动物源性食品安全检测技术研究进展
|
摘要
食品安全问题受到全球关注,动物源性食品安全问题屡遭曝光。经过加工重组的动物源性食品,其物种的原有形态已被破坏,感官鉴定很难辨别真伪。因此,本文详细论述了动物源性食品的定义、分类、掺假类型,说明了掺假的潜在危害,主要总结了动物性食品安全检测技术的研究进展,并对动物源性食品在中国的发展进行了展望,以期为动物源性食品安全检测研究提供参考。
Food safety is a critical,global concern,especially animal derived food safety issues. After processing and reorganizing,the original form of animal derived food has been destroyed,and sensory identification is hard to distinguish true from false.Therefore,this article discussed the definition,classification and adulteration of animal-derived foods in detail,explained the potential hazards of adulteration,summarized the detection techniques for animal derived food safety,and prospected the development of animal-derived foods in China in order to provide references for animal derived food safety detection.
Food safety is a critical,global concern,especially animal derived food safety issues. After processing and reorganizing,the original form of animal derived food has been destroyed,and sensory identification is hard to distinguish true from false.Therefore,this article discussed the definition,classification and adulteration of animal-derived foods in detail,explained the potential hazards of adulteration,summarized the detection techniques for animal derived food safety,and prospected the development of animal-derived foods in China in order to provide references for animal derived food safety detection.
引文
[1]Ballin N Z.Authentication of meat and meat products[J].Meat Science,2010,86(3):577-587.
[2]Sidel J L,Stone H.The role of sensory evaluation in the food industry[J].Food Quality and Preference,1993,4(1-2):65-73.
[3]Day L,Brown H.Detection of mechanically recovered chicken meat using capillary gel electrophoresis[J]. Meat Science,2001,58(1):31-37.
[4]Vallejo-Cordoba B,Rodríguez-Ramírez R,GonzálezCórdova A F.Capillary electrophoresis for bovine and ostrich meat characterisation[J].Food Chemistry,2010,120(1):304-307.
[5]阿力木·吾布力,杨洁,苏力坦·阿巴百克力. SDS-PAGE电泳在鉴别用牛乳掺伪的新疆特种乳中的应用[J].安徽农业科学,2016,44(23):99-102.
[6]侯艳霞,古丽巴哈尔·卡吾力,高晓黎.SDS-PAGE电泳法对4种鲜奶蛋白质组分的分析研究[J].新疆医科大学学报,2017,40(5):646-650.
[7]张婷,史晋绒,赵田田,等.四种鱼的血清、体表黏液SDSPAGE分析[J].四川动物,2015,34(6):880-884.
[8]孙克江,张甜,曲梅丽,等.聚丙烯酰胺凝胶电泳法快速检测植物源性食品中的蛋白成分[J].食品安全质量检测学报,2017,8(3):895-899.
[9]杨萧帆.蛋白电泳在小麦种子真实性及纯度鉴定中的应用[J].乡村科技,2017(21):63-66.
[10]刘姝,王凯民.肉类食品中猪源性成分检测方法之探讨[J].工程技术:全文版,2016(5):243-244.
[11] Giovannacci I,Guizard C,Carlier M,et al. Species identification of meat products by ELISA[J].International Journal of Food Science and Technology,2010,39(8):863-867.
[12]Hsieh M K,Shih P Y,Wei C F,et al.Detection of undeclared animal by-products in commercial canine canned foods:Comparative analyses by ELISA and PCR-RFLP coupled with slab gel electrophoresis or capillary gel electrophoresis[J].Journal of the Science of Food and Agriculture,2016,96(5):1659-1665.
[13]Li C,Zhang Y,Eremin S A,et al.Detection of kanamycin and gentamicin residues in animal-derived food using Ig Y antibody based ic-ELISA and FPIA[J]. Food Chemistry,2017,227:48-54.
[14]Chen X,Li Z,Guo J,et al. Simultaneous screening for marbofloxacin and ofloxacin residues in animal-derived foods using an indirect competitive immunoassay[J]. Food and Agricultural Immunology,2017,28(3):489-499.
