粗壮女贞对持续性干旱及复水的生理响应
|
摘要
为了探讨粗壮女贞(Ligustrum robustum)幼苗对持续干旱胁迫的适应能力、恢复能力及生理响应特征,采用温室盆栽方法模拟土壤干旱条件,分别对干旱胁迫5、10、15、20和25 d及胁迫后复水5、10、20 d的幼苗渗透调节物质含量、叶片相对电导率(REC)、水分饱和亏(WSD)、丙二醛(MDA)含量及保护酶活性的变化规律进行研究。结果表明:干旱胁迫对粗壮女贞叶片生理指标有显著影响,复水后,各指标均有向正常水平恢复的趋势。随着干旱时间的延长,粗壮女贞叶片脯氨酸(Pro)含量、REC、WSD等指标呈增加趋势;可溶性蛋白质含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性呈先升后降的变化,但两种保护酶活性增幅和响应时间不同,表现出不同的清除过氧化物功能;MDA含量呈"升-降-升"的波浪式趋势。干旱胁迫的程度不同,幼苗复水后各指标恢复能力和恢复程度各异,干旱时间越长,恢复需要的时间也越长,各指标恢复时间与干旱程度呈正相关。综合表明,土壤相对含水量为32.51%是粗壮女贞正常生长所需最低土壤含水量。
To investigate adaptive ability, recovering ability and physiological characters of Ligustrum robustum seedlings to continuous drought stress, greenhouse-pot culture method was adopted to simulate soil drought conditions, and analyze variation patterns in content of osmotic adjustment substances, leaf relative electrical conductivity(REC), water saturation deficit(WSD), malondialdehyde(MDA) content and protective enzyme activities of seedlings after exposure to drought stress(for 5, 10, 15, 20 and 25 days) and rewatering(5, 10 and 15 days) treatments. Results show that drought stress had significant influences on physiological indexes of leaves of L. robustum, and all indexes tended to recover towards normal levels after being rewatered. As drought stress prolonged, proline(Pro) content, REC and WSD of L. robustum increased; soluble protein content and activities of SOD and POD first increased and then decreased, but the degree of increase and response time differed, exhibiting different functions of peroxide scavenging; MDA content tended to increase, decrease and re-increase. As drought stress continued, the recovering ability and extent of various indexes were different after seedlings were rewatered. Their recovery time increased as drought lasted longer, and recovery time of indexes positively correlated with the extent of drought stress. The 32.51% of soil relative water content was the threshold for the normal growth of L. robustum.
To investigate adaptive ability, recovering ability and physiological characters of Ligustrum robustum seedlings to continuous drought stress, greenhouse-pot culture method was adopted to simulate soil drought conditions, and analyze variation patterns in content of osmotic adjustment substances, leaf relative electrical conductivity(REC), water saturation deficit(WSD), malondialdehyde(MDA) content and protective enzyme activities of seedlings after exposure to drought stress(for 5, 10, 15, 20 and 25 days) and rewatering(5, 10 and 15 days) treatments. Results show that drought stress had significant influences on physiological indexes of leaves of L. robustum, and all indexes tended to recover towards normal levels after being rewatered. As drought stress prolonged, proline(Pro) content, REC and WSD of L. robustum increased; soluble protein content and activities of SOD and POD first increased and then decreased, but the degree of increase and response time differed, exhibiting different functions of peroxide scavenging; MDA content tended to increase, decrease and re-increase. As drought stress continued, the recovering ability and extent of various indexes were different after seedlings were rewatered. Their recovery time increased as drought lasted longer, and recovery time of indexes positively correlated with the extent of drought stress. The 32.51% of soil relative water content was the threshold for the normal growth of L. robustum.
