铜绿假单胞菌群体感应系统研究进展
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摘要
群体感应系统是一种细胞密度依赖的基因表达系统,其广泛存在于细菌性病原体中,是细菌细胞通讯方式的一种。群体感应系统可利用细菌释放的信号分子不断监控周围细菌的密度。当细菌密度达到阈值时,群体感应系统网络将启动,参与调控生物被膜、细菌毒力等特定基因的表达,从而使临床抗感染治疗失败。而通过抑制群体感应系统,可一定程度上治疗铜绿假单胞菌引起的感染。本文通过查阅近年国内外相关文献,对铜绿假单胞菌群体感应系统研究进展进行总结,为临床铜绿假单胞菌治疗提供新的方向,即群体感应系统抑制剂有可能成为治疗铜绿假单胞菌感染的新策略。
Quorum sensing system is a kind of cell density-dependent gene expression system.It widely distributes in bacterial pathogens,and is one of the ways of bacterial cell-cell communication.Quorum sensing system can use the signal molecules relieved by bacteria to continuously monitor the density of surrounding bacteria.When the density of bacteria reaches the threshold,the quorum sensing system will activate to participate in the formation of biofilms and the expression of bacterial virulence factors,which results in the failure of anti-infection treatment.However,by inhibiting quorum sensing system,infections caused by Pseudomonas aeruginosa can be controlled to a certain extent.In this paper,the progress of research on quorum sensing system of Pseudomonas aeruginosain recent years were summarized,in order to provide a new direction for the clinical treatment of Pseudomonas aeruginosainfection.In other words,quorum sensing inhibitors may be a new strategy for the treatment of Pseudomonas aeruginosainfection.
Quorum sensing system is a kind of cell density-dependent gene expression system.It widely distributes in bacterial pathogens,and is one of the ways of bacterial cell-cell communication.Quorum sensing system can use the signal molecules relieved by bacteria to continuously monitor the density of surrounding bacteria.When the density of bacteria reaches the threshold,the quorum sensing system will activate to participate in the formation of biofilms and the expression of bacterial virulence factors,which results in the failure of anti-infection treatment.However,by inhibiting quorum sensing system,infections caused by Pseudomonas aeruginosa can be controlled to a certain extent.In this paper,the progress of research on quorum sensing system of Pseudomonas aeruginosain recent years were summarized,in order to provide a new direction for the clinical treatment of Pseudomonas aeruginosainfection.In other words,quorum sensing inhibitors may be a new strategy for the treatment of Pseudomonas aeruginosainfection.
引文
[1]HU Fupin,GUO Yan,ZHU Demei,et al.CHINET surveillance of bacterial resistance across China:Report of the results in 2016[J].Chin J Infect Chemother,2017,17(5):481-491.(in Chinese)胡付品,郭燕,朱德妹,等.2016年中国CHINET细菌耐药性监测[J].中国感染与化疗杂志,2017,17(5):481-491.
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[3]Lee J,Wu J,Deng Y,et al.A cell-cell communication signal integrates quorum sensing and stress response[J].Nat Chem Biol,2013,9(5):339-343.
[4]Weng L,Zhang Y,Yang Y,et al.Isolation of the autoinducer-quenching strain that inhibits LasR in Pseudomonas aeruginosa[J].Int J Mol Sci,2014,15(4):6328-6342.
[5]WANG Shiqi,HE Xiaojing,LI Xiaobing,et al.Research progress of quorum sensing system in Pseudomonas aeruginosa[J].Chin Hosp Pharm J,2016,36(2):149-152.(in Chinese)王诗琪,何晓静,李晓冰,等.铜绿假单胞菌群体感应系统研究进展[J].中国医院药学杂志,2016,36(2):149-152.
[6]Drees SL,Fetzner S.PqsE of Pseudomonas aeruginosa acts as pathway-specific thioesterase in the biosynthesis of alkylquinolone signaling molecules,2009to 2012[J].Int J Antimicrob Agents,2014,43(4):328-334.
[7]Kim HS,Lee SH,Byun Y,et al.6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition[J].Sci Rep,2015,5:8656.
[8]Lee J,Zhang L.The hierarchy quorum sensing network in Pseudomonas aeruginosa[J].Protein Cell,2015,6(1):26-41.
[9]Rampioni G,Bertani I,Pillai CR,et al.Functional characterization of the quorum sensing regulator RsaL in the plant-beneficial strain Pseudomonas putida WCS358[J].Appl Environ Microbiol,2012,78(3):726-734.
[10]Welsh MA,Eibergen NR,Moore JD,et al.Small molecule disruption of quorum sensing cross-regulation in Pseudomonas aeruginosa causes major and unexpected alterations to virulence phenotypes[J].J Am Chem Soc,2015,137(4):1510-1519.