[15]杨典原.氟苯尼考残留酶免疫法的研究[D].太原:山西大学,2016.
[16]赵秋伶,史素青,张尤华,等.酶联适体分析法检测动物源性食品中的卡那霉素残留[J].食品工业科技,2015,36(10):86-89.
[17]Claeys Erik,Vossen Els,De Smet Stefaan.Determination ofα-tocopherol by reversed-phase HPLC in feed and animalderived foods without saponification[J]. Journal of the Science of Food and Agriculture,2016,96(2):522-529.
[18]Zhu H,Yang S,Zhang Y,et al. Simultaneous detection of fifteen biogenic amines in animal derived products by HPLC-FLD with solid-phase extraction after derivatization with dansyl chloride[J].Analytical Methods,2016,8(18):3747-3755.
[19]池永红,云雅光.SPE-HPLC检测动物源性食品中3种氟喹诺酮类药物残留[J].食品研究与开发,2015(20):152-155.
[20]杨朝琳.高效液相色谱-串联质谱法检测动物源性食品中的抗生素[D].金华:浙江师范大学,2016.
[21]郝杰,姜洁,余建龙,等.固相萃取-超高效液相色谱-串联质谱法同时测定动物源性食品中多种兽药残留[J].食品科学,2017,38(12):266-272.
[22]Fei G,Zhou S,Han L,et al.A novel FT-IR spectroscopic method based on lipid characteristics for qualitative and quantitative analysis of animal-derived feedstuff adulterated with ruminant ingredients[J].Food Chemistry,2017,237:342.
[23]Gao F,Han L,Yang Z,et al.Technical note:Characterization of lipid constitution in Fourier transform infrared spectra and spectroscopic discrimination of animal-derived feedstuffs fromdifferent species[J]. Journal of Animal Science,2017,95(6):2794.
[24]吕程序,杨增玲,韩鲁佳,等.饲料中动物源性成分显微近红外光谱检测分析[J].农业机械学报,2011,42(5):171-174.
[25]Chikuni K,Ozutsumi K,Koishikawa T,et al. Species identification of cooked meats by DNA hybridization assay[J].Meat Science,1990,27(2):119-128.
[26]朱业培,王玮,吕青骎,等.基于基因芯片技术检测6种动物源性成分[J].南京农业大学学报,2015,38(6):1003-1008.
[27]石丰运,缪建锟,张利平,等.运用基因芯片技术检测牛、山羊、猪和鸡源性成分[J].生物工程学报,2010,26(6):823-829.
[28]王振全.基因芯片方法检测六种动物源性人兽共患病病原[D].长春:吉林大学,2012.
[29]Vences,M,Thomas,M,Bonett,RM,et al. Deciphering amphibian diversity through DNA barcoding:chances and challenges[J]. Philosophical Transactions of the Royal Society of London,2005,360(1462):1859-1868.
[30]Holmes B H,Steinke D,Ward R D. Identification of shark and ray fins using DNA barcoding[J].Fisheries Research,2009,95(2):280-288.
[31]Hellberg R S,Kawalek M D,Van K T,et al.Comparison of DNA extraction and PCR setup methods for use in highthroughput DNA barcoding of fish species[J]. Food Analytical Methods,2014,7(10):1950-1959.
[32]Shuang W,Yong-Bo L I,Chao-Feng M A,et al.Cytochrome C oxidase subunit I sequence as a DNA barcode to identify common meat species[J].Modern Food Science and Technology,2016,32(1):188-193.
[33]Wang M Y,Zhao Q Z,Liu B,et al.Species authority of fish and its derived products in Shanghai by DNA barcoding[J].Science and Technology of Food Industry,2016.
[34]Song T P,Yang L X,Qiu H L,et al. Loop-mediated isothermal amplification assay for identification of animal derived materials[J].Journal of Food Safety and Quality,2017.
[35]杨丽霞,付淑君,彭新凯.环介导等温扩增法检测牛羊肉中的猪肉成分[J].食品与机械,2013(5):63-65.