引文
An Y,Liang Z,Hao W(2011).Growth and physiological responses of the Periploca sepium Bunge seedlings to drougut stress.Acta Ecol Sin,31(3):716-725(in Chinese with English abstract)[安玉艳,梁宗锁,郝文芳(2011).杠柳幼苗对不同强度干旱胁迫的生长与生理响应.生态学报,31(3):716-725]
Arakawa T,Timasheff SN(1985).The stabilization of proteins by osmolytes.Biophys J,47(3):411-414
Cai Y(2014).Theoretical and Experimental Plant Physiology.Beijing:China Agricultural University Press,121-129(in Chinese)[蔡永萍(2014).植物生理学实验指导.北京:中国农业大学出版社,121-129]
Cao J,Jiang W,Zhao Y(2006).Theoretical and Experimental Guidance in Fruit and Vegetables Postharvest Physiology.Beijing:China Light Industry Press,68-70(in Chinese)[曹建康,姜微波,赵玉梅(2006).果蔬采后生理生化实验指导.北京:中国轻工业出版社,68-70]
Fan SL,Yuan ZL,Feng LJ,et al(2011).Effects of drought stress on physiological and biochemical parameters of Dahlia pinnata.Chin J App Ecol,22(3):651-657(in Chinese with English abstract)[范苏鲁,苑兆和,冯立娟等(2011).干旱胁迫对大丽花生理生化指标的影响.应用生态学报,22(3):651-657]
Hao JJ,Kang ZL,Yu Y(2007).Experimental Techniques of Plant Physiology.Beijing:Chemical Industry Press(in Chinese)[郝建军,康宗利,于洋(2007).植物生理学实验技术.北京:化学工业出版社]
Hong Z,Lian F,Liu S,et al(2016).Physiological response of three native garden ground cover plant seedings to increasing drought stress.J Zhejiang A&F Univ,33(4):636-642(in Chinese with English abstract)[洪震,练发良,刘术新等(2016).3种乡土园林地被植物对干旱胁迫的生理响应.浙江农林大学学报,33(4):636-642]
Hsiao TC(1973).Plant response to water stress.Ann Rev Plant Physiol,24:519-570
Kang JM,Yang QC,Fan FC(2005).Effects of drought stress on induced protein in the different drought resistance alfalfa leaf.Acta Agrestia Sin,13(3):199-202(in Chinese with English abstract)[康俊梅,杨青川,樊奋成(2005).干旱对苜蓿叶片可溶性蛋白的影响.草地学报,13(3):199-202]
Li L,Liu Y,Wang M,et al(2014).Physiological response mechanism of three kinds of Acer rubrum L.under continuous high temperature and drought stress.Acta Ecol Sin,34(22):6471-6480(in Chinese with English abstract)[李力,刘玉民,王敏等(2014).3种北美红枫对持续高温干旱胁迫的生理响应机制.生态学报,34(22):6471-6480]
Li Y,Sun MG,King YJ,et al(2006).Physiological and biochemical responses of Gleditsia sinensis seedlings to drought stress.J South China Agr Univ,27(3):66-69(in Chinese with English abstract)[李燕,孙明高,孔艳菊等(2006).皂角苗木对干旱胁迫的生理生化反应.华南农业大学学报,27(3):66-69]
Liu G,Li X,Liang Z,et al(2003).Technoiques of tissue culture and in vitro clonal propagation of Ilex latifolia.For Res,16(4):439-443(in Chinese)[刘根林,李晓储,梁珍海等(2003).优良保健树大叶冬青组培扩繁的研究.林业科学研究,16(4):439-443]
Liu HY,Liang ZS,Liu SM,et al(2007).Effect of progressive drying and rewatering on protective enzym activities and osmoregulatory molecules in leaves of Eucommia ulmoides seedling.J Northwest For Univ,22(3):55-59(in Chinese with English abstract)[刘红云,梁宗锁,刘淑明等(2007).持续干旱及复水对杜仲幼苗保护酶活性和渗透调节物质的影响.西北林学院学报,22(3):55-59]
