免疫分析法在克伦特罗残留检测中的应用
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摘要
克伦特罗能提高脂肪型动物的瘦肉和脂肪的比率,因此常被用做畜禽饲料添加剂。由于其化学性质稳定,残留在畜禽产品中的克伦特罗对人类健康产生了严重的危害,故检测畜禽产品中的克伦特罗残留对于保证食品安全非常重要。免疫分析法特异性强、灵敏度高、反应条件温和,广泛应用于食品安全检测。综述了免疫亲和色谱法、放射免疫分析法、酶联免疫分析法、化学发光免疫分析法、免疫层析法、时间分辨荧光免疫分析法、免疫传感器、分子印迹免疫分析法等免疫分析法在克伦特罗残留分析中的应用,并展望了这些分析方法的发展趋势。
Clenbuterol can increase the ratio of lean meat and fat of fatty animals,so it is often used as feed additive for livestock.Because of its stable chemical properties,the residues in livestock products have caused serious harm to human health.Detection of Clenbuterol residues in livestock products plays important role in ensuring food safety.The immunoassay,with high specificity,high sensitivity and mild reaction conditions,has been widely used in food security detection.The application of immunoassay such as immunoaffinity chromatography,radioimmunoassay,enzyme-linked immunosorbent assay,chemiluminescent immunoassay,immunochromatographic assay,time resolved fluoroimmunoassay,immunosensor and molecularly imprinted immunoassay in Clenbuterol residue detection was reviewed,and development trend of these analysis methods was also prospected.
Clenbuterol can increase the ratio of lean meat and fat of fatty animals,so it is often used as feed additive for livestock.Because of its stable chemical properties,the residues in livestock products have caused serious harm to human health.Detection of Clenbuterol residues in livestock products plays important role in ensuring food safety.The immunoassay,with high specificity,high sensitivity and mild reaction conditions,has been widely used in food security detection.The application of immunoassay such as immunoaffinity chromatography,radioimmunoassay,enzyme-linked immunosorbent assay,chemiluminescent immunoassay,immunochromatographic assay,time resolved fluoroimmunoassay,immunosensor and molecularly imprinted immunoassay in Clenbuterol residue detection was reviewed,and development trend of these analysis methods was also prospected.
引文
[1]YANG X,WU F,CHEN D Z,et al.An electrochemical immunosensor for rapid determination of clenbuterol by using magnetic nanocomposites to modify screen printed carbon electrode based on competitive immunoassay mode[J].Sensor.Actuat.B-chem.,2014,192(3):529-535.
[2]XIE Y,CHANG H F,ZHAO K,et al.A novel immunochromatographic assay(ICA)based on surface-enhanced raman scattering for the sensitive and quantitative determination of clenbuterol[J].Anal.Methods,2015,7(2):513-520.
[3]ZHOU J Y,XU X S,WANG Y X.Competitive immunoassay for clenbuterol using capillary electrophoresis with laser-induced fluorescence detection[J].J.Chromatogr.B,2007,848(2):226-231.
[4]罗贤文,魏瑞丽,李晓霞.盐酸克伦特罗的电化学行为及与鲱鱼精DNA相互作用的研究[J].化学试剂,2013,35(3):213-217.
[5]SALLERAS L,DOMINGUEZ A,MATA E,et al.Epidemiologic study of an outbreak of clenbuterol poisoning in Catalonia,Spain[J].Public Health Rep.,1995,110(3):338-342.
[6]ELLIOT C T,MCCAUGHEY W J,CROOKS S R,et al.Residues of clenbuterol in cattle receiving therapeutic doses:implications for differentiating between legal and illegal use[J].Vet.Quart.,1995,17(3):100-102.
[7]汤轶伟,王硕,励建荣,等.兴奋剂盐酸克伦特罗残留检测方法最新研究进展[J].中国食品学报,2013,13(5):158-164.
[8]中华人民共和国农业部.第235号公告,动物性食品中兽药最高残留限量[Z].2002-12-24.
[9]GAO T,YE N S,LI J.Determination of ractopamine and clenbuterol in beef by graphene oxide hollow fiber solidphase microextraction and high-performance liquid chromatography[J].Anal.Lett.,2015,49(8):1 163-1 175.
[10]YAN K P,ZHANG H Q,HUI W L,et al.Rapid screening of toxic salbutamol,ractopamine,and clenbuterol in pork sample by high-performance liquid chromatography-UV method[J].J.Food Drug Anal.,2016,24(2):277-283.