[11]Bala A,Chhibber S,Harjai K.Pseudomonas quinolone signalling system:a component of quorum sensing cascade is a crucial player in the acute urinary tract infection caused by Pseudomonas aeruginosa[J].Int J Med Microbiol,2014,304(8):1199-1208.
[12]Zender M,Witzgall F,Drees SL,et al.Dissecting the Multiple Roles of PqsE in Pseudomonas aeruginosa Virulence by Discovery of Small Tool Compounds[J].ACS chemical biology,2016,28.
[13]孔晋亮.群体感应影响铜绿假单胞菌致病力的研究进展[J].医学信息,2013,26(2):371-372.
[14]Eibergen NR,Moore JD,Mattmann ME,et al.Potent and selective modulation of the RhlR quorum sensing receptor by using non-native ligands:An emerging target for virulence control in Pseudomonas aeruginosa[J].Chem Bio Chem,2015,16(16),2348-2356.
[15]Lawrence RM,Vincent MI,Kim L.Pseudomonas aeruginosa biofilms in disease[J].Microb Ecol,2014,68(1):1-12.
[16]唐佩,刘敏,林允照.铜绿假单胞菌生物被膜形成调控机制的研究进展[J].浙江预防医学,2015,27(1):48-51+54.
[17]Rasmussen TB,Givskov M.Quorum-sensing inhibitors as anti-pathogenic drugs[J].Int J Med Microbiol,2006,296(2/3):149-161.
[18]MENG Fei,LI Shuchang,YANG Wuning,et al.Research progress on biofilms in Pseudomonas aeruginosa[J].Chin Animal Husbandry Veter Med,2016,43(11):2976-2981.(in Chinese)孟非,黎书长,杨武宁,等.铜绿假单胞菌生物膜研究进展[J]中国畜牧兽医,2016,43(11):2976-2981.
[19]Zhao JM,Jiang HD,Chen W,et al.The role of quorum sensing system in antimicrobial induced ampC expression in Pseudomonas aeruginosa biofilm[J].J Basic Microbiol,2015,55(5):671-678.
[20]Tashiro Y,Yawata Y,Toyofuku M,et al.Interspecies interaction between Pseudomonas aeruginosaand other microorganisms[J].Microbes Environ,2013,28(1):13-24.
[21]ZENG Lili,SHI Daohua,NIU Peiguang.Research progress in quorum sensing system and its inhibitors of Pseudomonas aeruginosa[J].J Chin Antibiotics,2014,39(9):701-705+714.(in Chinese)曾莉莉,史道华,牛培广.铜绿假单胞菌群体感应系统及其抑制剂的研究进展[J].中国抗生素杂志,2014,39(9):701-705+714.
[22]Kalia VC.Quorum sensing inhibitors:An overview[J].Biotechnol Adv,2013,31(2):224-245.
[23]Hamida MA,Hoda MO,Rania A,et al.Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosaisolates in Egypt[J].J Infect Dev Ctries,2015,9(10):1091-1099.
[24]Manmohit K,Vivek KY,Pradeep KS,et al.Effect of cinnamon oil on quorum sensing-controlled virulence factors and biofilm formation in Pseudomonas aeruginosa[J].PLoS One,2015,10(8):e0135495.
[25]Packiavathy IA,Sasikumar P,Pandian SK,et al.Prevention of quorum-sensing-mediated biofilm development and virulence factors production in Vibrio spp.by curcumin[J].Appl Microbiol Biotechnol,2013,97(23):10177-10187.
[26]Sun WJ,Wang Y,Shen LX,et al.A novel screening system for Pseudomonas aeruginosa quorum sensing inhibitors[J].Chin J Biotech,2009,25(8):1173-1179.
[27]Kim SG,Yoon YH,Choi JW,et al.Effect of furanone on experimentally induced Pseudomonas aeruginosa biofilm formation:In vitro study[J].Int J Pediatr Otorhinolaryngol,2012,76(11):1575-1578.
[28]Kalia VC,Wood TK,Kumar P.Evolution of resistance to quorum-sensing inhibitors[J].Microb Ecol,2014,68(1):13-23.
[29]Rodolfo GC,Mariano MV,Norma VG,et al.Resistance to the quorum-quenching compounds brominated furanone C-30and 5-fluorouracil in Pseudomonas aeruginosa clinical isolates[J].Pathog Dis,2013,68(1):8-11.
[30]Maeda T,Garca-Contreras R,Pu M,et al.Quorum quenching quandary:Resistance to antivirulence compounds[J].ISME J,2012,6(30):493-501.
[31]陈向东,王素霞,汪辉,等.多尼培南对铜绿假单胞菌生物被膜形成和群体感应系统的影响[J].药学与临床研究,2016(3):193-197.
