锈毛莓总三萜超声提取工艺的优化及其抗氧化活性研究
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摘要
[目的]研究锈毛莓总三萜最佳提取工艺及其抗氧化活性。[方法]在单因素试验基础上,采用Box-Benhnke对影响锈毛莓总三萜提取率的4个因素进行优化分析,并通过对·OH、DPPH·和ABTS~+·的清除能力考察锈毛莓总三萜的体外抗氧化活性。[结果]在超声温度为80℃、料液比为1∶28(g∶mL)、超声时间为70 min、超声功率为400 W、乙醇体积分数为83%时,锈毛莓总三萜提取率为5.06%,与理论值偏差较小。随着样品浓度的增加,其抗氧化能力逐渐增强,其中对DPPH·和ABTS~+·的清除能力较强,半抑制浓度(IC_(50))分别为0.097和0.047 mg/mL;对·OH有较弱的清除能力。[结论]优选工艺适用于锈毛莓总三萜的提取,锈毛莓总三萜具有较好的抗氧化性。
[Objective] The research aimed to study the optimum extraction technology and antioxidant activity of total triterpenes from Rubus reflexus.[Method]Based on single-factor test, four independent factors were selected as affecting factors for Box-Beknken central composite experiment design. ·OH,DPPH· and ABTS~+· scavenging assays were carried out to study in vitro antioxidant activity of total triterpenes from Rubus reflexus.[Result] The optimal extraction technology was as follow: extraction temperature was 80 ℃, material-liquid ratio was 1︰28(g:mL),extraction time was 70 min, ultrasonic power was 400 W, ethanol concentration was 83%.Under these conditions, the extraction rate of total triterpenes was 5.06%, with a small deviation from the theoretical value. With the increase of the concentration of sample, its antioxidant ability gradually increased. Among them, the scavenging ability of DPPH· and ABTS~+·were more obvious, the half inhibiting concentrations(IC_(50)) were 0.097 and 0.047 mg/mL, the scavenging ability of ·OH was weaker. [Conclusion]The method is reasonable for the extraction of total triterpenes from Rubus reflexus, and the total triterpenes has a better anti-oxidation activity.
[Objective] The research aimed to study the optimum extraction technology and antioxidant activity of total triterpenes from Rubus reflexus.[Method]Based on single-factor test, four independent factors were selected as affecting factors for Box-Beknken central composite experiment design. ·OH,DPPH· and ABTS~+· scavenging assays were carried out to study in vitro antioxidant activity of total triterpenes from Rubus reflexus.[Result] The optimal extraction technology was as follow: extraction temperature was 80 ℃, material-liquid ratio was 1︰28(g:mL),extraction time was 70 min, ultrasonic power was 400 W, ethanol concentration was 83%.Under these conditions, the extraction rate of total triterpenes was 5.06%, with a small deviation from the theoretical value. With the increase of the concentration of sample, its antioxidant ability gradually increased. Among them, the scavenging ability of DPPH· and ABTS~+·were more obvious, the half inhibiting concentrations(IC_(50)) were 0.097 and 0.047 mg/mL, the scavenging ability of ·OH was weaker. [Conclusion]The method is reasonable for the extraction of total triterpenes from Rubus reflexus, and the total triterpenes has a better anti-oxidation activity.
引文
[1] 福建省三明市医学科学研究所.三明畲族民间医药[M].厦门:厦门大学出版社,2002:118.
[2] MEI Q X,CHEN X L,XIA X,et al.Isolation and chemotaxonomic significance of chemical constituents from Rubus parvifolius[J].Chinese herbal medicines,2016,8(1):75-79.
[3] CHEN P,YAN H J,MEI Q X,et al.Triterpenoids from the roots and stems of Rubus alceaefolius[J].Chemistry of natural compounds,2016,52(2):248-251.
[4] ONO M,YASUDA S,KOMATSU H,et al.Triterpenoids from the fruits and leaves of the blackberry(Rubus allegheniensis)and their inhibitory activities on foam cell formation in human monocyte-derived macrophage[J].Natural product research,2014,28(24):2347-2350.
[5] 康同辉,张一凡,解婉莹,等.超声波辅助提取灵芝三萜工艺研究[J].农业科技与装备,2017,23(6):41-43.
