马尾松种子园交配系统的时间动态变异
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摘要
以马尾松种子园不同时期(建园初期、丰产期及衰老期)产出的子代为材料,采用SSR标记分析其遗传多样性,并结合子代测定数据分析交配系统对子代遗传品质的影响。研究结果表明,建园初期与丰产期的子代家系的Shannon多样性指数(I)较高,同为0.53,衰老期的子代家系则为0.47。观测杂合度(Ho)和期望杂合度(He)均以建园初期子代群体为最高,丰产期子代次之,衰老期最低。马尾松种子园建园初期与丰产期的子代均具有较高的遗传多样性。丰产期参与繁殖的父本数量最多,近交水平较低,从而保证了种子的品质。而种子园衰老期子代的遗传多样性明显下降,说明种子园的老化不仅影响种子产量,而且影响种子遗传品质。在亲本分析中,种子园建园初期、丰产期及衰老期的子代均未检测到自交情况。由此可进一步证实马尾松是典型的异交树种。
We studied the influence of mating system to the quality of the offspring which was based on the Masson pine seed orchard from different period(at the beginning period,high yield period and aging period),SSR markers for genetic diversity and progeny test data of offspring.At the beginning and high yield period,the offspring Shannon diversity index(I)was high,both values were 0.53,and at the aging period was 0.47.The observed heterozygosity(Ho)and expected heterozygosity(He)of the offspring population was highest at the beginning period followed by high yield period,and the aging period was the lowest.Offspring from the beginning period and high yield period of seed orchard of Masson pine had high genetic diversity.In high yield period,the number of male reproductive participation was the largest,and inbreeding level was low,so as to ensure the quality of the seeds.The genetic diversity of offspring was significantly decreased at the aging period,which meant that the aging of seed orchard affected the seed yield and the genetic quality of the seed.In the parent analysis,inbreeding was not detected in offspring at the beginning,high yield and aging period.The Masson pine is a typical outcrossing species.
We studied the influence of mating system to the quality of the offspring which was based on the Masson pine seed orchard from different period(at the beginning period,high yield period and aging period),SSR markers for genetic diversity and progeny test data of offspring.At the beginning and high yield period,the offspring Shannon diversity index(I)was high,both values were 0.53,and at the aging period was 0.47.The observed heterozygosity(Ho)and expected heterozygosity(He)of the offspring population was highest at the beginning period followed by high yield period,and the aging period was the lowest.Offspring from the beginning period and high yield period of seed orchard of Masson pine had high genetic diversity.In high yield period,the number of male reproductive participation was the largest,and inbreeding level was low,so as to ensure the quality of the seeds.The genetic diversity of offspring was significantly decreased at the aging period,which meant that the aging of seed orchard affected the seed yield and the genetic quality of the seed.In the parent analysis,inbreeding was not detected in offspring at the beginning,high yield and aging period.The Masson pine is a typical outcrossing species.
引文
[1]DONALD L C.The influence of floral bud phenology on the potential mating systemof awind-pollinated Douglas-fir orchard[J].Can J For Res,1991,21(6):813-820.
[2]GIOVANN D F,KEVAN P G.Factors affecting pollen dynamics and its importance to pollen contamination are view[J].Can J For Res,1991,21(8):1155-1170.
[3]张华新,沈熙环.林木种子园生殖系统研究进展[J].林业科学,2002,38(2):129-134.
[4]OZAWA H,WATANABE A,UCHIYAMA K,et al.Genetic diversity of Pinus densiflora pollen flowing over fragmented populations during a mating season[J].Journal of Forest Research,2012,17(6):488-498.
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[7]龚佳.马尾松实生种子园遗传多样性研究[D].南京:南京林业大学,2007.
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[14]FENG Y H,YANG Z Q,WANG J,et al.Development and characterization of SSR markers from Pinus massoniana and their transferability to P.elliottii,P.caribaea and P.yunnanensis[J].Genetics&Molecular Research,2013,13(1):1508-1513.
[15]杨章旗,冯源恒,吴东山.细叶云南松天然种源林遗传多样性的SSR分析[J].广西植物,2014,4(1):10-14.
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[21]何田华,葛颂.植物种群交配系统、亲本分析以及基因流动研究[J].植物生态学报,2001,25(2):144-154.
[22]杨章旗,韦元荣.马尾松无性系嫁接种子园营建技术研究[J].广西林业科学,2001,30(2):56-61.
[23]艾畅,徐立安,赖焕林,等.马尾松种子园的遗传多样性与父本分析[J].林业科学,2006,42(11):146-150.
[24]谭晓梅.马尾松二代育种亲本选择及种子园交配系统研究[D].北京:中国林业科学研究院,2011.
