摘要
目的对黑螵蛸多糖进行分离纯化,并初步测定其活性。方法采用水提-醇沉法制备黑螵蛸粗多糖,经DEAE Sephadex A-25离子交换柱色谱和Sephadex G-75凝胶柱色谱纯化得到均一的黑螵蛸多糖组分HPP-1。采用凝胶渗透色谱,紫外光谱等技术对HPP-1进行分析,另外探究多糖的抗氧化活性、抗菌活性和胆碱酯酶抑制活性。结果黑螵蛸多糖HPP-1的相对分子质量(Mr)为13 048,不含蛋白质、核酸和色素,DPPH自由基和羟基自由基清除IC_(50)值分别为10.35±0.12,100.33±0.06μg/ml;多糖对枯草芽孢杆菌有抑制活性,最小抑菌浓度为18.8μg/ml;抑制乙酰胆碱酯酶和丁酰胆碱酯酶的IC_(50)值分别为6.52±0.03,18.26±0.26μg/ml。结论黑螵蛸多糖具有较好的抗氧化活性、抗菌活性和胆碱酯酶抑制活性,可作为潜在的抗氧化剂、抑菌剂和胆碱酯酶抑制剂进行深入研究和开发。
Objective To isolate, purifficate and preliminarily determine the activity of the polysaccharide from Mantidis O?theca(Heipiaoxiao). Methods The crude polysaccharide was obtained by hot water extration and ethanol deposition, and the polysaccharide(HPP-1) was purified using DEAE Sephadex A-25 and Sephadex G-75 column chromatography. The HPP-1 was elucidated by GPC and UV. In addition, the antioxidant activity, antibacterial activity and cholinesterase inhibitory activity of the polysaccharide were explored. Results The relative molecular weight(Mr)of the polysaccharide HPP-1 from Mantidis O?theca(Heipiaoxiao) was 13 048. It was free of protein, nucleic acid and pigments. IC_(50) values of DPPH and hydroxyl radicals scavenging in vitro were 10.35±0.13, 100.33±0.06 μg/ml, respectively. HPP-1 had inhibitory activity against Bacillus subtilis, with a minimum inhibitory concentration of 18.8 μg/ml. The IC_(50) values for inhibition of AChE and BuChE were 6.52±0.03, 18.26±0.26 μg/ml, respectively.Conclusion Mantidis O?theca(Heipiaoxiao) polysaccharide has good antioxidant activity, antibacterial activity and cholinesterase inhibitory activity, and may be found promising applications as potent antioxidant agent, bacteriostatic agent and double cholinesterase inhibitors.
引文
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