Liu J,Zhu JK(1997).Proline accumulation and salt-stressinduced gene expression in a salt-hypersensitive mutant of Arabidopsis.Plant Physiol,114(2):591-596
Liu S,Qin Y,Du F(2003).Studies on chemical components in leaf of Ilex latifolia.China J Chin Mater Med,28(9):44-46(in Chinese)[刘韶,秦勇,杜方麓(2003).苦丁茶化学成分研究.中国中药杂志,28(9):44-46]
Lu S,Chen S,Chen S,et al(2003).Responses of proline content and activities of enzymes in warm-season turfgrasses to soil drought stress.Acta Hortic Sin,30(3):303-306(in Chinese)[卢少云,陈斯平,陈斯曼等(2003).三种暖季型草坪草在干旱条件下脯氨酸含量和抗氧化酶活性的变化.园艺学报,30(3):303-306]
Luo YL,Bi TJ,Su ZL,et al(2014).Physiological response of Kalanchoe tubiflora leaves to drogut stress and rewatering.J Trop Subtrop Bot,22(4):391-398(in Chinese with English abstract)[罗银玲,毕廷菊,苏志龙等(2014).棒叶落地生根对干旱与复水的生理响应.热带亚热带植物学报,22(4):391-398]
Pan X,Qiu Q,Li J,et al(2014).Physiological indexes of six plant species from the tibetan plateau under drought stress.Acta Ecol Sin,34(13):3558-3567(in Chinese with English abstract)[潘昕,邱权,李吉跃等(2014).干旱胁迫对青藏高原6种植物生理指标的影响.生态学报,34(13):3558-3567]
Seo HL,Baek SY,Lee EH,et al(2017).Liqustri lucidi fructus inhibits hepatic injury and functions as an antioxidant by activation of AMP-activated protein kinase in vivo and in vitro.Chemico-Biol Interact,262:57-68
Su H,Jin J,Zhu Z(2014).Effects of drought stress on physiological characteristics of Quercus rubra seedlings.JNortheast For Univ,42(8):34-39(in Chinese with English abstract)[苏寒之,金建邦,祝遵凌(2014).干旱胁迫对北美红栎幼苗生理特性的影响.东北林业大学学报,42(8):34-39]
Tang ZC(1983).Plant responses and adaptability to water stress.Plant Physiol Commun,(3):24-29(in Chinese with English abstract)[汤章城(1983).植物对水分胁迫的反应和适应性.I.抗逆性的一般概念和植物的抗涝性.植物生理学通讯,(3):24-29]
Tang ZC(1984).Plant proline accumulation and its meaning in stress condition.Plant Physiol Commun,(1):15-21(in Chinese with English abstract)[汤章城(1984).逆境条件下植物脯氨酸的累积及其可能的意义.植物生理学通讯,(1):15-21]
Wang BS(1988).Biological free radicals and membrane damage of plants.Plant Physiol Commun,(2):12-16(in Chinese with English abstract)[王宝山(1988).生物自由基与植物膜伤害.植物生理学通讯,(2):12-16]
Wang BX,Huang JC,Wang H,et al(1989).The correlation of proline accumulation and drought resistance in various plants under water stress condition.Plant Physiol J,(1):46-51(in Chinese with English abstract)[王邦锡,黄久常,王辉等(1989).不同植物在水分胁迫条件下脯氨酸的累积与抗旱性的关系.植物生理学报,(1):46-51]
Wang L,Wu J,Xia PY,et al(2011).Physiological responses of Ilex latifolia to low temperature and its cold tolerance.J South China Agr Univ,32(3):82-86(in Chinese with English abstract)[王宁,吴军,夏鹏云等(2011).大叶冬青对低温胁迫的生理响应及抗寒性分析.华南农业大学学报,32(3):82-86]
