葛根、藤茶、玉米低聚肽复合组方对小鼠慢性酒精性肝损伤的保护作用
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摘要
研究葛根、藤茶、玉米低聚肽复合组方的解酒功能和保护肝脏活性。通过动物行为学实验,研究对比葛根、藤茶、玉米低聚肽单独使用及其联合使用的解酒效果,结果表明,与模型组比较,葛根、藤茶、玉米低聚肽复合组方能显著延长小鼠的醉酒时间至(55.14±20.97)min,缩短醒酒时间至(75.88±23.28)min(p<0.05),明显改善醉酒小鼠的行为学指标。通过建立小鼠慢性酒精性肝损伤模型,测定血清及肝组织生化指标,观察肝组织病理变化以及检测肝组织蛋白表达水平,研究复合组方的保肝作用,结果表明,与模型组比较,复合组方能够减轻酒精对小鼠肝细胞的损伤,显著使肝损伤模型小鼠血清中谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)活性及肝组织中丙二醛(malondialdehyde,MDA)含量分别降低至(17.09±6.26)U/L,(21.41±6.80)U/L和(2.46±0.31)nmol/mg,肝组织中还原型谷胱甘肽(glutathione reduced,GSH)含量及超氧化物歧化酶(superoxide dismutase,SOD)活性分别增加至(8.80±2.14)nmol/mg和(241.99±35.60)U/mg(p<0.05),改善肝组织病理形态变化,下调肝组织中NF-κB、TNF-α表达。这表明复合组方具有解酒保肝作用,其作用机理可能与抗氧化活性、抑制炎症相关信号有关。
The anti-alcohol and hepatoprotective effect of a composite of pueraria, Ampelopsis grossedentata(AG) and corn oligopeptides(COP) were investigated. The anti-alcohol effects of pueraria, AG and COP used alone or in combination on drunk mice were evaluated through animal behavior experiments. The results showed that the pueraria-AG-COP composite(PAC composite) could significantly(p < 0.05) extend the waking time for drunkenness(up to 55.14±20.97 min) and shorten the period of hangover(to 75.88±23.28 min) while improving significantly the behavioral indices of the drunk mice, as compared with the model group. In the established chronic alcohol-induced liver injury mouse model, the hepatoprotective effect of PAC composite was investigated through measuring the biochemical indices of serum and liver tissues in mice, observing the pathological changes in hepatic tissues and examining the protein expression in liver tissues. Compared with the model group, the PAC composite could alleviate alcohol-induced liver damage in liver-injured mice: reducing significantly(p < 0.05) the activities of ALT and AST(to 17.09±6.26 and 21.41±6.80 U/L, respectively) in serum and the content of malondialdehyde(MDA; 2.46±0.31 nmol/mg) in liver tissues, whilst increasing significantly(p < 0.05) the content of glutathione(GSH, to 8.80±2.14 nmol/mg) and activity of superoxide dismutase(SOD; to 241.99±35.60 U/mg) in liver tissues. The PAC composite could also combat the pathological changes in liver tissues while down-regulating the protein expression of hepatic NF-κB and TNF-α in the liver-injured mice. All these results indicated that the PAC composite exhibited anti-alcohol and hepatoprotective effects through working mechanisms associated with its antioxidant activity and inhibition of the inflammatory-related signaling.
The anti-alcohol and hepatoprotective effect of a composite of pueraria, Ampelopsis grossedentata(AG) and corn oligopeptides(COP) were investigated. The anti-alcohol effects of pueraria, AG and COP used alone or in combination on drunk mice were evaluated through animal behavior experiments. The results showed that the pueraria-AG-COP composite(PAC composite) could significantly(p < 0.05) extend the waking time for drunkenness(up to 55.14±20.97 min) and shorten the period of hangover(to 75.88±23.28 min) while improving significantly the behavioral indices of the drunk mice, as compared with the model group. In the established chronic alcohol-induced liver injury mouse model, the hepatoprotective effect of PAC composite was investigated through measuring the biochemical indices of serum and liver tissues in mice, observing the pathological changes in hepatic tissues and examining the protein expression in liver tissues. Compared with the model group, the PAC composite could alleviate alcohol-induced liver damage in liver-injured mice: reducing significantly(p < 0.05) the activities of ALT and AST(to 17.09±6.26 and 21.41±6.80 U/L, respectively) in serum and the content of malondialdehyde(MDA; 2.46±0.31 nmol/mg) in liver tissues, whilst increasing significantly(p < 0.05) the content of glutathione(GSH, to 8.80±2.14 nmol/mg) and activity of superoxide dismutase(SOD; to 241.99±35.60 U/mg) in liver tissues. The PAC composite could also combat the pathological changes in liver tissues while down-regulating the protein expression of hepatic NF-κB and TNF-α in the liver-injured mice. All these results indicated that the PAC composite exhibited anti-alcohol and hepatoprotective effects through working mechanisms associated with its antioxidant activity and inhibition of the inflammatory-related signaling.