[11]WANG H L,YAN H,SHI M R,et al.An enzyme-assisted and nitrogen-blowing salt-induced solidified floating organic droplet microextraction for determination of clenbuterol and ractopamine in swine feed via capillary electrophoresis[J].Anim.Feed Sci.Tech.,2015,209(11):257-267.
[12]YE D R,WU S S,XU J Q,et al.Rapid determination of clenbuterol in pork by direct immersion solid-phase microextraction coupled with gas chromatography-mass spectrometry[J].J.Chromatogr.Sci.,2016,54(2):112-118.
[13]CHU L L,ZHENG S L,QU B,et al.Detection ofβ-agonists in pork tissue with novel electrospun nanofibersbased solid-phase extraction followed ultra-high performance liquid chromatography/tandem mass spectrometry[J].Food Chem.,2017,227(15):315-321.
[14]LIN Y P,LEE Y L,HUNG C Y,et al.Determination of multiresidue analysis ofβ-agonists in muscle and viscera using liquid chromatograph/tandem mass spectrometry with Quick,Easy,Cheap,Effective,Rugged,and Safe methodologies[J].J.Food Drug Anal.,2017,25(2):275-284.
[15]余乐,霍光华,符金华.莱克多巴胺、沙丁胺醇与克伦特罗三合一免疫亲和柱的制备及应用[J].分析测试学报,2014,33(6):693-697.
[16]王建平,史为民,沈建忠.免疫亲和色谱-气相色谱/质谱法(IAC-GC/MS)检测猪肝中的沙丁胺醇与克伦特罗[J].畜牧兽医学报,2007,38(3):271-275.
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[19]刘硕,陶晓奇.β-兴奋剂在动物性食品中残留的免疫分析方法研究进展[J].食品与发酵工业,2017,43(8):273-279.
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[21]刘旭洋,徐斐,曹慧,等.基于固定化抗体克伦特罗直接竞争ELISA检测体系的建立[J].食品与发酵科技,2014,50(6):64-69.
[22]郑海松,李云飞,杨小娇,等.出口肉及制品中盐酸克伦特罗的ELISA检测方法的建立[J].食品科技,2011,36(11):120-123.
[23]穆莉莉,沙梦梦,叶嘉明.基于酶联免疫法检测盐酸克伦特罗的微流控系统[J].食品科技,2017,42(11):322-325.
[24]MA L Y,NILGHAZ A,CHOI J R,et al.Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA[J].Food Chem.,2018,246(4):437-441.
[25]金茂俊,王静,杨丽华,等.化学发光免疫分析方法在食品安全检测中的研究进展[J].食品安全质量检测学报,2014,5(3):840-845.
[26]王建军,熊雅婷,秦誉,等.同时检测动物性食品中6种β-受体激动剂的化学发光免疫分析方法的建立[J].肉类研究,2017,31(10):36-40.
[27]王毅谦,陆慧媛,袁芳,等.竞争化学发光酶免疫测定技术检测盐酸克伦特罗[J].食品安全质量检测学报,2016,7(11):4 518-4 524.
[28]WU Y P,XU F,JIANG H Y,et al.Determination of salbutamol,clenbuterol,and brombuterol in urine by a highly sensitive chemiluminescence enzyme immunoassay[J].Anal.Lett.,2014,47(16):2 761-2 773.
[29]TAO X Q,SHEN J Z,CAO X Y,et al.Simultaneous determination of chloramphenicol and clenbuterol in milk with hybrid chemiluminescence immunoassays[J].Anal.Methods,2014,6(4):1 021-1 027.
[30]张瑞华,李芳,康怀彬,等.动物源性产品中盐酸克伦特罗检测方法的研究进展[J].食品研究与开发,2017,38(3):208-211.
[31]YAO X,YAN P P,TANG Q H,et al.Quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification for ultrasensitive detection of clenbuterol[J].Anal.Chim.Acta,2013,798(18):82-88.
[32]YAN P P,TANG Q H,DENG A P,et al.Ultrasensitive detection of clenbuterol by quantum dots based electrochemiluminescent immunosensor using gold nanoparticles as substrate and electron transport accelerator[J].Sensor.Actuat.B-chem.,2014,191(2):508-515.
[33]李超辉.胶体金免疫层析试纸条定量检测猪尿以及猪肉中的克伦特罗残留[D].南昌:南昌大学,2014.