Wang QM,Xu XC,Wu SG,et al(2005).The effect of drought stress on the content of osmotic adjusting substance in leaves and cell membrane permeability of different soybean varieties in seedling period.Seed,24(8):9-12(in Chinese with English abstract)[王启明,徐心诚,吴诗光等(2005).干旱胁迫对不同大豆品种苗期叶片渗透调节物质含量和细胞膜透性的影响.种子,24(8):9-12]
Wang X,Huang J(2015).Principles and Techniques of Plant Physiological and Biochemical Experiments.3rd ed.Beijing:Higher Education Press,274-277(in Chinese)[王学奎,黄见良(2015).植物生理生化实验原理与技术.第3版.北京:高等教育出版社,274-277]
Wu Y,Guo S(2017).Physiological response of Chinese varieties of drought stress and evaluation of drought resistance.J Northeast For Univ,45(1):20-24,29(in Chinese with English abstract)[武燕奇,郭素娟(2017).5个板栗品种对干旱胁迫的生理响应及抗旱性评价.东北林业大学学报,45(1):20-24,29]
Xiao WJ,Yang WL,Gong ZH(2000).Processing techniques of fresh leaves of Ilex kudingcha C.J.Tseng.J Hunan Agr Univ,26(4):278-281(in Chinese with English abstract)[肖文军,杨伟丽,龚志华(2000).苦丁茶鲜叶加工技术研究.湖南农业大学学报,26(4):278-281]
Xie M,Wang Q,Xu D,et al(2015).Comparative study of the effects on colonic microbiota fermentation in vitro of extracts from Ilex kudingcha C.J.Tseng and 3,5-dicaffeoylquinic acid.J Food Sci,36(17):124-129(in Chinese with Englishabstract)[谢旻皓,王晴川,徐冬兰等(2015).苦丁茶冬青苦丁茶提取物与3,5-双咖啡酰奎尼酸对肠道微生物体外发酵的影响.食品科学,36(17):124-129]
Ye Q,Zuo HJ,Liao HY,et al(2013).Study on in vitro antimicrobial activity of L.purpurascents Y.C.Yang.Mod Prev Med,40(7):1320-1322(in Chinese with English abstract)[叶倩,左浩江,廖虹瑜等(2013).粗壮女贞提取物的体外抑菌作用研究.现代预防医学,40(7):1320-1322]
Yi F,Zhao XL,Peng Y,et al(2016).Genus llex L.:phytochemistry,ethnopharmacology,and pharmacology.Chin Herb Med,8(3):209-230
Zhang L,Wei L,Long P,et al(2017).Response of Trachycarpus fortunei seedlings from different provenances to drought stress and drought resistance evaluation.J Northeast For Univ,45(11):1-5(in Chinese with English abstract)[张兰,韦小丽,龙鹏等(2017).不同种源棕榈幼苗对干旱胁迫的响应及抗旱性评价.东北林业大学学报,45(11):1-5]
Zhang L,Wu J,Mei L,et al(2011).Saline tolerance of Camptotheca acuminata provenances and the index selection for saline tolerance.Sci Silvae Sin,47(11):66-72(in Chinese with English abstract)[张露婷,吴江,梅丽等(2011).喜树种源耐盐能力评价及耐盐指标筛选.林业科学,47(11):66-72]
Zhang MS,Xie B,Tan F,et al(2003).Relationship among soluble protein,chlorophyll and ATP in sweet potato under water stress with drought resistance.Sci Agric Sin,36(1):13-16(in Chinese with English abstract)[张明生,谢波,谈锋等(2003).甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究.中国农业科学,36(1):13-16]
Zhang WT,Xie FC,Wang HT,et al(2009).Physiological response of three garden plants to drought stress.J Zhejiang For Coll,26(2):182-187(in Chinese with English abstract)[张文婷,谢福春,王华田等(2009).3种园林灌木幼苗对干旱胁迫的生理响应.浙江林学院学报,26(2):182-187]
