预处理和培养温度对陆稻游离小孢子愈伤组织诱导及分化的影响
|
摘要
以陆稻品种‘云2号’和‘云3号’为材料,分别研究了花药预处理、游离小孢子预处理和小孢子初期培养温度对小孢子愈伤组织产量及绿苗形成数的影响。结果表明,将游离前的小孢子花药在32℃下预处理2 d,或将游离小孢子在32℃下预处理2 d,均可以提高小孢子的愈伤组织产量;将游离小孢子置于32℃培养2 d后转入25℃培养优于只置于25℃培养,可以明显提高小孢子愈伤组织产量及绿苗形成数量。
Two cultivars of upland rice were used to investigate the effects of pretreatments of anther or isolated microspores and the initial culture temperature on callus yield and number of green plantlets. The results showed that 2 d of 32 ℃ pretreatment on anthers or isolated microspores could improve the callus yield. Comparing to continuous culture under 25 ℃, 2 d initial culture under 32 ℃ prior to subculture under 25 ℃ could remarkably improve the yields of the callus and green plantlet.
Two cultivars of upland rice were used to investigate the effects of pretreatments of anther or isolated microspores and the initial culture temperature on callus yield and number of green plantlets. The results showed that 2 d of 32 ℃ pretreatment on anthers or isolated microspores could improve the callus yield. Comparing to continuous culture under 25 ℃, 2 d initial culture under 32 ℃ prior to subculture under 25 ℃ could remarkably improve the yields of the callus and green plantlet.
引文
边立娜,牛国保,孙德岭(2013).花椰菜游离小孢子培养研究进展天津农业科学,19(6):5~7
曹丽娟,栾非时,李锡香(2005).植物游离小孢子培养的研究进展东北农业大学学报,36(5):660~663
翟伟,胡小荣,周红立,陶梅(2010).旱稻的抗旱性及遗传改良研究现状.植物遗传资源学报,11(4):394~398
董静,邬飞波,张国平(2005).麦类作物小孢子培养研究进展.麦类作物学报,25(2):102~106
顾祥昆,李菲,张淑江,章时蕃,张慧,孙日飞(2013).芥菜游离小孢子培养技术研究.中国蔬菜,(12):23~30
贺梅,黄少锋,张丽萍,宋冬明(2010).花药培养育种在水稻育种上的应用.北方水稻,40(1):75~78
胡颂平,周清明(2001).陆稻抗旱性研究进展.湖南农业大学学报(自然科学版),27(3):240~244
黄剑华(2007).小孢子和花药培养技术在作物定向遗传改良上的应用.作物研究,(3):167~169
姜健,金成海,侯春香,金信忍,杨宝灵(2001).水稻花药培养研究与应用进展.中国农学通报,17(4):49~52
金航,张金渝,张智慧(2006).影响花药培养的主要因素.现代中药研究与实践,20(3):61~64
李光威,兰素缺,孙宝启(2001).小麦游离小孢子培养的研究.华北农学报,16(1):83~87
梁辉,欧阳俊闻,贾双娥,张弛,贾旭(1997).小麦花药培养初期高温处理对培养反应的影响.科学通报,42(13):1436~1439
柳美南,钟海明,陈绵桥,姚志坚,黄蓉芬,唐娟,刘海林(2008).水稻花药培养及其应用.现代农业科技,(17):234,236
陆瑞菊,黄剑华,何南扬,龚来庭,王亦菲,孙月芳,周润梅(2002).应用小孢子离体培养技术培育大麦新品系.麦类作物学报,22(4):88~90
陆瑞菊,黄剑华,孙月芳,王亦菲,周润梅(2001).秋水仙碱对大麦离体培养小孢子存活与成苗的影响.植物生理学报,27(2):135~140
牛永超,李加纳,殷家明,林呐,徐新福,韩甫,周清元(2010).基因型和处理温度对芥菜型油菜游离小孢子培养的影响.西南大学学报(自然科学版),32(8):23~28
秦余香,夏光敏(2004).小麦的小孢子培养.植物学通报,21(5):625~630
王玲仙,蔺忠龙,白现广,吕广磊,付坚,殷富有,黄兴奇,程在全(2009).水稻游离小孢子培养最新研究进展.生物技术,19(6):92~95
于凤池,姚坚,姚海根(2009).水稻花药培养的影响因素及其发展探析.现代农业科技,(15):59,62
张跃非,李碧如(2010).温度在水稻花药培养过程中的影响研究.吉林农业,(11):68,76
张跃非,王金玲,邓晓容(2010).高温热击处理对培养水稻花药愈伤组织形成的影响.绵阳师范学院学报,29(11):81~84
赵爱菊,高增玉,李亚军,刘玉平,陈希勇(2008).禾谷类作物的小孢子培养.河北农业科学,12(4):73~74,80
周伟军,毛碧增,顾宏辉,唐桂香(2002).秋水仙碱及热击与低温诱导对油菜小孢子胚状体成苗率的影响.作物学报,28(3):369~373
庄军平,巩振辉,苏菁(2001).温度对辣椒花药愈伤组织形成的影响.西北农业学报,10(2):49~51
Datta SK,Datta K,Potrykus I(1990).Embryogenesis and plant regeneration from microspores of both‘indica’and‘japonica’rice(Oryza sativa).Plant Sci,67:83~88
