凯氏定氮法测定固体饮料中蛋白质含量的不确定度评定
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摘要
[目的]对凯氏定氮法测定固体饮料中蛋白质含量的不确定度进行评定。[方法]根据JJF 1059.1—2012标准中要求,建立了凯氏定氮法测定固体饮料中蛋白质含量的测量不确定度的数学模型,分析了凯氏定氮法中不确定度的主要来源,对已识别来源的不确定度分量进行分析评价。[结果]固体饮料中蛋白质含量为11.60%,扩展不确定度为0.46%(95%,k=2)。测量结果的不确定度主要来源于测定样品实际消耗硫酸标准溶液的体积与测量重复性,标准溶液的影响次之,样品质量、取样体积与定容的体积带来的影响可忽略不计。[结论]系统介绍了蛋白质测量不确定度的评定过程,提高了检测数据的可靠性和一致性。
[Objective] The uncertainty in determination of protein content by Kjeldahl method in solid beverage was evaluated. [Method]According to the requirements of JJF 1059. 1—2012 standard,a mathematical model for uncertainty in determination of protein content by Kjeldahl method in solid beverage was established,various sources of uncertainty were analyzed,the uncertainty components of identified sources are analyzed and evaluated. [Result] The concentration of protein was 11. 60% and the expanded uncertainty was 0. 46 %(95%,k= 2). The uncertainties of measurement result were mainly from volume determination of the actual consumption of sulfuric acid solution and measurement repeatability,the influence of standard solution on the second,the sample quality,the influence brought by sample volume and volume of constant volume was negligible. [Conclusion]The evaluation process of protein measurement uncertainty is introduced systematically,and the reliability and consistency of the data are improved.
[Objective] The uncertainty in determination of protein content by Kjeldahl method in solid beverage was evaluated. [Method]According to the requirements of JJF 1059. 1—2012 standard,a mathematical model for uncertainty in determination of protein content by Kjeldahl method in solid beverage was established,various sources of uncertainty were analyzed,the uncertainty components of identified sources are analyzed and evaluated. [Result] The concentration of protein was 11. 60% and the expanded uncertainty was 0. 46 %(95%,k= 2). The uncertainties of measurement result were mainly from volume determination of the actual consumption of sulfuric acid solution and measurement repeatability,the influence of standard solution on the second,the sample quality,the influence brought by sample volume and volume of constant volume was negligible. [Conclusion]The evaluation process of protein measurement uncertainty is introduced systematically,and the reliability and consistency of the data are improved.
引文
[1]国家质量技术监督局.测量不确定度评定与表示:JJF 1059.1—2012[S].北京:中国计量出版社,2013.
[2]中华人民共和国卫生部.食品安全国家标准食品中蛋白质的测定:GB5009.5—2010[S/OL].(2010-06-01)[2016-10-12].http://www.doc88.com/p-612721064825.html.
[3]陈永元,乐琼.乳制品中非蛋白氮含量测定的不确定度评定[J].食品安全质量检测学报,2016,7(2):829-833.
[4]解联合,汪琦,刘敏红,等.食品中亚硝酸盐含量的测量不确定度评定[J].中国食品卫生杂志,2004,16(6):511-513.
[5]林荆,林永辉,陈竞秀.凯氏定氮法测定大豆粉中蛋白质的不确定度评定[J].福建分析测试,2015,24(6):27-29.
[6]郝晓莉,赵瑛博,陈芳芳.分光光度法测定白菜中亚硝酸盐的不确定度评定[J].食品科学,2013,34(6):208-210.
[7]王红梅,雷萍,王少鸥,等.凯氏定氮法测定灵芝破壁孢子粉中粗蛋白质含量的不确定度评定[J].食用菌学报,2012(2):73-76.
[8]姬勇,陈霞,罗力力.全自动定氮仪测定大米中蛋白质含量的测量不确定度分析[J].农产品加工(学刊),2011(1):78-79,103.
[9]霍艳敏,王艳丽,王骏,等.高效液相色谱法测定婴幼儿乳粉中烟酰胺的不确定度评定[J].食品科学,2011(16):330-333.
[10]王燕.大豆水溶性蛋白含量测定的不确定度评定[J].粮食与食品工业,2011,18(5):50-53.
[11]高云宏.乳粉中蛋白质含量测定的不确定度分析[J].中国乳业,2011(11):44-45.
[12]陈雄,戴璇.奶粉中蛋白质的不确定度评定[J].食品安全导刊,2016(9):78-79.
[13]邓晓鸿,强敏,朱新生,等.食品中致病微生物检测结果的不确定度评定[J].食品研究与开发,2016,37(11):160-165.
[14]杨桂菊.气相色谱法测定食品中苯甲酸的不确定度评定[J].中国酿造,2004(2):
[2]中华人民共和国卫生部.食品安全国家标准食品中蛋白质的测定:GB5009.5—2010[S/OL].(2010-06-01)[2016-10-12].http://www.doc88.com/p-612721064825.html.
[3]陈永元,乐琼.乳制品中非蛋白氮含量测定的不确定度评定[J].食品安全质量检测学报,2016,7(2):829-833.
[4]解联合,汪琦,刘敏红,等.食品中亚硝酸盐含量的测量不确定度评定[J].中国食品卫生杂志,2004,16(6):511-513.
[5]林荆,林永辉,陈竞秀.凯氏定氮法测定大豆粉中蛋白质的不确定度评定[J].福建分析测试,2015,24(6):27-29.
[6]郝晓莉,赵瑛博,陈芳芳.分光光度法测定白菜中亚硝酸盐的不确定度评定[J].食品科学,2013,34(6):208-210.
[7]王红梅,雷萍,王少鸥,等.凯氏定氮法测定灵芝破壁孢子粉中粗蛋白质含量的不确定度评定[J].食用菌学报,2012(2):73-76.
[8]姬勇,陈霞,罗力力.全自动定氮仪测定大米中蛋白质含量的测量不确定度分析[J].农产品加工(学刊),2011(1):78-79,103.
[9]霍艳敏,王艳丽,王骏,等.高效液相色谱法测定婴幼儿乳粉中烟酰胺的不确定度评定[J].食品科学,2011(16):330-333.
[10]王燕.大豆水溶性蛋白含量测定的不确定度评定[J].粮食与食品工业,2011,18(5):50-53.
[11]高云宏.乳粉中蛋白质含量测定的不确定度分析[J].中国乳业,2011(11):44-45.
[12]陈雄,戴璇.奶粉中蛋白质的不确定度评定[J].食品安全导刊,2016(9):78-79.
[13]邓晓鸿,强敏,朱新生,等.食品中致病微生物检测结果的不确定度评定[J].食品研究与开发,2016,37(11):160-165.
[14]杨桂菊.气相色谱法测定食品中苯甲酸的不确定度评定[J].中国酿造,2004(2):