Zhang YT(1994).Origin of kudingcha.J Syst Evol,32(1):100-102(in Chinese with English abstract)[张永田(1994).苦丁茶的原植物.植物分类学报,32(1):100-102]
Arakawa T,Timasheff SN(1985).The stabilization of proteins by osmolytes.Biophys J,47(3):411-414
Cai Y(2014).Theoretical and Experimental Plant Physiology.Beijing:China Agricultural University Press,121-129(in Chinese)[蔡永萍(2014).植物生理学实验指导.北京:中国农业大学出版社,121-129]
Cao J,Jiang W,Zhao Y(2006).Theoretical and Experimental Guidance in Fruit and Vegetables Postharvest Physiology.Beijing:China Light Industry Press,68-70(in Chinese)[曹建康,姜微波,赵玉梅(2006).果蔬采后生理生化实验指导.北京:中国轻工业出版社,68-70]
Fan SL,Yuan ZL,Feng LJ,et al(2011).Effects of drought stress on physiological and biochemical parameters of Dahlia pinnata.Chin J App Ecol,22(3):651-657(in Chinese with English abstract)[范苏鲁,苑兆和,冯立娟等(2011).干旱胁迫对大丽花生理生化指标的影响.应用生态学报,22(3):651-657]
Hao JJ,Kang ZL,Yu Y(2007).Experimental Techniques of Plant Physiology.Beijing:Chemical Industry Press(in Chinese)[郝建军,康宗利,于洋(2007).植物生理学实验技术.北京:化学工业出版社]
Hong Z,Lian F,Liu S,et al(2016).Physiological response of three native garden ground cover plant seedings to increasing drought stress.J Zhejiang A&F Univ,33(4):636-642(in Chinese with English abstract)[洪震,练发良,刘术新等(2016).3种乡土园林地被植物对干旱胁迫的生理响应.浙江农林大学学报,33(4):636-642]
Hsiao TC(1973).Plant response to water stress.Ann Rev Plant Physiol,24:519-570
Kang JM,Yang QC,Fan FC(2005).Effects of drought stress on induced protein in the different drought resistance alfalfa leaf.Acta Agrestia Sin,13(3):199-202(in Chinese with English abstract)[康俊梅,杨青川,樊奋成(2005).干旱对苜蓿叶片可溶性蛋白的影响.草地学报,13(3):199-202]
Li L,Liu Y,Wang M,et al(2014).Physiological response mechanism of three kinds of Acer rubrum L.under continuous high temperature and drought stress.Acta Ecol Sin,34(22):6471-6480(in Chinese with English abstract)[李力,刘玉民,王敏等(2014).3种北美红枫对持续高温干旱胁迫的生理响应机制.生态学报,34(22):6471-6480]
Li Y,Sun MG,King YJ,et al(2006).Physiological and biochemical responses of Gleditsia sinensis seedlings to drought stress.J South China Agr Univ,27(3):66-69(in Chinese with English abstract)[李燕,孙明高,孔艳菊等(2006).皂角苗木对干旱胁迫的生理生化反应.华南农业大学学报,27(3):66-69]
Liu G,Li X,Liang Z,et al(2003).Technoiques of tissue culture and in vitro clonal propagation of Ilex latifolia.For Res,16(4):439-443(in Chinese)[刘根林,李晓储,梁珍海等(2003).优良保健树大叶冬青组培扩繁的研究.林业科学研究,16(4):439-443]
Liu HY,Liang ZS,Liu SM,et al(2007).Effect of progressive drying and rewatering on protective enzym activities and osmoregulatory molecules in leaves of Eucommia ulmoides seedling.J Northwest For Univ,22(3):55-59(in Chinese with English abstract)[刘红云,梁宗锁,刘淑明等(2007).持续干旱及复水对杜仲幼苗保护酶活性和渗透调节物质的影响.西北林学院学报,22(3):55-59]
