冰黄五苓散直肠灌注对缺血性脑卒中大鼠神经功能的影响
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摘要
目的:观察冰黄五苓散直肠灌注对缺血性脑卒中大鼠神经功能的影响。方法:将63只大鼠随机分为假手术组、模型组和治疗组,每组各21只。采用线栓法构建中动脉栓塞(middle artery embolism,MCAO)模型,假手术组和模型组给予9 ng·L~(-1)氯化钠注射液0.002 mL·g~(-1),加温至37℃直肠灌注。治疗组给予冰黄五苓散0.002 mL·g~(-1),加温至37℃直肠灌注。观察各组大鼠改良神经功能损害评分(modified neurological severity score,mNSS),干湿质量法测定脑组织含水量、脑组织HE染色及免疫组织染色观察神经元特异性烯醇化酶(neuronspecific enolase,NSE)及S-100β的表达情况。结果:模型组mNSS评分高于假手术组,差异具有统计学意义(P <0.05)。治疗组mNSS评分低于模型组,差异具有统计学意义(P <0.05)。假手术组与模型组、治疗组比较,脑水肿含量明显减少,差异具有统计学意义(P <0.05)。治疗组脑组织含水量低于模型组,差异具有统计学意义(P <0.05)。治疗组NSE的D10值明显高于模型组,S-100β的D10值明显低于模型组,差异均有统计学意义(P <0.05)。结论:冰黄五苓散对于大鼠缺血性卒中脑水肿模型具有减缓水肿发展和保护大脑神经功能的作用。
Objective: To observe the influence of Bing Huang Wuling Powder( BHWLP) for rectal perfusion on neurological function in rats with ischemic stroke.Methods: Sixty-three rats were randomly divided into the sham operation group,the model group and the control group,with 21 rats in each group.Middle artery embolism( MCAO) model was established by thread embolism.The rats in the sham operation group and the model group were given 9 ng·L~(-1)0.002 mL·g~(-1) of sodium chloride injection for rectal perfusion at a temperature of about 37 degrees Celsius.The ones in the treatment group were given 0.002 mL·g~(-1) of BHWLP for rectal perfusion at a temperature of about 37 degrees Celsius.Then each group's modified neurological severity score( mNSS) was observed.The water content in brain tissue was measured by dry-wet mass method.HE staining and immunohistochemical staining of Neuronspecific enolase( NSE) in brain tissue,and S-100 expression were also determined.Results: Compared with the model group and the treatment group,the mNSS score of the sham operation group significantly increased,and the difference was statistically significant( P < 0.05).The mNSS score of the treatment group was lower than that of the model group,and the difference was statistically significant( P < 0.05).Compared with the model group and the treatment group,the content of brain edema decreased significantly in the sham operation group,and the difference was statistically significant( P < 0.05).The water content of brain tissue in the treatment group was lower than that of the model group,and the difference was statistically significant( P < 0.05).The D10 value of NSE in the treatment group was significantly higher than that in the model group,and the D10 value of S-100 beta was significantly lower than that in the model group,and all the differences were statistically significant( P < 0.05).Conclusion: BHWLP has the effect of slowing down edema development and protecting cerebral nerve function in rats with ischemic stroke.
Objective: To observe the influence of Bing Huang Wuling Powder( BHWLP) for rectal perfusion on neurological function in rats with ischemic stroke.Methods: Sixty-three rats were randomly divided into the sham operation group,the model group and the control group,with 21 rats in each group.Middle artery embolism( MCAO) model was established by thread embolism.The rats in the sham operation group and the model group were given 9 ng·L~(-1)0.002 mL·g~(-1) of sodium chloride injection for rectal perfusion at a temperature of about 37 degrees Celsius.The ones in the treatment group were given 0.002 mL·g~(-1) of BHWLP for rectal perfusion at a temperature of about 37 degrees Celsius.Then each group's modified neurological severity score( mNSS) was observed.The water content in brain tissue was measured by dry-wet mass method.HE staining and immunohistochemical staining of Neuronspecific enolase( NSE) in brain tissue,and S-100 expression were also determined.Results: Compared with the model group and the treatment group,the mNSS score of the sham operation group significantly increased,and the difference was statistically significant( P < 0.05).The mNSS score of the treatment group was lower than that of the model group,and the difference was statistically significant( P < 0.05).Compared with the model group and the treatment group,the content of brain edema decreased significantly in the sham operation group,and the difference was statistically significant( P < 0.05).The water content of brain tissue in the treatment group was lower than that of the model group,and the difference was statistically significant( P < 0.05).The D10 value of NSE in the treatment group was significantly higher than that in the model group,and the D10 value of S-100 beta was significantly lower than that in the model group,and all the differences were statistically significant( P < 0.05).Conclusion: BHWLP has the effect of slowing down edema development and protecting cerebral nerve function in rats with ischemic stroke.
