铜绿假单胞菌的群体感应系统及其抑制剂研究进展
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摘要
群体感应系统(quorum sensing system, QS)是一种微生物细胞与细胞间的交流系统。铜绿假单胞菌是该系统的典型代表,可调控细菌产生对抗生素的耐药、形成生物膜、产生毒力因子,并且减弱宿主的免疫应答。群体感应系统抑制剂(quorum sensing inhibitors, QSIs)在不影响细菌生长的前提下可降低细菌的毒性,且增强细菌生物膜对抗生素治疗的敏感性,这些特点使QSIs成为目前抗感染领域的研发热点。本文就铜绿假单胞菌的群体感应系统及QSIs的研究进展进行了综述。
Quorum sensing system(QS) is a communication system between microbial cells. Pseudomonas aeruginosa is a typical representative of this system, which regulates bacterial resistance to antibiotics, formation of biofilm, production of virulence factors and attenuating the host immune response. Without affecting bacterial growth, quorum sensing inhibitors(QSIs) could reduce bacterial toxicity and enhance the sensitivity of bacterial biofilm to antibiotic therapy. These characteristics making QSIs to be a research hot spot in the current anti-infective field. This review summarizes the current research advance on quorum sensing system and QSIs of Pseudomonas aeruginosa.
Quorum sensing system(QS) is a communication system between microbial cells. Pseudomonas aeruginosa is a typical representative of this system, which regulates bacterial resistance to antibiotics, formation of biofilm, production of virulence factors and attenuating the host immune response. Without affecting bacterial growth, quorum sensing inhibitors(QSIs) could reduce bacterial toxicity and enhance the sensitivity of bacterial biofilm to antibiotic therapy. These characteristics making QSIs to be a research hot spot in the current anti-infective field. This review summarizes the current research advance on quorum sensing system and QSIs of Pseudomonas aeruginosa.
引文
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[4]杜艳芬.微生物群体感应系统的研究进展[J].国际检验医学杂志,2013,34(3):334-336.
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[9]Lee J,Zhang L.The hierarchy quorum sensing network in Pseudomonas aeruginosa[J].Prot Cell,2015,6(1):26.
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[25]Davies D G,Parsek M R,Pearson J P,et al.The involvement of cell-to-cell signals in the development of a bacterial biofilm[J].Science,1998,280(5361):295-298.
[26]Kjelleberg S,Molin S.Is there a role for quorum sensing signals in bacterial biofilms[J].Curr Opin Microbiol,2002,5(3):254-258.
[27]Davey M E,Caiazza N C,O'Toole G A.Rhamnolipid surfactant production affects biofilm architecture in PAO1[J].J Bacteriol,2003,185(3):1027-1036.
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[35]林丽华,余加林,芦起,等.大蒜素对铜绿假单胞菌生物膜群体密度感应系统调控毒力因子表达的影响[J].中国微生态学杂志,2009,21(6):481-484.
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[37]G?kals?n B,Aksoydan B,Erman B,et al.Reducing virulence and biofilm of Pseudomonas aeruginosa,by potential quorum sensing inhibitor carotenoid:Zeaxanthin[J].Microb Ecol,2017,74(2):466-473.
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[40]Heidari A,Noshiranzadeh N,Haghi F,et al.Inhibition of quorum sensing related virulence factors of Pseudomonas aeruginosa by pyridoxal lactohydrazone[J].Microb Pathog,2017,112:103-110.
[41]Kumar L,Chhibber S,Kumar R,et al.Zingerone silences quorum sensing and attenuates virulence of Pseudomonas aeruginosa[J].Fitoterapia,2015,102:84.
[42]El-Mowafy S A,Shaaban M I,Kh A E G.Sodium ascorbate as a quorum sensing inhibitor of Pseudomonas aeruginosa[J].J Appl Microbiol,2014,117(5):1388-99.
[43]Packiavathy I A,Priya S,Pandian S K,et al.Inhibition of biofilm development of uropathogens by curcumin-an antiquorum sensing agent from Curcuma longa[J].Food Chem,2014,148(3):453-460.
[44]Sun Y,Sun F,Feng W,et al.Hyperoside inhibits biofilm formation of Pseudomonas aeruginosa[J].Exp Ther Med,2017,14(2):1647.
[45]Singh V K,Mishra A,Jha B.Anti-quorum sensing and antibiofilm activity of delftia tsuruhatensis extract by attenuating the quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa[J].Front Cell Infect Microbiol,2017,7:337.
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[47]Ma L,Liu X,Liang H,et al.Effects of 14-alpha-lipoyl andrographolide on quorum sensing in Pseudomonas aeruginosa[J].Antimicrob Agents Chemother,2012,56(12):6088.
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[50]Bala A,Kumar R,Harjai K.Inhibition of quorum sensing in Pseudomonas aeruginosa by azithromycin and its effectiveness in urinary tract infections[J].J Med Microbiol,2011,60(3):300-306.
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[2]柯慧慧,牛永武,顾頔,等.真菌群体感应分子法尼醇及其作用机制[J].食品安全质量检测学报,2017,8(3):862-868.