Liu J,Zhu JK(1997).Proline accumulation and salt-stressinduced gene expression in a salt-hypersensitive mutant of Arabidopsis.Plant Physiol,114(2):591-596
Liu S,Qin Y,Du F(2003).Studies on chemical components in leaf of Ilex latifolia.China J Chin Mater Med,28(9):44-46(in Chinese)[刘韶,秦勇,杜方麓(2003).苦丁茶化学成分研究.中国中药杂志,28(9):44-46]
Lu S,Chen S,Chen S,et al(2003).Responses of proline content and activities of enzymes in warm-season turfgrasses to soil drought stress.Acta Hortic Sin,30(3):303-306(in Chinese)[卢少云,陈斯平,陈斯曼等(2003).三种暖季型草坪草在干旱条件下脯氨酸含量和抗氧化酶活性的变化.园艺学报,30(3):303-306]
Luo YL,Bi TJ,Su ZL,et al(2014).Physiological response of Kalanchoe tubiflora leaves to drogut stress and rewatering.J Trop Subtrop Bot,22(4):391-398(in Chinese with English abstract)[罗银玲,毕廷菊,苏志龙等(2014).棒叶落地生根对干旱与复水的生理响应.热带亚热带植物学报,22(4):391-398]
Pan X,Qiu Q,Li J,et al(2014).Physiological indexes of six plant species from the tibetan plateau under drought stress.Acta Ecol Sin,34(13):3558-3567(in Chinese with English abstract)[潘昕,邱权,李吉跃等(2014).干旱胁迫对青藏高原6种植物生理指标的影响.生态学报,34(13):3558-3567]
Seo HL,Baek SY,Lee EH,et al(2017).Liqustri lucidi fructus inhibits hepatic injury and functions as an antioxidant by activation of AMP-activated protein kinase in vivo and in vitro.Chemico-Biol Interact,262:57-68
Su H,Jin J,Zhu Z(2014).Effects of drought stress on physiological characteristics of Quercus rubra seedlings.JNortheast For Univ,42(8):34-39(in Chinese with English abstract)[苏寒之,金建邦,祝遵凌(2014).干旱胁迫对北美红栎幼苗生理特性的影响.东北林业大学学报,42(8):34-39]
Tang ZC(1983).Plant responses and adaptability to water stress.Plant Physiol Commun,(3):24-29(in Chinese with English abstract)[汤章城(1983).植物对水分胁迫的反应和适应性.I.抗逆性的一般概念和植物的抗涝性.植物生理学通讯,(3):24-29]
Tang ZC(1984).Plant proline accumulation and its meaning in stress condition.Plant Physiol Commun,(1):15-21(in Chinese with English abstract)[汤章城(1984).逆境条件下植物脯氨酸的累积及其可能的意义.植物生理学通讯,(1):15-21]
Wang BS(1988).Biological free radicals and membrane damage of plants.Plant Physiol Commun,(2):12-16(in Chinese with English abstract)[王宝山(1988).生物自由基与植物膜伤害.植物生理学通讯,(2):12-16]
Wang BX,Huang JC,Wang H,et al(1989).The correlation of proline accumulation and drought resistance in various plants under water stress condition.Plant Physiol J,(1):46-51(in Chinese with English abstract)[王邦锡,黄久常,王辉等(1989).不同植物在水分胁迫条件下脯氨酸的累积与抗旱性的关系.植物生理学报,(1):46-51]
Wang L,Wu J,Xia PY,et al(2011).Physiological responses of Ilex latifolia to low temperature and its cold tolerance.J South China Agr Univ,32(3):82-86(in Chinese with English abstract)[王宁,吴军,夏鹏云等(2011).大叶冬青对低温胁迫的生理响应及抗寒性分析.华南农业大学学报,32(3):82-86]
Wang QM,Xu XC,Wu SG,et al(2005).The effect of drought stress on the content of osmotic adjusting substance in leaves and cell membrane permeability of different soybean varieties in seedling period.Seed,24(8):9-12(in Chinese with English abstract)[王启明,徐心诚,吴诗光等(2005).干旱胁迫对不同大豆品种苗期叶片渗透调节物质含量和细胞膜透性的影响.种子,24(8):9-12]
