绿豆皮黄酮的纯化工艺研究
|
摘要
以绿豆皮为原料,主要研究了绿豆皮黄酮的纯化工艺。首先比较了AB-8,LSA-10,HPD-100和ADS-7型4种大孔树脂对绿豆皮黄酮的吸附和解析特性,选择其中最好的树脂,对其吸附和解析条件进行了研究。结果表明,AB-8型是纯化绿豆皮黄酮较好的大孔树脂,其最佳吸附条件为上样速率1.98 m L/min,上样质量浓度0.045 9 mg/m L,上样量10 m L时,最大吸附率可达88.89%;其最佳解析条件为解析液乙醇体积分数70%,解析液洗脱流速1.98 m L/min,解析液用量25 m L时,解析率可达69.51%。绿豆皮黄酮的纯度由10.57%提高至72.19%,表明该纯化工艺具有较好的纯化效果,可为绿豆皮黄酮的纯化及进一步开发应用提供参考。
In this paper,the purification technology of flavonoids from Mung tegument was studied. Firstly,the adsorption and desorption properties of macroporous resins such as AB-8,LSA-10,HPD-100 and ADS-7 were compared. The preferred resin was screened and the adsorption and analytical conditions were studied. The results showed that AB-8 was the best macroporous resin with the best flavonoids. The best adsorption conditions were as follows:the loading rate was 1.98 m L/min,adsorption liquid concentration of 0.045 9 mg/m L,the upper sample was 10 m L,the maximum adsorption rate could up to88.89%. Its best analytical conditions:analytical liquid concentration of 70%,the flow rate was 1.98 m L/min,and analytical liquid dosage was 25 m L,the analytical rate was 69.51%. The purity of flavonoids in the extract was increased from 10.57% to72.19%,which indicated that the purification process had better purification effect,which can provide reference for the purification and further development and application of flavonoids from Mung tegument.
In this paper,the purification technology of flavonoids from Mung tegument was studied. Firstly,the adsorption and desorption properties of macroporous resins such as AB-8,LSA-10,HPD-100 and ADS-7 were compared. The preferred resin was screened and the adsorption and analytical conditions were studied. The results showed that AB-8 was the best macroporous resin with the best flavonoids. The best adsorption conditions were as follows:the loading rate was 1.98 m L/min,adsorption liquid concentration of 0.045 9 mg/m L,the upper sample was 10 m L,the maximum adsorption rate could up to88.89%. Its best analytical conditions:analytical liquid concentration of 70%,the flow rate was 1.98 m L/min,and analytical liquid dosage was 25 m L,the analytical rate was 69.51%. The purity of flavonoids in the extract was increased from 10.57% to72.19%,which indicated that the purification process had better purification effect,which can provide reference for the purification and further development and application of flavonoids from Mung tegument.
引文
[1]曹维强,王静.绿豆综合开发及利用[J].粮食与油脂,2003,17(3):37-39.
[2]Furao L,Qibiao W,Lin L,et al.Antioxidant activities of water-soluble polysaccharide extracted from mung bean(Vigna radiata L).hull with ultrasonic assisted treatment[J].Carbohydrate Polymers,2010(2):323-329.
[3]邓志汇,王娟.绿豆皮与绿豆仁的营养成分分析及对比[J].现代食品科技,2010,26(6):656-659.
[4]裴凌鹏,惠伯棣,金宗濂,等.黄酮类化合物的生理活性及其制备技术研究进展[J].食品科学,2004,25(2):203-207.
[5]汪少芸,叶秀云,饶平凡.绿豆生物活性物质及功能的研究进展[J].中国食品学报,2004(1):98-102.
[6]吕萍,刘景圣,蔡丹,等.超声波法提取绿豆中的黄酮的最佳工艺研究[J].粮油加工,2010,12(8):117-119.
[7]任顺成,定霄霖.大孔树脂对玉米须类黄酮的吸附分离特性的研究[J].食品与发酵工业,2003,29(12):17-20.
[8]胡志军,郝利君,王南溪.D-101大孔吸附树脂分离纯化橘皮中的黄酮类物质[J].食品科学,2010,31(8):65-69.
[9]吴海霞,吴彩娥,李婷婷,等.大孔树脂纯化银杏叶黄酮的研究[J].现代食品科技,2013,29(12):2 964-2 969.
[10]朱宏莉,韦海洪,宋纪蓉,等.竹叶总黄酮的提取与纯化工艺研究[J].食品科学,2005,26(8):158-161.
[11]陈婷婷,徐娟,赵珺.绿豆皮中黄酮类化合物提取工艺[J].生物加工过程,2008,6(1):60-64.
[12]苗修港,余翔,张贝贝,等.NKA-9大孔树脂纯化香椿叶黄酮类物质工艺优化[J].食品科学,2016,37(8):1-12.
[13]董文宾,徐颖,张建华,等.LSA-10型树脂纯化大豆异黄酮工艺条件的研究[J].粮油加工与食品机械,2004(7):43-45.
[2]Furao L,Qibiao W,Lin L,et al.Antioxidant activities of water-soluble polysaccharide extracted from mung bean(Vigna radiata L).hull with ultrasonic assisted treatment[J].Carbohydrate Polymers,2010(2):323-329.
[3]邓志汇,王娟.绿豆皮与绿豆仁的营养成分分析及对比[J].现代食品科技,2010,26(6):656-659.
[4]裴凌鹏,惠伯棣,金宗濂,等.黄酮类化合物的生理活性及其制备技术研究进展[J].食品科学,2004,25(2):203-207.
[5]汪少芸,叶秀云,饶平凡.绿豆生物活性物质及功能的研究进展[J].中国食品学报,2004(1):98-102.
[6]吕萍,刘景圣,蔡丹,等.超声波法提取绿豆中的黄酮的最佳工艺研究[J].粮油加工,2010,12(8):117-119.
[7]任顺成,定霄霖.大孔树脂对玉米须类黄酮的吸附分离特性的研究[J].食品与发酵工业,2003,29(12):17-20.
[8]胡志军,郝利君,王南溪.D-101大孔吸附树脂分离纯化橘皮中的黄酮类物质[J].食品科学,2010,31(8):65-69.
[9]吴海霞,吴彩娥,李婷婷,等.大孔树脂纯化银杏叶黄酮的研究[J].现代食品科技,2013,29(12):2 964-2 969.
[10]朱宏莉,韦海洪,宋纪蓉,等.竹叶总黄酮的提取与纯化工艺研究[J].食品科学,2005,26(8):158-161.
[11]陈婷婷,徐娟,赵珺.绿豆皮中黄酮类化合物提取工艺[J].生物加工过程,2008,6(1):60-64.
[12]苗修港,余翔,张贝贝,等.NKA-9大孔树脂纯化香椿叶黄酮类物质工艺优化[J].食品科学,2016,37(8):1-12.
[13]董文宾,徐颖,张建华,等.LSA-10型树脂纯化大豆异黄酮工艺条件的研究[J].粮油加工与食品机械,2004(7):43-45.