[32]Gupta P,Chhibber S,Harjai K.Subinhibitory concentration of ciprofloxacin targets quorum sensing system of Pseudomonas aeruginosacausing inhibition of biofilm formation&reduction of virulence[J].Indian J Med Res,2016,143(5):643-651.
[33]Imperi F,Massai F,Ramachandran-pillai C,et al.New life for an old drug:The anthelmintic drug niclosamide inhibits Pseudomonas aeruginosa quorum sensing[J].Antimicrob Agents Chemother,2013,57(2):996-1005.
[34]Mattmann ME,Blackwell HE.Small molecules that modulate quorum sensing and control virulence in Pseudomonas aeruginosa[J].J Org Chem,2010,75(20):6737-6746.
[35]Borlee BR,Geske GD,Blackwell HE,et al.Identification of synthetic inducers and inhibitors of the quorum-sensing regulator LasR in Pseudomonas aeruginosa by high-throughput screening[J].Appl Environ Microbiol,2010,76(24):8255-8258.
[36]Nizalapur S,Kimyon O,Biswas NN,et al.Design,synthesis and evaluation of N-aryl-glyoxamide derivatives as structurally novel bacterial quorum sensing inhibitors[J].Org Biomol Chem,2016,14(2):680-693.
[2]Sader HS,Farrell DJ,Robert K,et al.Antimicrobial susceptibility of Gram-negative organisms isolated from patients hospitalised with pneumonia in US and European hospitals:Results from the SENTRY antimicrobial surveillance program,2009-2012[J].Int J Antimicrob Agents,2014,43(4):328-334.
[3]Lee J,Wu J,Deng Y,et al.A cell-cell communication signal integrates quorum sensing and stress response[J].Nat Chem Biol,2013,9(5):339-343.
[4]Weng L,Zhang Y,Yang Y,et al.Isolation of the autoinducer-quenching strain that inhibits LasR in Pseudomonas aeruginosa[J].Int J Mol Sci,2014,15(4):6328-6342.
[5]WANG Shiqi,HE Xiaojing,LI Xiaobing,et al.Research progress of quorum sensing system in Pseudomonas aeruginosa[J].Chin Hosp Pharm J,2016,36(2):149-152.(in Chinese)王诗琪,何晓静,李晓冰,等.铜绿假单胞菌群体感应系统研究进展[J].中国医院药学杂志,2016,36(2):149-152.
[6]Drees SL,Fetzner S.PqsE of Pseudomonas aeruginosa acts as pathway-specific thioesterase in the biosynthesis of alkylquinolone signaling molecules,2009to 2012[J].Int J Antimicrob Agents,2014,43(4):328-334.
[7]Kim HS,Lee SH,Byun Y,et al.6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition[J].Sci Rep,2015,5:8656.
[8]Lee J,Zhang L.The hierarchy quorum sensing network in Pseudomonas aeruginosa[J].Protein Cell,2015,6(1):26-41.
[9]Rampioni G,Bertani I,Pillai CR,et al.Functional characterization of the quorum sensing regulator RsaL in the plant-beneficial strain Pseudomonas putida WCS358[J].Appl Environ Microbiol,2012,78(3):726-734.
[10]Welsh MA,Eibergen NR,Moore JD,et al.Small molecule disruption of quorum sensing cross-regulation in Pseudomonas aeruginosa causes major and unexpected alterations to virulence phenotypes[J].J Am Chem Soc,2015,137(4):1510-1519.
[11]Bala A,Chhibber S,Harjai K.Pseudomonas quinolone signalling system:a component of quorum sensing cascade is a crucial player in the acute urinary tract infection caused by Pseudomonas aeruginosa[J].Int J Med Microbiol,2014,304(8):1199-1208.
[12]Zender M,Witzgall F,Drees SL,et al.Dissecting the Multiple Roles of PqsE in Pseudomonas aeruginosa Virulence by Discovery of Small Tool Compounds[J].ACS chemical biology,2016,28.
[13]孔晋亮.群体感应影响铜绿假单胞菌致病力的研究进展[J].医学信息,2013,26(2):371-372.
[14]Eibergen NR,Moore JD,Mattmann ME,et al.Potent and selective modulation of the RhlR quorum sensing receptor by using non-native ligands:An emerging target for virulence control in Pseudomonas aeruginosa[J].Chem Bio Chem,2015,16(16),2348-2356.
[15]Lawrence RM,Vincent MI,Kim L.Pseudomonas aeruginosa biofilms in disease[J].Microb Ecol,2014,68(1):1-12.
[16]唐佩,刘敏,林允照.铜绿假单胞菌生物被膜形成调控机制的研究进展[J].浙江预防医学,2015,27(1):48-51+54.
