水稻纹枯病菌及毒素的寄主抗性响应差异
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摘要
为探明防御酶系在水稻纹枯病菌多核菌株(Rhizoctonia solani)和双核菌株(Rhizoctonia oryzae-sativae)与水稻互作过程中的寄主抗性响应差异,选取多核菌株(LND06)和双核菌株(JLS10)进行粗毒素提取,用菌株及毒素分别接种相对抗病水稻品种港源8号和相对感病水稻品种秋田小町,测定接种后7种防御酶活性及ASAFR和H2O2含量的动态变化。结果表明:港源8号和秋田小町接种多核与双核菌株及其毒素后,体内POD、PAL、APX、PPO、CAT、T-SOD、GSH-PX活性及ASAFR和H_2O_2含量均呈先升高后降低的变化趋势。较为感病的水稻品种秋田小町与较为抗病的水稻品种港源8号相比寄主抗性响应较晚,接种多核菌株及其毒素的寄主防御酶峰值更高、达到峰值的时间更早,部分在36h达到峰值,其余在36~48h达到峰值,但达到峰值后下降速率也更快;接种双核菌株及其毒素的寄主抗性响应较晚,在48~72h达到峰值,但达到峰值后防御酶下降的速率较慢,维持在略高于对照的稳定水平。
In order to explore the difference of resistance response of host between Rhizoctonia oryzae-sativae and Rhizoctonia solani, Rhizoctonia oryzae-sativae JLS10 and Rhizoctonia solani LND06 were selected. The activities of seven defense enzymes and the contents of ASAFR and H_2O_2 were determined by inoculating two resistant and susceptible rice varieties with different strains and toxins. The results showed that two resistant and susceptible strains were inoculated with multinucleate Rhizoctonia,binucleate Rhizoctonia and their toxins, the trend of POD, PAL, APX, PPO, CAT, T-SOD, GSH-PX activities and ASAFR, H_2O_2 contents increased first and then decreased. The resistant response of the susceptible strain Akitakomachi was later than that of the resistant rice variety Gangyuan 8 after inoculation with the tested strain and its toxin. The response dynamics of host defense enzyme system after inoculation of binucleate Rhizoctonia, multinucleate Rhizoctonia and their toxins were also different. The peak value of the host defense enzyme system of the inoculated multinucleate Rhizoctonia and their toxins was higher, and the peak time was earlier at 36-48 h. But after the peak, the rate of the decrease was also faster. The host resistant response of binucleate Rhizoctonia and its toxin was relatively late(at 48-72 h), but after reaching the peak, the rate of the decrease was slow and maintained at a stable level.
In order to explore the difference of resistance response of host between Rhizoctonia oryzae-sativae and Rhizoctonia solani, Rhizoctonia oryzae-sativae JLS10 and Rhizoctonia solani LND06 were selected. The activities of seven defense enzymes and the contents of ASAFR and H_2O_2 were determined by inoculating two resistant and susceptible rice varieties with different strains and toxins. The results showed that two resistant and susceptible strains were inoculated with multinucleate Rhizoctonia,binucleate Rhizoctonia and their toxins, the trend of POD, PAL, APX, PPO, CAT, T-SOD, GSH-PX activities and ASAFR, H_2O_2 contents increased first and then decreased. The resistant response of the susceptible strain Akitakomachi was later than that of the resistant rice variety Gangyuan 8 after inoculation with the tested strain and its toxin. The response dynamics of host defense enzyme system after inoculation of binucleate Rhizoctonia, multinucleate Rhizoctonia and their toxins were also different. The peak value of the host defense enzyme system of the inoculated multinucleate Rhizoctonia and their toxins was higher, and the peak time was earlier at 36-48 h. But after the peak, the rate of the decrease was also faster. The host resistant response of binucleate Rhizoctonia and its toxin was relatively late(at 48-72 h), but after reaching the peak, the rate of the decrease was slow and maintained at a stable level.
引文
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[15]ZHANG Y,TANG HR,LUO Y.Variation in antioxidant enzyme activities of two strawberry cultivars with short term low temperature stress[J].World Journal of Agricultural Sciences,2008,4(4):458-462.
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[19]吴样孙,陈一壮,蒙信满,等.水稻纹枯病抗性反应中主要防御酶的活性变化[J].中国农学通报,2008,24(5):327-330.
[20]左示敏,陈夕军,陈红旗,等.不同抗性水平水稻品种对纹枯病菌毒素的防卫反应与生理差异[J].中国水稻科学,2014,28(5):551-558.
[21]GUO SY,CHEN JH,XIAO Y,et al.Changes of physiological characteristics and yield in peanut varieties with different resistance effected by peanut scab[J].Agricultural Science and Technology,2015,16(10):2309-2313.