[3]梁心琰,阮海华.细菌群体感应及其在病原菌防治中的应用[J].生物技术通报,2015,31(1):33-38.
[4]杜艳芬.微生物群体感应系统的研究进展[J].国际检验医学杂志,2013,34(3):334-336.
[5]徐芳,李军,段云飞,等.细菌群体感应信号网络及其应用[J].生物学杂志,2014,(4):77-81.
[6]Rasamiravaka T,El J M.Quorum-sensing mechanisms and bacterial response to antibiotics in P.aeruginosa[J].Curr Microbiol,2016,73(5):747-753.
[7]杨帆,张乐,盛哈蕾,等.铜绿假单胞菌中群体感应系统的研究进展[J].中国抗生素杂志,2012,37(1):16-20.
[8]Gupta R.Quorum sensing gene regulation in Pseudomonas aeruginosa[J].Dissertations Theses-Gradworks,2012.
[9]Lee J,Zhang L.The hierarchy quorum sensing network in Pseudomonas aeruginosa[J].Prot Cell,2015,6(1):26.
[10]Seed P C,Passador L,Iglewski B H.Activation of the Pseudomonas aeruginosa lasI gene by LasR and the Pseudomonas autoinducer PAI:An autoinduction regulatory hierarchy[J].J Bacteriol,1995,177(3):654-659.
[11]Skindersoe M E,Alhede M,Phipps R,et al.Effects of antibiotics on quorum sensing in Pseudomonas aeruginosa[J].Antimicrob Agents Chemother,2008,52(10):3648-3663.
[12]Mcknight S L,Iglewski B H,Pesci E C.The Pseudomonas quinolone signal regulates rhl quorum sensing in Pseudomonas aeruginosa[J].J Bacteriol,2000,182(10):2702.
[13]Lee J,Wu J,Deng Y,et al.A cell-cell communication signal integrates quorum sensing and stress response[J].Nat Chem Biol,2013,9(5):339.
[14]Kariminik A,Majid B S,Kheirkhah B.Pseudomonas aeruginosa quorum sensing modulates immune responses:an updated review article[J].Immunol Lett,2017,190:1.
[15]Pourmand M R,Sadighian H,Naderi M.Relation between expression of the las quorum-sensing system in clinical isolates of Pseudomonas aeruginosa and expression of efflux pump and ampC[J].J Med Bacteriol,2013,2(3,4):32-40.
[16]Tam V H,Chang K T,Abdelraouf K,et al.Prevalence,resistance mechanisms,and susceptibility of multidrugresistant bloodstream isolates of Pseudomonas aeruginosa[J].Antimicrobial Agents Chemother,2010,54(3):1160-1164.
[17]Yang S,Lopez C R,Zechiedrich E L.Quorum sensing and multidrug transporters in Escherichia coli[J].Proc Natl Acad Sci U S A,2006,103(7):2386-2391.
[18]Romero D,Traxler M F,López D,et al.Antibiotics as signal molecules[J].Chem Rev,2011,111(9):5492-5505.
[19]Xu G M.Relationships between the regulatory systems of quorum sensing and multidrug resistance[J].Front Microbiol,2016,7(114).
[20]饶敏,魏维,盛下放.铜绿假单胞菌的群体感应系统与其致病及耐药机制[J].世界临床药物,2011,32(6):337-341.
[21]Passador L,Cook J M,Gambello M J,et al.Expression of Pseudomonas aeruginosa virulence genes requires cell-tocell communication[J].Science,1993,260(5111):1127.
[22]Gloyne L S,Grant G D,Perkins A V,et al.Pyocyanininduced toxicity in A549 respiratory cells is causally linked to oxidative stress[J].Toxicol In Vitro,2011,25(7):1353.
[23]Déziel E,Gopalan S,Tampakaki A P,et al.The contribution of MvfR to Pseudomonas aeruginosa pathogenesis and quorum sensing circuitry regulation:Multiple quorum sensing-regulated genes are modulated without affecting lasRI,rhlRI or the production of N-acyl-L-homoserine lactones[J].Mol Microbiol,2005,55(4):998-1014.
[24]Dekimpe V,Deziel E.Revisiting the quorum-sensing hierarchy in Pseudomonas aeruginosa:the transcriptional regulator RhIR regulates LasR-specific factors[J].Microbiol,2009,155(Pt 3):712.
[25]Davies D G,Parsek M R,Pearson J P,et al.The involvement of cell-to-cell signals in the development of a bacterial biofilm[J].Science,1998,280(5361):295-298.
[26]Kjelleberg S,Molin S.Is there a role for quorum sensing signals in bacterial biofilms[J].Curr Opin Microbiol,2002,5(3):254-258.
[27]Davey M E,Caiazza N C,O'Toole G A.Rhamnolipid surfactant production affects biofilm architecture in PAO1[J].J Bacteriol,2003,185(3):1027-1036.
[28]刘琳,谭小娟,贾爱群.细菌群体感应与细菌生物被膜形成之间的关系[J].微生物学报,2012,52(3):271-278.
