细菌群体感应淬灭酶及其应用研究进展
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摘要
细菌群体感应(quorum sensing,QS)是细菌的一种密度依赖型信息交流机制。群体感应淬灭(quorum quenching,QQ)能够破坏细菌群体感应系统,阻断细菌的信息交流。本文中,笔者综述了群体感应淬灭酶——酰基高丝氨酸内酯酶(AHL内酯酶)的研究进展,总结了群体感应淬灭在植物病害防治、水产养殖、膜污染以及医学领域的应用研究,旨在为挖掘群体感应淬灭资源和群体感应淬灭的应用提供理论依据与指导。
Quorum sensing(QS)is a kind of density-dependent information exchange mechanism of bacteria.Quorum quenching(QQ)can destroy quorum sensing systems and block bacterial information exchange.This article reviews the quorum quenching enzymes acyl-homoserine lactonase,and summarizes the quorum quenching application in plant disease control,aquaculture,membrane contamination and medical fields.The review will provide helpful information for further exploration of quorum quenching resources and application in new area.
Quorum sensing(QS)is a kind of density-dependent information exchange mechanism of bacteria.Quorum quenching(QQ)can destroy quorum sensing systems and block bacterial information exchange.This article reviews the quorum quenching enzymes acyl-homoserine lactonase,and summarizes the quorum quenching application in plant disease control,aquaculture,membrane contamination and medical fields.The review will provide helpful information for further exploration of quorum quenching resources and application in new area.
引文
[1] 丁贤,孙威文,殷波,等.细菌群体感应淬灭的几种调控机制及其潜在应用[J].南方水产科学,2013,9(1):74-79.
[2] GRANDCLéMENT C,TANNIèRES M,MORéRA S,et al.Quorum quenching:role in nature and applied developments[J].FEMS Microbiol Rev,2016,40(1):86-116.
[3] DONG W W,ZHU J,GUO X,et al.Characterization of AiiK,an AHL lactonase,from Kurthia huakui LAM0618T and its application in quorum quenching on Pseudomonas aeruginosa PAO1[J].Sci Rep,2018,8:6013.
[4] YAN Q,WU X G,WEI H L,et al.Differential control of the PcoI/PcoR quorum-sensing system in Pseudomonas fluorescens 2P24 by sigma factor RpoS and the GacS/GacA two-component regulatory system[J].Microbiol Res,2009,164(1):18-26.
[5] GUI M,WU R,LIU L,et al.Effects of quorum quenching by AHL lactonase on AHLs,protease,motility and proteome patterns in,Aeromonas veronii,LP-11[J].Int J Food Microbiol,2017,252:61-68.
[6] RASMUSSEN T B,GIVSKOV M.Quorum-sensing inhibitors as anti-pathogenic drugs[J].Int J Med Microbiol,2006,296(2):149-161.
[7] GERA C,SRIVASTAVA S.Quorum-sensing:the phenomenon of microbial communication[J].Curr Sci,2006,90(5):666-676.
[8] DONG Y H,WANG L H,XU J L,et al.Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase[J].Nature,2001,411:813-817.
[9] GARGE S S,NERURKAR A S.Attenuation of quorum sensing regulated virulence of Pectobacterium carotovorum sub sp.carotovorum through an AHL lactonase produced by Lysinibacillus sp.Gs50[J].PLoS ONE,2016,11(12):e0167344.
[10] PARK S Y,LEE S J,OH T K,et al.AhlD,an N-acylhomoserine lactonase in Arthrobacter sp.and predicted homologues in other bacteria[J].Microbiology,2003,149:1541-1450.
[11] ZHANG H B,WANG L H,ZHANG L H.Genetic control of quorum-sensing signal turnover in Agrobacterium tumefaciens[J].Proc Natl Acad Sci USA,2002,99(7):4638-4643.
[12] WANG W Z,MOROHOSHI T,SOMEYA N,et al.AidC,a novel N-acylhomoserine lactonase from the potato root-associated Cytophaga-Flavobacteria-Bacteroides(CFB)group bacterium Chryseobacterium sp.strain StRB126[J].Appl Environ Microbiol,2012,78(22):7985-7992.
