南昌市1例输入性登革热病例的型别鉴定及基因特征
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摘要
目的通过实验室检测技术分析1名输入性发热病例的病毒性病原体,并判定其型别,为临床防治提供实验室依据。方法将采集到的血标本采用实时荧光定量聚合酶链式反应(Real time PCR)检测登革病毒核酸,使用通用引物和型特异性引物行逆转录-套式PCR扩增保守区域特异性核酸片段进行型别鉴定,通过登革病毒E基因扩增和序列测定对登革病毒进行亚型基因分型。结果病例血标本核酸经RT-PCR和逆转录-套式PCR方法鉴定为登革病毒2型(DENV-2),基因分类属于DENV-2全球型。结论该例输入性疑似登革热病例系DENV-2全球型引起。
[Objective]To analyze the viral pathogens of an imported fever via laboratory detection,to identity its genotype,and provide evidence for clinical prevention and cure.[Methods] The collected blood samples were detected Dengue virus nucleic acid by Real time PCR.The universal primers and genotype-specific primers were adopted to conduct etiological identification via amplification of specific fragments of nucleic acid in conserved region by Nest RT-PCR.The gene subtype of Dengue virus was identified through Dengue virus E gene amplification and sequence determination.[Results] The nucleic acid of serum sample was identified as DENV-2 thorough RT-PCR and Nest RT-PCR,genetic classification showed it was the DENV-2 cosmopolitan genotype.[Conclusion] This imported case suspected dengue fever may be caused by the DENV-2 cosmopolitan genotype.
[Objective]To analyze the viral pathogens of an imported fever via laboratory detection,to identity its genotype,and provide evidence for clinical prevention and cure.[Methods] The collected blood samples were detected Dengue virus nucleic acid by Real time PCR.The universal primers and genotype-specific primers were adopted to conduct etiological identification via amplification of specific fragments of nucleic acid in conserved region by Nest RT-PCR.The gene subtype of Dengue virus was identified through Dengue virus E gene amplification and sequence determination.[Results] The nucleic acid of serum sample was identified as DENV-2 thorough RT-PCR and Nest RT-PCR,genetic classification showed it was the DENV-2 cosmopolitan genotype.[Conclusion] This imported case suspected dengue fever may be caused by the DENV-2 cosmopolitan genotype.
引文
[1]MUSTAFA MS,RASOTGI V,JAIN S,et al.Discovery of fifth serotype of dengue virus(DENV-5):A new public health dilemma in dengue control[J].Med J Armed Forces India,2015,71(1):67-70.
[2]MA XZ,ZHEN W,YANG PF,et al.First confirmation of imported dengue virus serotype 2 complete genome in urine from a Chinese traveler returning from India[J].Virol J,2014,11(1):1-5.
[3]LIU C,LIU Q,LIN H,et al.Spatial analysis of dengue fever in Guangdong Province,China,2001-2006[J].Asia Pac J Public Health,2014,26(1):58-66.
[4]SUN J,WU D,ZHOU HJ,et al.The epidemiological characteristics and genetic diversity of dengue virus during the third largest historical outbreak of dengue in Guangdong,China,in 2014[J].J Infect,2016,72(1):80-90.
[5]陈宏彬,王金章,张拥军,等.福建省2012—2014年登革病毒E蛋白基因序列研究[J].中国预防医学杂志,2016,17(6):432-436.
[6]冯云,范建华,朱进,等.云南省景洪市2013年登革热暴发的分子流行病学研究[J].中华流行病学杂志,2014,35(12):1409-1411.
[7]岳玉娟,任东升,刘起勇.2005—2013年中国大陆登革热疫情时空分布[J].疾病监测,2015,30(7):555-560.
[8]WANG W,YU B,LIN XD,et al.Reemergence and autochthonous transmission of dengue virus,Eastern China,2014[J].Emerg Infect Dis,2015,21(9):1670-1673.
