酶联免疫吸附法与免疫层析法检测IgA肾病血尿NGAL的敏感性及特异性分析
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摘要
目的对比酶联免疫吸附法(ELISA)与免疫层析法检测原发性IgA肾病(IgAN)中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的敏感性及特异性。方法选择77例经肾穿刺活检病理确诊为原发性IgA肾病的患者及体检中心随机抽取90例健康人,分别用ELISA与免疫层析法检测血、尿NGAL(sNGAL、uNGAL),对比分析两种方法的敏感性及特异性。结果将实验所得结果进行统计学分析,差异有统计学意义(P<0.05)。ELISA法所测数据sNGAL敏感性0.973,特异性1,P<0.05;uNGAL敏感性0.836,特异性1,P<0.05。免疫层析法测得数据得出sNGAL与uNGAL敏感性与特异性差异无统计学意义;sNGAL P<0.055,uNGAL数值为<50.00 ng/mL,无法进行统计学分析。结论检测IgAN患者sNGAL、uNGAL用ELISA法比免疫层析法更具敏感性与特异性,sNGAL、uNGAL更具特异性。
Objective To compare the sensitivity and specificity of the enzyme linked immunosorbent assay(ELISA) and immunohistochemical chromatography(immunchromatography) in the detection of the Neutrophil gelatinas-associated lipocalin(NGAL) associated with the gelatinase in primary IgA nephropathy.Methods 77 patients with primary IgA nephropathy confirmed by renal biopsy and 90 healthy persons randomly selected from the physical examination center were collected. NGAL in blood and urine(sNGAL and uNGAL) were detected by ELISA and immunochromatography respectively. The sensitivity and specificity of the two methods were compared and analyzed.Results Statistical analysis of experimental results showed that there was significant difference between the two groups(P<0.05). According to the data measured by ELISA,the sensitivity and specificity of sNGAL were 0.973 and 1,P<0.05,and UNGALwere 1 and 0.836,P<0.05. The sensitivity and specificity of sNGAL and uNGAL were not statistically significant by immunochromatography: sNGAL P<0.05 and uNGAL value<50.00 ng/mL.Conclusion The ELISA method is more sensitive and specific than the immunochromatography in detecting sNGAL and uNGAL in patients with IgAN,and sNGAL and uNGAL are more specific.
Objective To compare the sensitivity and specificity of the enzyme linked immunosorbent assay(ELISA) and immunohistochemical chromatography(immunchromatography) in the detection of the Neutrophil gelatinas-associated lipocalin(NGAL) associated with the gelatinase in primary IgA nephropathy.Methods 77 patients with primary IgA nephropathy confirmed by renal biopsy and 90 healthy persons randomly selected from the physical examination center were collected. NGAL in blood and urine(sNGAL and uNGAL) were detected by ELISA and immunochromatography respectively. The sensitivity and specificity of the two methods were compared and analyzed.Results Statistical analysis of experimental results showed that there was significant difference between the two groups(P<0.05). According to the data measured by ELISA,the sensitivity and specificity of sNGAL were 0.973 and 1,P<0.05,and UNGALwere 1 and 0.836,P<0.05. The sensitivity and specificity of sNGAL and uNGAL were not statistically significant by immunochromatography: sNGAL P<0.05 and uNGAL value<50.00 ng/mL.Conclusion The ELISA method is more sensitive and specific than the immunochromatography in detecting sNGAL and uNGAL in patients with IgAN,and sNGAL and uNGAL are more specific.
引文
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[12] 周静文,周红卫,廖蕴华,等.乌司他丁联合高容量血液滤过治疗急性肾损伤临床疗效及对尿NGAL、KIM-1和L-FABP的影响[J].疑难病杂志,2018,9:905-908,913.
[13] 张媛,赵敏.急性肾损伤生物标志物的研究进展[J].医学综述,2018,24(13):2559-2564,2570.
[14] 张亚兰,巴应贵.急性肾损伤的新型生物标志物研究进展[J].医学综述,2018,24(22):4512-4517.
[15] 张艳,王慧.保莉等,血NGAL水平与原发性IgA肾病临床进展危险因素的相关性研究[J].宁夏医学杂志,2018,40(3):221-223.
[16] 陈薪薪,陈朝生,陈宇,等.血、尿NGAL与IgA肾病临床与病理的相关性研究[J].中国中西医结合肾病杂志,2013,14(3):223-226.
[2] Du B,Jia Y,Zhou W,et al.Efficacy and safety of mycophenolate mofetil in patients with IgA nephropathy:an update meta-analysis[J].BMC Nephrology,2017,18(1):245.
[3] 朱国贞,王利华,齐悦,等.尿液NGAL检测在IgA肾病中的临床价值[J].中国药物与临床,2017,17(9):1336-1337.
[4] Rhee H,Shin N,Shin MJ,et al.High serum and urine neutrophil gelatinaseassociated lipocalin levels are Independent predictors of renal progression in patients with immunoglobulin A nephropathy[J].The Korean Journal of Internal Medicine,2015,30(3):354-361.
[5] Yu XQ,Wei Jl.Kidney disease in China:recent progress and prospects[J].Chinese Medical Journal,2009,122(17):2048-2053.
[6] 董丽婷,马志刚.IgA肾病研究进展[J].甘肃科技,2018,34(18):117-120,144.
[7] 杨琼琼.IgA肾病的诊治进展[J].中国医刊,2018,53(9):958-962.
[8] 张红,李晔.IgA肾病发病机制及发病相关因素研究进展[J].中国中西医结合肾病杂志,2018,19(4):373-374.
[9] Clerico A,Galli C,Fortunato A,et al.Neutrophil gelatinase-associated lipocalin(NGAL) as biomarker of acute kidney injury:a review of the laboratory characteristics and clinical evidences[J].Clinical Chemistry and Laboratory Medicine,2012,50(9):1505-1517.
[10] 张春雷,曾学辉,李忠新.尿液胱抑素C和NGAL联合检测在IgA肾病诊断中的应用[J].国际检验医学杂志,2016,37(6):783-784.
[11] 吴杰,段姝伟,孟金铃,等.尿NGAL在IgA肾病肾小管间质损伤评估中的意义探讨[J].标记免疫分析与临床,2015,22(4):265-269.
[12] 周静文,周红卫,廖蕴华,等.乌司他丁联合高容量血液滤过治疗急性肾损伤临床疗效及对尿NGAL、KIM-1和L-FABP的影响[J].疑难病杂志,2018,9:905-908,913.
[13] 张媛,赵敏.急性肾损伤生物标志物的研究进展[J].医学综述,2018,24(13):2559-2564,2570.
[14] 张亚兰,巴应贵.急性肾损伤的新型生物标志物研究进展[J].医学综述,2018,24(22):4512-4517.
[15] 张艳,王慧.保莉等,血NGAL水平与原发性IgA肾病临床进展危险因素的相关性研究[J].宁夏医学杂志,2018,40(3):221-223.
[16] 陈薪薪,陈朝生,陈宇,等.血、尿NGAL与IgA肾病临床与病理的相关性研究[J].中国中西医结合肾病杂志,2013,14(3):223-226.