[13] RIAZ K,ELMERICH C,RAFFOUX A,et al.Metagenomics revealed a quorum quenching lactonase QlcA from yet unculturable soil bacteria[J].Commun Agric Appl Biol Sci,2008,73(2):3-6.
[14] KRYSCIAK D,SCHMEISSER C,PREUSS S,et al.Involvement of multiple loci in quorum quenching of autoinducer I molecules in the nitrogen-fixing symbiont Rhizobium(Sinorhizobium)sp.strain NGR234[J].Appl Environ Microbiol,2011,77(15):5089-5099.
[15] SEE-TOO W S,EE R,LIM Y L,et al.AidP,a novel N-acyl homoserine lactonase gene from Antarctic Planococcus sp.[J].Sci Rep,2017,7:42968.
[16] MOROHOSHI T,TOMINAGA Y,SOMEYA N,et al.Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris[J].J Biosci Bioeng,2012,113(1):20-25.
[17] WANG W Z,MOROHOSHI T,IKENOYAM,et al.AiiM,a novel class of N-acyl homoserine lactonase from the leaf-associated bacterium Microbacterium testaceum[J].Appl Environ Microbiol,2010,76(8):2524-2530.
[18] MEI G Y,YAN X X,TURAK A,et al.AidH,an α/β-hydrolase fold family member from an Ochrobactrum sp.strain,is a novel N-acylhomoserine lactonase[J].Appl Environ Microbiol,2010,76(15):4933-4942.
[19] BAR-ROGOVSKY H,HUGENMATTER A,DAN S T.The evolutionary origins of detoxifying enzymes:the mammalian serum paraoxonases(PONs)relate to bacterial homoserine lactonases[J].J Biol Chem,2013,288(33):23914-23927.
[20] XUE B,CHOW J Y,BALDANSUREN A,et al.Correction to structural evidence of a productive active site architecture for an evolved quorum-quenching GKL lactonase[J].Biochemistry,2012,DOI:10.1021/bi3011829.
[21] CHOW J Y,WU L,WEN S Y.Directed evolution of a quorum-quenching lactonase from Mycobacterium avium subsp.paratuberculosis K-10 in the amidohydrolase superfamily[J].Biochemistry,2009,48(20):4344-4353.
[22] HONG K W,KOH C L,SAM C K,et al.Quorum quenching revisited:from signal decays to signalling confusion[J].Sensors,2012,12(4):4661-4696.
[23] HIBLOT J,GOTTHARD G,CHABRIERE E,et al.Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicus[J].PLoS ONE,2012,7(10):e47028.
[24] AFRIAT L,ROODVELDT C,MANCO G,et al.The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase[J].Biochemistry,2006,45(46):13677-13686.
[25] PARK S Y,HWANG B J,SHIN M H,et al.N-Acylhomoserine lactonase producing Rhodococcus spp.with different AHL-degrading activities[J].FEMS Microbiol Lett,2010,261(1):102-108.
[26] SCHIPPER C,HORNUNG C,BIJTENHOORN P,et al.Metagenome-derived clones encoding two novel lactonase family proteins involved in biofilm inhibition in Pseudomonas aeruginosa[J].Appl Environ Microbiol,2009,75(1):224-233.
[27] 邱健,李承光,贾振华,等.酰基高丝氨酸内酯酶SS10的酶学特性及其抗软腐病功能的初探[J].植物病理学报,2007,37(6):629-636.
[28] WANG L H,WENG L X,DONG Y H,et al.Specificity and enzyme kinetics of the quorum-quenching N-acyl homoserine lactone lactonase(AHL-lactonase)[J].J Biol Chem,2004,279(14):13645-13651.
[29] 柴鑫莉,周盈,林拥军,等.苏云金芽胞杆菌抗软腐病aiiA基因转花魔芋研究[J].分子植物育种,2007,5(5):613-618.
[30] ZHANG L,GUO Z,GAO H,et al.Interaction of Pseudostellaria heterophylla with quorum sensing and quorum quenching bacteria mediated by root exudates in a consecutive monoculture system[J].J Microbiol Biotechnol,2016,26(12):2159-2170.
[31] 温晓芳.aiiA基因在大肠杆菌中的表达及其活性检测[D].儋州:华南热带农业大学,2005.