[22]MISHRA BB,GAUTAM S,SHARMA A,et al.Purification and characterisation of polyphenol oxidase(PPO)from eggplant(Solanum melongena)[J].Food Chemistry,2012,134(4):1855-1861.
[23]NIKRAFTAR F,TAHEIR P,RASTEGAR MF,et al.Tomato partial resistance to Rhizoctonia solani involves antioxidative defense mechanisms[J].Physiological and Molecular Plant Pathology,2013,81(4):74-83.
[2]孟庆忠,刘志恒,王鹤影,等.水稻纹枯病害研究进展[J].沈阳农业大学学报,2001,32(5):376-381.
[3]ONIKI M,OGOSHI A,ARAKI T,et al.The perfect state of Rhizoctonia oryzae and Rhizoctonia zeae and the anastomosis groups of Waitea circinata[J].Transactions of the Mycological Society of Japan,1985,26(2):189-198.
[4]刘志恒,胡积祥,王世维,等.吉林水稻纹枯病菌多核和双核菌株生物学特性比较[J].沈阳农业大学学报,2013,44(4):428-433.
[5]魏松红,王海宁,胡积祥,等.吉林地区水稻纹枯病菌对噻呋酰胺药物的敏感基线探讨[J].沈阳农业大学学报,2016,47(3):278-282.
[6]张优,魏松红,王海宁,等.东北地区水稻纹枯病菌遗传多样性和致病性分析[J].沈阳农业大学学报,2017,48(1):9-14.
[7]陈夕军,徐艳,童蕴慧,等.水稻纹枯病菌毒素致病机理研究[J].植物病理学报,2009,39(4):439-443.
[8]YANG YQ,YANG M,LI MH,et al.Isolation and characterization of a phytotoxin from Rhizoctonia solani,the causal agent of rice sheath blight[J].Asian Journal of Chemistry,2011,23(8):3500-3508.
[9]徐艳.水稻纹枯病菌毒素的致病机理及对寄主防御酶活性的影响[D].扬州:扬州大学,2006.
[10]徐敬友,张华东,张红,等.立枯丝核菌毒素的产生及与致病力的关系[J].扬州大学学报(农业与生命科学版),2004,25(2):61-64.
[11]CHEN CQ,RICHARD R,NICOLE BENHAMOU,et al.Defense enzymes induced in cucumber roots by treatment with plant growthpromoting rhizobacteria(PGPR)and Pythium aphanidermatum[J].Physiological and Molecular Plant Pathology,2000,56(1):13-23.
[12]宋瑞芳,丁永乐,宫长荣,等.烟草抗病性与防御酶活性间的关系研究进展[J].中国农学通报,2007,23(5):309-314.
[13]VIDHYASEKARAN P.Host-specific toxin production by Rhizoctonia solani,the rice sheath blight pathogen[J].Phytopathology,1997,87(12):1258-1263.
[14]王光达,黄初女,吴委林,等.不同玉米品种对大斑病的抗性与相关防御酶活性的关系研究[J].玉米科学,2014,22(5):146-152.
[15]ZHANG Y,TANG HR,LUO Y.Variation in antioxidant enzyme activities of two strawberry cultivars with short term low temperature stress[J].World Journal of Agricultural Sciences,2008,4(4):458-462.
[16]李靖,利容千,哀文静.黄瓜感染霜霉病菌叶片中一些酶活性的变化[J].植物病理学报,1991,21(4):277-283.
[17]DROTAR A.Evidence for glutathione Peroxidase activity in cultured plant cells[J].Plant Science,1985,42:35-40.
[18]王爱国,罗广华.植物的超氧物自由基与轻胺反应的定量关系[J].植物生理学通讯,1990(6):55-57.
[19]吴样孙,陈一壮,蒙信满,等.水稻纹枯病抗性反应中主要防御酶的活性变化[J].中国农学通报,2008,24(5):327-330.
[20]左示敏,陈夕军,陈红旗,等.不同抗性水平水稻品种对纹枯病菌毒素的防卫反应与生理差异[J].中国水稻科学,2014,28(5):551-558.
[21]GUO SY,CHEN JH,XIAO Y,et al.Changes of physiological characteristics and yield in peanut varieties with different resistance effected by peanut scab[J].Agricultural Science and Technology,2015,16(10):2309-2313.
[22]MISHRA BB,GAUTAM S,SHARMA A,et al.Purification and characterisation of polyphenol oxidase(PPO)from eggplant(Solanum melongena)[J].Food Chemistry,2012,134(4):1855-1861.
[23]NIKRAFTAR F,TAHEIR P,RASTEGAR MF,et al.Tomato partial resistance to Rhizoctonia solani involves antioxidative defense mechanisms[J].Physiological and Molecular Plant Pathology,2013,81(4):74-83.