黄体酮联合保胎无忧片对仔鼠生长发育和学习记忆能力的影响
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摘要
目的:观察黄体酮联合保胎无忧片对仔鼠生长发育和学习记忆的影响。方法:将已受孕鼠随机分为空白对照组、黄体酮0.3g/kg组、保胎无忧片5g/kg组、黄体酮联合保胎无忧片(0.3+5)g/kg组。观察实验各组药物干预后母鼠一般情况、体重、分娩及哺乳情况;观察仔鼠的体重、生理发育、新生反射,采用Morris水迷宫检测仔鼠学习记忆能力,采用自发活动视频分析系统检测仔鼠自发活动情况,透射电镜观察仔鼠海马CA1区神经元和髓鞘超微结构。结果:与空白对照组比较,黄体酮联合保胎无忧片组可显著提高仔鼠体重;其余指标未见明显差异。结论:黄体酮(0.3 g/kg)联合保胎无忧片(5 g/kg)对仔鼠生长发育和学习记忆未见明显不良影响。
Objective: To observe the effect of Baotaiwuyoupian( BWP) combined with progesterone on the growth and development and learning and memory ability in newborn rats. Methods: The pregnant rats were randomized divided into the control group,0. 3 g/kg progesterone group,5 g/kg BWP group,and the combined medication( 5 g/kg BWP and 0. 3 g/kg progesterone) group. After the administration,the general condition,body weight and delivery-lactation condition in pregnant rats were observed. Meanwhile,the body weight,physical development,newborn reflect condition of newborn rats were observed. The learning and memory ability was determined by Morris water maze,and the spontaneous activity was detected by spontaneous activity video analyzer. The ultrastructure of neuron and myelin sheath in CA1 region of the Hippocampus was observed by transmission electron microscopy. Results: Compared with the control group,the body weight of newborn rats was significantly increased in the combined medication group. There was no significant difference in other indicators among groups. Conclusion: There is no harmful impacts of Baotaiwuyoupian( 5 g/kg) combined with progesterone( 0. 3 g/kg) on the growth and development and learning and memory ability in newborn rats.
Objective: To observe the effect of Baotaiwuyoupian( BWP) combined with progesterone on the growth and development and learning and memory ability in newborn rats. Methods: The pregnant rats were randomized divided into the control group,0. 3 g/kg progesterone group,5 g/kg BWP group,and the combined medication( 5 g/kg BWP and 0. 3 g/kg progesterone) group. After the administration,the general condition,body weight and delivery-lactation condition in pregnant rats were observed. Meanwhile,the body weight,physical development,newborn reflect condition of newborn rats were observed. The learning and memory ability was determined by Morris water maze,and the spontaneous activity was detected by spontaneous activity video analyzer. The ultrastructure of neuron and myelin sheath in CA1 region of the Hippocampus was observed by transmission electron microscopy. Results: Compared with the control group,the body weight of newborn rats was significantly increased in the combined medication group. There was no significant difference in other indicators among groups. Conclusion: There is no harmful impacts of Baotaiwuyoupian( 5 g/kg) combined with progesterone( 0. 3 g/kg) on the growth and development and learning and memory ability in newborn rats.
引文
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16 李烨,侯石,靳洪涛,等.部分临床常用中药的生殖毒性研究概况 .癌变·畸变·突变,2014,26(6)∶471～475.
17 吴金泽,张树峰,孟欣,等.中药生殖毒性研究进展 .河北北方学院学报(自然科学版),2015,31(6)∶113～116.
2 杨倩倩,张鹏,戴侃纯,等.神经甾体激素对神经元的影响及在神经退行性疾病治疗中的作用 .解剖学报,2014,45(3)∶424～429.
3 胡志英,方马荣,王静.外源性孕酮对去势大鼠学习记忆的影响 .医学研究杂志,2012,41(10)∶165～168.
4 夏卉芳,李啸红.菟丝子水提液对大鼠的急性毒性及微核试验 .中国实验方剂学杂志,2014,20(13)∶185～188.
5 刘茂生,李啸红,兰美兵,等.艾叶油对小鼠的遗传毒理学研究 .中药药理与临床,2012,28(2)∶85～87.
6 朱红波,朱玉平,张天宝.黄芪甲苷对大鼠和兔发育毒性的评价 .毒理学杂志,2007,21(4)∶317～318.
7 赵奎君,梁丽娟.中药保胎药研究现状及安全性评价 .药物流行病学杂志,2010,19(12)∶699～702.
8 张晓东.近年申请注册的中药新药生殖毒性研究情况及有关问题分析 .中国新药杂志,2017,26(18)∶2181～2184.
9 裴小静,尤昭玲.中药妇科用药生殖毒性、遗传毒性研究概况 .中国实验方剂学杂志,2009,15(6)∶86～87.
10 邱丽,骆世存,李明先.常用保胎中药生殖毒性的研究概况 .河北医学,2010,16(2)∶235～237.
11 赵丽颖.中药生殖发育及胚胎毒性试验研究进展 .时珍国医国药,2015,26(3)∶697～698.
12 谢辉辉,徐建亚,单进军,等.妊娠相关中药的生殖毒性研究进展 .中华中医药杂志,2015,30(7)∶2428～2430.
13 孟欣,张树峰,吴金泽.中药生殖毒性的研究概况 .承德医学院学报,2016,33(1)∶35～37.
14 卢晓燕,尤昭玲.中药生殖胚胎毒性的回顾和展望 .中医药导报,2009,15(1)∶89～91.
15 韩佳寅,易艳,梁爱华,等.中药生殖毒性研究思路和方法 .药学学报,2014,49(11)∶1498～1503.
16 李烨,侯石,靳洪涛,等.部分临床常用中药的生殖毒性研究概况 .癌变·畸变·突变,2014,26(6)∶471～475.
17 吴金泽,张树峰,孟欣,等.中药生殖毒性研究进展 .河北北方学院学报(自然科学版),2015,31(6)∶113～116.