[32] 张峰.N-酰基高丝氨酸内酯酶的原核表达、纯化及酶学特性研究[D].福州:福建师范大学,2009.
[33] 陈瑞东.N-酰基高丝氨酸内酯酶基因克隆、表达、性质研究与水产养殖应用[D].北京:中国农业科学院,2010.
[34] LEADBETTER J R,GREENBERG E P.Metabolism of acyl-homoserine lactone quorum-sensing signals by Variovorax paradoxus[J].J Bacteriol,2000,182(24):6921-6926.
[35] OCHIAI S,YASUMOTO S,MOROHOSHI T,et al.AmiE,a novel N-acylhomoserine lactone acylase belonging to the amidase family,from the activated-sludge isolate Acinetobacter sp.strain Ooi24[J].Appl Environ Microbiol,2014,80(22):6919-6925.
[36] LIN Y H,XU J L,HU J,et al.Acyl-homoserine lactone acylase from Ralstonia,strain XJ12B represents a novel and potent class of quorum-quenching enzymes[J].Mol Microbiol,2003,47(3):849-860.
[37] PARK S Y,KANG H O,JANG H S,et al.Identification of extracellular N-acylhomoserine lactone acylase from a Streptomyces sp.and its application to quorum quenching[J].Appl Environ Microbiol,2005,71(5):2632-2641.
[38] ROMERO M,DIGGLE S P,HEEB S,et al.Quorum quenching activity in Anabaena sp.PCC 7120:identification of AiiC,a novel AHL-acylase[J].FEMS Microbiol Lett,2010,280(1):73-80.
[39] CHOWDHARY P K,KESHAVAN N,NGUYEN H Q,et al.Bacillus megaterium CYP102A1 oxidation of acyl homoserine lactones and acyl homoserines[J].Biochemistry,2007,46(50):14429-14437.
[40] SIRI C,CHOON-KOOK S,KALAI M,et al.Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale(ginger)rhizosphere:co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia[J].BMC Microbiol,2011,11(1):51.
[41] TOTH I K,NEWTON J A,HYMAN L J,et al.Potato plants genetically modified to produce N-acylhomoserine lactones increase susceptibility to soft rot Erwiniae[J].Mol Plant Microbe Interact,2004,17(8):880-887.
[42] 张力群,田涛,梅桂英.群体感应淬灭:防治植物细菌病害的新策略[J].中国生物防治,2010,26(3):241-247.
[43] MOLINA L,CONSTANTINESCU F,MICHEL L,et al.Degradation of pathogen quorum-sensing molecules by soil bacteria:a preventive and curative biological control mechanism[J].FEMS Microbiol Ecol,2003,45(1):71-81.
[44] 曾慧.在蜡状芽胞杆菌中AHL内酯酶对ZwA抗软腐病的增强效果[D].武汉:华中农业大学,2006.
[45] NATRAH F M,RUWANDEEPIKA H A,PAWAR S,et al.Regulation of virulence factors by quorum sensing in Vibrio harveyi[J].Vet Microbiol,2011,154(1):124-129.
[46] BAI F,YIN H,CHEN J,et al.Disruption of quorum sensing in Vibrio harveyi by the AiiA protein of Bacillus thuringiensis[J].Aquaculture,2008,274(1):36-40.
[47] SONG Z F,FAN B,QIU J Q,et al.Bacillus Pumilus to disrupt the quorum sensing reduce the mortality of Gibel carp(Carassius auratus gibelio)caused by Aeromonas hydrophilia[C].Adv Mater Res,2014,1051:341-347.
[48] PONNUSAMY K,KAPPACHERY S,THEKEETTLE M,et al.Anti-biofouling property of vanillin on Aeromonas hydrophila initial biofilm on various membrane surfaces[J].World J Microbiol Biotechnol,2013,29(9):1695-1703.
[49] YEON K M,CHEONG W S,OH H S,et al.Quorum sensing:a new biofouling control paradigm in a membrane bioreactor for advanced wastewater treatment[J].Environ Sci Technol,2009,43(2):380-385.
[50] RAJESH P S,RAI V R.Inhibition of QS-regulated virulence factors in Pseudomonas aeruginosa,PAO1 and Pectobacterium carotovorum,by AHL-lactonase of endophytic bacterium Bacillus cereus,VT96[J].Biocatal Agric Biotechnol,2016,7:154-163.