[34]肖治理,余建华,雷红涛,等.盐酸克伦特罗快速检测胶体金试纸的研制[J].现代食品科技,2013,29(8):2 004-2 010.
[35]张金艳,熊艳,廖且根,等.CdTe量子点免疫层析试纸条检测克伦特罗[J].食品科学,2015,36(22):161-164.
[36]李丹妮,黄华,张婧,等.3种β2-受体激动剂荧光免疫层析试纸条的制备及特性研究[J].中国畜牧兽医,2017,44(8):2 437-2 442.
[37]ZHU C J,ZHAO G Y,DOU W C.Immunochromatographic assay using brightly colored silica nanoparticles as visible label for point-of-care detection of clenbuterol[J].Sensor.Actuat.B-chem.,2018,266(8):392-399.
[38]WANG W W,SU X X,OUYANG H,et al.A novel immunochromatographic assay based on a time-resolved chemiluminescence strategy for the multiplexed detection of ractopamine and clenbuterol[J].Anal.Chim.Acta,2016,917(4):79-84.
[39]WANG P P,WANG R G,ZHANG W,et al.Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol[J].Biosens.Bioelectron.,2016,77(3):866-870.
[40]解肖鹏,张雷.时间分辨荧光免疫分析技术的研究进展[J].食品与药品,2012,14(5):203-206.
[41]LI M,ZHANG Y Y,XUE Y L,et al.Simultaneous determination ofβ2-agonists clenbuterol and salbutamol in water and swine feed samples by dual-labeled time-resolved fluoroimmunoassay[J].Food Control,2017,73(3):1 039-1 044.
[42]李文进,刘霞,李蓉卓,等.电化学传感器在农药残留检测中的研究进展[J].食品与机械,2013,29(4):241-245.
[43]WEI L H,LIU L,KANG H M,et al.Development of a disposable label-free impedance immunosensor for direct and sensitive clenbuterol determination in pork[J].Food Anal.Method,2015,9(6):1 781-1 788.
[44]WEI L H,WANG T,LIU Y S,et al.Label-free microcantilever immunosensor based on a competitive immunoassay for the determination of clenbuterol[J].Anal.Lett.,2018,51(14):2 240-2 251.
[45]KABIRAZ D C,MORITA K,SAKAMOTO K,et al.Highly sensitive detection of clenbuterol in urine sample by using surface plasmon resonance immunosensor[J].Talanta,2018,186(15):521-526.
[46]柯珍,朱华,钟世安,等.分子印迹技术及其应用研究进展[J].化学研究与应用,2018,30(6):865-874.
[47]田春霞.分子印迹固相萃取技术在食品安全检测中的应用进展[J].化学试剂,2017,39(11):1 175-1 178.
[48]TANG Y W,GAO J W,LIU X Y,et al.Ultrasensitive detection of clenbuterol by a covalent imprinted polymer as a biomimetic antibody[J].Food Chem.,2017,228(8):62-69.
[49]FENG F,ZHENG J W,QIN P,et al.A novel quartz crystal microbalance sensor array based on molecular imprinted polymers for simultaneous detection of clenbuterol and its metabolites[J].Talanta,2017,167(5):94-102.
[2]XIE Y,CHANG H F,ZHAO K,et al.A novel immunochromatographic assay(ICA)based on surface-enhanced raman scattering for the sensitive and quantitative determination of clenbuterol[J].Anal.Methods,2015,7(2):513-520.
[3]ZHOU J Y,XU X S,WANG Y X.Competitive immunoassay for clenbuterol using capillary electrophoresis with laser-induced fluorescence detection[J].J.Chromatogr.B,2007,848(2):226-231.
[4]罗贤文,魏瑞丽,李晓霞.盐酸克伦特罗的电化学行为及与鲱鱼精DNA相互作用的研究[J].化学试剂,2013,35(3):213-217.
[5]SALLERAS L,DOMINGUEZ A,MATA E,et al.Epidemiologic study of an outbreak of clenbuterol poisoning in Catalonia,Spain[J].Public Health Rep.,1995,110(3):338-342.
[6]ELLIOT C T,MCCAUGHEY W J,CROOKS S R,et al.Residues of clenbuterol in cattle receiving therapeutic doses:implications for differentiating between legal and illegal use[J].Vet.Quart.,1995,17(3):100-102.
[7]汤轶伟,王硕,励建荣,等.兴奋剂盐酸克伦特罗残留检测方法最新研究进展[J].中国食品学报,2013,13(5):158-164.
