specific hybrids[J].Cey.J.Sci.(Bio.Sci.),2009,38(1):11-16.
[19]Redha A,Talaat A.Improvement of green plant regeneration by manipulation of anther culture induction medium of hexaploid wheat[J].Plant Cell,Tissue and Organ Culture,2008,92(2):141-146.
[20]Zheng M Y.Microspore culture in wheat(Triticum aestivum)-doubled haploid production via induced embryogenesis[J].Plant Cell,Tissue and Culture,2003,73(3):213-230.
[21]Islam S M S.The effect ofcolchicine pretreatment on isolated microspore culture of wheat(Triticum aestivum L.)[J].Australian Journal of Crop Science,2010,4(9):660-665.
[22]李文泽,景健康,姚根怀,等.基因型和甘露醇预处理对大麦花粉植株高频率再生的影响[J].科学通报,1992,37(10):935-938.
[23]陆瑞菊,孙月芳,王亦菲,等.提高大麦游离小孢子培养反应的研究[J].大麦科学,2004(2):14-16.
[24]Bal U,Abak K,Buyukalaca S,et al.Development of callus colonies from the isolated microspore culture of Capsicum annuum L.[J].Biotechnology and Biotechnological Equipment,2003,17(2):38-43.
[25]Ouyang J W,Zhou S M,Jia S E.The response of anther culture to culture temperature in Triticum aestivum[J].Theoretical and Applied Genetics,1983,66(2):101-109.
[26]Chuong P V,Beversdorf W D.High frequency embryogenesis through isolated microspore culture in Brassica napus L.and B.Carinata braun[J].Plant Science,1985,39:219-226.