[51] 王岩,于雅萌,张静静,等.海洋微生物群体感应与群体感应淬灭的开发利用[J].生物资源,2017(6):413-422.
[52] TOMASZ A.Control of the competent state in Pneumococcus by a hormone-like cell product:an example for a new type of regulatory mechanism in bacteria[J].Nature,1965,208:155-159.
[53] BJARNSHOLT T,GIVSKOV M.The role of quorum sensing in the pathogenicity of the cunning aggressor Pseudomonas aeruginosa[J].Anal Bioanal Chem,2007,387(2):409-414.
[54] BASAVARAJU M,SISNITY V S,PALAPARTHY R,et al.Quorum quenching:Signal jamming in dental plaque biofilms[J].J Dent Sci,2016,11(4):349-352.
[2] GRANDCLéMENT C,TANNIèRES M,MORéRA S,et al.Quorum quenching:role in nature and applied developments[J].FEMS Microbiol Rev,2016,40(1):86-116.
[3] DONG W W,ZHU J,GUO X,et al.Characterization of AiiK,an AHL lactonase,from Kurthia huakui LAM0618T and its application in quorum quenching on Pseudomonas aeruginosa PAO1[J].Sci Rep,2018,8:6013.
[4] YAN Q,WU X G,WEI H L,et al.Differential control of the PcoI/PcoR quorum-sensing system in Pseudomonas fluorescens 2P24 by sigma factor RpoS and the GacS/GacA two-component regulatory system[J].Microbiol Res,2009,164(1):18-26.
[5] GUI M,WU R,LIU L,et al.Effects of quorum quenching by AHL lactonase on AHLs,protease,motility and proteome patterns in,Aeromonas veronii,LP-11[J].Int J Food Microbiol,2017,252:61-68.
[6] RASMUSSEN T B,GIVSKOV M.Quorum-sensing inhibitors as anti-pathogenic drugs[J].Int J Med Microbiol,2006,296(2):149-161.
[7] GERA C,SRIVASTAVA S.Quorum-sensing:the phenomenon of microbial communication[J].Curr Sci,2006,90(5):666-676.
[8] DONG Y H,WANG L H,XU J L,et al.Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase[J].Nature,2001,411:813-817.
[9] GARGE S S,NERURKAR A S.Attenuation of quorum sensing regulated virulence of Pectobacterium carotovorum sub sp.carotovorum through an AHL lactonase produced by Lysinibacillus sp.Gs50[J].PLoS ONE,2016,11(12):e0167344.
[10] PARK S Y,LEE S J,OH T K,et al.AhlD,an N-acylhomoserine lactonase in Arthrobacter sp.and predicted homologues in other bacteria[J].Microbiology,2003,149:1541-1450.
[11] ZHANG H B,WANG L H,ZHANG L H.Genetic control of quorum-sensing signal turnover in Agrobacterium tumefaciens[J].Proc Natl Acad Sci USA,2002,99(7):4638-4643.
[12] WANG W Z,MOROHOSHI T,SOMEYA N,et al.AidC,a novel N-acylhomoserine lactonase from the potato root-associated Cytophaga-Flavobacteria-Bacteroides(CFB)group bacterium Chryseobacterium sp.strain StRB126[J].Appl Environ Microbiol,2012,78(22):7985-7992.
[13] RIAZ K,ELMERICH C,RAFFOUX A,et al.Metagenomics revealed a quorum quenching lactonase QlcA from yet unculturable soil bacteria[J].Commun Agric Appl Biol Sci,2008,73(2):3-6.
[14] KRYSCIAK D,SCHMEISSER C,PREUSS S,et al.Involvement of multiple loci in quorum quenching of autoinducer I molecules in the nitrogen-fixing symbiont Rhizobium(Sinorhizobium)sp.strain NGR234[J].Appl Environ Microbiol,2011,77(15):5089-5099.
[15] SEE-TOO W S,EE R,LIM Y L,et al.AidP,a novel N-acyl homoserine lactonase gene from Antarctic Planococcus sp.[J].Sci Rep,2017,7:42968.
[16] MOROHOSHI T,TOMINAGA Y,SOMEYA N,et al.Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris[J].J Biosci Bioeng,2012,113(1):20-25.