[6] 洪佳妮,徐先祥,何定峰,等.大孔树脂纯化七叶莲总三萜工艺研究[J].中华中医药杂志,2016(11):4811-4814.
[7] 程旺开,许月明,张冬冬.响应面优化黄秋葵叶多糖的提取工艺及其抗氧化活性考察[J].中国实验方剂学杂志,2017,23(4):38-42.
[8] 宋佳敏,王鸿飞,孙朦,等.响应面法优化金蝉花多糖提取工艺及抗氧化活性分析[J].食品科学,2018,39(4):275-281.
[9] 王新玲,王晓梅,胡君萍,等.新疆鼠尾草不同部位不同极性提取物抗氧化活性[J].中国实验方剂学杂志,2017,23(23):62-65.
[10] 赵玉静,李建宽,张鑫,等.番石榴叶黄酮类化学成分及其抗氧化活性研究[J].中国中药杂志,2018,43(4):760-765.
[11] 艾薇,李悦,杨晓娜,等.藿香叶黄酮提取工艺及体外抗氧化活性研究[J/OL].食品工业科技,2018-07-17[2018-10-12].http://kns.cnki.net/kcms/detail/11.1759.TS.20180716.1159.022.htm.
[12] 臧琛,聂其霞,王国华,等.Box-Behnken响应面法优化铁皮石斛的真空冷冻干燥工艺[J].中国实验方剂学杂志,2018,24(10):15-20.
[13] 林建原,季丽红.响应面优化银杏叶中黄酮的提取工艺[J].中国食品学报,2013,13(2):83-90.
[14] 肖怀秋,李玉珍,林亲录,等.响应面优化冷榨花生粕酶法制备多肽工艺的研究[J].中国粮油学报,2013,28(9):50-55.
[2] MEI Q X,CHEN X L,XIA X,et al.Isolation and chemotaxonomic significance of chemical constituents from Rubus parvifolius[J].Chinese herbal medicines,2016,8(1):75-79.
[3] CHEN P,YAN H J,MEI Q X,et al.Triterpenoids from the roots and stems of Rubus alceaefolius[J].Chemistry of natural compounds,2016,52(2):248-251.
[4] ONO M,YASUDA S,KOMATSU H,et al.Triterpenoids from the fruits and leaves of the blackberry(Rubus allegheniensis)and their inhibitory activities on foam cell formation in human monocyte-derived macrophage[J].Natural product research,2014,28(24):2347-2350.
[5] 康同辉,张一凡,解婉莹,等.超声波辅助提取灵芝三萜工艺研究[J].农业科技与装备,2017,23(6):41-43.
[6] 洪佳妮,徐先祥,何定峰,等.大孔树脂纯化七叶莲总三萜工艺研究[J].中华中医药杂志,2016(11):4811-4814.
[7] 程旺开,许月明,张冬冬.响应面优化黄秋葵叶多糖的提取工艺及其抗氧化活性考察[J].中国实验方剂学杂志,2017,23(4):38-42.
[8] 宋佳敏,王鸿飞,孙朦,等.响应面法优化金蝉花多糖提取工艺及抗氧化活性分析[J].食品科学,2018,39(4):275-281.
[9] 王新玲,王晓梅,胡君萍,等.新疆鼠尾草不同部位不同极性提取物抗氧化活性[J].中国实验方剂学杂志,2017,23(23):62-65.
[10] 赵玉静,李建宽,张鑫,等.番石榴叶黄酮类化学成分及其抗氧化活性研究[J].中国中药杂志,2018,43(4):760-765.
[11] 艾薇,李悦,杨晓娜,等.藿香叶黄酮提取工艺及体外抗氧化活性研究[J/OL].食品工业科技,2018-07-17[2018-10-12].http://kns.cnki.net/kcms/detail/11.1759.TS.20180716.1159.022.htm.
[12] 臧琛,聂其霞,王国华,等.Box-Behnken响应面法优化铁皮石斛的真空冷冻干燥工艺[J].中国实验方剂学杂志,2018,24(10):15-20.
[13] 林建原,季丽红.响应面优化银杏叶中黄酮的提取工艺[J].中国食品学报,2013,13(2):83-90.
[14] 肖怀秋,李玉珍,林亲录,等.响应面优化冷榨花生粕酶法制备多肽工艺的研究[J].中国粮油学报,2013,28(9):50-55.