超声联合酶法提取玫瑰花总黄酮工艺条件优化
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摘要
目的:探索超声联合酶法提取玫瑰花总黄酮的最佳工艺条件。方法:以总黄酮提取率作为评价指标,分别考察酶解温度、酶质量分数、固液比、乙醇浓度、超声提取时间及超声提取温度对提取效果的影响。在单因素考察的基础上,采用正交试验设计优化提取工艺条件。结果:优化得到的工艺参数为固液比1∶25,酶解温度50℃,酶质量分数0. 4%,超声时间30 min。结论:优选的工艺合理、可行,具有提取率高、节能等优点,可为工业化生产提供参考。
Objective: The extraction process of flavonoids in Rosa rugosa by ultrasound combined with cellulose enzyme was optimized. Methods: The extraction rate of flavonoids was used as index. Based on the effects of some factors such as enzymatic temperature,enzyme mass concentration,ratio to solid,the concentration of ethanol,ultrasonic time and ultrasonic temperature,orthogonal design was applied to decide the best extraction process. Results: The optimal extraction technology was as follows: ratio to solid was 1: 25,enzymatic temperature was 50 ℃,enzyme mass concentration was 0. 4%,ultrasonic time was 30 min. Conclusion: The optimized process is reasonable and feasible,which can enhance extract-efficiency and is energy-saving. It provides reference for industrial production.
Objective: The extraction process of flavonoids in Rosa rugosa by ultrasound combined with cellulose enzyme was optimized. Methods: The extraction rate of flavonoids was used as index. Based on the effects of some factors such as enzymatic temperature,enzyme mass concentration,ratio to solid,the concentration of ethanol,ultrasonic time and ultrasonic temperature,orthogonal design was applied to decide the best extraction process. Results: The optimal extraction technology was as follows: ratio to solid was 1: 25,enzymatic temperature was 50 ℃,enzyme mass concentration was 0. 4%,ultrasonic time was 30 min. Conclusion: The optimized process is reasonable and feasible,which can enhance extract-efficiency and is energy-saving. It provides reference for industrial production.
引文
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[9]邵圣娟,李晓,卫静莉.超声波辅助纤维素酶解法提取马尾松花粉中的总黄酮[J].中成药,2016,38(1):204-207.
[10]王柏强,刘福,江承平,等.正交试验优选杜仲叶绿原酸的提取工艺[J].中国药房,2014,25(27):2527-2529.
[2]宋可珂,陈红艳,廖蓉苏,等.不同品种玫瑰花中黄酮的提取及抗氧化性[J].中国农业大学学报,2012,17(5):59-63.
[3]吕操,杜丽芬,宋红萍.玫瑰花总黄酮对乳腺增生大鼠上皮-间质转换的影响[J].中国医学院药学杂志,2016,36(16):1362-1365.
[4]黄文书,常雪花,曹利花,等.响应面法优化微波辅助提取玫瑰花总黄酮工艺[J].食品研究与开发,2011,32(5):37-40.
[5]谢琼,张益娜,李敏,等.紫外分光光度法测定玫瑰花和玫瑰花渣中的总黄酮含量[J].农产品加工学刊,2007(4):19-20.
[6]丁凤伟,刘红燕,李洪芹,等.正交试验法优化玫瑰花总黄酮的提取工艺[J].山东中医药大学学报,2011,35(3):255-257.
[7]李淑芳,桑小军.玫瑰花中总黄酮提取方法比较研究[J].中国现代药物应用,2012,6(8):12-13.
[8]王柏强,何效平,曾芝兰.酶法协同超声提取紫苏叶兑黄酮工艺条件优化[J].中华中医药学刊,2015,33(7):1741-1743.
[9]邵圣娟,李晓,卫静莉.超声波辅助纤维素酶解法提取马尾松花粉中的总黄酮[J].中成药,2016,38(1):204-207.
[10]王柏强,刘福,江承平,等.正交试验优选杜仲叶绿原酸的提取工艺[J].中国药房,2014,25(27):2527-2529.