脑复康宁胶囊的质量标准研究
|
摘要
目的建立脑复康宁胶囊的含量测定方法,为完善脑复康宁胶囊的质量标准提供理论依据。方法采用TLC法对脑复康宁胶囊的主要成分黄芪和天麻进行定性鉴别,采用HPLC法对三七有效成分人参皂苷Rb1进行含量测定。结果 TLC斑点清晰、分离度好、专属性强,阴性对照无干扰;人参皂苷Rb1在0.051 2～1.640 0mg·mL-1范围内与峰面积积分值呈良好的线性关系,平均回收率为99.40%,RSD值为1.59%(n=6)。结论该方法简便、准确、可靠,可作为脑复康宁胶囊的含量测定方法。
Objective To consummate the quality standard and provide theory evidence of Naofukangning Capsules by constituting the method for assaying Naofukangning Capsules.Methods TLC was employed to identify Astragalus root and Gastrodia elat.Ginsenoside Rb1,the effective component of Panax notoginseng,was determined by HPLC in the prescription of Naofukangning Capsules.Results TLC spots were clear,well-separated and specific without any interference.The linear range of ginsenoside Rbl was 0.051 2-1.640 0mg·mL-1 with an average recovery of 99.40%(RSD=1.59%,n=6).Conclusion The established standard is suitable for the quality control of Naofukangning Capsules.The determination method is simple,accurate and reliable,and could be used as the determination method for Naofukangning Capsules.
Objective To consummate the quality standard and provide theory evidence of Naofukangning Capsules by constituting the method for assaying Naofukangning Capsules.Methods TLC was employed to identify Astragalus root and Gastrodia elat.Ginsenoside Rb1,the effective component of Panax notoginseng,was determined by HPLC in the prescription of Naofukangning Capsules.Results TLC spots were clear,well-separated and specific without any interference.The linear range of ginsenoside Rbl was 0.051 2-1.640 0mg·mL-1 with an average recovery of 99.40%(RSD=1.59%,n=6).Conclusion The established standard is suitable for the quality control of Naofukangning Capsules.The determination method is simple,accurate and reliable,and could be used as the determination method for Naofukangning Capsules.
引文
[1]张小强,李英,吴云,等.芪桂痛风片的质量标准研究[J].中国新药杂志,2017,26(2):169-173.
[2]李雅静,孙辉,李文莉.补血益母颗粒质量标准的提高[J].中国药师,2018,21(3):501-503.
[3]翁燕,陆超,蔡云珊,等.益气活血颗粒质量标准研究[J].中国现代中药,2017,19(11):1625-1630.
[4]国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:302-303.
[5]陈超,严常开,朱丽玲,等.小儿汗停颗粒质量标准研究[J].中国药师,2017,20(11):2074-2077.
[6]李洁环,王洛临,郭鸣,等.鼻舒通窍合剂制备工艺的优化,2017,39(9):1825-1829.
[7]陈书慧,徐如冰,田效志,等.芪百益气浓缩丸提取工艺及质量标准研究[J].中医学报,2017,32(5):810-814,847.
[8]吴静澜,张永萍,刘莉.天麻灵芝颗粒的质量标准研究[J].贵阳中医学院学报,2016,38(5):13-18,7.
[9]樊莉,周世文.黄芪天麻胶囊的质量标准研究[J].中国药房,2013,24(35):3314-3317.
[10]杨玉军,孙爱萍,刘琪,等.天麻钩藤合剂质量标准的研究[J].中南药学,2018,16(2):228-232.
[11]孙冬梅,林晓燕,李素梅,等.复方芪麻胶囊的质量标准研究[J].中国药房,2017,28(18):2553-2556.
[12]霍花,侯佳宁,杨琳,等.镇痫康脑A胶囊的成分研究[J].药学服务与研究,2016,16(5):386-389.
[13]陈锋,张燕琴.补脑胶囊的定性定量方法研究[J].中南药学,2015,13(4):413-416.