Germana MA(2011).Anther culture for haploid and doubled haploidproduction.Plant Cell Tiss Org,104:283~300
Islam SMS(2010).The effect of colchicine pretreatment on isolatedmicrospore culture of wheat(Triticum aestivum L.).Aust J CropSci,4(9):660~665
Lu RJ,Wang YF,Sun YF,Shan LL,Chen PD,Huang JH(2008).Improvement of isolated microspore culture of barley(Hordeumvulgare L.):the effect of floret co-culture.Plant Cell Tiss Org,93(1):21~27
Oleszczuk S,Sowa S,Zimny J(2006).Androgenic response to preculture stress in microspore cultures of barley.Protoplasma,228:95~100
Pechan PM,Bartels D,Brown DCW,Schell J(1991).Messenger-RNA and protein changes associated with induction of Brassica microspore embryogenesis.Planta,184:161~165
Prem D,Gupta K,Agnihotri A(2005).Effect of various exogenousand endogenous factors on microspore embryogenesis in Indianmustard(Brassica juncea L.Czern and Coss).In Vitro Cell DevBiol-Pl,41(3):266~273
Redha A,Talaat A(2008).Improvement of green plant regenerationby manipulation of anther culture induction medium of hexaploid wheat.Plant Cell Tiss Org,92(2):141~146
Santra M,Ankrah N,Santra DK,Kidwell KK(2012).An improvedwheat microspore culture technique for the production of doubled haploid plants.Crop Sci,52(5):2314~2320
Shariatpanahi ME,Bal U,Heberle-Bors E,Touraev A(2006).Stressesapplied for the re-programming of plant microspores towards invitro embryogenesis.Physiol Plant,127:519~534
Touraev A,Indrianto A,Wratschko I,Vicente O,Heberle-Bors E(1996).Efficient microspore embryogenesis in wheat(Triticumaestivum L.)induced by starvation at high temperature.SexPlant Rep,9:209~215
Yuan SX,Liu YM,Fang ZY,Yang LM,Zhuang M,Zhang YY,Sun PT(2011).Effect of combined cold pretreatment and heat shock onmicrospore cultures in broccoli.Plant Breeding,130(1):80~85
Zhao JP,Simmonds D,Newcomb W(1996).Induction of embryogenesis with colchicines instead of heat in microspores of Brassicanapus L.cv.Topas.Planta,198:433~439
曹丽娟,栾非时,李锡香(2005).植物游离小孢子培养的研究进展东北农业大学学报,36(5):660~663
翟伟,胡小荣,周红立,陶梅(2010).旱稻的抗旱性及遗传改良研究现状.植物遗传资源学报,11(4):394~398
董静,邬飞波,张国平(2005).麦类作物小孢子培养研究进展.麦类作物学报,25(2):102~106
顾祥昆,李菲,张淑江,章时蕃,张慧,孙日飞(2013).芥菜游离小孢子培养技术研究.中国蔬菜,(12):23~30
贺梅,黄少锋,张丽萍,宋冬明(2010).花药培养育种在水稻育种上的应用.北方水稻,40(1):75~78
胡颂平,周清明(2001).陆稻抗旱性研究进展.湖南农业大学学报(自然科学版),27(3):240~244
黄剑华(2007).小孢子和花药培养技术在作物定向遗传改良上的应用.作物研究,(3):167~169
姜健,金成海,侯春香,金信忍,杨宝灵(2001).水稻花药培养研究与应用进展.中国农学通报,17(4):49~52
金航,张金渝,张智慧(2006).影响花药培养的主要因素.现代中药研究与实践,20(3):61~64
李光威,兰素缺,孙宝启(2001).小麦游离小孢子培养的研究.华北农学报,16(1):83~87
梁辉,欧阳俊闻,贾双娥,张弛,贾旭(1997).小麦花药培养初期高温处理对培养反应的影响.科学通报,42(13):1436~1439
柳美南,钟海明,陈绵桥,姚志坚,黄蓉芬,唐娟,刘海林(2008).水稻花药培养及其应用.现代农业科技,(17):234,236
陆瑞菊,黄剑华,何南扬,龚来庭,王亦菲,孙月芳,周润梅(2002).应用小孢子离体培养技术培育大麦新品系.麦类作物学报,22(4):88~90