[25]王鹏良.马尾松无性系种子园多年份子代遗传多样性分析[D].南京:南京林业大学,2006.
[26]WHEELER N C,JECH K S.The use of electrophoretic markers in seed orchard research[J].New Forests,1992,6(2):311-328.
[27]KASSABY Y A,PARKINSON J,DAVITT W J B.The effect of crown segment on the mating system in a Douglas-fir(Pseudotsuga menziesii(Mirb)Franco)seed orchard[J].Silvae Genetica,1986,35(4):149-155.
[2]GIOVANN D F,KEVAN P G.Factors affecting pollen dynamics and its importance to pollen contamination are view[J].Can J For Res,1991,21(8):1155-1170.
[3]张华新,沈熙环.林木种子园生殖系统研究进展[J].林业科学,2002,38(2):129-134.
[4]OZAWA H,WATANABE A,UCHIYAMA K,et al.Genetic diversity of Pinus densiflora pollen flowing over fragmented populations during a mating season[J].Journal of Forest Research,2012,17(6):488-498.
[5]赖焕林,王章荣.马尾松种子园及其附近人工林的亲子代群体遗传结构分析[J].林业科学研究,1997,10(5):490-494.
[6]张小琴.马尾松1.5代无性系种子园花量调查与遗传多样性分析[D].南京:南京林业大学,2008.
[7]龚佳.马尾松实生种子园遗传多样性研究[D].南京:南京林业大学,2007.
[8]张薇,龚佳,季孔庶.马尾松实生种子园交配系统分析[J].林业科学,2009,45(6):22-26.
[9]谭小梅,周志春,金国庆,等.马尾松二代无性系种子园遗传多样性和交配系统分析[J].林业科学,2012,48(2):69-74.
[10]覃开展,罗筱娥.马尾松种子园无性系生长结实规律研究[J].广西林业科学,2001,30(1):28-31.
[11]谭健晖.马尾松种子园无性系开花习性研究[J].广西林业科学,2001,30(2):76-78.
[12]杨章旗,黄永利,覃开展,等.马尾松速生良种‘广西南宁市林科所马尾松初级无性系种子园种子’[J].林业科学,2014,50(3):151-152.
[13]DOYLE J J,DOYLE J L.Isolation of plant DNA from fresh tissue[J].Focus,1990,12:13-15.
[14]FENG Y H,YANG Z Q,WANG J,et al.Development and characterization of SSR markers from Pinus massoniana and their transferability to P.elliottii,P.caribaea and P.yunnanensis[J].Genetics&Molecular Research,2013,13(1):1508-1513.
[15]杨章旗,冯源恒,吴东山.细叶云南松天然种源林遗传多样性的SSR分析[J].广西植物,2014,4(1):10-14.
[16]YEH F C,YANG R C,BOYLE T B,et al.The user-friendly shareware for population genetic analysis.Molecular biology and biotechnology centre[M].Edmonton:University of Alberta Canada,1997.
[17]HARTL D L,CLARK A G.Principles of population genetics,ed3[J].Sinauer Associates,1997,4(2):143-144.
[18]SHANNON C E,WEAVER W.The Mathematical Theory of Communication[M].Urbana:University of Illinois Press,1949.
[19]NEI M.Analysis of gene diversity in subdivided populations[J].Proceedings of the National Academy of Sciences,1974,70(12):3321-3323.
[20]MARSHALL T C,SLATE J,KRUUK L E B,et al.Statistical confidence for likelihood-based paternity inference in natural populations[J].Molecular Ecology,1998,7(5):639-655.
[21]何田华,葛颂.植物种群交配系统、亲本分析以及基因流动研究[J].植物生态学报,2001,25(2):144-154.
[22]杨章旗,韦元荣.马尾松无性系嫁接种子园营建技术研究[J].广西林业科学,2001,30(2):56-61.
[23]艾畅,徐立安,赖焕林,等.马尾松种子园的遗传多样性与父本分析[J].林业科学,2006,42(11):146-150.
[24]谭晓梅.马尾松二代育种亲本选择及种子园交配系统研究[D].北京:中国林业科学研究院,2011.
[25]王鹏良.马尾松无性系种子园多年份子代遗传多样性分析[D].南京:南京林业大学,2006.
[26]WHEELER N C,JECH K S.The use of electrophoretic markers in seed orchard research[J].New Forests,1992,6(2):311-328.
[27]KASSABY Y A,PARKINSON J,DAVITT W J B.The effect of crown segment on the mating system in a Douglas-fir(Pseudotsuga menziesii(Mirb)Franco)seed orchard[J].Silvae Genetica,1986,35(4):149-155.