桔梗种子发育过程中外观形态及生理生化的变化
|
摘要
以发育过程中的紫花桔梗和白花桔梗种子为试验材料,对其外观形态及生理生化指标的变化规律进行了研究,以期探明桔梗种子的适宜采收期。结果表明:在种子发育过程中,其外观形态变化与生理生化变化有密切联系。随着种子不断发育成熟,种子颜色由浅至深,发芽率不断升高;含水量和相对电导率呈下降的趋势;POD、CAT、MDA均呈单峰曲线变化;SOD活性呈"上升—下降—上升"的趋势。在种子发育后期,大部分生理生化反应趋于稳定。61DAF紫花桔梗种子与58DAF白花桔梗种子的颜色为黑色、黑褐色等,表面具有与种子长轴平行的细长条纹理,且此时种子的发芽率最高,分别为72.33%和91.00%。试验结果表明,紫花桔梗与白花桔梗种子的适宜采收期分别为61DAF、58DAF左右。
In order to ascertain the suitable harvesting period of Platycodon grandiflorum seeds,taking the seeds of purple flower P.grandiflorumand white flower P.grandiflorumduring seed development as experimental materials,we studied it's change rules of the morphological,physiological and biochemical indexes.The results showed that the morphological changes of seeds were closely related to physiological and biochemical changes during seed development.With the development and maturation of seeds,the color of seeds changed from light to deep,and the germination rate increased.The water content and relative conductivity were decreasing.POD,CAT and MDA all show single peak curve changes.SOD activity showed a trend of"rising-descending-rising".Inthelatestage of seed development,most physiological and biochemical reactions tend to be stable.The color of 61 days after flowers(DAF)purple flower P.grandiflorum seed and 58 DAF white flower P.grandiflorumseed were black,dark brown and so on,and the surface had a slender grain parallel to the longaxisoftheseeds.Inthisperiod,the germination rate of seeds was the highest,rising to 72.33% and91.00%respectively.The experimental results showed that the suitable harvesting time of the seeds of purple flower P.grandiflorumand white flower P.grandiflorum were about 61 DAF and 58 DAF respectively.
In order to ascertain the suitable harvesting period of Platycodon grandiflorum seeds,taking the seeds of purple flower P.grandiflorumand white flower P.grandiflorumduring seed development as experimental materials,we studied it's change rules of the morphological,physiological and biochemical indexes.The results showed that the morphological changes of seeds were closely related to physiological and biochemical changes during seed development.With the development and maturation of seeds,the color of seeds changed from light to deep,and the germination rate increased.The water content and relative conductivity were decreasing.POD,CAT and MDA all show single peak curve changes.SOD activity showed a trend of"rising-descending-rising".Inthelatestage of seed development,most physiological and biochemical reactions tend to be stable.The color of 61 days after flowers(DAF)purple flower P.grandiflorum seed and 58 DAF white flower P.grandiflorumseed were black,dark brown and so on,and the surface had a slender grain parallel to the longaxisoftheseeds.Inthisperiod,the germination rate of seeds was the highest,rising to 72.33% and91.00%respectively.The experimental results showed that the suitable harvesting time of the seeds of purple flower P.grandiflorumand white flower P.grandiflorum were about 61 DAF and 58 DAF respectively.
引文
[1]刘自刚,张雁.KH2PO4和青霉素对桔梗种子发芽的影响[J].种子,2007,26(11):71-72.
[2]于营,郭靖,王志清,等.桔梗开花物候与生殖特征[J].吉林农业大学学报,2014,36(3):294-299.
[3]张新静,于营,朴向民,等.桔梗种子发育过程中脱水耐性与可溶性糖、可溶性蛋白的关系[J].河北农业大学学报,2017,40(6):14-20.
[4]韩建国,牛忠联.老芒麦种子发育过程中的生理生化变化[J].草地学报,2000,8(4):237-244.
[5]孙颖,王阿香,杨雪,等.侧金盏花种子发育过程中生理生化动态变化特性[J].东北林业大学学报,2015,43(7):35-37.
[6]张心慧.桔梗种子超干贮藏生理生化特性研究[D].吉林农业大学,2013.
[7]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.
[8]杨忠仁,张凤兰,郝丽珍,等.沙葱种子发育过程中生理生化变化规律[J].北方园艺,2014(15):5-9.
