摘要
目的:探讨延寿液通过调控炎症和维持胶原延缓臭氧吸入所诱导大鼠皮肤衰老的作用机制。方法:将48只大鼠按照随机数字表法分为对照组、模型组、维生素E组、延寿液低剂量组、延寿液中剂量组和延寿液高剂量组,每组各8只。对照组大鼠每天吸入新鲜空气10 h,模型组和各给药组大鼠每天吸入臭氧(1. 2±0. 2) ppm,10 h造模。检测各组大鼠血浆/肝肾组织总超氧化物歧化酶(T-SOD)和铜锌超氧化物歧化酶(CuZn-SOD)活力、血浆锰超氧化物歧化酶(MnSOD)活力,血清/肝肾组织丙二醛(malondialdeyde,MDA)含量,皮肤羟脯氨酸(Hyp)含量,苦味酸天狼星红染色定量皮肤胶原面积,免疫组织化学染色检测转化生长因子-β(transforming growth factor-β,TGF-β)表达,蛋白斑点测定皮肤基质金属蛋白酶-1(matrix metalloproteinase-1,MMP-1)表达变化。结果:与对照组比较,模型组大鼠血液T-SOD、MnSOD的活性及Hyp含量下降,MDA含量显著升高(P <0. 01)。与模型组比较,延寿液低剂量组、延寿液中剂量组和延寿液高剂量组T-SOD、Mn-SOD的活性显著升高,CuZn-SOD活性、MDA及Hyp含量显著降低,差异具有统计学意义(P <0. 01)。与对照组比较,模型组肝组织MDA含量升高,肾组织T-SOD、CuZn-SOD活力降低,MDA含量明显增高(P <0. 01);与模型组比较,延寿液低剂量组肝MDA含量降低显著(P <0. 01),延寿液低剂量组、中剂量组肾T-SOD活力显著升高(P <0. 05),延寿液低剂量组肾MDA的含量显著降低(P <0. 05);与对照组比较,模型组TGF-β1含量降低、皮肤MMP-1蛋白表达显著增高,差异有统计学意义(P <0. 01);与模型组比较,维生素E组和延寿液低剂量组中TGF-β1含量升高、皮肤MMP-1的表达降低,差异具有统计学意义(P <0. 05)。结论:延寿液通过上调TGF-β1调控炎症,同时下调MMP-1维持胶原,具有延缓皮肤衰老的作用。
Objective: To explore the mechanism of Yanshou Decoction( YSD) in delaying skin aging in rats by regulating inflammation and maintaining collagen. Methods: Forty-eight rats were randomly divided into the control group,the model group,the vitamin E group,low-dose YSD group,high-dose YSD group and high-dose YSD group,with 8 rats in each group. The rats in the control group were inhaled fresh air for 10 hours every day,and the rats in the model group and the administration groups were inhaled ozone( 1. 2 ± 0. 2) ppm every day for 10 hours. The change of total superoxide dismutase( T-SOD) in plasma/liver and kidney tissues,activity of copper-zinc superoxide dismutase( Cu Zn-SOD),the activity of plasma manganese superoxide dismutase( Mn-SOD),the content of malondialdehyde( MDA) in serum/liver and kidney tissue,the skin hydroxyproline( Hyp) content,quantitative collagen area of skin with Sirius picrate red staining in rats of each group were detected. The expression of transforming growth factor-beta( TGF-β) was detected by immunohistochemical staining and the expression of matrix metalloproteinase-1( MMP-1) in skin was detected by protein spot assay. Results: Compared with the control group,the activity of T-SOD,Mn-SOD and the content of Hyp in the blood of the model group's rats decreased,while the content of MDA increased significantly( P <0. 01). Compared with model group,the activities of T-SOD and Mn-SOD in low dose group,high dose group and high dose group of YSD increased significantly( P < 0. 01),while their activities of Cu Zn-SOD,MDA and Hyp decreased significantly,and all the differences were statistically significant( P < 0. 01). Compared with the control group,MDA content in liver tissue increased,while T-SOD and Cu Zn-SOD activity in kidney tissue decreased and MDA content increased significantly in the model group( P <0. 01). Compared with the model group,the MDA content in liver decreased significantly( P < 0. 01),while the T-SOD activity in kidney increased significantly( P < 0. 05) and the MDA content in kidney decreased significantly( P < 0. 05) in the low-dose group and the medium-dose group of YSD. Compared with the control group,the content of TGF-β1 decreased and the expression of MMP-1 protein increased significantly in the model group,and all the differences were statistically significant( P < 0. 01).Compared with the model group,the content of TGF-β1 increased and the expression of MMP-1 decreased in the vitamin E group and the low-dose YSD group and all the differences were statistically significant( P < 0. 05). Conclusion: YSD has the effect of delaying skin aging by up-regulating TGF-β1 and down-regulating MMP-1 to maintain collagen.
引文
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