桂科育成猪血常规指标的测定分析
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摘要
【目的】检测桂科育成猪的血常规指标,明确不同功能细胞(红细胞、白细胞和血小板)的数量变化及形态分布情况,为桂科猪的免疫评估与抗病性能研究提供基础数据。【方法】于49日龄起每周对44头桂科育成猪(公母各半)采血1次,连续采样7周,测定其血常规指标,并与长白猪和恩施黑猪的血常规指标进行比较分析。【结果】在桂科育成猪不同日龄的红细胞类检测指标中,红细胞计数(RBC)和血红蛋白含量(HGB)均在70日龄达最低值(6.85×1012/L和97.1 g/L),显著低于49、56、63、84和91日龄时的对应值(P<0.05,下同);平均红细胞血红蛋白(MCH)和平均血红蛋白浓度(MCHC)的变化规律基本一致,均在70日龄达最低值(15.11 pg和275.70 g/L)、91日龄达最高值(16.40 pg和314.30 g/L);红细胞压积(HCT)、平均红细胞体积(MCV)和红细胞体积分布宽度(RDW)的变化相对较稳定,不同日龄间均无显著差异(P>0.05,下同)。在白细胞类检测指标中,白细胞计数(WBC)于63日龄达最低值(13.71×10~9/L)、84日龄达最高值(17.52×10~9/L),二者差异显著;中性粒细胞计数(NEUT)在70日龄时达最低值(3.61×10~9/L),显著低于49和56日龄的对应值。淋巴细胞计数(LYMPH)的变化相对较稳定,受日龄增长的影响不显著。在血小板类检测指标中,血小板计数(PLT)、血小板压积(PCT)及血小板平均分布宽度(PDW)在不同日龄的变化相对较稳定,受日龄增长的影响不显著。与35日龄长白猪和5月龄以上恩施黑猪相比,桂科育成猪除HCT、平均血小板体积(MPV)、PCT和PDW等少数指标表现异常外,其他大部分血常规指标介于长白猪与恩施黑猪血常规指标范围内。【结论】桂科育成猪在血常规指标方面有一定的独特性,发育至49~91日龄时已具备较稳定的免疫系统。
【Objective】In this study,blood routine examination indexes of Guike pigs were detected,number variation and morphology distribution of different functional cells(red blood cells,white cells and platelets)were researched to provide basic reference data for Guike pig immunological evaluation and disease resistance research.【Method】Fortyfour healthy Guike pigs had been taken blood samples each week since 49 days old and lasted for seven weeks.Their blood routine examination indexes were measured,and compared with those of Landrace pigs and Enshi black pig.【Result】In red blood cells indexes of Guike pigs of different day ages,red blood cells(RBC) number and hemoglobin(HGB)content were the lowest(6.85×1012/L and 97.1 g/L)at the age of 70 days,significantly lower than the corresponding value at 49,56,63,84 and 91 days old(P<0.05,the same below).The rhythms of mean corpuscular hemoglobin(MCH)and mean corpuscular hemoglobin concentration(MCHC)were basically the same,both were the lowest(15.11 PG and 275.70 g/L)at the age of 70 days,and reached the highest value(16.40 pg and 314.30 g/L)at the age of 91 days.Hematokrit(HCT),mean corpuscular volume(MCV)and red blood cell distribution width(RDW)changed stablely,and there was no significant difference between different ages(P>0.05,the same below).In white blood cells indexes,the total number of white blood cells(WBC)at the age of 63 days was the lowest(13.71×10~9/L),reached the highest value(17.52×10~9/L)at the age of 84 days,there was significant difference between them.Neutrophils(NEUT)reached the the lowest(3.61×10~9/L)at the age of 70 days,significantly lower than the corresponding value of 49 and 56 days old.The change of total lymphocyte count(LYMPH)was relatively stable,it was not significantly affected by the increase of age.In platelet indexes,platelet(PLT)count,plateletcrit(PCT)and platelet distribution width(PDW)were relatively stable at different ages,and they were not significantly influenced by age.Compared with the 35 days old Landrace pigs and 5 months old Enshi black pigs,most of the blood routine indexes of Guike pigs were in between normal blood routine index values of Landrace pigs and enshi black pigs except for a few indexes:HCT,mean platelet volume(MPV),PCT and DW.【Conclusion】Guike pigs are peculiar in blood routine examination indexes.The pigs at 49-91 days old develop stable immune system.