[9]Fraser J,Egli D B,Leggett J E.Pod and Seed Development in Soybean Cultivars with Differences in Seed Size[J].Agronomy Journal,1982,74(1):80-85.
[10]宋松泉,龙春林,殷寿华,等.种子的脱水行为及其分子机制[J].植物分类与资源学报,2003,25(4):465-479.
[11]闫晓娜,田敏,王彩霞.扇脉杓兰种子发育过程中的生理生化变化[J].林业科学研究,2015,28(6):851-857.
[12]韩智龙.华北驼绒藜种子败育生理学机制的研究[D].内蒙古农业大学,2008.
[13]齐永平.华中五味子种子发育生理生化动态研究[D].陕西师范大学,2009.
[14]宋金凤,李锋,王亚军,等.低温胁迫下外源有机酸对长白落叶松幼苗生理生化特性的影响[J].林业科学,2012,48(2):89-95.
[15]吴楚,王政权.外源水杨酸对水曲柳幼苗遭受冷害后抗光氧化能力的影响Ⅰ.对抗氧化酶活性的影响[J].湖北农学院学报,2002,22(4):303-308.
[16]颜世超,高荣岐,尹燕枰.银杏含水量变化与活性氧清除酶活性的关系[J].中国农学通报,2005,21(3):207-210.
[17]Niedzwiedzsiegien I,Bogatekleszczynska R,Come D,et al.Effects of drying rate on dehydration sensitivity of excised wheat seedling shoots as related to sucrose metabolism and antioxidant enzyme activities[J].Plant Science,2004,167(4):879-888.
[18]罗银玲,宋松泉,何惠英,等.玉米胚发育过程中脱水耐性的变化[J].植物分类与资源学报,2005,27(3):301-309.
[2]于营,郭靖,王志清,等.桔梗开花物候与生殖特征[J].吉林农业大学学报,2014,36(3):294-299.
[3]张新静,于营,朴向民,等.桔梗种子发育过程中脱水耐性与可溶性糖、可溶性蛋白的关系[J].河北农业大学学报,2017,40(6):14-20.
[4]韩建国,牛忠联.老芒麦种子发育过程中的生理生化变化[J].草地学报,2000,8(4):237-244.
[5]孙颖,王阿香,杨雪,等.侧金盏花种子发育过程中生理生化动态变化特性[J].东北林业大学学报,2015,43(7):35-37.
[6]张心慧.桔梗种子超干贮藏生理生化特性研究[D].吉林农业大学,2013.
[7]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.
[8]杨忠仁,张凤兰,郝丽珍,等.沙葱种子发育过程中生理生化变化规律[J].北方园艺,2014(15):5-9.
[9]Fraser J,Egli D B,Leggett J E.Pod and Seed Development in Soybean Cultivars with Differences in Seed Size[J].Agronomy Journal,1982,74(1):80-85.
[10]宋松泉,龙春林,殷寿华,等.种子的脱水行为及其分子机制[J].植物分类与资源学报,2003,25(4):465-479.
[11]闫晓娜,田敏,王彩霞.扇脉杓兰种子发育过程中的生理生化变化[J].林业科学研究,2015,28(6):851-857.
[12]韩智龙.华北驼绒藜种子败育生理学机制的研究[D].内蒙古农业大学,2008.
[13]齐永平.华中五味子种子发育生理生化动态研究[D].陕西师范大学,2009.
[14]宋金凤,李锋,王亚军,等.低温胁迫下外源有机酸对长白落叶松幼苗生理生化特性的影响[J].林业科学,2012,48(2):89-95.
[15]吴楚,王政权.外源水杨酸对水曲柳幼苗遭受冷害后抗光氧化能力的影响Ⅰ.对抗氧化酶活性的影响[J].湖北农学院学报,2002,22(4):303-308.
[16]颜世超,高荣岐,尹燕枰.银杏含水量变化与活性氧清除酶活性的关系[J].中国农学通报,2005,21(3):207-210.
[17]Niedzwiedzsiegien I,Bogatekleszczynska R,Come D,et al.Effects of drying rate on dehydration sensitivity of excised wheat seedling shoots as related to sucrose metabolism and antioxidant enzyme activities[J].Plant Science,2004,167(4):879-888.
[18]罗银玲,宋松泉,何惠英,等.玉米胚发育过程中脱水耐性的变化[J].植物分类与资源学报,2005,27(3):301-309.