王氏肝舒贴对肝郁血瘀证及急性肝损伤大鼠影响的研究
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摘要
目的评价王氏肝舒贴对肝郁血瘀证动物模型的血液流变学指标和血小板聚集率的影响以及对CCl_4诱发大鼠急性肝损伤的保护作用。方法①将Wistar大鼠随机分为5组,即空白对照组,肝郁血瘀组,王氏肝舒贴低剂量组(生药含量0.96 g/kg)、中剂量组(生药含量1.92 g/kg)、高剂量组(生药含量2.88 g/kg),每组8只。除空白对照组外,其余各组夹尾法复制大鼠肝郁血瘀模型。各组采取相应干预措施,14天后检测全血黏度、全血还原黏度、红细胞刚性指数、红细胞变形性指数和血小板聚集率。②将SD大鼠随机分为5组,即空白对照组,肝损伤组,王氏肝舒贴低剂量组(生药含量0.96 g/kg)、中剂量组(生药含量1.92 g/kg)、高剂量组(生药含量2.88 g/kg),雌雄各半,每组10只。除空白对照组外,其余各组复制CCl_4诱发的大鼠急性肝损伤模型。各组采取相应的干预措施,14天后检测肝病相关生化指标。结果①与肝郁血瘀组比较,王氏肝舒贴高剂量组对于夹尾法所致肝郁血瘀大鼠低切全血黏度、全血还原黏度,中切全血黏度、全血还原黏度,高切全血黏度、全血还原黏度的升高,具有显著的降低作用(P<0.05),对红细胞刚性指数、红细胞变形指数及红细胞聚集率有显著的降低作用(P<0.05)。②王氏肝舒贴中、高剂量组对CCl_4诱发的大鼠急性肝损伤导致的多项生化指标升高有显著降低作用(P<0.05)。结论王氏舒肝贴对肝郁血瘀证具有一定的疗效,并对于化学物质导致的肝损伤具有一定的保护作用。
Objective To evaluate the effects of Wangshi Ganshu patch on hemorheological indexes and platelet aggregation rate in the rat model of liver-depression and blood-stasis syndrome,and the protective effect for acute liver injury induced by CCl4 in rats. Methods(1) Wistar rats were randomly assigned into 5 groups,namely blank control group,liver-depression model group and the Wangshi Ganshu patch low-dose group( crude drug0.96 g/kg),medium-dose group( 1.92 g/kg) and high-dose group( 2.88 g/kg),8 rats in each group. Excluding the blank control group,rat model of liver-depression and blood-stasis was established using the " clip tail" method. Each group of rats was given the corresponding intervention. 14 days later,blood was collected to test the blood viscosity,blood reductive viscosity,erythrocyte rigidity index,erythrocyte deformation index and rate of platelet aggregation.(2)SD rats were randomly assigned into 5 groups,namely blank control group,hepatic injury model group,Wangshi Ganshu patch low-dose group( 0.96 g/kg),medium-dose group( 1.92 g/kg) and high-dose group( 2.88 g/kg),5 males and 5 females in each group. Excluding the blank control group,CCl4 was used to induce acute hepatic injury model of rats. Each group of rats received corresponding intervention and 14 days later, related biochemical indicators of liver disease were determined. Results(1)Compared with the liver-depression model group,in the high-dose group,increase in the low-cut blood viscosity and blood reductive viscosity,middle-cut blood viscosity and blood reductive viscosity as well as high-cut blood viscosity and blood reductive viscosity was significantly lowered in rat model of liver-depression and blood-stasis( P<0.05); and the erythrocyte rigidity index,erythrocyte deformation index and erythrocyte aggregation rate were reduced significantly( P<0.05).(2) Compared with the liverdepression model group,in the middle-dose and high-dose groups,multiple biochemical indicators of acute liver injury induced by CCl4 were lowered significantly( P<0.05). Conclusion Wangshi Ganshu patch has certain therapeutic effect in treating diseases with liver-depression and blood-stasis syndrome and shows protective effect on liver damage caused by chemical substances.