【Objective】In this study,blood routine examination indexes of Guike pigs were detected,number variation and morphology distribution of different functional cells(red blood cells,white cells and platelets)were researched to provide basic reference data for Guike pig immunological evaluation and disease resistance research.【Method】Fortyfour healthy Guike pigs had been taken blood samples each week since 49 days old and lasted for seven weeks.Their blood routine examination indexes were measured,and compared with those of Landrace pigs and Enshi black pig.【Result】In red blood cells indexes of Guike pigs of different day ages,red blood cells(RBC) number and hemoglobin(HGB)content were the lowest(6.85×1012/L and 97.1 g/L)at the age of 70 days,significantly lower than the corresponding value at 49,56,63,84 and 91 days old(P<0.05,the same below).The rhythms of mean corpuscular hemoglobin(MCH)and mean corpuscular hemoglobin concentration(MCHC)were basically the same,both were the lowest(15.11 PG and 275.70 g/L)at the age of 70 days,and reached the highest value(16.40 pg and 314.30 g/L)at the age of 91 days.Hematokrit(HCT),mean corpuscular volume(MCV)and red blood cell distribution width(RDW)changed stablely,and there was no significant difference between different ages(P>0.05,the same below).In white blood cells indexes,the total number of white blood cells(WBC)at the age of 63 days was the lowest(13.71×10~9/L),reached the highest value(17.52×10~9/L)at the age of 84 days,there was significant difference between them.Neutrophils(NEUT)reached the the lowest(3.61×10~9/L)at the age of 70 days,significantly lower than the corresponding value of 49 and 56 days old.The change of total lymphocyte count(LYMPH)was relatively stable,it was not significantly affected by the increase of age.In platelet indexes,platelet(PLT)count,plateletcrit(PCT)and platelet distribution width(PDW)were relatively stable at different ages,and they were not significantly influenced by age.Compared with the 35 days old Landrace pigs and 5 months old Enshi black pigs,most of the blood routine indexes of Guike pigs were in between normal blood routine index values of Landrace pigs and enshi black pigs except for a few indexes:HCT,mean platelet volume(MPV),PCT and DW.【Conclusion】Guike pigs are peculiar in blood routine examination indexes.The pigs at 49-91 days old develop stable immune system.
引文
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潘天彪,张家富,肖正中,罗丽萍,何若钢,蓝海恩.2010.不同能量、蛋白对桂科母系猪生长性能及血液生化指标的影响[J].黑龙江畜牧兽医,(19):71-72.[Pan T B,Zhang J F,Xiao Z Z,Luo L P,He R G,Lan H E.2010.Effects of different energies and proteins on growth performance and blood biochemical indexes Guike maternal line pigs[J].Heilongjiang Animal Science and Veterinary Medicine,(19):71-72.]
田鹏鹏,朱丽莎,马青,艾彪,田甜.2017.红细胞压积与年龄以及红细胞沉降率的关系[J].国际检验医学杂志,38(2):209-210.[Tian P P,Zhu L S,Ma Q,Ai B,Tian T.2017.The relationship of HCT between age and ESR[J].International Journal of Laboratory Medicine,38(2):209-210.]
王怀中,林松,王彦平,武英,郭建凤,王继英.2014.品种和母猪对仔猪血常规和T淋巴细胞亚群性状的效应分析[J].畜牧兽医学报,45(11):1760-1766.[Wang H Z,Lin S,Wang Y P,Wu Y,Guo J F,Wang J Y.2014.Analyses of breeds and little effect on routine blood traits and T-lymphocyte subpopulation traits in piglets[J].Acta Veterinaria et Zootechnica Sinica,45(11):1760-1766.]