Wang X,Huang J(2015).Principles and Techniques of Plant Physiological and Biochemical Experiments.3rd ed.Beijing:Higher Education Press,274-277(in Chinese)[王学奎,黄见良(2015).植物生理生化实验原理与技术.第3版.北京:高等教育出版社,274-277]
Wu Y,Guo S(2017).Physiological response of Chinese varieties of drought stress and evaluation of drought resistance.J Northeast For Univ,45(1):20-24,29(in Chinese with English abstract)[武燕奇,郭素娟(2017).5个板栗品种对干旱胁迫的生理响应及抗旱性评价.东北林业大学学报,45(1):20-24,29]
Xiao WJ,Yang WL,Gong ZH(2000).Processing techniques of fresh leaves of Ilex kudingcha C.J.Tseng.J Hunan Agr Univ,26(4):278-281(in Chinese with English abstract)[肖文军,杨伟丽,龚志华(2000).苦丁茶鲜叶加工技术研究.湖南农业大学学报,26(4):278-281]
Xie M,Wang Q,Xu D,et al(2015).Comparative study of the effects on colonic microbiota fermentation in vitro of extracts from Ilex kudingcha C.J.Tseng and 3,5-dicaffeoylquinic acid.J Food Sci,36(17):124-129(in Chinese with Englishabstract)[谢旻皓,王晴川,徐冬兰等(2015).苦丁茶冬青苦丁茶提取物与3,5-双咖啡酰奎尼酸对肠道微生物体外发酵的影响.食品科学,36(17):124-129]
Ye Q,Zuo HJ,Liao HY,et al(2013).Study on in vitro antimicrobial activity of L.purpurascents Y.C.Yang.Mod Prev Med,40(7):1320-1322(in Chinese with English abstract)[叶倩,左浩江,廖虹瑜等(2013).粗壮女贞提取物的体外抑菌作用研究.现代预防医学,40(7):1320-1322]
Yi F,Zhao XL,Peng Y,et al(2016).Genus llex L.:phytochemistry,ethnopharmacology,and pharmacology.Chin Herb Med,8(3):209-230
Zhang L,Wei L,Long P,et al(2017).Response of Trachycarpus fortunei seedlings from different provenances to drought stress and drought resistance evaluation.J Northeast For Univ,45(11):1-5(in Chinese with English abstract)[张兰,韦小丽,龙鹏等(2017).不同种源棕榈幼苗对干旱胁迫的响应及抗旱性评价.东北林业大学学报,45(11):1-5]
Zhang L,Wu J,Mei L,et al(2011).Saline tolerance of Camptotheca acuminata provenances and the index selection for saline tolerance.Sci Silvae Sin,47(11):66-72(in Chinese with English abstract)[张露婷,吴江,梅丽等(2011).喜树种源耐盐能力评价及耐盐指标筛选.林业科学,47(11):66-72]
Zhang MS,Xie B,Tan F,et al(2003).Relationship among soluble protein,chlorophyll and ATP in sweet potato under water stress with drought resistance.Sci Agric Sin,36(1):13-16(in Chinese with English abstract)[张明生,谢波,谈锋等(2003).甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究.中国农业科学,36(1):13-16]
Zhang WT,Xie FC,Wang HT,et al(2009).Physiological response of three garden plants to drought stress.J Zhejiang For Coll,26(2):182-187(in Chinese with English abstract)[张文婷,谢福春,王华田等(2009).3种园林灌木幼苗对干旱胁迫的生理响应.浙江林学院学报,26(2):182-187]
Zhang YT(1994).Origin of kudingcha.J Syst Evol,32(1):100-102(in Chinese with English abstract)[张永田(1994).苦丁茶的原植物.植物分类学报,32(1):100-102]