流感病毒感染对不同年龄人群肠道菌群影响
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摘要
目的流感严重威胁人类的生命健康,至今仍然是人类常见的致死性传染病。部分流感对象会出现明显的腹泻等胃肠反应相关症状,分析流感病毒感染对肠道菌群的影响,为腹泻等并发症的防控提供依据。方法选取2017-01-01-2018-04-30秦皇岛市第一医院诊断为流行性感冒的患者为病例组。按照年龄、性别频率匹配的原则选取等量健康人为对照组。采集粪便进行优势菌鉴定、含量分析。对比病例组与对照组<14、14～60和>60岁亚组的菌属数量水平。结果病例组<14、14～60、>60岁亚组大肠埃希菌含量水平高于对照组,差异有统计学意义,P<0.05。病例组14～60岁大肠埃希菌含量高于<14和>60岁,对照组<14岁大肠埃希菌菌含量低于14～60和>60岁,差异有统计学意义,均P<0.05。<14岁大肠埃希菌含量上升水平最高,其次为14～60岁,再次为>60岁。病例组<14岁乳杆菌属含量高于14～60和>60岁,对照组<14岁乳杆菌属含量高于>60岁,差异有统计学意义,均P<0.05。<14、14～60、>60岁乳杆菌含量基本相同。病例组<14、14～60、>60岁乳杆菌属含量低于对照组,差异有统计学意义,均P<0.05。病例组<14、14～60、>60岁双歧杆菌属含量低于对照组,差异有统计学意义,均P<0.05。病例组<14岁双歧杆菌属含量高于14～60和>60岁,对照组<14岁双歧杆菌属含量高于14～60岁,14～60岁高于>60岁,差异有统计学意义,均P<0.05。结论流感病毒感染会导致大肠埃希菌、乳杆菌属、双歧杆菌属含量变化,其中<14岁的变化相对显著。
OBJECTIVE Influenza seriously threatens human life and health,which is still a common lethal infectious disease in human beings.Some patients with influenza have obvious gastrointestinal symptoms such as diarrhea.The study is to analyze the effect of influenza virus infection on intestinal flora and provide evidence for prevention and control of diarrhea and other complications.METHODS Influenza patients diagnosed in the first Hospital of Qinhuangdao City from January 1,2017 to April 30,2018 were selected as the case group.According to the principle of age and gender frequency matching,the same amount of healthy people were selected as the control group.Feces were collected for identification and analysis of dominant bacteria.The number of bacteria of less than 14 years old,14-60 years old and more than60 years old subgroup were compared in both the case group and the control group.RESULTS The level of Escherichia coli in case group less than 14,14-60,and over 60 years old was higher than that in the control group.The difference was statistically significant(P<0.05).The number of Escherichia coli of 14-60 years old patients in the case group was higher than that of other subgroup.The number of Escherichia coli of less than 14 years old people in the control group were lower than that of other subgroup,and the difference was statistically significant(P<0.05).The level of Escherichia coli increased the highest in less than 14 years old subgroup,followed by 14-60 years old and over 60 years old subgroup.The Lactobacillus content of subgroups in case group was lower than that of the control group,the difference was statistically significant(P<0.05).The content of Lactobacillus was basically the same at less than 14,14-60 and over 60 years old.The Bifidobacterium content of subgroups in the case group were lower than those in the control group.The Bifidobacterium content of less than 14 years old subgroup in the case group was higher than that in other two subgroups.It was the same in the control group.The difference was statistically significant(all P<0.05).CONCLUSIONS Influenza virus infection can cause changes in Escherichia coli,Lactobacillus and Bifidobacterium.The change is more significant for those under 14 years of age.
OBJECTIVE Influenza seriously threatens human life and health,which is still a common lethal infectious disease in human beings.Some patients with influenza have obvious gastrointestinal symptoms such as diarrhea.The study is to analyze the effect of influenza virus infection on intestinal flora and provide evidence for prevention and control of diarrhea and other complications.METHODS Influenza patients diagnosed in the first Hospital of Qinhuangdao City from January 1,2017 to April 30,2018 were selected as the case group.According to the principle of age and gender frequency matching,the same amount of healthy people were selected as the control group.Feces were collected for identification and analysis of dominant bacteria.The number of bacteria of less than 14 years old,14-60 years old and more than60 years old subgroup were compared in both the case group and the control group.RESULTS The level of Escherichia coli in case group less than 14,14-60,and over 60 years old was higher than that in the control group.The difference was statistically significant(P<0.05).The number of Escherichia coli of 14-60 years old patients in the case group was higher than that of other subgroup.The number of Escherichia coli of less than 14 years old people in the control group were lower than that of other subgroup,and the difference was statistically significant(P<0.05).The level of Escherichia coli increased the highest in less than 14 years old subgroup,followed by 14-60 years old and over 60 years old subgroup.The Lactobacillus content of subgroups in case group was lower than that of the control group,the difference was statistically significant(P<0.05).The content of Lactobacillus was basically the same at less than 14,14-60 and over 60 years old.The Bifidobacterium content of subgroups in the case group were lower than those in the control group.The Bifidobacterium content of less than 14 years old subgroup in the case group was higher than that in other two subgroups.It was the same in the control group.The difference was statistically significant(all P<0.05).CONCLUSIONS Influenza virus infection can cause changes in Escherichia coli,Lactobacillus and Bifidobacterium.The change is more significant for those under 14 years of age.
