红芪与炙红芪补中益气作用对比及成分差异分析
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摘要
目的比较红芪与炙红芪补中益气作用及成分差异,初步揭示红芪蜜炙炮制机制。方法建立大鼠脾气虚模型,通过红芪与炙红芪不同剂量水提液干预,以大鼠血清木糖(D-xylose)、胃泌素(GAS)、白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)为指标,对红芪与炙红芪补中益气药效作用进行比较。基于HPLC法分析红芪和炙红芪醇提液中的差异成分。结果与对照组相比,模型组大鼠血清D-xylose、GAS水平均显著降低(P<0.01),与模型组相比,除红芪低剂量组外,各给药组大鼠血清D-xylose、GAS水平均显著升高(P<0.01),且炙红芪中剂量组D-xylose、GAS水平均显著高于红芪中剂量组(P<0.05、0.01)。与对照组相比,模型组大鼠血清IL-2和TNF-α水平均显著升高(P<0.01),与模型组相比,各给药组大鼠血清IL-2和TNF-α水平均显著降低(P<0.05、0.01);与红芪中剂量组相比,炙红芪中剂量组大鼠血清IL-2和TNF-α水平均显著降低(P<0.05、0.01)。HPLC检测结果表明红芪炮制前后成分发生变化。结论炙红芪和红芪均可以明显干预和改善大鼠脾气虚证,且炙红芪的药效作用优于红芪,炙红芪与红芪中存在差异成分。
Objective To compare the effects of Hedysari Radix and Hedysari Radix Praeparata Cum Melle in Buzhong Yiqi and its chemical composition, and preliminarily reveal the mechanism of Hedysari Radix processed with honey. Methods A rat model of spleen qideficiency was established. The rats were treated with different doses of water extracts of Hedysari Radix and Hedysari Radix Praeparata Cum Melle and the content of serum D-xylose, GAS, IL-2, TNF-α were used as indicators to study the differences in the efficacy of Hedysari Radix and Hedysari Radix Praeparata Cum Melle in Buzhong Yiqi. Based on HPLC techniques, the different components in methanol extract of Hedysari Radix and Hedysari Radix Praeparata Cum Melle were analyzed. Results Compared with the blank group, the serum xylose, gastrin content in the model group were significantly decreased. Compared with the model group, except for the low dose group of Hedysari Radix, the serum xylose, and gastrin content of the rats in each administration group were significantly increased(P < 0.01), and the results in the middle dose group of Hedysari Radix were significantly higher than those in the middle dose group of Hedysari Radix Praeparata Cum Melle(P < 0.05, P < 0.01). Compared with the blank group, the serum IL-2 and TNF-α levels in the model group were significantly increased(P < 0.01). Compared with the model group, the serum IL-2 and TNF-α levels in the rats in each drug group were significantly decreased(P < 0.05, P < 0.01); Compared with the medium dose group of Hedysari Radix, the serum IL-2 and TNF-α levels in the medium dose group of honey-processing Hedysari Radix were significantly decreased(P < 0.05, P < 0.01). There were differences in the chromatographic peaks in the fingerprints of Hedysari Radix and Hedysari Radix Praeparata Cum Melle. Conclusion Both Hedysari Radix and Hedysari Radix Praeparata Cum Melle can significantly intervene and improve the syndrome of spleen qi deficiency in rats and the pharmacodynamics effect of Hedysari Radix Praeparata Cum Melle is better than that of Hedysari Radix. There are differences in the components of Hedysari Radix and Hedysari Radix Praeparata Cum Melle and these differences may be the active substances that cause differences in the efficacy.
Objective To compare the effects of Hedysari Radix and Hedysari Radix Praeparata Cum Melle in Buzhong Yiqi and its chemical composition, and preliminarily reveal the mechanism of Hedysari Radix processed with honey. Methods A rat model of spleen qideficiency was established. The rats were treated with different doses of water extracts of Hedysari Radix and Hedysari Radix Praeparata Cum Melle and the content of serum D-xylose, GAS, IL-2, TNF-α were used as indicators to study the differences in the efficacy of Hedysari Radix and Hedysari Radix Praeparata Cum Melle in Buzhong Yiqi. Based on HPLC techniques, the different components in methanol extract of Hedysari Radix and Hedysari Radix Praeparata Cum Melle were analyzed. Results Compared with the blank group, the serum xylose, gastrin content in the model group were significantly decreased. Compared with the model group, except for the low dose group of Hedysari Radix, the serum xylose, and gastrin content of the rats in each administration group were significantly increased(P < 0.01), and the results in the middle dose group of Hedysari Radix were significantly higher than those in the middle dose group of Hedysari Radix Praeparata Cum Melle(P < 0.05, P < 0.01). Compared with the blank group, the serum IL-2 and TNF-α levels in the model group were significantly increased(P < 0.01). Compared with the model group, the serum IL-2 and TNF-α levels in the rats in each drug group were significantly decreased(P < 0.05, P < 0.01); Compared with the medium dose group of Hedysari Radix, the serum IL-2 and TNF-α levels in the medium dose group of honey-processing Hedysari Radix were significantly decreased(P < 0.05, P < 0.01). There were differences in the chromatographic peaks in the fingerprints of Hedysari Radix and Hedysari Radix Praeparata Cum Melle. Conclusion Both Hedysari Radix and Hedysari Radix Praeparata Cum Melle can significantly intervene and improve the syndrome of spleen qi deficiency in rats and the pharmacodynamics effect of Hedysari Radix Praeparata Cum Melle is better than that of Hedysari Radix. There are differences in the components of Hedysari Radix and Hedysari Radix Praeparata Cum Melle and these differences may be the active substances that cause differences in the efficacy.
