枸杞多糖对小鼠海马神经元细胞系缺糖缺氧再灌注损伤的影响
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摘要
目的:观察枸杞多糖对HT22细胞缺糖缺氧再灌注损伤的影响。方法:构建HT22细胞缺糖缺氧再灌注损伤模型,设正常组、模型组、枸杞多糖100,50和25 mg·L~(-1)组,检测细胞存活率;并通过HE染色观察形态学改变;检测细胞内SOD,GSH-PX,MDA,T-AOC;利用流式细胞术检测线粒体膜电位。结果:枸杞多糖100和50 mg·L~(-1)组能明显提高细胞存活率,显著改善缺氧缺糖再灌注损伤引起的形态改变;可显著提高细胞内SOD,GSH-PX及T-AOC,减少MDA的产生;改善线粒体膜电位下降。结论:枸杞多糖能够显著减轻缺糖缺氧再灌注损伤引起的HT22细胞过氧化损伤。
Objective: To observe the protective effects of Lycium barbarum polysaccharides( LBP) on oxygen-glucose deprivation and reperfusion in mouse hippocampal neurons. Methods: Mouse hippocampal neurons were cultured to establish oxygen-glucose deprivation and reperfusion model. Cells were assigned to control group,model group and LBP group,and the survival rate of cells was measured; morphological changes were observed by HE staining; levels of SOD,GSH-PX,MDA,and T-AOC in the cells were evaluated; the mitochondrial membrane potential were measured. Results: LBP( 100,50 mg·L~(-1)) can significantly reduce the survival rate of nerve cells,improve cell morphology,increase cell SOD,GSH-PX,T-AOC,inhibite MDA,and improve the membrane potential of mitochondria. Conclusion: LBP have significant protective effects on oxygen-glucose deprivation and reperfusion,which may be mediated by improving the antioxidant capacity of neuronal cells.
Objective: To observe the protective effects of Lycium barbarum polysaccharides( LBP) on oxygen-glucose deprivation and reperfusion in mouse hippocampal neurons. Methods: Mouse hippocampal neurons were cultured to establish oxygen-glucose deprivation and reperfusion model. Cells were assigned to control group,model group and LBP group,and the survival rate of cells was measured; morphological changes were observed by HE staining; levels of SOD,GSH-PX,MDA,and T-AOC in the cells were evaluated; the mitochondrial membrane potential were measured. Results: LBP( 100,50 mg·L~(-1)) can significantly reduce the survival rate of nerve cells,improve cell morphology,increase cell SOD,GSH-PX,T-AOC,inhibite MDA,and improve the membrane potential of mitochondria. Conclusion: LBP have significant protective effects on oxygen-glucose deprivation and reperfusion,which may be mediated by improving the antioxidant capacity of neuronal cells.
引文
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[2]张赟,安佃云,沈腾,等.四乙酰芍药苷聚合物胶束的制备及其对大鼠脑缺血再灌注损伤的保护作用[J].中国医药工业杂志,2014,45(11):1036-1041.
[3]XIAO J,XING F,HUO J,et al.Lycium barbarum polysaccharides therapeutically improve hepatic functions in non-alcoholic steatohepatitis rats and cellular steatosis model[J].Sci Rep,2014,7(4):5587.
[4]CHEN W,CHENG X,CHEN J,et al.Lycium barbarum polysaccharides prevent memory and neurogenesis mpairments in scopolamine-treated rats[J].Plo S one,2014(9):e88076.
[5]GAO J,CHEN C,LIU Y,et al.Lycium barbarum polysaccharide improves traumatic cognition via reversingimbalance of apoptosis/regeneration in hippocampal neurons after stress[J].Life Sci,2015,15:121-124.
[6]PIIRONEN K,TIAINEN M,MUSTANOJA S,et al.Mild hypothermia after intravenous thrombolysis in patients with acute stroke:a randomized controlled trial[J].Stroke,2014,45(2):486-491.
[7]BURNS B,SHEPARD RH.DLO2in excied lungs perfused with blood containing sodium dithionite(Na2S2O4)[J].J Appl Phsiol,1979,46(1):100-110.
[8]龙建庭,游咏.氧化应激与脑卒中后痴呆转归的关系分析[J].大家健康,2016,10(1):8-9.
[9]许宗英,潘永惠.氧化应激与脑卒中后抑郁的关系[J].医学综述,2016,21(22):4182-4185.
[10]郝银菊,王洪波,王洁.枸杞多糖对小鼠脑缺血再灌注损伤后氧化应激的影响[J].宁夏医学杂志,2012,11(34):1069-1071.