艾纳香油对紫外线诱导小鼠皮肤晒伤的保护作用
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摘要
目的研究艾纳香油[Blumea balsamifera(L.)DC.,BBO]对紫外线诱导小鼠皮肤晒伤的保护作用及其作用机制。方法采用急性紫外线UVB辐射小鼠皮肤建立晒伤模型,创面外用艾纳香油,HE染色观察晒伤皮肤组织的病理变化,测定超氧化物岐化酶(SOD)活性及丙二醛(MDA)、还原型谷胱甘肽(GSH)含有量,利用酶联免疫吸附法(ELISA)检测表皮中8-羟基脱氧鸟嘌呤核苷(8-OHd G)、白细胞介素6(IL-6)及核转录因子-Kappa B(NF-κB)水平,免疫组化法检测增殖细胞核抗原(PCNA)、P53肿瘤抑制蛋白、肿瘤坏死因子(TNF-α)蛋白表达。结果与模型组比较,艾纳香油显著减少表皮厚度,使小鼠皮肤中SOD活性、GSH和MDA含有量得到恢复,显著降低8-OHd G、IL-6和NF-κB水平,同时抑制P53、PCNA表达。结论艾纳香油能够缓解UVB引起的皮肤晒伤,其机制与增强抗氧化作用,抑制NF-κB信号通路,下调IL-6释放,减少8-OHd G和PCNA水平有关。
AIM To study the protective effect of essential oils from Blumea balsamifera( L.) DC.( BBO)on UVB-induced sunburn in mouse skin and its mechanism of action. METHODS The model for sunburned mouse skin was established by acute UVB irradiation. Essential oils from B. balsamifera were applied to the surface of wound for external use. The pathological changes of sunburned skin tissue were observed by hematoxylin-eosin( HE) staining. The activity of superoxide dismutase( SOD),and the contents of malondialdehyde( MDA) and glutathione( GSH) were measured. The levels of 8-hydroxy-desoxyguanosine( 8-OHd G),interleukin-6( IL-6)and nuclear factor kappa-B( NF-κB) in epidermis were detected by ELISA. Additionally,the expressions of tumor necrosis factor-alpha( TNF-α),P53 tumor suppressor protein and proliferation cell nuclear antigen( PCNA) were evaluated by immunohistochemistry( IHC). RESULTS Compared with the model group,treatment with essential oils from B. balsamifera significantly reduced the thickness of epidermis,and the activity of SOD and the contents of MDA,GSH in mouse skin were restored. In addition,the essential oils from B. balsamifera resulted in a significant decrease in levels of 8-OHd G,IL-6 and NF-κB,and an inhibition in expressions of P53 and PCNA.CONCLUSION The essential oils from B. balsamifera can alleviate UVB-induced sunburn. Its mechanism is related to enhanced antioxidant power,inhibited NF-κB signal passway,down-regulated release of IL-6 and reduced levels of 8-OHd G,PCNA.
AIM To study the protective effect of essential oils from Blumea balsamifera( L.) DC.( BBO)on UVB-induced sunburn in mouse skin and its mechanism of action. METHODS The model for sunburned mouse skin was established by acute UVB irradiation. Essential oils from B. balsamifera were applied to the surface of wound for external use. The pathological changes of sunburned skin tissue were observed by hematoxylin-eosin( HE) staining. The activity of superoxide dismutase( SOD),and the contents of malondialdehyde( MDA) and glutathione( GSH) were measured. The levels of 8-hydroxy-desoxyguanosine( 8-OHd G),interleukin-6( IL-6)and nuclear factor kappa-B( NF-κB) in epidermis were detected by ELISA. Additionally,the expressions of tumor necrosis factor-alpha( TNF-α),P53 tumor suppressor protein and proliferation cell nuclear antigen( PCNA) were evaluated by immunohistochemistry( IHC). RESULTS Compared with the model group,treatment with essential oils from B. balsamifera significantly reduced the thickness of epidermis,and the activity of SOD and the contents of MDA,GSH in mouse skin were restored. In addition,the essential oils from B. balsamifera resulted in a significant decrease in levels of 8-OHd G,IL-6 and NF-κB,and an inhibition in expressions of P53 and PCNA.CONCLUSION The essential oils from B. balsamifera can alleviate UVB-induced sunburn. Its mechanism is related to enhanced antioxidant power,inhibited NF-κB signal passway,down-regulated release of IL-6 and reduced levels of 8-OHd G,PCNA.