向宇,李宗明,李佳萍,刘国平,李长春,李新云,赵书红.2015.恩施黑猪血液指标测定分析及和CXCR4基因多态性的关联分析[J].猪业科学,32(7):130-132.[Xiang Y,Li Z M,Li J P,Liu G P,Li C C,Li X Y,Zhao S H.2015.Enshi black pig blood index measurement analysis and analysis of the correlation and CXCR4 gene polymorphism[J].Swine Industry Science,32(7):130-132.]
谢炳坤,黄光云,农素群,汪燕玲,覃倩,马青艳,秦谦涛,覃兆鲜,吴永绍,蓝海恩,潘天彪,黄敏瑞.2012.桂科Ⅰ号配套系猪生产性能的测定[C].长春:中国畜牧兽医学会养猪学分会五届三次理事会暨生猪产业科技创新发展论坛论文集:166-167.[Xie B K,Huang G Y,Nong S Q,Wang Y L,Qin Q,Ma Q Y,Qin Q T,Qin Z X,Wu Y S,Lan H E,Pan T B,Huang M R.2012.GuikeⅠsynthetic line pig production performance measurement[C].Changchun:Proceddings of the Third Meeting of Fifth Session of Pig Branch of China Association of Animal and Weterinary Medbicine·Development Forum on Swine Industry Science and Technology Innovation:166-167.]
Arosa F A,Pereira C F,Fonseca A M.2004.Red blood cells as modulators of T cell growth and survival[J].Current Pharmaceutical Design,10(2):191-201.
Beutler B.2004.Innate immunity:An overview[J].Molecular Immunology,40(12):845-859.
van de Vosse E,van Dissel J T,Ottenhoff T H.2009.Genetic deficiencies of innate immune signalling in human infectious disease[J].The Lancet.Infectious Diseases,9(11):688-698.
程泽信,倪心,韩有元,殷裕斌,唐登华.2007.家猪与家野杂交猪血液中八项指标的比较分析[J].湖北农业科学,46(3):419-421.[Cheng Z X,Ni X,Han Y Y,Yin Y B,Tang D H.2007.Comparison of eight blood indexes among domestic swine and two crossed wild boar[J].Hubei Agricultural Sciences,46(3):419-421.]
郭士琪,沈国顺,董烨,孙雪,赵一霏,李帅,荣柳,刘丽霞.2016.猪瘟、猪蓝耳病不同免疫程序对猪血液指标和料重比的影响[J].饲料工业,37(5):39-41.[Guo S Q,Shen G S,Dong Y,Sun X,Zhao Y F,Li S,Rong L,Liu L X.2016.Effect of different immune process of CSF and PRRS on blood index and ratio of feed to gain in pig[J].Feed Industry,37(5):39-41.]
李春燕,甘振磊,汤德元,陶玉顺,曾智勇,王彬,张晓杰,王凤.2012.中药添加剂对猪增重、血常规指标及免疫功能影响的研究[J].动物医学进展,33(10):53-56.[Li C Y,Gan Z L,Tang D Y,Tao Y S,Zeng Z Y,Wang B,Zhang X J,Wang F.2012.Effects of chinese herbal feed additives on weight gain,routine blood indexes and the level immunoglobulin in swine[J].Progress in Veterinary Medicine,33(10):53-56.]
刘江伟,张永久,李泽信,许永华,龙小平,赵笑云,李广生,常德从.2007.长白仔猪血常规及生化指标正常值探讨[J].中国比较医学杂志,17(7):393-394.[Liu J W,Zhang Y J,Li Z X,Xu Y H,Long X P,Zhao X Y,Li G S,Chang D C.2007.Investigation on the normal values of blood routine and serum biochemistry in Changbai piglet[J].Chinese Journal of Comparative Medicine,17(7):393-394.]