Objective To evaluate the effects of Wangshi Ganshu patch on hemorheological indexes and platelet aggregation rate in the rat model of liver-depression and blood-stasis syndrome,and the protective effect for acute liver injury induced by CCl4 in rats. Methods(1) Wistar rats were randomly assigned into 5 groups,namely blank control group,liver-depression model group and the Wangshi Ganshu patch low-dose group( crude drug0.96 g/kg),medium-dose group( 1.92 g/kg) and high-dose group( 2.88 g/kg),8 rats in each group. Excluding the blank control group,rat model of liver-depression and blood-stasis was established using the " clip tail" method. Each group of rats was given the corresponding intervention. 14 days later,blood was collected to test the blood viscosity,blood reductive viscosity,erythrocyte rigidity index,erythrocyte deformation index and rate of platelet aggregation.(2)SD rats were randomly assigned into 5 groups,namely blank control group,hepatic injury model group,Wangshi Ganshu patch low-dose group( 0.96 g/kg),medium-dose group( 1.92 g/kg) and high-dose group( 2.88 g/kg),5 males and 5 females in each group. Excluding the blank control group,CCl4 was used to induce acute hepatic injury model of rats. Each group of rats received corresponding intervention and 14 days later, related biochemical indicators of liver disease were determined. Results(1)Compared with the liver-depression model group,in the high-dose group,increase in the low-cut blood viscosity and blood reductive viscosity,middle-cut blood viscosity and blood reductive viscosity as well as high-cut blood viscosity and blood reductive viscosity was significantly lowered in rat model of liver-depression and blood-stasis( P<0.05); and the erythrocyte rigidity index,erythrocyte deformation index and erythrocyte aggregation rate were reduced significantly( P<0.05).(2) Compared with the liverdepression model group,in the middle-dose and high-dose groups,multiple biochemical indicators of acute liver injury induced by CCl4 were lowered significantly( P<0.05). Conclusion Wangshi Ganshu patch has certain therapeutic effect in treating diseases with liver-depression and blood-stasis syndrome and shows protective effect on liver damage caused by chemical substances.
引文
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[10] 刘长海,华春秀,归改霞,等.和厚朴酚对小鼠四氯化碳肝损伤的保护作用[J].郑州大学学报(医学版),2013,48(4):470- 473.
[11] 徐玉萍,王灵台.肝舒贴外敷治疗慢性肝病胁痛疗效观察及护理[J].实用肝脏病杂志,2006,9(5):310- 312.
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[2] 聂红明,王灵台.王灵台教授肝病辨证论治特色[J].上海中医药大学学报,2006,20(2):32- 34.
[3] 陈静,王灵台,赵钢.肝舒贴治疗慢性肝病胁痛的临床研究[J].上海中医药杂志,2004,38(10):6- 8.
[4] 顾立刚,古宇环.长期激怒刺激对大鼠全血黏度和巨噬细胞功能影响的研究[J].北京中医药大学学报,1997,20(2):28- 30.
[5] 季宁平,周莉江,严鑫,等.醋制香附不同提取部位对肝郁血瘀模型大鼠的影响[J].中药与临床,2017,8(2):57- 60.
[6] 孙喜波.乳宁汤对大鼠肝郁血瘀型乳腺非典型增生VEGF- A、bFGF、MVD表达的影响[D].济南:山东中医药大学,2004.
[7] 唐亚平,龚超奇,黄秋粤,等.逍遥散对肝郁脾虚证大鼠治疗作用的观察[J].西部中医药,2008,21(10):53- 54.
[8] 刘嵩,吕晓慧,孙宗喜,等.黄芩苷抗氧化活性及对四氯化碳致大鼠肝损伤的保护作用[J].中国药学,2015,24(8):538- 544.
[9] 张丛梅.绞股蓝多糖对四氯化碳所致大鼠肝损伤的保护作用[J].中国实验方剂学杂志,2013,19(1):244- 247.
[10] 刘长海,华春秀,归改霞,等.和厚朴酚对小鼠四氯化碳肝损伤的保护作用[J].郑州大学学报(医学版),2013,48(4):470- 473.
[11] 徐玉萍,王灵台.肝舒贴外敷治疗慢性肝病胁痛疗效观察及护理[J].实用肝脏病杂志,2006,9(5):310- 312.
[12] 赵钢,陈建杰.王灵台教授论述穴位敷贴透皮剂肝舒贴作用机制[J].中华中医药学刊,2003,21(6):846- 847.