[8]中华人民共和国农业部.第235号公告,动物性食品中兽药最高残留限量[Z].2002-12-24.
[9]GAO T,YE N S,LI J.Determination of ractopamine and clenbuterol in beef by graphene oxide hollow fiber solidphase microextraction and high-performance liquid chromatography[J].Anal.Lett.,2015,49(8):1 163-1 175.
[10]YAN K P,ZHANG H Q,HUI W L,et al.Rapid screening of toxic salbutamol,ractopamine,and clenbuterol in pork sample by high-performance liquid chromatography-UV method[J].J.Food Drug Anal.,2016,24(2):277-283.
[11]WANG H L,YAN H,SHI M R,et al.An enzyme-assisted and nitrogen-blowing salt-induced solidified floating organic droplet microextraction for determination of clenbuterol and ractopamine in swine feed via capillary electrophoresis[J].Anim.Feed Sci.Tech.,2015,209(11):257-267.
[12]YE D R,WU S S,XU J Q,et al.Rapid determination of clenbuterol in pork by direct immersion solid-phase microextraction coupled with gas chromatography-mass spectrometry[J].J.Chromatogr.Sci.,2016,54(2):112-118.
[13]CHU L L,ZHENG S L,QU B,et al.Detection ofβ-agonists in pork tissue with novel electrospun nanofibersbased solid-phase extraction followed ultra-high performance liquid chromatography/tandem mass spectrometry[J].Food Chem.,2017,227(15):315-321.
[14]LIN Y P,LEE Y L,HUNG C Y,et al.Determination of multiresidue analysis ofβ-agonists in muscle and viscera using liquid chromatograph/tandem mass spectrometry with Quick,Easy,Cheap,Effective,Rugged,and Safe methodologies[J].J.Food Drug Anal.,2017,25(2):275-284.
[15]余乐,霍光华,符金华.莱克多巴胺、沙丁胺醇与克伦特罗三合一免疫亲和柱的制备及应用[J].分析测试学报,2014,33(6):693-697.
[16]王建平,史为民,沈建忠.免疫亲和色谱-气相色谱/质谱法(IAC-GC/MS)检测猪肝中的沙丁胺醇与克伦特罗[J].畜牧兽医学报,2007,38(3):271-275.
[17]李超辉,熊勇华,郭亮,等.纳米免疫磁珠富集猪肉中的盐酸克伦特罗[J].食品科学,2013,34(14):182-186.
[18]韦林洪.农药多残留免疫亲和色谱技术研究[D].扬州:扬州大学,2005.
[19]刘硕,陶晓奇.β-兴奋剂在动物性食品中残留的免疫分析方法研究进展[J].食品与发酵工业,2017,43(8):273-279.
[20]GRANJA R H,MONTES NIO A M,RABONE F,et al.Validation of radioimmunoassay screening methods for beta-agonists in bovine liver according to Commission Decision 2002/657/EC[J].Food Addit.Contam.,2008,25(12):1 475-1 481.
[21]刘旭洋,徐斐,曹慧,等.基于固定化抗体克伦特罗直接竞争ELISA检测体系的建立[J].食品与发酵科技,2014,50(6):64-69.
[22]郑海松,李云飞,杨小娇,等.出口肉及制品中盐酸克伦特罗的ELISA检测方法的建立[J].食品科技,2011,36(11):120-123.
[23]穆莉莉,沙梦梦,叶嘉明.基于酶联免疫法检测盐酸克伦特罗的微流控系统[J].食品科技,2017,42(11):322-325.
[24]MA L Y,NILGHAZ A,CHOI J R,et al.Rapid detection of clenbuterol in milk using microfluidic paper-based ELISA[J].Food Chem.,2018,246(4):437-441.
[25]金茂俊,王静,杨丽华,等.化学发光免疫分析方法在食品安全检测中的研究进展[J].食品安全质量检测学报,2014,5(3):840-845.
[26]王建军,熊雅婷,秦誉,等.同时检测动物性食品中6种β-受体激动剂的化学发光免疫分析方法的建立[J].肉类研究,2017,31(10):36-40.
[27]王毅谦,陆慧媛,袁芳,等.竞争化学发光酶免疫测定技术检测盐酸克伦特罗[J].食品安全质量检测学报,2016,7(11):4 518-4 524.