[36]李向丽,刘垚,谭贵良,等.基于LAMP法快速检测羊肉及其制品中的猪、鸭和羊源性成分[J].中国食品卫生杂志,2015,27(3):247-252.
[37]唐小春,卢新,冉光耀,等.一种基于lamp方法快速检测肉类掺假的试剂盒:中国,104404145 B[P].2015.
[38]李莎,张如胜,陈静芳.肠道病毒71型RT-LAMP快速检测方法的建立及初步应用[J].国际检验医学杂志,2017,38(4):470-472.
[39]陈希文,汪谦,尹苗,等.猪繁殖与呼吸综合征病毒RTLAMP快速检测方法的建立[J].中国畜牧兽医,2017,44(6):1630-1636.
[40]刘星星,耿毅,孙娜,等.兔出血症病毒可视化RT-LAMP检测方法的建立与应用[J].中国兽医科学,2017(6):687-693.
[41]熊炜,王艳,魏晓锋,等.可视化猪肺炎支原体LAMP检测方法的建立[J].中国动物传染病学报,2017,25(4):29-33.
[42]吕二盼.动物源性食品中各种动物源性肉及肉制品鉴别检验的研究[D].保定:河北农业大学,2012.
[43]Shinoda N,Kusama T,Yoshida T,et al. Developing PCR primers using a new computer program for detection of multiple animal-derived materials in feed[J].Journal of Food Protection,2008,71(11):2257.
[44]Zeng S,Qin Z,Ruan Z,et al.Multiplex fluorescent real-time PCR detection of bovine,goat and sheep derived materials in animal products[J]. Chinese Journal of Biotechnology,2009,25(1):139-146.
[45]Ali M E,Razzak M A,Hamid S B,et al.Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods[J].Food Chemistry,2015,177:214-224.
[46]Hou B,Meng X,Zhang L,et al.Development of a sensitive and specific multiplex PCR method for the simultaneous detection of chicken,duck and goose DNA in meat products[J]. Meat Science,2015,101:90-94.
[47]Tao T,Chen Q,Bie X,et al.Investigation on prevalence of Listeria,spp.and Listeria monocytogenes,in animal-derived foods by multiplex PCR assay targeting novel genes[J]. Food Control,2016,26(9):704-711.
[48]Xu R,Wei S,Zhou G,et al.Multiplex Taq Man locked nucleic acid real-time PCR for the differential identification of various meat and meat products[J].Meat Science,2017,137:41.
[49]Chen X T,Zhen hua K E.Research on multiplex RT-PCR detection method of animal and plant derived ingredients in goat milk[J]. Food Research and Development,2017,38(15):156-162.
[2]Sidel J L,Stone H.The role of sensory evaluation in the food industry[J].Food Quality and Preference,1993,4(1-2):65-73.
[3]Day L,Brown H.Detection of mechanically recovered chicken meat using capillary gel electrophoresis[J]. Meat Science,2001,58(1):31-37.
[4]Vallejo-Cordoba B,Rodríguez-Ramírez R,GonzálezCórdova A F.Capillary electrophoresis for bovine and ostrich meat characterisation[J].Food Chemistry,2010,120(1):304-307.
[5]阿力木·吾布力,杨洁,苏力坦·阿巴百克力. SDS-PAGE电泳在鉴别用牛乳掺伪的新疆特种乳中的应用[J].安徽农业科学,2016,44(23):99-102.
[6]侯艳霞,古丽巴哈尔·卡吾力,高晓黎.SDS-PAGE电泳法对4种鲜奶蛋白质组分的分析研究[J].新疆医科大学学报,2017,40(5):646-650.
[7]张婷,史晋绒,赵田田,等.四种鱼的血清、体表黏液SDSPAGE分析[J].四川动物,2015,34(6):880-884.
[8]孙克江,张甜,曲梅丽,等.聚丙烯酰胺凝胶电泳法快速检测植物源性食品中的蛋白成分[J].食品安全质量检测学报,2017,8(3):895-899.