引文
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[18]施旭光,王闽予,翟理祥,等.补中益气汤配伍对脾气虚大鼠胃泌素及其基因表达的影响[J].中药新药与临床药理,2012,23(6):609-612.
[19]王欢,黄永坤,刘梅.胃肠激素与胃肠道功能及疾病的关系[J].医学综述,2013,19(15):2735-2738.
[20]王燕.甘肃地产药材红芪的质量评价研究[D].兰州:甘肃中医药大学,2015.
[21]许京,叶迎,王瑞海,等.甘肃一年生、二年生红芪和黄芪HPLC-ELSD指纹图谱比较[J].中国实验方剂学杂志,2018,24(6):71-76.
[2]中国药典[S].一部.2015.
[3]赵昱波,陈俊,许浚,等.红芪的化学成分及抗肿瘤作用研究进展[J].中草药,2015,46(22):3434-3440.
[4]崔方,韩增护,刘小花,等.红芪提高免疫功能活性部位的谱效关系研究[J].中草药,2016,47(1):101-105.
[5]陈心悦,李继平,柳小亚,等.红芪多糖对CCl4致小鼠肝纤维化及骨丢失的防治作用[J].中草药,2016,47(2):290-296.
[6]龚千锋.中药炮制学[M].北京:中国中医药出版社,2012.
[7]肖满珊.蜜炙黄芪化学成分分析及作用于脾气虚大鼠的药效学评价[D].广州:广东药学院,2015.
[8]吕晓明,宋囡,王德山,等.脾气虚证与肾气虚证大鼠小肠组织cAMP-PKA信号转导通路变化的比较研究[J].北京中医药大学学报,2018,41(7):579-584.
[9]赵思达,王彩霞,于漫,等.脾气虚证大鼠病理表现内在机制的实验探索[J].中华中医药学刊,2018,36(11):2635-2638.
[10]牛江涛,曹瑞,司昕蕾,等.不同蜜炙方法对红芪中毛蕊异黄酮和芒柄花素含量的影响[J].中国中医药信息杂志,2017,24(9):59-62.
[11]李成义,王燕,强正泽,等.红芪与黄芪中两个异黄酮类成分含量的比较研究[J].中国现代中药,2014,16(7):534-537.
[12]Zheng X F,Tian J S,Liu P,et al.Analysis of the restorative effect of Bu-zhong-yi-qi-tang in the spleen-qi deficiency rat model using 1H-NMR-based metabonomics[J].J Ethnopharmacol,2014,151(2):912-920.
[13]刘芬,刘艳菊,田春漫.苍术麸炒前后对脾虚证模型大鼠免疫系统及胃肠激素影响的比较研究[J].时珍国医国药,2015,26(6):1371-1373.
[14]郭元彪,应海峰,郑岚,等.健脾克癌宁联合四君子汤加减治疗脾气虚型胃癌术后患者疗效及对机体免疫功能的影响[J].现代中西医结合杂志,2017,26(12):1280-1282.
[15]蔡莹.超微生晒参对脾虚小鼠小肠形态及其运动的影响[D].长沙:湖南中医药大学,2007.
[16]祝婧,钟凌云,龚千锋,等.升麻不同炮制品对脾气虚动物胃肠功能的影响[J].中国实验方剂学杂志,2015,21(21):1-4.
[17]黄玉贤.血清法木糖吸收试验在脾虚证辨证中的价值及其可行性分析[D].广州:广州中医药大学,2012.
[18]施旭光,王闽予,翟理祥,等.补中益气汤配伍对脾气虚大鼠胃泌素及其基因表达的影响[J].中药新药与临床药理,2012,23(6):609-612.
[19]王欢,黄永坤,刘梅.胃肠激素与胃肠道功能及疾病的关系[J].医学综述,2013,19(15):2735-2738.
[20]王燕.甘肃地产药材红芪的质量评价研究[D].兰州:甘肃中医药大学,2015.
[21]许京,叶迎,王瑞海,等.甘肃一年生、二年生红芪和黄芪HPLC-ELSD指纹图谱比较[J].中国实验方剂学杂志,2018,24(6):71-76.