[29]Reen F J,seudomonas,quinolone signal(PQS),and its precursor HHQ,modulate interspecies and interkingdom behaviour[J].FEMS Microbiol Ecol,2011,77(2):413-428.
[30]郭俏.铜绿假单胞菌致病因子抑制剂及喹诺酮信号分子介导的调节途径研究[D].西安:西北大学,2013.
[31]Kalia V C,Purohit H J.Quenching the quorum sensing system:Potential antibacterial drug targets[J].Crit Rev Microbiol,2011,37:121-140
[32]陈垂继,周颖,陈中山,等.致病菌群体感应系统及其抑制剂的研究进展[J].华南国防医学杂志,2017,(4):286-288.
[33]Hentzer M,Riedel K,Rasmussen T B,et al.Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound[J].Microbiol,2002,148(1):87-102.
[34]Garcíacontreras R,Perézeretza B,Jassochávez R,et al.High variability in quorum quenching and growth inhibition by furanone C-30 in Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients[J].Pathog Dis,2015,73(6).
[35]林丽华,余加林,芦起,等.大蒜素对铜绿假单胞菌生物膜群体密度感应系统调控毒力因子表达的影响[J].中国微生态学杂志,2009,21(6):481-484.
[36]Smyth A R,Cifelli P M,Ortori C A,et al.Garlic as an inhibitor of Pseudomonas aeruginosa quorum sensing in cystic fibrosis--a pilot randomized controlled trial[J].Pediatr Pulmonol,2010,45(4):356.
[37]G?kals?n B,Aksoydan B,Erman B,et al.Reducing virulence and biofilm of Pseudomonas aeruginosa,by potential quorum sensing inhibitor carotenoid:Zeaxanthin[J].Microb Ecol,2017,74(2):466-473.
[38]Mohabi S,Kalantarneyestanaki D,Mansouri S.Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by quercus infectoria gall extracts[J].Iran J Microbiol,2017,9(1):26-32.
[39]Niu K,Min K,Jung H,et al.Leaf extracts of selected gardening trees can attenuate quorum sensing and pathogenicity of Pseudomonas aeruginosa,PAO1[J].Ind JMicrobiol,2017:1-10.
[40]Heidari A,Noshiranzadeh N,Haghi F,et al.Inhibition of quorum sensing related virulence factors of Pseudomonas aeruginosa by pyridoxal lactohydrazone[J].Microb Pathog,2017,112:103-110.
[41]Kumar L,Chhibber S,Kumar R,et al.Zingerone silences quorum sensing and attenuates virulence of Pseudomonas aeruginosa[J].Fitoterapia,2015,102:84.
[42]El-Mowafy S A,Shaaban M I,Kh A E G.Sodium ascorbate as a quorum sensing inhibitor of Pseudomonas aeruginosa[J].J Appl Microbiol,2014,117(5):1388-99.
[43]Packiavathy I A,Priya S,Pandian S K,et al.Inhibition of biofilm development of uropathogens by curcumin-an antiquorum sensing agent from Curcuma longa[J].Food Chem,2014,148(3):453-460.
[44]Sun Y,Sun F,Feng W,et al.Hyperoside inhibits biofilm formation of Pseudomonas aeruginosa[J].Exp Ther Med,2017,14(2):1647.
[45]Singh V K,Mishra A,Jha B.Anti-quorum sensing and antibiofilm activity of delftia tsuruhatensis extract by attenuating the quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa[J].Front Cell Infect Microbiol,2017,7:337.
[46]Alyousef H M,Ahmed A F,Alshabib N A,et al.Onion peel ethylacetate fraction and its derived constituent quercetin 4'-O-β-D glucopyranoside attenuates quorum sensing regulated virulence and biofilm formation[J].Front Microbiol,2017,8:1675.
[47]Ma L,Liu X,Liang H,et al.Effects of 14-alpha-lipoyl andrographolide on quorum sensing in Pseudomonas aeruginosa[J].Antimicrob Agents Chemother,2012,56(12):6088.
[48]肖骏,沈立新,赵琳,等.半枝莲对铜绿假单胞菌群体感应系统的抑制作用[J].中国抗生素杂志,2014,39(12):885-890.
[49]Luo J,Dong B,Wang K,et al.Baicalin inhibits biofilm formation,attenuates the quorum sensing-controlled virulence and enhances Pseudomonas aeruginosa clearance in a mouse peritoneal implant infection model[J].Plos One,2017,12(4):e0176883.
[50]Bala A,Kumar R,Harjai K.Inhibition of quorum sensing in Pseudomonas aeruginosa by azithromycin and its effectiveness in urinary tract infections[J].J Med Microbiol,2011,60(3):300-306.
[51]Roudashti S,Zeighami H,Mirshahabi H,et al.Synergistic activity of sub-inhibitory concentrations of curcumin with ceftazidime and ciprofloxacin against Pseudomonas aeruginosa quorum sensing related genes and virulence traits[J].World J Microbiol Biotechnol,2017,33(3):50.
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