引文
[1]杨静,汪立杰,祝菲,等.中国大陆地区2016-2017监测年度流感暴发疫情流行病学特征分析[J].中国公共卫生,2018,34(6):1-4.
[2]解郑良,郭璐,刘跃建,等.重症社区获得性肺炎死亡患者的临床特征及死亡预测因素分析[J].华西医学,2015,30(9):1618-1620.
[3]吴遥.肠道菌群失调对流感病毒感染小鼠急性肺损伤的影响[J].现代预防医学,2018,45(4):694-699.
[4]李秋燕,李树然,彭镇委.探讨高铅血症儿童肠道菌群变化情况[J].中国实用医药,2017,12(7):109-110.
[5]van den Brand JM,Haagmans BL,van Riel D,et al.The pathology and pathogenesis of experimental severe acute respiratory syndrome and influenza in animal models[J].J Comp Pathol,2014,151(1):83-112.
[6]刘晓玢,周永健.微生态制剂对功能性腹泻的Meta分析[J].海南医学,2017,28(15):2555-2558.
[7]王三元,董俊芳,彭宜红.肠道菌群对肠道病毒感染的影响[J].微生物学免疫学进展,2018,46(1):85-89.
[8]王柯,万群,葛林,等.成都地区中老年人肠道菌群与其血糖、血脂代谢关系的初探[J].四川大学学报(医学版),2018,49(3):408-413.
[9]张鑫,马可,李赫,等.呼吸道病毒和呼吸道、肠道菌群的相互作用[J].中国微生态学杂志,2017,29(1):109-112.
[10]程译文,凌宗欣.肠道菌群与婴幼儿过敏性疾病[J].中国微生态学杂志,2016,28(3):352-355.
[11]陈运旺,陈石娇,林翀.反复肺部感染儿童的肠道状况研究[J].海南医学,2016,27(24):4045-4047.
[12]王和平,王玉蕾,郑跃杰,等.湿疹患儿肠道菌群高通量测序初步探索[J].中国微生态学杂志,2016,28(7):751-755.
[13]冯修武.上呼吸道感染中微生态制剂的功效研究[J].临床医药文献电子杂志,2015,2(7):1228-1229.
[14]郑志昌,陈映彤,郭娟娟,等.益生元对早产儿、肥胖及老年群体肠道菌群调节机制的研究现状[J].食品科学,2018,39(1):297-304.
[15]徐永金.微生态制剂培菲康经结肠途径给药联合补中益气丸治疗老年人肠道菌群失调相关性腹泻的临床研究[J].中国微生态学杂志,2016,28(4):420-424.
[16]金宏伟.培菲康联合藿香正气软胶囊治疗老年人肠道菌群失调性腹泻的疗效观察[J].中国药业,2013,22(18):14-15.
[2]解郑良,郭璐,刘跃建,等.重症社区获得性肺炎死亡患者的临床特征及死亡预测因素分析[J].华西医学,2015,30(9):1618-1620.
[3]吴遥.肠道菌群失调对流感病毒感染小鼠急性肺损伤的影响[J].现代预防医学,2018,45(4):694-699.
[4]李秋燕,李树然,彭镇委.探讨高铅血症儿童肠道菌群变化情况[J].中国实用医药,2017,12(7):109-110.
[5]van den Brand JM,Haagmans BL,van Riel D,et al.The pathology and pathogenesis of experimental severe acute respiratory syndrome and influenza in animal models[J].J Comp Pathol,2014,151(1):83-112.
[6]刘晓玢,周永健.微生态制剂对功能性腹泻的Meta分析[J].海南医学,2017,28(15):2555-2558.
[7]王三元,董俊芳,彭宜红.肠道菌群对肠道病毒感染的影响[J].微生物学免疫学进展,2018,46(1):85-89.
[8]王柯,万群,葛林,等.成都地区中老年人肠道菌群与其血糖、血脂代谢关系的初探[J].四川大学学报(医学版),2018,49(3):408-413.
[9]张鑫,马可,李赫,等.呼吸道病毒和呼吸道、肠道菌群的相互作用[J].中国微生态学杂志,2017,29(1):109-112.
[10]程译文,凌宗欣.肠道菌群与婴幼儿过敏性疾病[J].中国微生态学杂志,2016,28(3):352-355.
[11]陈运旺,陈石娇,林翀.反复肺部感染儿童的肠道状况研究[J].海南医学,2016,27(24):4045-4047.
[12]王和平,王玉蕾,郑跃杰,等.湿疹患儿肠道菌群高通量测序初步探索[J].中国微生态学杂志,2016,28(7):751-755.
[13]冯修武.上呼吸道感染中微生态制剂的功效研究[J].临床医药文献电子杂志,2015,2(7):1228-1229.
[14]郑志昌,陈映彤,郭娟娟,等.益生元对早产儿、肥胖及老年群体肠道菌群调节机制的研究现状[J].食品科学,2018,39(1):297-304.
[15]徐永金.微生态制剂培菲康经结肠途径给药联合补中益气丸治疗老年人肠道菌群失调相关性腹泻的临床研究[J].中国微生态学杂志,2016,28(4):420-424.
[16]金宏伟.培菲康联合藿香正气软胶囊治疗老年人肠道菌群失调性腹泻的疗效观察[J].中国药业,2013,22(18):14-15.