[14]祝洪艳,张力娜,王国丽,等.高效液相色谱法同时测定人参健脾丸中人参皂苷Rg1、Re、Rb1和酸枣仁皂苷A、B的含量[J].中国药学杂志,2016,51(9):751-756.
[15]黄晓燕,罗时,李瑞莲,等.HPLC法测定血络通胶囊中人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量[J].中国药师,2017,20(12):2248-2250.
[16]罗镭.冠心丹参滴丸质量标准的研究[J].海峡药学,2017,29(6):52-54.
[17]于士龙,王思琪,崔思娇,等.辛芷通窍丸的质量标准研究[J].西北药学杂志,2018,33(2):167-170.
[18]肖会敏,雷美娜,何悦,等.清心安神桂仁茶的质量标准研究[J].西北药学杂志,2018,33(1):10-13.
[19]苏晓涛,李素霞.三七不同部位人参皂苷Rg1含量的比较[J].中国药师,2007,10(2):183-184.
[20]陈光勇,赵德林,陈婧,等.不同生长年限三七中人参皂苷Rg1、Rb1的含量比较[J].科学技术与工程,2014,14(2):14-16.
[2]李雅静,孙辉,李文莉.补血益母颗粒质量标准的提高[J].中国药师,2018,21(3):501-503.
[3]翁燕,陆超,蔡云珊,等.益气活血颗粒质量标准研究[J].中国现代中药,2017,19(11):1625-1630.
[4]国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015:302-303.
[5]陈超,严常开,朱丽玲,等.小儿汗停颗粒质量标准研究[J].中国药师,2017,20(11):2074-2077.
[6]李洁环,王洛临,郭鸣,等.鼻舒通窍合剂制备工艺的优化,2017,39(9):1825-1829.
[7]陈书慧,徐如冰,田效志,等.芪百益气浓缩丸提取工艺及质量标准研究[J].中医学报,2017,32(5):810-814,847.
[8]吴静澜,张永萍,刘莉.天麻灵芝颗粒的质量标准研究[J].贵阳中医学院学报,2016,38(5):13-18,7.
[9]樊莉,周世文.黄芪天麻胶囊的质量标准研究[J].中国药房,2013,24(35):3314-3317.
[10]杨玉军,孙爱萍,刘琪,等.天麻钩藤合剂质量标准的研究[J].中南药学,2018,16(2):228-232.
[11]孙冬梅,林晓燕,李素梅,等.复方芪麻胶囊的质量标准研究[J].中国药房,2017,28(18):2553-2556.
[12]霍花,侯佳宁,杨琳,等.镇痫康脑A胶囊的成分研究[J].药学服务与研究,2016,16(5):386-389.
[13]陈锋,张燕琴.补脑胶囊的定性定量方法研究[J].中南药学,2015,13(4):413-416.
[14]祝洪艳,张力娜,王国丽,等.高效液相色谱法同时测定人参健脾丸中人参皂苷Rg1、Re、Rb1和酸枣仁皂苷A、B的含量[J].中国药学杂志,2016,51(9):751-756.
[15]黄晓燕,罗时,李瑞莲,等.HPLC法测定血络通胶囊中人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量[J].中国药师,2017,20(12):2248-2250.
[16]罗镭.冠心丹参滴丸质量标准的研究[J].海峡药学,2017,29(6):52-54.
[17]于士龙,王思琪,崔思娇,等.辛芷通窍丸的质量标准研究[J].西北药学杂志,2018,33(2):167-170.
[18]肖会敏,雷美娜,何悦,等.清心安神桂仁茶的质量标准研究[J].西北药学杂志,2018,33(1):10-13.
[19]苏晓涛,李素霞.三七不同部位人参皂苷Rg1含量的比较[J].中国药师,2007,10(2):183-184.
[20]陈光勇,赵德林,陈婧,等.不同生长年限三七中人参皂苷Rg1、Rb1的含量比较[J].科学技术与工程,2014,14(2):14-16.