[9]杨萧帆.蛋白电泳在小麦种子真实性及纯度鉴定中的应用[J].乡村科技,2017(21):63-66.
[10]刘姝,王凯民.肉类食品中猪源性成分检测方法之探讨[J].工程技术:全文版,2016(5):243-244.
[11] Giovannacci I,Guizard C,Carlier M,et al. Species identification of meat products by ELISA[J].International Journal of Food Science and Technology,2010,39(8):863-867.
[12]Hsieh M K,Shih P Y,Wei C F,et al.Detection of undeclared animal by-products in commercial canine canned foods:Comparative analyses by ELISA and PCR-RFLP coupled with slab gel electrophoresis or capillary gel electrophoresis[J].Journal of the Science of Food and Agriculture,2016,96(5):1659-1665.
[13]Li C,Zhang Y,Eremin S A,et al.Detection of kanamycin and gentamicin residues in animal-derived food using Ig Y antibody based ic-ELISA and FPIA[J]. Food Chemistry,2017,227:48-54.
[14]Chen X,Li Z,Guo J,et al. Simultaneous screening for marbofloxacin and ofloxacin residues in animal-derived foods using an indirect competitive immunoassay[J]. Food and Agricultural Immunology,2017,28(3):489-499.
[15]杨典原.氟苯尼考残留酶免疫法的研究[D].太原:山西大学,2016.
[16]赵秋伶,史素青,张尤华,等.酶联适体分析法检测动物源性食品中的卡那霉素残留[J].食品工业科技,2015,36(10):86-89.
[17]Claeys Erik,Vossen Els,De Smet Stefaan.Determination ofα-tocopherol by reversed-phase HPLC in feed and animalderived foods without saponification[J]. Journal of the Science of Food and Agriculture,2016,96(2):522-529.
[18]Zhu H,Yang S,Zhang Y,et al. Simultaneous detection of fifteen biogenic amines in animal derived products by HPLC-FLD with solid-phase extraction after derivatization with dansyl chloride[J].Analytical Methods,2016,8(18):3747-3755.
[19]池永红,云雅光.SPE-HPLC检测动物源性食品中3种氟喹诺酮类药物残留[J].食品研究与开发,2015(20):152-155.
[20]杨朝琳.高效液相色谱-串联质谱法检测动物源性食品中的抗生素[D].金华:浙江师范大学,2016.
[21]郝杰,姜洁,余建龙,等.固相萃取-超高效液相色谱-串联质谱法同时测定动物源性食品中多种兽药残留[J].食品科学,2017,38(12):266-272.
[22]Fei G,Zhou S,Han L,et al.A novel FT-IR spectroscopic method based on lipid characteristics for qualitative and quantitative analysis of animal-derived feedstuff adulterated with ruminant ingredients[J].Food Chemistry,2017,237:342.
[23]Gao F,Han L,Yang Z,et al.Technical note:Characterization of lipid constitution in Fourier transform infrared spectra and spectroscopic discrimination of animal-derived feedstuffs fromdifferent species[J]. Journal of Animal Science,2017,95(6):2794.
[24]吕程序,杨增玲,韩鲁佳,等.饲料中动物源性成分显微近红外光谱检测分析[J].农业机械学报,2011,42(5):171-174.
[25]Chikuni K,Ozutsumi K,Koishikawa T,et al. Species identification of cooked meats by DNA hybridization assay[J].Meat Science,1990,27(2):119-128.
[26]朱业培,王玮,吕青骎,等.基于基因芯片技术检测6种动物源性成分[J].南京农业大学学报,2015,38(6):1003-1008.
[27]石丰运,缪建锟,张利平,等.运用基因芯片技术检测牛、山羊、猪和鸡源性成分[J].生物工程学报,2010,26(6):823-829.
[28]王振全.基因芯片方法检测六种动物源性人兽共患病病原[D].长春:吉林大学,2012.