[17] WANG W Z,MOROHOSHI T,IKENOYAM,et al.AiiM,a novel class of N-acyl homoserine lactonase from the leaf-associated bacterium Microbacterium testaceum[J].Appl Environ Microbiol,2010,76(8):2524-2530.
[18] MEI G Y,YAN X X,TURAK A,et al.AidH,an α/β-hydrolase fold family member from an Ochrobactrum sp.strain,is a novel N-acylhomoserine lactonase[J].Appl Environ Microbiol,2010,76(15):4933-4942.
[19] BAR-ROGOVSKY H,HUGENMATTER A,DAN S T.The evolutionary origins of detoxifying enzymes:the mammalian serum paraoxonases(PONs)relate to bacterial homoserine lactonases[J].J Biol Chem,2013,288(33):23914-23927.
[20] XUE B,CHOW J Y,BALDANSUREN A,et al.Correction to structural evidence of a productive active site architecture for an evolved quorum-quenching GKL lactonase[J].Biochemistry,2012,DOI:10.1021/bi3011829.
[21] CHOW J Y,WU L,WEN S Y.Directed evolution of a quorum-quenching lactonase from Mycobacterium avium subsp.paratuberculosis K-10 in the amidohydrolase superfamily[J].Biochemistry,2009,48(20):4344-4353.
[22] HONG K W,KOH C L,SAM C K,et al.Quorum quenching revisited:from signal decays to signalling confusion[J].Sensors,2012,12(4):4661-4696.
[23] HIBLOT J,GOTTHARD G,CHABRIERE E,et al.Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicus[J].PLoS ONE,2012,7(10):e47028.
[24] AFRIAT L,ROODVELDT C,MANCO G,et al.The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase[J].Biochemistry,2006,45(46):13677-13686.
[25] PARK S Y,HWANG B J,SHIN M H,et al.N-Acylhomoserine lactonase producing Rhodococcus spp.with different AHL-degrading activities[J].FEMS Microbiol Lett,2010,261(1):102-108.
[26] SCHIPPER C,HORNUNG C,BIJTENHOORN P,et al.Metagenome-derived clones encoding two novel lactonase family proteins involved in biofilm inhibition in Pseudomonas aeruginosa[J].Appl Environ Microbiol,2009,75(1):224-233.
[27] 邱健,李承光,贾振华,等.酰基高丝氨酸内酯酶SS10的酶学特性及其抗软腐病功能的初探[J].植物病理学报,2007,37(6):629-636.
[28] WANG L H,WENG L X,DONG Y H,et al.Specificity and enzyme kinetics of the quorum-quenching N-acyl homoserine lactone lactonase(AHL-lactonase)[J].J Biol Chem,2004,279(14):13645-13651.
[29] 柴鑫莉,周盈,林拥军,等.苏云金芽胞杆菌抗软腐病aiiA基因转花魔芋研究[J].分子植物育种,2007,5(5):613-618.
[30] ZHANG L,GUO Z,GAO H,et al.Interaction of Pseudostellaria heterophylla with quorum sensing and quorum quenching bacteria mediated by root exudates in a consecutive monoculture system[J].J Microbiol Biotechnol,2016,26(12):2159-2170.
[31] 温晓芳.aiiA基因在大肠杆菌中的表达及其活性检测[D].儋州:华南热带农业大学,2005.
[32] 张峰.N-酰基高丝氨酸内酯酶的原核表达、纯化及酶学特性研究[D].福州:福建师范大学,2009.
[33] 陈瑞东.N-酰基高丝氨酸内酯酶基因克隆、表达、性质研究与水产养殖应用[D].北京:中国农业科学院,2010.
[34] LEADBETTER J R,GREENBERG E P.Metabolism of acyl-homoserine lactone quorum-sensing signals by Variovorax paradoxus[J].J Bacteriol,2000,182(24):6921-6926.
[35] OCHIAI S,YASUMOTO S,MOROHOSHI T,et al.AmiE,a novel N-acylhomoserine lactone acylase belonging to the amidase family,from the activated-sludge isolate Acinetobacter sp.strain Ooi24[J].Appl Environ Microbiol,2014,80(22):6919-6925.
