黑曲发酵茯苓多糖免疫调节作用研究
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摘要
研究黑曲发酵茯苓多糖对免疫抑制小鼠的免疫调节作用,通过构建免疫抑制小鼠模型,将小鼠随机分为4组,即空白对照组(CK)、环磷酰胺模型组(cyclophosphamide,CY)及发酵茯苓多糖(fermented Poria cocos polysaccharides,FPCP)高、低剂量组。小鼠以灌胃方式给予不同剂量发酵茯苓多糖,分别测定脏器指数、血清溶血素水平、免疫球蛋白G(immunoglobulin G,IgG)与免疫球蛋白M(immunoglobulin M,IgM)含量及单核-巨噬细胞吞噬率。结果表明,与模型组相比,发酵茯苓多糖各剂量组均能显著提高小鼠脏器指数、溶血素水平、IgG与IgM含量、单核-巨噬细胞吞噬率。发酵茯苓多糖能有效增强环磷酰胺致免疫低下小鼠的免疫调节作用。
To study the immunoregulatory effect of Aspergillus niger fermentation polysaccharide on immunosuppressive mice,immunosuppressed mouse model was constructed. The mice were randomly divided into 4 groups:control check(CK),cyclophosphamide model group(CY),high-dose and low-dose groups of fermented Poria cocos polysaccharides(FPCP). The mice were given different doses of fermented polysacc-harides by gavage,and the organ index,serum hemolysin level,immunoglobulin G(IgG)and immunoglobulin M(IgM)content,macrophage phagocytosis rate were measured. The results showed that compared with the model group(CY),each dose of FPCP significantly increased the mouse organ index,hemolysin level,IgG and IgM content,and macrophage phagocytosis rate. The polysaccharides fermented by Aspergillus niger can effectively enhance the immunoregulatory effect of immunocompromised mice.
To study the immunoregulatory effect of Aspergillus niger fermentation polysaccharide on immunosuppressive mice,immunosuppressed mouse model was constructed. The mice were randomly divided into 4 groups:control check(CK),cyclophosphamide model group(CY),high-dose and low-dose groups of fermented Poria cocos polysaccharides(FPCP). The mice were given different doses of fermented polysacc-harides by gavage,and the organ index,serum hemolysin level,immunoglobulin G(IgG)and immunoglobulin M(IgM)content,macrophage phagocytosis rate were measured. The results showed that compared with the model group(CY),each dose of FPCP significantly increased the mouse organ index,hemolysin level,IgG and IgM content,and macrophage phagocytosis rate. The polysaccharides fermented by Aspergillus niger can effectively enhance the immunoregulatory effect of immunocompromised mice.
引文
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[3]陈林,廖彦,黄威,等.茯苓胞外多糖、胞内多糖与菌核多糖的抗氧化活性比较研究[J].北方园艺,2018(2):172-177
[4]熊芳琪,刘欣,杨岚,等.羧甲基茯苓多糖体外抗氧化活性研究[J].中国食物与营养,2017,23(7):39-41
[5]张卫元,杨阳,陈翠兰,等.茯苓散对免疫低下小鼠免疫功能的影响[J].中国兽医学报,2014,34(2):283-287
[6]侯玮婷,罗佳波.复方茯苓多糖口服液抗肿瘤作用和免疫调节功能的初步研究[J].中药药理与临床,2017(2):78-81
[7]张秀军,徐俭,林志彬,等.羧甲基茯苓多糖对小鼠免疫功能的影响[J].中国药学杂志,2002,37(12):913-916
[8]李慧.茯苓多糖的改性及其体外抗氧化活性评价[D].武汉:华中农业大学,2013:2-26
[9]林标声,陈小红,罗茂春,等.高取代度羧甲基茯苓多糖(CMP)的制备及其注射剂的研制[J].中国农学通报,2015,31(29):59-64
[10]武洪志,夏九龙,王芳芳,等.黑曲霉发酵茯苓添加剂的工艺优化[J].中国畜牧杂志,2016,52(3):60-66
[11]黄灿,王玉明,赵骏,等.抗肿瘤活性茯苓多糖的提取、纯化与结构分析[J].中草药,2012,43(11):2146-2149
[12]彭小彬,邱小惠,余传林,等.茯苓多糖对环磷酰胺所致免疫功能低下小鼠体液免疫功能的影响[J].中药药理与临床,2013(5):69-72
[13]Scott J M,Lebratti T J,Richner J M,et al.Cellular and Humoral Immunity Protect against Vaginal Zika Virus Infection in Mice[J].Journal of Virology,2018,92(7):8-18
[14]胡明华,邓向亮,徐路,等.香菇茯苓银耳复合多糖对小鼠巨噬细胞功能的作用研究[J].中国免疫学杂志,2018(1):44-49
[15]Chai Y H,Zhang Y C.Analysis on monosaccharide composition of polysaccharides in Ziziphus jujube cv.Lingwuchangzao from different areas in Ningxia[J].Guihaia,2017,37(9):1187-1194
[16]高笑笑,王刚,杨歆睿,等.雪灵芝粗多糖对环磷酰胺致免疫抑制小鼠免疫功能的影响[J].免疫学杂志,2017(3):191-196