[29]Vences,M,Thomas,M,Bonett,RM,et al. Deciphering amphibian diversity through DNA barcoding:chances and challenges[J]. Philosophical Transactions of the Royal Society of London,2005,360(1462):1859-1868.
[30]Holmes B H,Steinke D,Ward R D. Identification of shark and ray fins using DNA barcoding[J].Fisheries Research,2009,95(2):280-288.
[31]Hellberg R S,Kawalek M D,Van K T,et al.Comparison of DNA extraction and PCR setup methods for use in highthroughput DNA barcoding of fish species[J]. Food Analytical Methods,2014,7(10):1950-1959.
[32]Shuang W,Yong-Bo L I,Chao-Feng M A,et al.Cytochrome C oxidase subunit I sequence as a DNA barcode to identify common meat species[J].Modern Food Science and Technology,2016,32(1):188-193.
[33]Wang M Y,Zhao Q Z,Liu B,et al.Species authority of fish and its derived products in Shanghai by DNA barcoding[J].Science and Technology of Food Industry,2016.
[34]Song T P,Yang L X,Qiu H L,et al. Loop-mediated isothermal amplification assay for identification of animal derived materials[J].Journal of Food Safety and Quality,2017.
[35]杨丽霞,付淑君,彭新凯.环介导等温扩增法检测牛羊肉中的猪肉成分[J].食品与机械,2013(5):63-65.
[36]李向丽,刘垚,谭贵良,等.基于LAMP法快速检测羊肉及其制品中的猪、鸭和羊源性成分[J].中国食品卫生杂志,2015,27(3):247-252.
[37]唐小春,卢新,冉光耀,等.一种基于lamp方法快速检测肉类掺假的试剂盒:中国,104404145 B[P].2015.
[38]李莎,张如胜,陈静芳.肠道病毒71型RT-LAMP快速检测方法的建立及初步应用[J].国际检验医学杂志,2017,38(4):470-472.
[39]陈希文,汪谦,尹苗,等.猪繁殖与呼吸综合征病毒RTLAMP快速检测方法的建立[J].中国畜牧兽医,2017,44(6):1630-1636.
[40]刘星星,耿毅,孙娜,等.兔出血症病毒可视化RT-LAMP检测方法的建立与应用[J].中国兽医科学,2017(6):687-693.
[41]熊炜,王艳,魏晓锋,等.可视化猪肺炎支原体LAMP检测方法的建立[J].中国动物传染病学报,2017,25(4):29-33.
[42]吕二盼.动物源性食品中各种动物源性肉及肉制品鉴别检验的研究[D].保定:河北农业大学,2012.
[43]Shinoda N,Kusama T,Yoshida T,et al. Developing PCR primers using a new computer program for detection of multiple animal-derived materials in feed[J].Journal of Food Protection,2008,71(11):2257.
[44]Zeng S,Qin Z,Ruan Z,et al.Multiplex fluorescent real-time PCR detection of bovine,goat and sheep derived materials in animal products[J]. Chinese Journal of Biotechnology,2009,25(1):139-146.
[45]Ali M E,Razzak M A,Hamid S B,et al.Multiplex PCR assay for the detection of five meat species forbidden in Islamic foods[J].Food Chemistry,2015,177:214-224.
[46]Hou B,Meng X,Zhang L,et al.Development of a sensitive and specific multiplex PCR method for the simultaneous detection of chicken,duck and goose DNA in meat products[J]. Meat Science,2015,101:90-94.
[47]Tao T,Chen Q,Bie X,et al.Investigation on prevalence of Listeria,spp.and Listeria monocytogenes,in animal-derived foods by multiplex PCR assay targeting novel genes[J]. Food Control,2016,26(9):704-711.
[48]Xu R,Wei S,Zhou G,et al.Multiplex Taq Man locked nucleic acid real-time PCR for the differential identification of various meat and meat products[J].Meat Science,2017,137:41.
[49]Chen X T,Zhen hua K E.Research on multiplex RT-PCR detection method of animal and plant derived ingredients in goat milk[J]. Food Research and Development,2017,38(15):156-162.