[36] LIN Y H,XU J L,HU J,et al.Acyl-homoserine lactone acylase from Ralstonia,strain XJ12B represents a novel and potent class of quorum-quenching enzymes[J].Mol Microbiol,2003,47(3):849-860.
[37] PARK S Y,KANG H O,JANG H S,et al.Identification of extracellular N-acylhomoserine lactone acylase from a Streptomyces sp.and its application to quorum quenching[J].Appl Environ Microbiol,2005,71(5):2632-2641.
[38] ROMERO M,DIGGLE S P,HEEB S,et al.Quorum quenching activity in Anabaena sp.PCC 7120:identification of AiiC,a novel AHL-acylase[J].FEMS Microbiol Lett,2010,280(1):73-80.
[39] CHOWDHARY P K,KESHAVAN N,NGUYEN H Q,et al.Bacillus megaterium CYP102A1 oxidation of acyl homoserine lactones and acyl homoserines[J].Biochemistry,2007,46(50):14429-14437.
[40] SIRI C,CHOON-KOOK S,KALAI M,et al.Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale(ginger)rhizosphere:co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia[J].BMC Microbiol,2011,11(1):51.
[41] TOTH I K,NEWTON J A,HYMAN L J,et al.Potato plants genetically modified to produce N-acylhomoserine lactones increase susceptibility to soft rot Erwiniae[J].Mol Plant Microbe Interact,2004,17(8):880-887.
[42] 张力群,田涛,梅桂英.群体感应淬灭:防治植物细菌病害的新策略[J].中国生物防治,2010,26(3):241-247.
[43] MOLINA L,CONSTANTINESCU F,MICHEL L,et al.Degradation of pathogen quorum-sensing molecules by soil bacteria:a preventive and curative biological control mechanism[J].FEMS Microbiol Ecol,2003,45(1):71-81.
[44] 曾慧.在蜡状芽胞杆菌中AHL内酯酶对ZwA抗软腐病的增强效果[D].武汉:华中农业大学,2006.
[45] NATRAH F M,RUWANDEEPIKA H A,PAWAR S,et al.Regulation of virulence factors by quorum sensing in Vibrio harveyi[J].Vet Microbiol,2011,154(1):124-129.
[46] BAI F,YIN H,CHEN J,et al.Disruption of quorum sensing in Vibrio harveyi by the AiiA protein of Bacillus thuringiensis[J].Aquaculture,2008,274(1):36-40.
[47] SONG Z F,FAN B,QIU J Q,et al.Bacillus Pumilus to disrupt the quorum sensing reduce the mortality of Gibel carp(Carassius auratus gibelio)caused by Aeromonas hydrophilia[C].Adv Mater Res,2014,1051:341-347.
[48] PONNUSAMY K,KAPPACHERY S,THEKEETTLE M,et al.Anti-biofouling property of vanillin on Aeromonas hydrophila initial biofilm on various membrane surfaces[J].World J Microbiol Biotechnol,2013,29(9):1695-1703.
[49] YEON K M,CHEONG W S,OH H S,et al.Quorum sensing:a new biofouling control paradigm in a membrane bioreactor for advanced wastewater treatment[J].Environ Sci Technol,2009,43(2):380-385.
[50] RAJESH P S,RAI V R.Inhibition of QS-regulated virulence factors in Pseudomonas aeruginosa,PAO1 and Pectobacterium carotovorum,by AHL-lactonase of endophytic bacterium Bacillus cereus,VT96[J].Biocatal Agric Biotechnol,2016,7:154-163.
[51] 王岩,于雅萌,张静静,等.海洋微生物群体感应与群体感应淬灭的开发利用[J].生物资源,2017(6):413-422.
[52] TOMASZ A.Control of the competent state in Pneumococcus by a hormone-like cell product:an example for a new type of regulatory mechanism in bacteria[J].Nature,1965,208:155-159.
[53] BJARNSHOLT T,GIVSKOV M.The role of quorum sensing in the pathogenicity of the cunning aggressor Pseudomonas aeruginosa[J].Anal Bioanal Chem,2007,387(2):409-414.
[54] BASAVARAJU M,SISNITY V S,PALAPARTHY R,et al.Quorum quenching:Signal jamming in dental plaque biofilms[J].J Dent Sci,2016,11(4):349-352.