[17]Rasmussen TB,Givskov M.Quorum-sensing inhibitors as anti-pathogenic drugs[J].Int J Med Microbiol,2006,296(2/3):149-161.
[18]MENG Fei,LI Shuchang,YANG Wuning,et al.Research progress on biofilms in Pseudomonas aeruginosa[J].Chin Animal Husbandry Veter Med,2016,43(11):2976-2981.(in Chinese)孟非,黎书长,杨武宁,等.铜绿假单胞菌生物膜研究进展[J]中国畜牧兽医,2016,43(11):2976-2981.
[19]Zhao JM,Jiang HD,Chen W,et al.The role of quorum sensing system in antimicrobial induced ampC expression in Pseudomonas aeruginosa biofilm[J].J Basic Microbiol,2015,55(5):671-678.
[20]Tashiro Y,Yawata Y,Toyofuku M,et al.Interspecies interaction between Pseudomonas aeruginosaand other microorganisms[J].Microbes Environ,2013,28(1):13-24.
[21]ZENG Lili,SHI Daohua,NIU Peiguang.Research progress in quorum sensing system and its inhibitors of Pseudomonas aeruginosa[J].J Chin Antibiotics,2014,39(9):701-705+714.(in Chinese)曾莉莉,史道华,牛培广.铜绿假单胞菌群体感应系统及其抑制剂的研究进展[J].中国抗生素杂志,2014,39(9):701-705+714.
[22]Kalia VC.Quorum sensing inhibitors:An overview[J].Biotechnol Adv,2013,31(2):224-245.
[23]Hamida MA,Hoda MO,Rania A,et al.Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosaisolates in Egypt[J].J Infect Dev Ctries,2015,9(10):1091-1099.
[24]Manmohit K,Vivek KY,Pradeep KS,et al.Effect of cinnamon oil on quorum sensing-controlled virulence factors and biofilm formation in Pseudomonas aeruginosa[J].PLoS One,2015,10(8):e0135495.
[25]Packiavathy IA,Sasikumar P,Pandian SK,et al.Prevention of quorum-sensing-mediated biofilm development and virulence factors production in Vibrio spp.by curcumin[J].Appl Microbiol Biotechnol,2013,97(23):10177-10187.
[26]Sun WJ,Wang Y,Shen LX,et al.A novel screening system for Pseudomonas aeruginosa quorum sensing inhibitors[J].Chin J Biotech,2009,25(8):1173-1179.
[27]Kim SG,Yoon YH,Choi JW,et al.Effect of furanone on experimentally induced Pseudomonas aeruginosa biofilm formation:In vitro study[J].Int J Pediatr Otorhinolaryngol,2012,76(11):1575-1578.
[28]Kalia VC,Wood TK,Kumar P.Evolution of resistance to quorum-sensing inhibitors[J].Microb Ecol,2014,68(1):13-23.
[29]Rodolfo GC,Mariano MV,Norma VG,et al.Resistance to the quorum-quenching compounds brominated furanone C-30and 5-fluorouracil in Pseudomonas aeruginosa clinical isolates[J].Pathog Dis,2013,68(1):8-11.
[30]Maeda T,Garca-Contreras R,Pu M,et al.Quorum quenching quandary:Resistance to antivirulence compounds[J].ISME J,2012,6(30):493-501.
[31]陈向东,王素霞,汪辉,等.多尼培南对铜绿假单胞菌生物被膜形成和群体感应系统的影响[J].药学与临床研究,2016(3):193-197.
[32]Gupta P,Chhibber S,Harjai K.Subinhibitory concentration of ciprofloxacin targets quorum sensing system of Pseudomonas aeruginosacausing inhibition of biofilm formation&reduction of virulence[J].Indian J Med Res,2016,143(5):643-651.
[33]Imperi F,Massai F,Ramachandran-pillai C,et al.New life for an old drug:The anthelmintic drug niclosamide inhibits Pseudomonas aeruginosa quorum sensing[J].Antimicrob Agents Chemother,2013,57(2):996-1005.
[34]Mattmann ME,Blackwell HE.Small molecules that modulate quorum sensing and control virulence in Pseudomonas aeruginosa[J].J Org Chem,2010,75(20):6737-6746.
[35]Borlee BR,Geske GD,Blackwell HE,et al.Identification of synthetic inducers and inhibitors of the quorum-sensing regulator LasR in Pseudomonas aeruginosa by high-throughput screening[J].Appl Environ Microbiol,2010,76(24):8255-8258.
[36]Nizalapur S,Kimyon O,Biswas NN,et al.Design,synthesis and evaluation of N-aryl-glyoxamide derivatives as structurally novel bacterial quorum sensing inhibitors[J].Org Biomol Chem,2016,14(2):680-693.