引文
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[18]代全德,司全春,徐忠海,等.急性脑出血患者血清炎症因子和氧化应激产物的动态监测及其临床意义[J].中华临床医师杂志(电子版),2014,8(3):39-43.
[19]张伟超,胡朝晖,郑永汉.血清S100β蛋白及IL-10含量预测急性脑出血患者迟发型脑水肿的临床意义[J].青岛医药卫生,2016,48(3):175-177.
[20]胡朝晖,郑永汉,张伟超.血清S100β蛋白及IL-10含量预测急性脑出血患者迟发性脑水肿的临床探析[J].黑龙江医药,2017,30(2):267-269.
[2]CHEN L,LIU Y,DONG L,et al.Edaravone protetcs human peripheral blood lymphocytes from gamma-irradiation-induced apoptosis and DNA damage[J].Cell Stress Chaperones,2015,20(2):289.
[3]ZHANG L,LU L,CHAN W M,et al.Effects of DL-3-n-butylphthalide on vascular dementia and angiogenesis[J].Neurochem Res,2012,37(5):911.
[4]石秦东,张军锋,张蓬勃,等.尤瑞克林对大鼠局灶性脑缺血半暗带脑血流量影响的研究[J].中国神经精神疾病杂志,2008,34(7):413.
[5]李金花.甘露醇不良反应的处理[J].内蒙古中医药,2013,32(34):74-75.
[6]杨金艳,彭兰,刘茂娟,等.栀子苷对大鼠脑缺血再灌注损伤后脑水肿的作用[J].中药药理与临床,2014,30(3):30-32.
[7]孙国柱,杨连琦,陈盼虎,等.灯盏花素对大鼠液压冲击伤性脑水肿水通道蛋白4表达的影响[J].中华中医药杂志,2013,28(12):3727-3729.
[8]黄海,黄丽霞.七叶皂苷钠针辅助治疗脑出血后继发脑水肿的临床观察[J].中国社区医师,2014,25(30):32-33.
[9]余尚贞,罗民新,艾立新,等.冰黄五苓液直肠滴注治疗急性脑出血76例疗效观察[J].新中医,2004,36(5):37-38.
[10]余尚贞,石青,艾立新,等.冰黄五苓液直肠滴注对急性脑出血患者内皮素水平的影响[J].中医杂志,2006,47(5):363-364.
[11]范瑞娟,罗亚非,孙玉凤.新式线栓法建立大鼠局灶性脑缺血再灌注模型的建立[J].中国民族民间医药,2014,23(2):26-27.
[12]ZEALONGA E L,WEINSTEIN R P,CARLSON S,et al.Reversible Middle Cerebral Artery Occlusion Without Craniectomy in Rats[J].Stroke,1989,20(1):84-91.
[13]赵雪松,陈志刚,高芳,等.三七总苷对脑出血大鼠凝血酶及神经功能的影响[J].世界中医药,2013,8(11):1335-1337.
[14]李强,王爱岳,刘运生.HIF-1α在脑出血灶周的表达及其脑水肿的关系[J].中国耳鼻咽喉颅底外科杂志,2007,13(3):161-163.
[15]朱亮,顾文政,余尚贞.冰黄五苓散直肠滴注对缺血性脑卒中大鼠脑组织S100β、NSE表达的影响[J].广州中医药大学学报,2017,34(1):81-85.
[16]RIGHINI M,BOUNAMEAUX H.Risk factors and management of deep vein thrombosis[J].Herz,2007,32(1):27.
[17]WUNDERLICH M T,EBERT A D,KRATZ T,et al.Early neurobehavioral outcome after stoke is related to release of neurobiochemical marker of brain damage[J].Stroke,1990,30(6):1190-1195.
[18]代全德,司全春,徐忠海,等.急性脑出血患者血清炎症因子和氧化应激产物的动态监测及其临床意义[J].中华临床医师杂志(电子版),2014,8(3):39-43.
[19]张伟超,胡朝晖,郑永汉.血清S100β蛋白及IL-10含量预测急性脑出血患者迟发型脑水肿的临床意义[J].青岛医药卫生,2016,48(3):175-177.
[20]胡朝晖,郑永汉,张伟超.血清S100β蛋白及IL-10含量预测急性脑出血患者迟发性脑水肿的临床探析[J].黑龙江医药,2017,30(2):267-269.