[28]WU Y P,XU F,JIANG H Y,et al.Determination of salbutamol,clenbuterol,and brombuterol in urine by a highly sensitive chemiluminescence enzyme immunoassay[J].Anal.Lett.,2014,47(16):2 761-2 773.
[29]TAO X Q,SHEN J Z,CAO X Y,et al.Simultaneous determination of chloramphenicol and clenbuterol in milk with hybrid chemiluminescence immunoassays[J].Anal.Methods,2014,6(4):1 021-1 027.
[30]张瑞华,李芳,康怀彬,等.动物源性产品中盐酸克伦特罗检测方法的研究进展[J].食品研究与开发,2017,38(3):208-211.
[31]YAO X,YAN P P,TANG Q H,et al.Quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification for ultrasensitive detection of clenbuterol[J].Anal.Chim.Acta,2013,798(18):82-88.
[32]YAN P P,TANG Q H,DENG A P,et al.Ultrasensitive detection of clenbuterol by quantum dots based electrochemiluminescent immunosensor using gold nanoparticles as substrate and electron transport accelerator[J].Sensor.Actuat.B-chem.,2014,191(2):508-515.
[33]李超辉.胶体金免疫层析试纸条定量检测猪尿以及猪肉中的克伦特罗残留[D].南昌:南昌大学,2014.
[34]肖治理,余建华,雷红涛,等.盐酸克伦特罗快速检测胶体金试纸的研制[J].现代食品科技,2013,29(8):2 004-2 010.
[35]张金艳,熊艳,廖且根,等.CdTe量子点免疫层析试纸条检测克伦特罗[J].食品科学,2015,36(22):161-164.
[36]李丹妮,黄华,张婧,等.3种β2-受体激动剂荧光免疫层析试纸条的制备及特性研究[J].中国畜牧兽医,2017,44(8):2 437-2 442.
[37]ZHU C J,ZHAO G Y,DOU W C.Immunochromatographic assay using brightly colored silica nanoparticles as visible label for point-of-care detection of clenbuterol[J].Sensor.Actuat.B-chem.,2018,266(8):392-399.
[38]WANG W W,SU X X,OUYANG H,et al.A novel immunochromatographic assay based on a time-resolved chemiluminescence strategy for the multiplexed detection of ractopamine and clenbuterol[J].Anal.Chim.Acta,2016,917(4):79-84.
[39]WANG P P,WANG R G,ZHANG W,et al.Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol[J].Biosens.Bioelectron.,2016,77(3):866-870.
[40]解肖鹏,张雷.时间分辨荧光免疫分析技术的研究进展[J].食品与药品,2012,14(5):203-206.
[41]LI M,ZHANG Y Y,XUE Y L,et al.Simultaneous determination ofβ2-agonists clenbuterol and salbutamol in water and swine feed samples by dual-labeled time-resolved fluoroimmunoassay[J].Food Control,2017,73(3):1 039-1 044.
[42]李文进,刘霞,李蓉卓,等.电化学传感器在农药残留检测中的研究进展[J].食品与机械,2013,29(4):241-245.
[43]WEI L H,LIU L,KANG H M,et al.Development of a disposable label-free impedance immunosensor for direct and sensitive clenbuterol determination in pork[J].Food Anal.Method,2015,9(6):1 781-1 788.
[44]WEI L H,WANG T,LIU Y S,et al.Label-free microcantilever immunosensor based on a competitive immunoassay for the determination of clenbuterol[J].Anal.Lett.,2018,51(14):2 240-2 251.
[45]KABIRAZ D C,MORITA K,SAKAMOTO K,et al.Highly sensitive detection of clenbuterol in urine sample by using surface plasmon resonance immunosensor[J].Talanta,2018,186(15):521-526.
[46]柯珍,朱华,钟世安,等.分子印迹技术及其应用研究进展[J].化学研究与应用,2018,30(6):865-874.
[47]田春霞.分子印迹固相萃取技术在食品安全检测中的应用进展[J].化学试剂,2017,39(11):1 175-1 178.
[48]TANG Y W,GAO J W,LIU X Y,et al.Ultrasensitive detection of clenbuterol by a covalent imprinted polymer as a biomimetic antibody[J].Food Chem.,2017,228(8):62-69.
[49]FENG F,ZHENG J W,QIN P,et al.A novel quartz crystal microbalance sensor array based on molecular imprinted polymers for simultaneous detection of clenbuterol and its metabolites[J].Talanta,2017,167(5):94-102.