引文
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[16]潘兴昌,曾瑜,刘文颖,等.玉米低聚肽和姜黄素对小鼠的醒酒功能研究[J].食品与发酵工业,2016,42(11):25-34PAN Xing-chang,ZENG Yu,LIU Wen-ying,et al.Effects of corn oligo-peptides and curcumin on antialcoholism in mice[J].Food and Fermentation Industries,2016,42(11):25-34
[17]Guo Y,Zhao Q,Cao L,et al.Hepatoprotective effect of Gan Kang Yuan against chronic liver injury induced by alcohol[J].Journal of Ethnopharmacology,2017,208:1-7
[18]Li R,Xu L,Liang T,et al.Puerarin mediates hepatoprotection against CCl4-induced hepatic fibrosis rats through attenuation of inflammation response and amelioration of metabolic function[J].Food and Chemical Toxicology,2013,52:69-75
[19]Xie J,Liu J,Chen T M,et al.Dihydromyricetin alleviates carbon tetrachloride-induced acute liver injury via JNK-dependent mechanism in mice[J].World Journal of Gastroenterology,2015,21(18):5473-5481
[20]Wang S,Shi X L,Feng M,et al.Puerarin protects against CCl4-induced liver fibrosis in mice:Possible role of PARP-1inhibition[J].International Immunopharmacology,2016,38:238-245
[2]侯羽菲,陈吉.酒精性肝病治疗的新进展[J].世界最新医学信息文摘,2018,18(72):156-159HOU Yu-fei,CHEN Ji.New progress in the treatment of alcoholic liver disease[J].World Latest Medicine Information,2018,18(72):156-159
[3]张译文,李昱锦,胡冰芳,等.三种小鼠酒精性肝病短期模型的评价[J].药学学报,2018,53(2):236-243ZHANG Yi-wen,LI Yu-jin,HU Bing-fang,et al.Evaluation on three short-term animal models of alcoholic liver disease[J].Acta Pharmaceutica Sinica,2018,53(2):236-243
[4]Chen X,Li R,Liang T,et al.Puerarin improves metabolic function leading to hepatoprotective effects in chronic alcohol-induced liver injury in rats[J].Phytomedicine,2013,20(10):849-852
[5]Wei S Y,Chen Y,Xu X Y.Progress on the pharmacological research of puerarin:A review[J].Chinese Journal of Natural Medicines,2014,12(6):407-414
[6]朱振元,薛婧,刘晓翠,等.葛根素及葛根异黄酮对小鼠急性醉酒预防和解酒效果的研究[J].食品科学,2014,35(15):247-250ZHU Zhen-yuan,XUE Jing,LIU Xiao-cui,et al.Puerarin and isoflavones from kudzu root(Pueraria lobata ohwi)prevent acute drunkenness and relieve alcoholism in mice[J].Food Science,2014,35(15):247-250
[7]赵月蓉,侯碧玉,张莉,等.葛根素对实验性肝损伤的治疗作用研究进展[J].中国新药杂志,2017,26(9):1005-1010ZHAO Yue-rong,HOU Bi-yu,ZHANG Li,et al.Research progress of puerarin in the treatment of experimental liver injury[J].Chinese Journal of New Drugs,2017,26(9):1005-1010
[8]Shen Y,Lindemeyer A K,Gonzalez C,et al.Dihydromyricetin as a novel anti-alcohol intoxication medication[J].The Journal of Neuroscience,2012,32(1):390-401
[9]Qiu P,Dong Y,Li B,et al.Dihydromyricetin modulates p62and autophagy crosstalk with the Keap-1/Nrf2 pathway to alleviate ethanol-induced hepatic injury[J].Toxicology Letters,2017,274:31-41
[10]Li H,Li Q,Liu Z,et al.The versatile effects of dihydromyricetin in health[J].Evidence-based Complementary and Alternative Medicine,2017,6:1-10
[11]吴咖,陈红霞,黄仁彬,等.相思藤茶水提物的解酒作用[J].中国实验方剂学杂志,2012,18(4):187-189WU Ka,CHEN Hong-xia,HUANG Ren-bin,et al.Effect of xiangsi tengcha on dispelling alcoholic intoxication[J].Chinese Journal of Experimental Traditional Medical Formulae,2012,18(4):187-189
[12]谭沙,罗静,李建新,等.藤茶研究进展[J].安徽农业科学,2015,43(31):71-73TAN Sha,LUO Jing,LI Jian-xin,et al.Research progress of vine tea[J].Journal of Anhui Agricultural Sciences,2015,43(31):71-73
[13]Zhang F,Zhang J,Li Y.Corn oligopeptides protect against early alcoholic liver injury in rats[J].Food and Chemical Toxicology,2012,50(6):2149-2154
[14]Li H M,Guo P,Hu X,et al.Preparation of corn(Zea mays)peptides and their protective effect against alcohol-induced acute hepatic injury in NH mice[J].Biotechnology and Applied Biochemistry,2007,47(3):169-174
[15]刘雪姣.玉米低聚肽保肝作用的研究[D].镇江:江苏大学,2016LIU Xue-jiao.Study of corn oligopeptide on hepatoprotecive effect[D].Zhenjiang:Jiangsu University,2016
[16]潘兴昌,曾瑜,刘文颖,等.玉米低聚肽和姜黄素对小鼠的醒酒功能研究[J].食品与发酵工业,2016,42(11):25-34PAN Xing-chang,ZENG Yu,LIU Wen-ying,et al.Effects of corn oligo-peptides and curcumin on antialcoholism in mice[J].Food and Fermentation Industries,2016,42(11):25-34
[17]Guo Y,Zhao Q,Cao L,et al.Hepatoprotective effect of Gan Kang Yuan against chronic liver injury induced by alcohol[J].Journal of Ethnopharmacology,2017,208:1-7
[18]Li R,Xu L,Liang T,et al.Puerarin mediates hepatoprotection against CCl4-induced hepatic fibrosis rats through attenuation of inflammation response and amelioration of metabolic function[J].Food and Chemical Toxicology,2013,52:69-75
[19]Xie J,Liu J,Chen T M,et al.Dihydromyricetin alleviates carbon tetrachloride-induced acute liver injury via JNK-dependent mechanism in mice[J].World Journal of Gastroenterology,2015,21(18):5473-5481
[20]Wang S,Shi X L,Feng M,et al.Puerarin protects against CCl4-induced liver fibrosis in mice:Possible role of PARP-1inhibition[J].International Immunopharmacology,2016,38:238-245