引文
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[15]Lu Y P,Lou Y R,Li X H,et al.Stimulatory effect of oral administration of green tea or caffeine on ultraviolet light-induced increases in epidermal wild-type p53,p21(WAF1/CIP1),and apoptotic sunburn cells in SKH-1 mice[J].Cancer Res,2000,60(17):4785-4791.
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[2]Pang Y,Wang D,Fan Z,et al.Blumea balsamifera—A phytochemical and pharmacological review[J].Molecules,2014,19(7):9453-9477.
[3]吴丽芬,庞玉新,杨全,等.GC法同时测定艾纳香油中5个主要成分的含量[J].药物分析杂志,2015,35(7):1179-1184.
[4]Silva M A,Trevisan G,Hoffmeister C,et al.Anti-inflammatory and antioxidant effects of Aloe saponaria Haw in a model of UVB-induced paw sunburn in rats[J].J Photochem Photobiol B,2014,133:47-54.
[5]Zhao J G,Zhang Y Q.Inhibition of the flavonoid extract from silkworm cocoons on DMBA/UVB-induced skin damage and tumor promotion in BALB/c mice[J].Toxicol Res,2015,4(4):1016-1024.
[6]叶小红,宋洪涛.中药治疗日晒伤的研究进展[J].解放军药学学报,2010,26(1):83-86.
[7]Lee C W,Ko H H,Lin C C,et al.Artocarpin attenuates ultraviolet B-induced skin damage in hairless mice by antioxidant and anti-inflammatory effect[J].Food Chem Toxicol,2013,60:123-129.
[8]Fujii T,Okuda T,Yasui N,et al.Effects of amla extract and collagen peptide on UVB-induced photoaging in hairless mice[J].J Funct Foods,2013,5(1):451-459.
[9]王业秋,陈巧云,李建民,等.绿原酸对紫外线损伤的Ha Ca T细胞肿瘤坏死因子α和白细胞介素6表达的调节[J].中国药理学与毒理学杂志,2014,28(1):63-67.
[10]Schreck R,Albermann K A J,Baeuerle P A.Nuclear factorκB:an oxidative stress-responsive transcription factor of eukaryotic cells(a review)[J].Free Radic Res Commun,1992,17(4):221-237.
[11]Mc Nulty S E,Tohidian N B,Meyskens F L.Rel A,p50 and inhibitor of kappa B alpha are elevated in human metastatic melanoma cells and respond aberrantly to ultraviolet light B[J].Pigment Cell Res,2001,14(6):456-465.
[12]Xia X,Park E,Fischer S M,et al.Mouse genetic models reveal surprising functions of IκB kinase alpha in skin development and skin carcinogenesis[J].Cancers(Basel),2013,5(1):170-183.
[13]Rodríguez-Yanes E,Juarranz,Cuevas J,et al.Polypodium leucotomos decreases UV-induced epidermal cell proliferation and enhances p53 expression and plasma antioxidant capacity in hairless mice[J].Exp Dermatol,2012,21(8):638-640.
[14]骆丹,徐丽贤,缪旭,等.表没食子儿茶素没食子酸酯下调紫外线诱导小鼠皮肤TNF-αmRNA及P53蛋白水平的研究[J].中草药,2006,37(9):1367-1370.
[15]Lu Y P,Lou Y R,Li X H,et al.Stimulatory effect of oral administration of green tea or caffeine on ultraviolet light-induced increases in epidermal wild-type p53,p21(WAF1/CIP1),and apoptotic sunburn cells in SKH-1 mice[J].Cancer Res,2000,60(17):4785-4791.
[16]Kanao R,Masuda Y,Deguchi S,et al.Relevance of simultaneous mono-ubiquitinations of multiple units of PCNA homo-trimers in dna damage tolerance[J].PLo S One,2015,10(2):e0118775.
[17]Terra V A,Souza-Neto F P,Frade M A C,et al.Genistein prevents ultraviolet B radiation-induced nitrosative skin injury and promotes cell proliferation[J].J Photochem Photobiol B,2015,144:20-27.