陆瑞菊,黄剑华,孙月芳,王亦菲,周润梅(2001).秋水仙碱对大麦离体培养小孢子存活与成苗的影响.植物生理学报,27(2):135~140
牛永超,李加纳,殷家明,林呐,徐新福,韩甫,周清元(2010).基因型和处理温度对芥菜型油菜游离小孢子培养的影响.西南大学学报(自然科学版),32(8):23~28
秦余香,夏光敏(2004).小麦的小孢子培养.植物学通报,21(5):625~630
王玲仙,蔺忠龙,白现广,吕广磊,付坚,殷富有,黄兴奇,程在全(2009).水稻游离小孢子培养最新研究进展.生物技术,19(6):92~95
于凤池,姚坚,姚海根(2009).水稻花药培养的影响因素及其发展探析.现代农业科技,(15):59,62
张跃非,李碧如(2010).温度在水稻花药培养过程中的影响研究.吉林农业,(11):68,76
张跃非,王金玲,邓晓容(2010).高温热击处理对培养水稻花药愈伤组织形成的影响.绵阳师范学院学报,29(11):81~84
赵爱菊,高增玉,李亚军,刘玉平,陈希勇(2008).禾谷类作物的小孢子培养.河北农业科学,12(4):73~74,80
周伟军,毛碧增,顾宏辉,唐桂香(2002).秋水仙碱及热击与低温诱导对油菜小孢子胚状体成苗率的影响.作物学报,28(3):369~373
庄军平,巩振辉,苏菁(2001).温度对辣椒花药愈伤组织形成的影响.西北农业学报,10(2):49~51
Datta SK,Datta K,Potrykus I(1990).Embryogenesis and plant regeneration from microspores of both‘indica’and‘japonica’rice(Oryza sativa).Plant Sci,67:83~88
Germana MA(2011).Anther culture for haploid and doubled haploidproduction.Plant Cell Tiss Org,104:283~300
Islam SMS(2010).The effect of colchicine pretreatment on isolatedmicrospore culture of wheat(Triticum aestivum L.).Aust J CropSci,4(9):660~665
Lu RJ,Wang YF,Sun YF,Shan LL,Chen PD,Huang JH(2008).Improvement of isolated microspore culture of barley(Hordeumvulgare L.):the effect of floret co-culture.Plant Cell Tiss Org,93(1):21~27
Oleszczuk S,Sowa S,Zimny J(2006).Androgenic response to preculture stress in microspore cultures of barley.Protoplasma,228:95~100
Pechan PM,Bartels D,Brown DCW,Schell J(1991).Messenger-RNA and protein changes associated with induction of Brassica microspore embryogenesis.Planta,184:161~165
Prem D,Gupta K,Agnihotri A(2005).Effect of various exogenousand endogenous factors on microspore embryogenesis in Indianmustard(Brassica juncea L.Czern and Coss).In Vitro Cell DevBiol-Pl,41(3):266~273
Redha A,Talaat A(2008).Improvement of green plant regenerationby manipulation of anther culture induction medium of hexaploid wheat.Plant Cell Tiss Org,92(2):141~146
Santra M,Ankrah N,Santra DK,Kidwell KK(2012).An improvedwheat microspore culture technique for the production of doubled haploid plants.Crop Sci,52(5):2314~2320
Shariatpanahi ME,Bal U,Heberle-Bors E,Touraev A(2006).Stressesapplied for the re-programming of plant microspores towards invitro embryogenesis.Physiol Plant,127:519~534
Touraev A,Indrianto A,Wratschko I,Vicente O,Heberle-Bors E(1996).Efficient microspore embryogenesis in wheat(Triticumaestivum L.)induced by starvation at high temperature.SexPlant Rep,9:209~215
Yuan SX,Liu YM,Fang ZY,Yang LM,Zhuang M,Zhang YY,Sun PT(2011).Effect of combined cold pretreatment and heat shock onmicrospore cultures in broccoli.Plant Breeding,130(1):80~85
Zhao JP,Simmonds D,Newcomb W(1996).Induction of embryogenesis with colchicines instead of heat in microspores of Brassicanapus L.cv.Topas.Planta,198:433~439