[9]LAI S,HUANG Z,ZHOU H,et al.The changing epidemiology of dengue in China,1990-2014:a descriptive analysis of 25 years of nationwide surveillance data[J].BMC Med,2015,13(1):100.
[10]刘跃,胡水斌安福县2008—2011年登革热监测结果分析[J].中国公共卫生管理,2012,28(6):777.
[11]陶卉英,柳小青,李辉,等.南昌市2004—2012年虫媒传染病流行病学特征分析[J].现代预防医学,2014,41(17):3085-3087.
[12]LUSTIG Y,WOLF D,HALUTZ O,et al.An outbreak of dengue virus(DENV)type 2 Cosmopolitan genotype in Israelitravellers returning from the Seychelles,April 2017[J].Euro Surveill,2017,22(26):30563.
[13]WAMAN VP,KOLEKAR P,RAMTIRTHKAR MR,et al.Analysis of genotype diversity and evolution of Dengue virus serotype 2 using complete genomes[J].Peer J,2016,4(6):e2326.
[14]唐松,罗利群,程慧健,等.2006—2007年江西省登革热监测结果分析[J].江西医药,2008,43(12):1419-1420.
[15]SUN J,LU L,WU H,et al.Epidemiological trends of dengue in mainland China,2005-2015[J].Int J Infect Dis,2017,57(C):86-91.
[2]MA XZ,ZHEN W,YANG PF,et al.First confirmation of imported dengue virus serotype 2 complete genome in urine from a Chinese traveler returning from India[J].Virol J,2014,11(1):1-5.
[3]LIU C,LIU Q,LIN H,et al.Spatial analysis of dengue fever in Guangdong Province,China,2001-2006[J].Asia Pac J Public Health,2014,26(1):58-66.
[4]SUN J,WU D,ZHOU HJ,et al.The epidemiological characteristics and genetic diversity of dengue virus during the third largest historical outbreak of dengue in Guangdong,China,in 2014[J].J Infect,2016,72(1):80-90.
[5]陈宏彬,王金章,张拥军,等.福建省2012—2014年登革病毒E蛋白基因序列研究[J].中国预防医学杂志,2016,17(6):432-436.
[6]冯云,范建华,朱进,等.云南省景洪市2013年登革热暴发的分子流行病学研究[J].中华流行病学杂志,2014,35(12):1409-1411.
[7]岳玉娟,任东升,刘起勇.2005—2013年中国大陆登革热疫情时空分布[J].疾病监测,2015,30(7):555-560.
[8]WANG W,YU B,LIN XD,et al.Reemergence and autochthonous transmission of dengue virus,Eastern China,2014[J].Emerg Infect Dis,2015,21(9):1670-1673.
[9]LAI S,HUANG Z,ZHOU H,et al.The changing epidemiology of dengue in China,1990-2014:a descriptive analysis of 25 years of nationwide surveillance data[J].BMC Med,2015,13(1):100.
[10]刘跃,胡水斌安福县2008—2011年登革热监测结果分析[J].中国公共卫生管理,2012,28(6):777.
[11]陶卉英,柳小青,李辉,等.南昌市2004—2012年虫媒传染病流行病学特征分析[J].现代预防医学,2014,41(17):3085-3087.
[12]LUSTIG Y,WOLF D,HALUTZ O,et al.An outbreak of dengue virus(DENV)type 2 Cosmopolitan genotype in Israelitravellers returning from the Seychelles,April 2017[J].Euro Surveill,2017,22(26):30563.
[13]WAMAN VP,KOLEKAR P,RAMTIRTHKAR MR,et al.Analysis of genotype diversity and evolution of Dengue virus serotype 2 using complete genomes[J].Peer J,2016,4(6):e2326.
[14]唐松,罗利群,程慧健,等.2006—2007年江西省登革热监测结果分析[J].江西医药,2008,43(12):1419-1420.
[15]SUN J,LU L,WU H,et al.Epidemiological trends of dengue in mainland China,2005-2015[J].Int J Infect Dis,2017,57(C):86-91.