超声预处理对剩余污泥酶活性的影响
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摘要
为了研究不同工况下超声波预处理对剩余污泥酶活性变化的影响,对剩余污泥进行不同输入能量、频率的超声预处理,测定了预处理后污泥蛋白酶和脱氢酶的变化规律。结果表明:1)超声预处理在低能量输入条件下对污泥蛋白酶和脱氢酶活性存在促进作用,而随着超声能量的进一步增加,酶活性逐渐降低直至出现抑制现象;2)超声频率对污泥酶活性影响较为显著,其中20 k Hz超声频率对蛋白酶和脱氢酶活性的促进效果最高,其次分别为25和33 k Hz;3)当输入能量高于8000 k J·(kg TS)~(-1)后,25和33 k Hz频率超声预处理对污泥蛋白酶和脱氢酶活性存在抑制效应,引起该现象的原因分析可能是高频超声空化现象及伴随的热化学效应引起的生物酶失活导致的。
To study the influence of ultrasonic pretreatment of waste-activated sludge( WAS) floc on enzyme activity,experiments were performed at various ultrasonic-specific energies and frequencies.Protease and dehydrogenase activity in WAS was examined.The activity of these enzymes increased with increasing ultrasonic-specific energy,then decreased and stabilized.At an ultrasonic frequency of 20 k Hz,both the activity of protease and dehydrogenase increased at various ultrasonic-specific energies.Where the ultrasonic frequency increased to 25 and 33 k Hz,the activity of the abovementioned enzymes decreased when ultrasonic-specific energy was over8 000 k J·( kg TS)~(-1).This might have been caused by a thermal-chemical effect followed by cavitation during ultrasonic pretreatment,leading to enzyme inactivation.
To study the influence of ultrasonic pretreatment of waste-activated sludge( WAS) floc on enzyme activity,experiments were performed at various ultrasonic-specific energies and frequencies.Protease and dehydrogenase activity in WAS was examined.The activity of these enzymes increased with increasing ultrasonic-specific energy,then decreased and stabilized.At an ultrasonic frequency of 20 k Hz,both the activity of protease and dehydrogenase increased at various ultrasonic-specific energies.Where the ultrasonic frequency increased to 25 and 33 k Hz,the activity of the abovementioned enzymes decreased when ultrasonic-specific energy was over8 000 k J·( kg TS)~(-1).This might have been caused by a thermal-chemical effect followed by cavitation during ultrasonic pretreatment,leading to enzyme inactivation.
引文
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[16]姚炜婷,孙水裕,郑莉,等.超声波-缺氧/好氧消化过程污泥EPS、酶活性及菌落数的变化研究[J].环境科学学报,2011,31(9):1925-1932
[17]龙腾锐,蒋洪波,丁文川.不同工况的低强度超声波处理对活性污泥活性的影响[J].环境科学,2007,28(2):392-396
[18]丁文川.低强度超声波辐射活性污泥的生物效应及其应用试验研究[D].重庆:重庆大学,2007
[19]KHANAL S K,GREWELL D,SUNG S,et al.Ultrasound applications in wastewater sludge pretreatment:A review[J].Critical Reviews in Environmental Science and Technology,2007,37(4):277-313
[20]PILLI S,BHUNIA P,YAN S,et al.Ultrasonic pretreatment of sludge:A review[J].Ultrasonics Sonochemistry,2011,18(1):1-18
[21]LE N T,JULCOUR-LEBIGUE C,DELMAS H.Ultrasonic sludge pretreatment under pressure[J].Ultrasonics Sonochemistry,2013,20(5):1203-1210
[2]TIEHM A,NICKEL K,ZELLHORN M,et al.Ultrasonic waste activated sludge disintegration for improving anaerobic stabilization[J].Water Research,2001,35(8):2003-2009
[3]刘贺清,熊娅,林秀军,等.超声波对污泥水解的促进作用[J].环境工程学报,2013,7(9):3573-3577
[4]许晓路,申秀英,蒋锦青.活性污泥系统中酶研究进展[J].环境科学,1990(06):54-58
[5]叶运弟,孙水裕,郑莉,等.超声波促进好氧/缺氧污泥消化过程中水解酶活性变化研究[J].环境科学,2012,33(8):2780-2785
[6]尹军.消化污泥脱氢酶活性检测的若干问题[J].中国给水排水,2000,16(10):47-49
[7]蒋洪波.低强度低频率超声对活性污泥活性的影响研究[D].重庆:重庆大学,2006
[8]严冰,王拓,赵乐军.超声波破解对于剩余污泥酶活力的影响[J].环境工程学报,2015,9(2):925-928
[9]YU G,HE P,SHAO L,et al.Extracellular proteins,polysaccharides and enzymes impact on sludge aerobic digestion after ultrasonic pretreatment[J].Water Research,2008,42(8/9):1925-1934
[10]PILLI S,BHUNIA P,YAN S,et al.Ultrasonic pretreatment of sludge:A review[J].Ultrasonics Sonochemistry,2011,18(1):1-18
[11]GONZE E,PILLOT S,VALETTE E,et al.Ultrasonic treatment of an aerobic activated sludge in a batch reactor[J].Chemical Engineering and Processing:Process Intensification,2003,42(12):965-975
[12]FENG X,DENG J,LEI H,et al.Dewaterability of waste activated sludge with ultrasound conditioning[J].Bioresource Technology,2009,100(3):1074-1081
[13]诸一殊,余光辉,何品晶,等.超声波预处理提高污泥好氧消化性能研究[J].环境工程学报,2008,2(5):690-693
[14]尹军,周春生.TTC-脱氢酶活性检测方法的研究[J].环境科学学报,1996,16(4):400-405
[15]CHU C P,CHANG B,LIAO G S,et al.Observations on changes in ultrasonically treated waste-activated sludge[J].Water Research,2001,35(4):1038-1046
[16]姚炜婷,孙水裕,郑莉,等.超声波-缺氧/好氧消化过程污泥EPS、酶活性及菌落数的变化研究[J].环境科学学报,2011,31(9):1925-1932
[17]龙腾锐,蒋洪波,丁文川.不同工况的低强度超声波处理对活性污泥活性的影响[J].环境科学,2007,28(2):392-396
[18]丁文川.低强度超声波辐射活性污泥的生物效应及其应用试验研究[D].重庆:重庆大学,2007
[19]KHANAL S K,GREWELL D,SUNG S,et al.Ultrasound applications in wastewater sludge pretreatment:A review[J].Critical Reviews in Environmental Science and Technology,2007,37(4):277-313
[20]PILLI S,BHUNIA P,YAN S,et al.Ultrasonic pretreatment of sludge:A review[J].Ultrasonics Sonochemistry,2011,18(1):1-18
[21]LE N T,JULCOUR-LEBIGUE C,DELMAS H.Ultrasonic sludge pretreatment under pressure[J].Ultrasonics Sonochemistry,2013,20(5):1203-1210