小偃麦渗入系耐盐性鉴定及其在F_2群体中的遗传分析
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摘要
小麦耐盐品种选育对于改良和利用盐碱地、增加粮食产量具有重要意义。CH7124是由小麦和中间偃麦草Z1141杂交、回交而来的高代渗入系,在苗期对盐胁迫环境表现出高度耐受性,耐盐表现与其外源亲本Z1141和小偃麦TAI8335相似。对CH7124/绵阳11的F_1及F_2群体耐盐鉴定结果表明,CH7124的耐盐性可能受2个以上QTL控制。采用分离群体分组分析法,以100个SSR标记对亲本CH7124和绵阳11以及F2群体的耐盐/敏感池进行筛选,结果获得2个多态性标记Xwmc175和Xgwm213。根据中国春缺体-四体鉴定结果,推测CH7124的2B和5B染色体上可能各存在一个耐盐相关QTL。
Screening salt-tolerant wheat varieties can enhance utilization of saline land efficiency and increase the yield of crops for the world. A wheat-Thinopyrum intermedium introgression line CH7124, developed by backcrossing TAI8335 with wheat variety Mianyang 11, was found to be a salt-tolerance material by salt stress response test at the seedling stage. The salt-tolerance phenotype of CH7124 was similar to TAI8335 and Z1141. Then, the salt-tolerance identification results of F_1 and F_2 generations from CH7124/MY11 indicated that two or more QTLs related to salt-tolerance were in CH7124. According to bulked segregant analysis, two SSR markers,Xwmc175 and Xgwm213, might be linked to salt-toletance. And based on marker loci, the salt-tolerance-related QTLs were located on chromosome 2B and 5B.
Screening salt-tolerant wheat varieties can enhance utilization of saline land efficiency and increase the yield of crops for the world. A wheat-Thinopyrum intermedium introgression line CH7124, developed by backcrossing TAI8335 with wheat variety Mianyang 11, was found to be a salt-tolerance material by salt stress response test at the seedling stage. The salt-tolerance phenotype of CH7124 was similar to TAI8335 and Z1141. Then, the salt-tolerance identification results of F_1 and F_2 generations from CH7124/MY11 indicated that two or more QTLs related to salt-tolerance were in CH7124. According to bulked segregant analysis, two SSR markers,Xwmc175 and Xgwm213, might be linked to salt-toletance. And based on marker loci, the salt-tolerance-related QTLs were located on chromosome 2B and 5B.
引文
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[2]Hajkowicz S,Young M D.An economic analysis of revegetation for dry land salinity control on the lower Eyre Peninsula in South Australia[J].Land Degradation and Development,2002,13:417-428.
[3]胡云霖,张湘泉,杨庆铭.冬小麦新品种TBT6号的选育[J].天津农业科学,1995,1(3):15-16.
[4]裴自友,温辉芹,任永康,等.小麦的耐盐性及其改良研究进展[J].作物研究,2012,26(1):93-98.
[5]赵松山,王奉芝,陆丽,等.抗旱耐盐型小麦品种沧6001的选育[J].华北农学报,2000,15(增刊):113-117.
[6]魏景芳,秦君,王淳,等.小麦与多枝赖草耐盐纯合易位系的培育及GISH鉴定[J].华北农学报,2004,19(1):40-43.
[7]Richard J.Australian researchers develop highest-yielding salt-tolerant wheat[EB/OL].[2010-04-26].http://www.sciencedaily.com/rel eases/2010/04/100423094622.htm.
[8]王洪刚,刘树兵,亓增军,等.中间偃麦草在小麦遗传改良中的应用研究[J].山东农业大学学报:自然科学版,2000,31(3):333-336.
[9]Li H,Wang X.Thinopyrum ponticum and Th.intermedium:the promising source of resistance to fungal and viral diseases of wheat[J].J Genet Genomics,2009,36:557-565.
[10]亓晓蕾.八倍体小偃麦混合基因组形成的遗传基础研究和小偃麦种质系的鉴定[D].泰安:山东农业大学,2014.
[11]徐林涛,马莹雪,张超.抗白粉病耐盐小麦-中间偃麦草附加系山农120211的鉴定[J].西北植物学报,2014,34(9):1757-1763.
[12]Chang Z,Zhang X,Yang Z,et al.Characterization of a partial wheat-Thinopyrum intermedium amphiploid and its reaction to fungal diseases of wheat[J].Hereditas,2010,147:304-312.
[13]李欣,张晓军,张丛卓,等.兼抗白粉、条锈病小偃麦渗入系CH7124抗性遗传及细胞学鉴定[J].植物遗传资源学报,2012,13(4):577-582.
[14]Zhan H X,Zhang X J,Li G R,et al.Molecular characterization of a new wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust[J].Int J Mol Sci,2015,16:2162-2173.
[15]李建波,乔麟轶,李欣,等.小麦-中间偃麦草渗入系抗白粉病基因Pm CH7124的分子定位[J].作物学报,2015,41(1):49-56.
[16]Yoshida S,Forno D A,Cock J H,et al.Laboratory manual for physiological studies of rice[M].Philippines:The International Rice Research Institute,1972:62-64.
[17]中华人民共和国农业部.NY/PZT 001—2002小麦耐盐性鉴定评价技术规范[S].北京:中国农业出版社,2000.
[18]Somers D J,Isaac P,Edwards K.A high density microsatellite consensus map for bread wheat(Triticum aestivum L.)[J].Theor Appl Genet,2004,109:1105-1114.
[19]刘旭,史娟,张学勇,等.小麦耐盐种质的筛选鉴定和耐盐基因的标记[J].植物学报,2001,43(9):945-954.
[20]Quarrie S A,Steed A,Calestani C.A high-density genetic map of hexaploid wheat(Triticum aestivum L.)from the cross Chinese Spring×SQ1 and its use to compare QTLs for grain yield across a range of environments[J].Theor Appl Genet,2005,110(5):865-880.
[21]Quarrie S A.Genetic variability and heritability of drought-induced abscisic acid accumulation in spring wheat[J].Plant Cell Environ,1981,4:147-151.