罗鲜青,杨翠,黄丽霞,何仁春,周俊华,梁琪妹,卢桂猷,杨曦颖,李绍波,杨家晃.2015.桂科猪肥育、屠宰性能及肉品性状分析(英文)[J].南方农业学报,46(1):133-137.[Luo X Q,Yang C,Huang L X,He R C,Zhou J H,Liang Q M,Lu G Y,Yang X Y,Li S B,Yang J H.2015.Analysis of fattening,slaughter performance and meat quality traits in Guike pig[J].Journal of Southern Agriculture,46(1):133-137.]
潘天彪,张家富,肖正中,罗丽萍,何若钢,蓝海恩.2010.不同能量、蛋白对桂科母系猪生长性能及血液生化指标的影响[J].黑龙江畜牧兽医,(19):71-72.[Pan T B,Zhang J F,Xiao Z Z,Luo L P,He R G,Lan H E.2010.Effects of different energies and proteins on growth performance and blood biochemical indexes Guike maternal line pigs[J].Heilongjiang Animal Science and Veterinary Medicine,(19):71-72.]
田鹏鹏,朱丽莎,马青,艾彪,田甜.2017.红细胞压积与年龄以及红细胞沉降率的关系[J].国际检验医学杂志,38(2):209-210.[Tian P P,Zhu L S,Ma Q,Ai B,Tian T.2017.The relationship of HCT between age and ESR[J].International Journal of Laboratory Medicine,38(2):209-210.]
王怀中,林松,王彦平,武英,郭建凤,王继英.2014.品种和母猪对仔猪血常规和T淋巴细胞亚群性状的效应分析[J].畜牧兽医学报,45(11):1760-1766.[Wang H Z,Lin S,Wang Y P,Wu Y,Guo J F,Wang J Y.2014.Analyses of breeds and little effect on routine blood traits and T-lymphocyte subpopulation traits in piglets[J].Acta Veterinaria et Zootechnica Sinica,45(11):1760-1766.]
向宇,李宗明,李佳萍,刘国平,李长春,李新云,赵书红.2015.恩施黑猪血液指标测定分析及和CXCR4基因多态性的关联分析[J].猪业科学,32(7):130-132.[Xiang Y,Li Z M,Li J P,Liu G P,Li C C,Li X Y,Zhao S H.2015.Enshi black pig blood index measurement analysis and analysis of the correlation and CXCR4 gene polymorphism[J].Swine Industry Science,32(7):130-132.]
谢炳坤,黄光云,农素群,汪燕玲,覃倩,马青艳,秦谦涛,覃兆鲜,吴永绍,蓝海恩,潘天彪,黄敏瑞.2012.桂科Ⅰ号配套系猪生产性能的测定[C].长春:中国畜牧兽医学会养猪学分会五届三次理事会暨生猪产业科技创新发展论坛论文集:166-167.[Xie B K,Huang G Y,Nong S Q,Wang Y L,Qin Q,Ma Q Y,Qin Q T,Qin Z X,Wu Y S,Lan H E,Pan T B,Huang M R.2012.GuikeⅠsynthetic line pig production performance measurement[C].Changchun:Proceddings of the Third Meeting of Fifth Session of Pig Branch of China Association of Animal and Weterinary Medbicine·Development Forum on Swine Industry Science and Technology Innovation:166-167.]
Arosa F A,Pereira C F,Fonseca A M.2004.Red blood cells as modulators of T cell growth and survival[J].Current Pharmaceutical Design,10(2):191-201.
Beutler B.2004.Innate immunity:An overview[J].Molecular Immunology,40(12):845-859.
van de Vosse E,van Dissel J T,Ottenhoff T H.2009.Genetic deficiencies of innate immune signalling in human infectious disease[J].The Lancet.Infectious Diseases,9(11):688-698.